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Article ; Online: Chrysophanol, a main anthraquinone from Rheum palmatum L. (rhubarb), protects against renal fibrosis by suppressing NKD2/NF-κB pathway.

Gu, Mingjia / Zhou, Yufeng / Liao, Naikai / Wei, Qingxue / Bai, Zijun / Bao, Neng / Zhu, Ying / Zhang, Hang / Gao, Leiping / Cheng, Xiaolan

Phytomedicine : international journal of phytotherapy and phytopharmacology

2022 Volume 105, Page(s) 154381

Abstract: Purpose: Chronic kidney disease (CKD), characterized as renal dysfunction and multi-system damage, has become a serious public health problem with high prevalence and mortality. Rheum palmatum L. (rhubarb) is one of the most widely used Chinese herb ... ...

Abstract	Purpose: Chronic kidney disease (CKD), characterized as renal dysfunction and multi-system damage, has become a serious public health problem with high prevalence and mortality. Rheum palmatum L. (rhubarb) is one of the most widely used Chinese herb with renal protective activity. However, the active components and underlying mechanisms of rhubarb remain unknown. In this work, we tried to explore the pharmacological mechanism of chrysophanol, a main anthraquinone from rhubarb, against CKD by in vivo and in vitro models. Study design: The therapeutic effect of chrysophanol and its underlying mechanism were investigated using CKD mouse model induced by unilateral ureteral occlusion (UUO), and human kidney 2 (HK-2) cells stimulated by TGF-β1 in vivo. Methods: The impact of chrysophanol on renal function, inflammation, fibrosis of CKD mice were evaluated. Then, the protein expressions of FN1, collagen ɑI, α-SMA, NF-κB and naked keratinocyte homolog 2 (NKD2) were investigated. In vitro studies, the inhibition on inflammation and fibrogenesis by chrysophanol was further validated in TGF-β1-stimulated HK2 cells, and the regulation of chrysophanol on NKD2/NF-κB pathway was analyzed. Moreover, NKD2 was overexpressed in HK-2 cells to confirm the role of NKD2/NF-κB pathway in chrysophanol-mediated efficacy. Finally, the binding mode of chrysophanol with NKD2 was studied using in silico molecular docking and microscale thermophoresis (MST) assay. Results: Chrysophanol could significantly improve the kidney dysfunction, alleviate renal pathology, and reverse the elevated levels of renal fibrosis markers such as FN1, collagen ɑI and α-SMA. Furthermore, chrysophanol effectively inhibited TNF-α, IL-6, and IL-1β production, and suppressed NF-κB activation and NKD2 expression. The findings of in vitro study were consistent with those of animal expriment. Using NKD2-overexpressing HK-2 cells, we also demonstrated that overexpression of NKD2 significantly compromised the anti-fibrotic effects of chrysophanol. In addition, molecular docking and MST analysis revealed that NKD2 was a direct target of chrysophanol. Conclusion: Together, our work demonstrated for the first time that chrysophanol could effectively ameliorate renal fibrosis by inhibiting NKD2/NF-κB pathway. Chrysophanol can potentially prevent CKD by suppressing renal NKD2 expression directly.
MeSH term(s)	Adaptor Proteins, Signal Transducing ; Animals ; Anthraquinones ; Calcium-Binding Proteins ; Fibrosis ; Humans ; Inflammation ; Kidney ; Mice ; Molecular Docking Simulation ; NF-kappa B ; Renal Insufficiency, Chronic ; Rheum ; Transforming Growth Factor beta1 ; Ureteral Obstruction
Chemical Substances	Adaptor Proteins, Signal Transducing ; Anthraquinones ; Calcium-Binding Proteins ; NF-kappa B ; NKD2 protein, human ; Transforming Growth Factor beta1 ; chrysophanic acid (N1ST8V8RR2)
Language	English
Publishing date	2022-08-03
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2022.154381
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Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Flavonoids from Scutellaria barbata D. Don exert antitumor activity in colorectal cancer through inhibited autophagy and promoted apoptosis via ATF4/sestrin2 pathway.

Liu, Lianfang / Liu, Tianya / Tao, Weiwei / Liao, Naikai / Yan, Qiuying / Li, Liu / Tan, Jiani / Shen, Weixing / Cheng, Haibo / Sun, Dongdong

Phytomedicine : international journal of phytotherapy and phytopharmacology

2022 Volume 99, Page(s) 154007

Abstract: Purpose: Scutellaria barbata D. Don (SB), mainly containing flavonoids, has been frequently used for cancer treatment. However, little research has investigated the antitumor activity of flavonoids from SB (FSB). The current study aimed to assess the ... ...

Abstract	Purpose: Scutellaria barbata D. Don (SB), mainly containing flavonoids, has been frequently used for cancer treatment. However, little research has investigated the antitumor activity of flavonoids from SB (FSB). The current study aimed to assess the antitumor effect of TFSB and elucidate the probable underlying mechanism in vivo and in vitro. Study design: FSB was prepared, and its chemical composition was characterized by HPLC-MS. Colorectal HCT116 cells were treated with various concentration of FSB. The viability, proliferation, apoptosis, migration, and autophagy of HCT116 cells were studied, as were further confirmed in tumor xenografts. Methods: Cell viability and proliferation were respectively examined by MTT and EdU staining. ROS was determined with DCFH-DA, and cell apoptosis was detected using flow cytometry. Transwell and wound-healing assays were performed to evaluate cell migration. Immunofluorescence was employed to evaluate sestrin2 and ATF4 level. The protein expressions of p-AMPK, p-ULK1, p-mTOR, 4E-BP1, LC3-I/II, cleaved-caspase-3, Bax, and bcl-2 were investigated by western blot. ATF4 was overexpressed in experiments to explore the role of ATF4/sestrin2 pathway in FSB-mediated efficacy. Results: FSB clearly reduced the cell viability, promoted ROS generation, and induced apoptosis in HCT116 cells by down-regulated Bcl-2, and increased cleaved-caspase-3 and Bax. Furthermore, FSB significantly inhibited migration of colorectal cells in a dose-dependent manner. Further mechanistic study indicated that FSB upregulated p-mTOR protein level, and reduced p-AMPK, p-ULK1, p-mTOR, p-4E-BP1 and LC3-I/II expression, which were major autophagy-related genes. In addition, FSB could cause downregulation of endogenous mTOR inhibitor sestrin2 and ATF4 expression. Transient overexpression of ATF4 resulted in mTOR and sestrin2 inhibition, and significantly compromised the effects of FSB on apoptosis and autophagy in HCT116 cells. Conclusion: Our results reveal, for the first time, that FSB exerts antitumor activity through autophagy inhibition and apoptosis induction via ATF4/sestrin2 pathway in colorectal cancer cells. Scutellaria barbata D. Don may have great potential in the application for the prevention and treatment of human colorectal cancer.
Language	English
Publishing date	2022-02-18
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2022.154007
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Article: Flavonoids from Scutellaria barbata D. Don exert antitumor activity in colorectal cancer through inhibited autophagy and promoted apoptosis via ATF4/sestrin2 pathway

Liu, Lianfang / Liu, Tianya / Tao, Weiwei / Liao, Naikai / Yan, Qiuying / Li, Liu / Tan, Jiani / Shen, Weixing / Cheng, Haibo / Sun, Dongdong

Phytomedicine. 2022 May, v. 99

2022

Abstract: Scutellaria barbata D. Don (SB), mainly containing flavonoids, has been frequently used for cancer treatment. However, little research has investigated the antitumor activity of flavonoids from SB (FSB). The current study aimed to assess the antitumor ... ...

Abstract	Scutellaria barbata D. Don (SB), mainly containing flavonoids, has been frequently used for cancer treatment. However, little research has investigated the antitumor activity of flavonoids from SB (FSB). The current study aimed to assess the antitumor effect of TFSB and elucidate the probable underlying mechanism in vivo and in vitro. FSB was prepared, and its chemical composition was characterized by HPLC-MS. Colorectal HCT116 cells were treated with various concentration of FSB. The viability, proliferation, apoptosis, migration, and autophagy of HCT116 cells were studied, as were further confirmed in tumor xenografts. Cell viability and proliferation were respectively examined by MTT and EdU staining. ROS was determined with DCFH-DA, and cell apoptosis was detected using flow cytometry. Transwell and wound-healing assays were performed to evaluate cell migration. Immunofluorescence was employed to evaluate sestrin2 and ATF4 level. The protein expressions of p-AMPK, p-ULK1, p-mTOR, 4E-BP1, LC3-I/II, cleaved-caspase-3, Bax, and bcl-2 were investigated by western blot. ATF4 was overexpressed in experiments to explore the role of ATF4/sestrin2 pathway in FSB-mediated efficacy. FSB clearly reduced the cell viability, promoted ROS generation, and induced apoptosis in HCT116 cells by down-regulated Bcl-2, and increased cleaved-caspase-3 and Bax. Furthermore, FSB significantly inhibited migration of colorectal cells in a dose-dependent manner. Further mechanistic study indicated that FSB upregulated p-mTOR protein level, and reduced p-AMPK, p-ULK1, p-mTOR, p-4E-BP1 and LC3-I/II expression, which were major autophagy-related genes. In addition, FSB could cause downregulation of endogenous mTOR inhibitor sestrin2 and ATF4 expression. Transient overexpression of ATF4 resulted in mTOR and sestrin2 inhibition, and significantly compromised the effects of FSB on apoptosis and autophagy in HCT116 cells. Our results reveal, for the first time, that FSB exerts antitumor activity through autophagy inhibition and apoptosis induction via ATF4/sestrin2 pathway in colorectal cancer cells. Scutellaria barbata D. Don may have great potential in the application for the prevention and treatment of human colorectal cancer.
Keywords	Scutellaria barbata ; Western blotting ; antineoplastic activity ; apoptosis ; autophagy ; cancer therapy ; cell movement ; cell viability ; colorectal neoplasms ; dose response ; flavonoids ; flow cytometry ; fluorescent antibody technique ; humans ; protein content ; xenotransplantation
Language	English
Dates of publication	2022-05
Publishing place	Elsevier GmbH
Document type	Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2022.154007
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Zs.A 4115: Show issues

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Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article: Chrysophanol, a main anthraquinone from Rheum palmatum L. (rhubarb), protects against renal fibrosis by suppressing NKD2/NF-κB pathway

Gu, Mingjia / Zhou, Yufeng / Liao, Naikai / Wei, Qingxue / Bai, Zijun / Bao, Neng / Zhu, Ying / Zhang, Hang / Gao, Leiping / Cheng, Xiaolan

Phytomedicine. 2022 Oct., v. 105

2022

Abstract: Chronic kidney disease (CKD), characterized as renal dysfunction and multi-system damage, has become a serious public health problem with high prevalence and mortality. Rheum palmatum L. (rhubarb) is one of the most widely used Chinese herb with renal ... ...

Abstract	Chronic kidney disease (CKD), characterized as renal dysfunction and multi-system damage, has become a serious public health problem with high prevalence and mortality. Rheum palmatum L. (rhubarb) is one of the most widely used Chinese herb with renal protective activity. However, the active components and underlying mechanisms of rhubarb remain unknown. In this work, we tried to explore the pharmacological mechanism of chrysophanol, a main anthraquinone from rhubarb, against CKD by in vivo and in vitro models. The therapeutic effect of chrysophanol and its underlying mechanism were investigated using CKD mouse model induced by unilateral ureteral occlusion (UUO), and human kidney 2 (HK-2) cells stimulated by TGF-β1 in vivo. The impact of chrysophanol on renal function, inflammation, fibrosis of CKD mice were evaluated. Then, the protein expressions of FN1, collagen ɑI, α-SMA, NF-κB and naked keratinocyte homolog 2 (NKD2) were investigated. In vitro studies, the inhibition on inflammation and fibrogenesis by chrysophanol was further validated in TGF-β1-stimulated HK2 cells, and the regulation of chrysophanol on NKD2/NF-κB pathway was analyzed. Moreover, NKD2 was overexpressed in HK-2 cells to confirm the role of NKD2/NF-κB pathway in chrysophanol-mediated efficacy. Finally, the binding mode of chrysophanol with NKD2 was studied using in silico molecular docking and microscale thermophoresis (MST) assay. Chrysophanol could significantly improve the kidney dysfunction, alleviate renal pathology, and reverse the elevated levels of renal fibrosis markers such as FN1, collagen ɑI and α-SMA. Furthermore, chrysophanol effectively inhibited TNF-α, IL-6, and IL-1β production, and suppressed NF-κB activation and NKD2 expression. The findings of in vitro study were consistent with those of animal expriment. Using NKD2-overexpressing HK-2 cells, we also demonstrated that overexpression of NKD2 significantly compromised the anti-fibrotic effects of chrysophanol. In addition, molecular docking and MST analysis revealed that NKD2 was a direct target of chrysophanol. Together, our work demonstrated for the first time that chrysophanol could effectively ameliorate renal fibrosis by inhibiting NKD2/NF-κB pathway. Chrysophanol can potentially prevent CKD by suppressing renal NKD2 expression directly.
Keywords	Rheum palmatum ; anthraquinones ; collagen ; computer simulation ; fibrosis ; humans ; inflammation ; interleukin-6 ; keratinocytes ; kidney diseases ; kidneys ; mice ; mortality ; public health ; renal function ; rhubarb ; therapeutics
Language	English
Dates of publication	2022-10
Publishing place	Elsevier GmbH
Document type	Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2022.154381
Database	NAL-Catalogue (AGRICOLA)

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Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Establishment of a risk score model for bladder urothelial carcinoma based on energy metabolism‐related genes and their relationships with immune infiltration

Huang, Caihong / Li, Yexin / Ling, Qiang / Wei, Chunmeng / Fang, Bo / Mao, Xingning / Yang, Rirong / Zhang, Lulu / Huang, Shengzhu / Cheng, Jiwen / Liao, Naikai / Wang, Fubo / Mo, Linjian / Mo, Zengnan / Li, Longman

FEBS Open Bio. 2023 Apr., v. 13, no. 4, p. 736-750

2023 , Page(s) 736–750

Abstract: Bladder urothelial carcinoma (BLCA) is a common malignant tumor of the human urinary system, and a large proportion of BLCA patients have a poor prognosis. Therefore, there is an urgent need to find more efficient and sensitive biomarkers for the ... ...

Abstract	Bladder urothelial carcinoma (BLCA) is a common malignant tumor of the human urinary system, and a large proportion of BLCA patients have a poor prognosis. Therefore, there is an urgent need to find more efficient and sensitive biomarkers for the prognosis of BLCA patients in clinical practice. RNA sequencing (RNA‐seq) data and clinical information were obtained from The Cancer Genome Atlas, and 584 energy metabolism‐related genes (EMRGs) were obtained from the Reactome pathway database. Cox regression analysis and least absolute shrinkage and selection operator analysis were applied to assess prognostic genes and build a risk score model. The estimate and cibersort algorithms were used to explore the immune microenvironment, immune infiltration, and checkpoints in BLCA patients. Furthermore, we used the Human Protein Atlas database and our single‐cell RNA‐seq datasets of BLCA patients to verify the expression of 13 EMRGs at the protein and single‐cell levels. We constructed a risk score model; the area under the curve of the model at 5 years was 0.792. The risk score was significantly correlated with the immune markers M0 macrophages, M2 macrophages, CD8 T cells, follicular helper T cells, regulatory T cells, and dendritic activating cells. Furthermore, eight immune checkpoint genes were significantly upregulated in the high‐risk group. The risk score model can accurately predict the prognosis of BLCA patients and has clinical application value. In addition, according to the differences in immune infiltration and checkpoints, BLCA patients with the most significant benefit can be selected for immune checkpoint inhibitor therapy.
Keywords	RNA ; biomarkers ; bladder ; carcinoma ; data collection ; databases ; energy ; genome ; humans ; macrophages ; models ; prognosis ; regression analysis ; risk ; sequence analysis ; therapeutics
Language	English
Dates of publication	2023-04
Size	p. 736-750
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	2651702-4
ISSN	2211-5463
ISSN	2211-5463
DOI	10.1002/2211-5463.13580
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Establishment of a risk score model for bladder urothelial carcinoma based on energy metabolism-related genes and their relationships with immune infiltration.

Huang, Caihong / Li, Yexin / Ling, Qiang / Wei, Chunmeng / Fang, Bo / Mao, Xingning / Yang, Rirong / Zhang, LuLu / Huang, Shengzhu / Cheng, Jiwen / Liao, Naikai / Wang, Fubo / Mo, Linjian / Mo, Zengnan / Li, Longman

FEBS open bio

2023 Volume 13, Issue 4, Page(s) 736–750

Abstract	Bladder urothelial carcinoma (BLCA) is a common malignant tumor of the human urinary system, and a large proportion of BLCA patients have a poor prognosis. Therefore, there is an urgent need to find more efficient and sensitive biomarkers for the prognosis of BLCA patients in clinical practice. RNA sequencing (RNA-seq) data and clinical information were obtained from The Cancer Genome Atlas, and 584 energy metabolism-related genes (EMRGs) were obtained from the Reactome pathway database. Cox regression analysis and least absolute shrinkage and selection operator analysis were applied to assess prognostic genes and build a risk score model. The estimate and cibersort algorithms were used to explore the immune microenvironment, immune infiltration, and checkpoints in BLCA patients. Furthermore, we used the Human Protein Atlas database and our single-cell RNA-seq datasets of BLCA patients to verify the expression of 13 EMRGs at the protein and single-cell levels. We constructed a risk score model; the area under the curve of the model at 5 years was 0.792. The risk score was significantly correlated with the immune markers M0 macrophages, M2 macrophages, CD8 T cells, follicular helper T cells, regulatory T cells, and dendritic activating cells. Furthermore, eight immune checkpoint genes were significantly upregulated in the high-risk group. The risk score model can accurately predict the prognosis of BLCA patients and has clinical application value. In addition, according to the differences in immune infiltration and checkpoints, BLCA patients with the most significant benefit can be selected for immune checkpoint inhibitor therapy.
MeSH term(s)	Humans ; Carcinoma, Transitional Cell ; Urinary Bladder Neoplasms/genetics ; Urinary Bladder ; Energy Metabolism/genetics ; Algorithms ; Tumor Microenvironment/genetics
Language	English
Publishing date	2023-03-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2651702-4
ISSN	2211-5463 ; 2211-5463
ISSN (online)	2211-5463
ISSN	2211-5463
DOI	10.1002/2211-5463.13580
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Identification of tumor immunophenotypes associated with immunotherapy response in bladder cancer.

Liang, Hai-Qi / Liao, Nai-Kai / Yang, Shu-Bo / Wei, Qiu-Ju / Tan, Shu-Ting / Zhai, Gao-Qiang / Lu, Jiang-Ting / Huang, Yi-Cheng / Deng, Xiao-Bin / Mo, Lin-Jian / Cheng, Ji-Wen

International journal of urology : official journal of the Japanese Urological Association

2023 Volume 30, Issue 12, Page(s) 1122–1132

Abstract: Objectives: This study aims to reveal immunophenotypes associated with immunotherapy response in bladder cancer, identify the signature genes of immune subtypes, and provide new molecular targets for improving immunotherapy response.: Methods: ... ...

Abstract	Objectives: This study aims to reveal immunophenotypes associated with immunotherapy response in bladder cancer, identify the signature genes of immune subtypes, and provide new molecular targets for improving immunotherapy response. Methods: Bladder cancer immunophenotypes were characterized in the bulk RNA sequencing dataset GSE32894 and Imvigor210, and gene expression signatures were established to identify the immunophenotypes. Expression of gene signatures were validated in single-cell RNA sequencing dataset GSE145140 and human proteins expression data source. Investigation of Immunotherapy Response was performed in IMvigor210 dataset. Prognosis of tumor immunophenotypes was further analyzed. Results: Inflamed and immune-excluded immunophenotypes were characterized based on the tumor immune cell scores. Risk score models that were established rely on RNA sequencing profiles and overall survival of bladder cancer cohorts. The inflamed tumors had lower risk scores, and the low-risk tumors were more likely to respond to atezolizumab, receiving complete response/partial response (CR/PR). Patients who responded to atezolizumab had higher SRRM4 and lower NPHS1 and TMEM72 expression than the non-responders. SRRM4 expression was a protective factor for bladder cancer prognosis, while the NPHS1 and TMEM72 showed the opposite pattern. Conclusion: This study provided a novel classification method for tumor immunophenotypes. Bladder cancer immunophenotypes can predict the response to immune checkpoint blockade. The immunophenotypes can be identified by the expression of signature genes.
MeSH term(s)	Humans ; Urinary Bladder Neoplasms/drug therapy ; Urinary Bladder Neoplasms/genetics ; Urinary Bladder ; Immunotherapy ; Nephrotic Syndrome ; Tumor Microenvironment ; Prognosis ; Nerve Tissue Proteins
Chemical Substances	SRRM4 protein, human ; Nerve Tissue Proteins
Language	English
Publishing date	2023-08-21
Publishing country	Australia
Document type	Journal Article
ZDB-ID	1328401-0
ISSN	1442-2042 ; 0919-8172
ISSN (online)	1442-2042
ISSN	0919-8172
DOI	10.1111/iju.15276
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 4460: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 278: Show issues

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Article ; Online: A study comparing plasmakinetic enucleation with bipolar plasmakinetic resection of the prostate for benign prostatic hyperplasia.

Liao, Naikai / Yu, JianJun

Journal of endourology

2012 Volume 26, Issue 7, Page(s) 884–888

Abstract: Background and purpose: Plasmakinetic enucleation of the prostate (PKEP) has been reported to be a new method for the transurethral management of benign prostatic hyperplasia (BPH). Our objective was to compare the safety and efficiency of PKEP with ... ...

Abstract	Background and purpose: Plasmakinetic enucleation of the prostate (PKEP) has been reported to be a new method for the transurethral management of benign prostatic hyperplasia (BPH). Our objective was to compare the safety and efficiency of PKEP with that of bipolar plasmakinetic resection of the prostate (PKRP) in the management of BPH. Patients and methods: A total of 303 patients with lower urinary tract symptoms associated with BPH were included in our study. Of these, 143 patients underwent PKRP and 160 underwent PKEP. All patients were preoperatively assessed and evaluated at 3 months after surgery. International Prostate Symptom Score (IPSS), quality of life (QoL), postvoid residual urine (PVRU) volume, and maximum flow rate (Qmax) were obtained at the follow-up. The perioperative data and postoperative outcomes were compared. Immediate and late complications were recorded. Results: There were no significant differences between the two surgical groups preoperatively. The mean prostate volumes in the PKRP and PKEP groups were 75.6 cc and 77.3 cc, respectively. Both groups were similar with respect to operative time, resected tissue weight, catheterization time, and the total length of hospital stay. The blood loss observed in the PKEP group was significantly less than that of conventional PKRP (P<0.0001). PKEP was significantly superior to PKRP in terms of the transient incontinence in the postoperative period (P=0.03). Both groups resulted in a significant improvement from baseline in terms of IPSS, QoL, Qmax, and PVRU volume values. No significant difference was found between them, however. Conclusions: PKEP is a safe and effective method for the transurethral management of BPH. Compared with PKRP, the main advantage of PKEP is the decreased risk of blood loss and transient incontinence.
MeSH term(s)	Electrodes ; Electrosurgery/adverse effects ; Electrosurgery/methods ; Follow-Up Studies ; Humans ; Male ; Perioperative Care ; Plasma Gases/therapeutic use ; Postoperative Complications/etiology ; Prostate/surgery ; Prostatic Hyperplasia/physiopathology ; Prostatic Hyperplasia/surgery
Chemical Substances	Plasma Gases
Language	English
Publishing date	2012-07
Publishing country	United States
Document type	Comparative Study ; Journal Article
ZDB-ID	356931-7
ISSN	1557-900X ; 0892-7790
ISSN (online)	1557-900X
ISSN	0892-7790
DOI	10.1089/end.2011.0358
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Zs.A 2628: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 208: Show issues

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Article ; Online: Transurethral bipolar plasmakinetic resection combined with 2 μm continuous wave laser vaporization: a new method for the treatment of large volume benign prostatic hyperplasia.

Liao, Naikai / Yu, JianJun

Photomedicine and laser surgery

2012 Volume 30, Issue 6, Page(s) 320–324

Abstract: Objective: The aim of this study was to evaluate the safety and efficiency of transurethral bipolar plasmakinetic resection of the prostate (PKRP) combined with 2 μm laser vaporization in the management of large prostates (>80 mL).: Background data: ... ...

Abstract	Objective: The aim of this study was to evaluate the safety and efficiency of transurethral bipolar plasmakinetic resection of the prostate (PKRP) combined with 2 μm laser vaporization in the management of large prostates (>80 mL). Background data: The safety and efficiency of transurethral vaporesection of the prostate with benign prostatic hyperplasia (BPH), using a 2 μm laser system, have been verified. However, this method does still not manage large volume prostates efficiently. Methods: From October 2009 to June 2010, 120 BPH patients with a median prostatic volume of 106.7 (±16.7) mL (range, 82.5-156.8 mL) were randomized for surgical treatment with PKRP combined with 2 μm laser vaporization (n=58) or PKRP only (n=62). All patients were preoperatively assessed with subjective symptoms score. Preoperative and perioperative parameters at 3-, 6-, and 9-month follow-up were also evaluated. All complications were recorded. Results: PKRP combined with 2 μm laser vaporization was significantly superior to PKRP alone in terms of operative time, irrigation time, catheterization time, hospital stay, and hemoglobin decrease. The blood transfusion and urinary tract infection observed in the PKRP combined with 2 μm laser vaporization group was significantly less than that of the groups that received PKRP only. Both groups were similar with respect to resected tissue weight, transient incontinence, urethral stricture and retrograde ejaculation in the postoperative period. Both groups showed a significant improvement from baseline in terms of International Prostate Symptom Score (IPSS), quality of life (QOL), maximum urinary flow rate (Qmax), and pulmonary vascular resistance unit (PVRU) values. However, no significant difference was found between them. Conclusions: PKRP combined with 2 μm laser vaporization, which combines the advantages of both PKRP and 2 μm laser, is superior for its shorter operation time, less bleeding, and better efficiency. It may be a safer and more effective method for the treatment of BPH in large prostates.
MeSH term(s)	Aged ; Aged, 80 and over ; Combined Modality Therapy ; Follow-Up Studies ; Humans ; Laser Therapy/adverse effects ; Laser Therapy/methods ; Lasers ; Length of Stay ; Male ; Middle Aged ; Minimally Invasive Surgical Procedures/methods ; Organ Size ; Pain Measurement ; Pain, Postoperative/physiopathology ; Postoperative Complications/physiopathology ; Postoperative Complications/therapy ; Prostatic Hyperplasia/diagnostic imaging ; Prostatic Hyperplasia/pathology ; Prostatic Hyperplasia/surgery ; Reference Values ; Risk Assessment ; Safety Management ; Severity of Illness Index ; Transurethral Resection of Prostate/adverse effects ; Transurethral Resection of Prostate/methods ; Treatment Outcome ; Ultrasonography ; Volatilization
Language	English
Publishing date	2012-06
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Randomized Controlled Trial
ZDB-ID	2158446-1
ISSN	1557-8550 ; 1549-5418
ISSN (online)	1557-8550
ISSN	1549-5418
DOI	10.1089/pho.2011.3181
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Zs.A 1908: Show issues

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Article ; Online: A study comparing dusting to basketing for renal stones ≤ 2 cm during flexible ureteroscopy.

Liao, NaiKai / Tan, ShuTing / Yang, ShuBo / Zhai, GaoQiang / Li, ChengYang / Li, TianYu / Chen, Yang / Mo, LinJian / Cheng, JiWen

International braz j urol : official journal of the Brazilian Society of Urology

2022 Volume 49, Issue 2, Page(s) 194–201

Abstract: Objectives: To compare the dusting efficiency and safety with basketing for treating renal stones ≤ 2 cm during flexible ureteroscopy (fURS).: Materials and methods: This study included 218 patients with renal stones ≤ 2 cm treated with fURS. Among ... ...

Abstract	Objectives: To compare the dusting efficiency and safety with basketing for treating renal stones ≤ 2 cm during flexible ureteroscopy (fURS). Materials and methods: This study included 218 patients with renal stones ≤ 2 cm treated with fURS. Among them, 106 patients underwent dusting, and 112 patients underwent fragmentation with basket extraction. All patients were followed up for 3 months postoperatively. The operating time, lasing time, stone-free rate (SFR) and complication rate were compared. Results: The mean stone size in the dusting group was 1.3 cm, whereas 1.4 cm in the basketing group. The mean operative time was significantly lower in the dusting group than in the basketing group (43.1±11.7 minutes VS 60.5±13.4 minutes, P < 0.05), but the lasing time was significantly longer for the dusting group than for the basketing group (17.7±3.9 minutes VS 14.1±3.6 minutes, P < 0.05). SFR was signiﬁcantly higher in the basketing group immediately after the operation and follow-up after 1 month (76.8% vs 55.7%, P= 0.001 and 88.4% vs 78.3%, P = 0.045). However, the SFR was similar for both groups (88.8% in the dusting group vs. 90.2% in the basketing group) after 3 months postoperatively. There was no statistical difference in the complication rates between the two groups. Conclusions: Dusting has advantages in shortening the operation time and reducing the operation cost, but the lasing time was longer compared with the basketing. Although there is no difference in long-term effect, basketing is superior to dusting in terms of short-term SFR. Moreover, dusting should be avoided in some special cases and basketing a better choice. Both techniques are effective for the treatment of renal stones ≤ 2 cm and choice depends on patient demographic and stone characteristics.
MeSH term(s)	Humans ; Ureteroscopy/methods ; Lithotripsy, Laser/methods ; Kidney Calculi/surgery ; Ureteroscopes ; Operative Time ; Treatment Outcome
Language	English
Publishing date	2022-12-17
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2206649-4
ISSN	1677-6119 ; 1677-5538
ISSN (online)	1677-6119
ISSN	1677-5538
DOI	10.1590/S1677-5538.IBJU.2022.0382
Database	MEDical Literature Analysis and Retrieval System OnLINE

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