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Article ; Online: An Unusual Cause of Macroscopic T-Wave Alternans in a Patient With Acute Promyelocytic Leukemia.

Liang, Zhen-Hua / Zhao, Yun-Tao / Zhou, Yi-Chang

2021 Volume 181, Issue 7, Page(s) 985–987

MeSH term(s)	Arrhythmias, Cardiac/physiopathology ; Electrocardiography ; Humans ; Leukemia, Promyelocytic, Acute/physiopathology ; Male ; Middle Aged
Language	English
Publishing date	2021-05-17
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	2699338-7
ISSN	2168-6114 ; 2168-6106
ISSN (online)	2168-6114
ISSN	2168-6106
DOI	10.1001/jamainternmed.2021.1631
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Article ; Online: Two truxinate derivatives and a phenyldilactone from the leaves of

Wang, Ya-Feng / Pang, Nao / Liang, Zhen-Hua / Huang, Yong-Lin / He, Rui-Jie / Yang, Bing-Yuan / Liu, Zhang-Bin

Natural product research

2022 Volume 37, Issue 21, Page(s) 3605–3609

Abstract: Phytochemical investigation of the Leaves ... ...

Abstract	Phytochemical investigation of the Leaves of
Language	English
Publishing date	2022-07-04
Publishing country	England
Document type	Journal Article
ZDB-ID	2185747-7
ISSN	1478-6427 ; 1478-6419
ISSN (online)	1478-6427
ISSN	1478-6419
DOI	10.1080/14786419.2022.2095636
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Article: Isolation, culture, and identification of duck intestinal epithelial cells and oxidative stress model constructed

Zhang, Hao / Chen, Fang / Liang, Zhen-Hua / Wu, Yan / Pi, Jin-Song

In vitro cellular & developmental biology. 2019 Oct., v. 55, no. 9

2019

Abstract: Intestinal epithelial cells (IECs) not only have an absorption function but also act as a physical barrier between the body and the intestinal bacterial flora. Damage to IECs leads to the breakdown of this barrier and has negative effects on animal ... ...

Abstract	Intestinal epithelial cells (IECs) not only have an absorption function but also act as a physical barrier between the body and the intestinal bacterial flora. Damage to IECs leads to the breakdown of this barrier and has negative effects on animal health. Intestinal epithelial damage is frequently associated with long-term acute stress, such as increased temperature and new stress management models. The intestinal epithelial damage caused by environmental stress has been linked to oxidative stress. Until now, the effects of intestinal epithelial antioxidant activity from feed additives and treatments could be tested in ducks only in vivo because of the lack of in vitro cell culture systems. In this study, we describe our protocol for the easy isolation and culture of IECs from the small intestine of duck embryos. Immunofluorescence was used for the cytological identification of IECs. In addition, IEC marker genes (IAP and CDH1) could also be detected in cultured cells. And cell status assessments were performed, and cell proliferation viability was analyzed by CCK-8 assay. Furthermore, we constructed an oxidative stress model to be used to research the oxidative stress response mechanism, and drugs acting on the cell signal transduction pathway. In conclusion, we have developed an effective and rapid protocol for obtaining duck primary IECs and constructed an oxidative stress model. These IECs exhibit features consistent with epithelial cells and could be used to explore the physiological mechanisms of oxidative stress ex vivo.
Keywords	antioxidant activity ; bacteria ; cell culture ; cell proliferation ; cultured cells ; drugs ; ducks ; embryo (animal) ; epithelial cells ; feed additives ; fluorescent antibody technique ; genetic markers ; intestinal absorption ; intestinal mucosa ; models ; oxidative stress ; signal transduction ; small intestine ; stress management ; stress response ; temperature ; viability
Language	English
Dates of publication	2019-10
Size	p. 733-740.
Publishing place	Springer US
Document type	Article
ISSN	1071-2690
DOI	10.1007/s11626-019-00388-7
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Article ; Online: Isolation, culture, and identification of duck intestinal epithelial cells and oxidative stress model constructed.

Zhang, Hao / Chen, Fang / Liang, Zhen-Hua / Wu, Yan / Pi, Jin-Song

In vitro cellular & developmental biology. Animal

2019 Volume 55, Issue 9, Page(s) 733–740

Abstract	Intestinal epithelial cells (IECs) not only have an absorption function but also act as a physical barrier between the body and the intestinal bacterial flora. Damage to IECs leads to the breakdown of this barrier and has negative effects on animal health. Intestinal epithelial damage is frequently associated with long-term acute stress, such as increased temperature and new stress management models. The intestinal epithelial damage caused by environmental stress has been linked to oxidative stress. Until now, the effects of intestinal epithelial antioxidant activity from feed additives and treatments could be tested in ducks only in vivo because of the lack of in vitro cell culture systems. In this study, we describe our protocol for the easy isolation and culture of IECs from the small intestine of duck embryos. Immunofluorescence was used for the cytological identification of IECs. In addition, IEC marker genes (IAP and CDH1) could also be detected in cultured cells. And cell status assessments were performed, and cell proliferation viability was analyzed by CCK-8 assay. Furthermore, we constructed an oxidative stress model to be used to research the oxidative stress response mechanism, and drugs acting on the cell signal transduction pathway. In conclusion, we have developed an effective and rapid protocol for obtaining duck primary IECs and constructed an oxidative stress model. These IECs exhibit features consistent with epithelial cells and could be used to explore the physiological mechanisms of oxidative stress ex vivo.
MeSH term(s)	Animals ; Cell Proliferation/genetics ; Cell Survival/genetics ; Cells, Cultured ; Ducks/genetics ; Ducks/growth & development ; Epithelial Cells/cytology ; Epithelial Cells/metabolism ; Intestines/cytology ; Oxidative Stress/genetics ; Signal Transduction/genetics
Language	English
Publishing date	2019-08-05
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1077810-x
ISSN	1543-706X ; 0883-8364 ; 1071-2690
ISSN (online)	1543-706X
ISSN	0883-8364 ; 1071-2690
DOI	10.1007/s11626-019-00388-7
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Zs.A 851: Show issues

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Article: Plasma metabolites associated with physiological and biochemical indexes indicate the effect of caging stress on mallard ducks (Anas platyrhynchos).

Zheng, Chao / Wu, Yan / Liang, Zhen Hua / Pi, Jin Song / Cheng, Shi Bin / Wei, Wen Zhuo / Liu, Jing Bo / Lu, Li Zhi / Zhang, Hao

Animal bioscience

2021 Volume 35, Issue 2, Page(s) 224–235

Abstract: Objective: Cage rearing has critical implications for the laying duck industry because it is convenient for feeding and management. However, caging stress is a type of chronic stress that induces maladaptation. Environmental stress responses have been ... ...

Abstract	Objective: Cage rearing has critical implications for the laying duck industry because it is convenient for feeding and management. However, caging stress is a type of chronic stress that induces maladaptation. Environmental stress responses have been extensively studied, but no detailed information is available about the comprehensive changes in plasma metabolites at different stages of caging stress in ducks. We designed this experiment to analyze the effects of caging stress on performance parameters and oxidative stress indexes in ducks. Methods: Liquid chromatography tandem mass spectrometry (LC/MS-MS) was used to determine the changes in metabolites in duck plasma at 5 (CR5), 10 (CR10), and 15 (CR15) days after cage rearing and traditional breeding (TB). The associated pathways of differentially altered metabolites were analyzed using Kyoto encyclopedia of genes and genomes (KEGG) database. Results: The results of this study indicate that caging stress decreased performance parameters, and the plasma total superoxide dismutase levels were increased in the CR10 group compared with the other groups. In addition, 1,431 metabolites were detected. Compared with the TB group, 134, 381, and 190 differentially produced metabolites were identified in the CR5, CR10, and CR15 groups, respectively. The results of principal component analysis (PCA) show that the selected components sufficiently distinguish the TB group and CR10 group. KEGG analysis results revealed that the differentially altered metabolites in duck plasma from the CR5 and TB groups were mainly associated with ovarian steroidogenesis, biosynthesis of unsaturated fatty acids, and phenylalanine metabolism. Conclusion: In this study, the production performance, blood indexes, number of metabolites and PCA were compared to determine effect of the caging stress stage on ducks. We inferred from the experimental results that caging-stressed ducks were in the sensitive phase in the first 5 days after caging, caging for approximately 10 days was an important transition phase, and then the duck continually adapted.
Language	English
Publishing date	2021-08-25
Publishing country	Korea (South)
Document type	Journal Article
ISSN	2765-0189
ISSN	2765-0189
DOI	10.5713/ab.21.0241
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Article ; Online: DNA-Binding, Photocleavage, and Photodynamic Anti-cancer Activities of Pyridyl Corroles.

Liang, Zhen-Hua / Liu, Hai-Yang / Zhou, Rong / Zhang, Zao / Ali, Atif / Han, Bing-Jie / Liu, Yun-Jun / Xiao, Xin-Yan

The Journal of membrane biology

2016 Volume 249, Issue 4, Page(s) 419–428

Abstract: The DNA-binding, photocleavage, and antitumor activity of three free base pyridyl corroles 1, 2, and 3 have been investigated. The binding affinity toward CT-DNA decreases with increasing number of pentafluorophenyl, whereas the photocleavage activity ... ...

Abstract	The DNA-binding, photocleavage, and antitumor activity of three free base pyridyl corroles 1, 2, and 3 have been investigated. The binding affinity toward CT-DNA decreases with increasing number of pentafluorophenyl, whereas the photocleavage activity toward pBR322 DNA becomes more efficient. Singlet oxygen was demonstrated as active species responsible for DNA cleavage. These corroles exhibited high cytotoxicity against three tested cancer cells (Hela, HapG2, and A549) and the cytotoxicity could be further enhanced under irradiation. Intracellular reactive oxygen species level was also monitored using HeLa Cells upon the combined treatment of corroles and light. These corroles could be absorbed by HeLa cells at low concentration. They can induce the decrease of mitochondrial membrane potential and apoptosis of tumor cells under irradiation.
MeSH term(s)	Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Apoptosis/drug effects ; Cell Line, Tumor ; Cell Survival/drug effects ; DNA/chemistry ; DNA/metabolism ; DNA Cleavage/drug effects ; DNA Cleavage/radiation effects ; Humans ; Inhibitory Concentration 50 ; Light ; Membrane Potential, Mitochondrial/drug effects ; Molecular Structure ; Porphyrins/chemistry ; Porphyrins/pharmacology ; Reactive Oxygen Species/metabolism
Chemical Substances	Antineoplastic Agents ; Porphyrins ; Reactive Oxygen Species ; corrole ; DNA (9007-49-2)
Language	English
Publishing date	2016
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3082-x
ISSN	1432-1424 ; 0022-2631
ISSN (online)	1432-1424
ISSN	0022-2631
DOI	10.1007/s00232-016-9879-0
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Zs.A 613: Show issues

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Article: RbCa(2)Nb(3)O(10) from X-ray powder data.

Liang, Zhen-Hua / Tang, Kai-Bin / Chen, Qian-Wang / Zheng, Hua-Gui

Acta crystallographica. Section E, Structure reports online

2009 Volume 65, Issue Pt 6, Page(s) i44

Abstract: Rubidium dicalcium triniobate(V), RbCa(2)Nb(3)O(10), has been synthesized by solid-state reaction and its crystal structure refined from X-ray powder diffraction data using Rietveld analysis. The compound is a three-layer perovskite Dion-Jacobson phase ... ...

Abstract	Rubidium dicalcium triniobate(V), RbCa(2)Nb(3)O(10), has been synthesized by solid-state reaction and its crystal structure refined from X-ray powder diffraction data using Rietveld analysis. The compound is a three-layer perovskite Dion-Jacobson phase with the perovskite-like slabs derived by termination of the three-dimensional CaNbO(3) perovskite structure along the ab plane. The rubidium ions (4/mmm symmetry) are located in the inter-stitial space.
Language	English
Publishing date	2009-05-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	2041947-8
ISSN	1600-5368
ISSN	1600-5368
DOI	10.1107/S1600536809018157
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Article: Ruthenium(II) complexes: Cellular uptake, cytotoxicity, DNA-binding, photocleavage and antioxidant activity studies

Li, Zheng-Zheng / Liang, Zhen-Hua / Huang, Hong-Liang / Liu, Yun-Jun

Journal of molecular structure. 2011 Aug. 24, v. 1001, no. 1-3

2011

Abstract: Two new ruthenium(II) complexes [Ru(dmp)₂(DNPIP)]²⁺1 and [Ru(dmp)₂(DAPIP)]²⁺2 were synthesized and characterized. The DNA-binding behaviors of these complexes were investigated by absorption spectra, viscosity measurements and photocleavage. The DNA- ... ...

Abstract	Two new ruthenium(II) complexes [Ru(dmp)₂(DNPIP)]²⁺1 and [Ru(dmp)₂(DAPIP)]²⁺2 were synthesized and characterized. The DNA-binding behaviors of these complexes were investigated by absorption spectra, viscosity measurements and photocleavage. The DNA-binding constants for complexes 1 and 2 have been determined to be 6.24 (±0.11)×10⁴ and 1.64 (±0.49)×10⁴M⁻¹. The results suggest that complexes 1 and 2 intercalate between the DNA base pairs. Binding stoichiometries were studied through a luminescence-based Job plot. The major inflection points for complexes 1 and 2 at χ=0.31 and χ=0.51 were observed. The data were consistent with 2:1 and 1:1bp [complex]/[DNA] binding mode. Complex 1 shows higher activity than complex 2 against the selected tumor cell lines. In addition, the cellular uptake and antioxidant activity on hydroxyl radical were also explored.
Keywords	DNA ; absorption ; antioxidant activity ; cytotoxicity ; hydroxyl radicals ; ruthenium ; viscosity
Language	English
Dates of publication	2011-0824
Size	p. 36-42.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	194476-9
ISSN	0022-2860 ; 0377-046X
ISSN	0022-2860 ; 0377-046X
DOI	10.1016/j.molstruc.2011.06.011
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Zs.A 1205: Show issues

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Article ; Online: Cytotoxicity, apoptosis, cellular uptake, cell cycle arrest, photocleavage, and antioxidant activity of 1, 10-phenanthroline ruthenium(II) complexes.

Liu, Yun-Jun / Li, Zheng-Zheng / Liang, Zhen-Hua / Yao, Jun-Hua / Huang, Hong-Liang

DNA and cell biology

2011 Volume 30, Issue 10, Page(s) 839–848

Abstract: Two new ruthenium(II) complexes, [Ru(phen)2(DNPIP)](ClO4)2 (1) and [Ru(phen)2(DAPIP)](ClO4)2 (2), were synthesized and characterized. The DNA-binding properties of these complexes were investigated using UV/vis absorption titration, viscosity ... ...

Abstract	Two new ruthenium(II) complexes, [Ru(phen)2(DNPIP)](ClO4)2 (1) and [Ru(phen)2(DAPIP)](ClO4)2 (2), were synthesized and characterized. The DNA-binding properties of these complexes were investigated using UV/vis absorption titration, viscosity measurements, thermal denaturation, and photoactivated cleavage. The DNA binding constants for complexes 1 and 2 are 2.63 ± 0.25×10(5) M(-1) (s=2.45) and 1.51±0.18×10(5) M(-1) (s=1.34). The results indicated that these complexes interacted with DNA through the intercalative mode. The cytotoxicity in vitro of complexes 1 and 2 were assessed against BEL-7402, HepG-2, and MCF-7 cell lines by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was studied with the acridine orange/ethidium bromide staining method. The antiproliferative mechanism was explored with flow cytometry. Cellular uptake studies showed that complexes 1 and 2 can enter into the cytoplasm and accumulate in the nuclei. Cell cycle arrest and antioxidant activity were also investigated.
MeSH term(s)	Antineoplastic Agents/chemical synthesis ; Antineoplastic Agents/pharmacology ; Antioxidants/chemical synthesis ; Antioxidants/pharmacology ; Apoptosis/drug effects ; Breast Neoplasms/drug therapy ; Breast Neoplasms/pathology ; Cell Line, Tumor ; Cell Nucleus/metabolism ; Cell Survival/drug effects ; Cell Survival/radiation effects ; DNA/chemistry ; DNA/metabolism ; Electrophoretic Mobility Shift Assay ; Female ; Flow Cytometry ; Fluorescence ; Humans ; Hydroxyl Radical/antagonists & inhibitors ; Hydroxyl Radical/metabolism ; Intercalating Agents/chemical synthesis ; Intercalating Agents/pharmacology ; Kinetics ; Liver Neoplasms/drug therapy ; Liver Neoplasms/pathology ; Organometallic Compounds/chemical synthesis ; Organometallic Compounds/pharmacology ; Oxidation-Reduction/drug effects ; Oxidation-Reduction/radiation effects ; Phenanthrolines/chemistry ; Photolysis/radiation effects ; Plasmids/metabolism ; Ruthenium/chemistry ; Ruthenium/metabolism ; Spectrum Analysis ; Ultraviolet Rays
Chemical Substances	Antineoplastic Agents ; Antioxidants ; Intercalating Agents ; Organometallic Compounds ; Phenanthrolines ; Hydroxyl Radical (3352-57-6) ; Ruthenium (7UI0TKC3U5) ; DNA (9007-49-2) ; calf thymus DNA (91080-16-9)
Language	English
Publishing date	2011-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1024454-2
ISSN	1557-7430 ; 0198-0238 ; 1044-5498
ISSN (online)	1557-7430
ISSN	0198-0238 ; 1044-5498
DOI	10.1089/dna.2011.1243
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Article ; Online: Ruthenium(II) polypyridyl complexes: synthesis and studies of DNA binding, photocleavage, cytotoxicity, apoptosis, cellular uptake, and antioxidant activity.

Liu, Yun-Jun / Liang, Zhen-Hua / Li, Zheng-Zheng / Yao, Jun-Hua / Huang, Hong-Liang

DNA and cell biology

2011 Volume 30, Issue 10, Page(s) 829–838

Abstract: Two ruthenium (II) complexes [Ru(dmb)2(APIP)](ClO4)2 (APIP=2-(2-aminophenyl)imidazo[4,5-f ][1,10]phenanthroline, dmb=4,4'-dimethyl-2,2'-bipyridine; 1) and [Ru(dmb)2(HAPIP)](ClO4)2 (HAPIP=2-(2-hydroxyl-4-aminophenyl)imidazo[4,5-f ][1,10]phenanthroline; 2) ...

Abstract	Two ruthenium (II) complexes [Ru(dmb)2(APIP)](ClO4)2 (APIP=2-(2-aminophenyl)imidazo[4,5-f ][1,10]phenanthroline, dmb=4,4'-dimethyl-2,2'-bipyridine; 1) and [Ru(dmb)2(HAPIP)](ClO4)2 (HAPIP=2-(2-hydroxyl-4-aminophenyl)imidazo[4,5-f ][1,10]phenanthroline; 2) were synthesized and characterized. DNA binding was investigated by electronic absorption titration, luminescence spectra, thermal denaturation, viscosity measurements, and photocleavage. The DNA binding constants for complexes 1 and 2 were 4.20 (±0.14)×10(4) and 5.45 (±0.15)×10(4) M(-1). The results suggest that these complexes partially intercalate between the base pairs. The cytotoxicity of complexes 1 and 2 was evaluated by MTT assay. Cellular uptake was observed under fluorescence microscopy; complexes 1 and 2 can enter into the cytoplasm and accumulate in the nuclei. Apoptosis and the antioxidant activity against hydroxyl radicals (•OH) were also explored.
MeSH term(s)	2,2'-Dipyridyl/chemistry ; Antineoplastic Agents/chemical synthesis ; Antineoplastic Agents/pharmacology ; Antioxidants/chemical synthesis ; Antioxidants/pharmacology ; Apoptosis/drug effects ; Breast Neoplasms/drug therapy ; Breast Neoplasms/pathology ; Cell Line, Tumor ; Cell Nucleus/metabolism ; Cell Survival/drug effects ; Cell Survival/radiation effects ; DNA/chemistry ; DNA/metabolism ; Electrophoretic Mobility Shift Assay ; Female ; Fluorescence ; Humans ; Hydroxyl Radical/antagonists & inhibitors ; Hydroxyl Radical/metabolism ; Intercalating Agents/chemical synthesis ; Intercalating Agents/pharmacology ; Kinetics ; Liver Neoplasms/drug therapy ; Liver Neoplasms/pathology ; Organometallic Compounds/chemical synthesis ; Organometallic Compounds/pharmacology ; Oxidation-Reduction/drug effects ; Oxidation-Reduction/radiation effects ; Phenanthrolines/chemistry ; Photolysis/radiation effects ; Plasmids/metabolism ; Ruthenium/chemistry ; Ruthenium/metabolism ; Spectrum Analysis ; Ultraviolet Rays
Chemical Substances	Antineoplastic Agents ; Antioxidants ; Intercalating Agents ; Organometallic Compounds ; Phenanthrolines ; Hydroxyl Radical (3352-57-6) ; 2,2'-Dipyridyl (551W113ZEP) ; Ruthenium (7UI0TKC3U5) ; DNA (9007-49-2) ; calf thymus DNA (91080-16-9)
Language	English
Publishing date	2011-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1024454-2
ISSN	1557-7430 ; 0198-0238 ; 1044-5498
ISSN (online)	1557-7430
ISSN	0198-0238 ; 1044-5498
DOI	10.1089/dna.2010.1170
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