Article ; Online: Allograft Model of Aortic Arch Segment Grafting to Abdominal Aorta Through End-to-Side Anastomosis in Mice.
Journal of cardiovascular translational research
2024
Abstract: The mouse aortic transplantation model is a valuable tool for investigating the mechanisms of atherosclerosis regression, but few laboratories can generate it due to the operation difficulty, especially for the style of end-to-side anastomosis, which ... ...
Abstract | The mouse aortic transplantation model is a valuable tool for investigating the mechanisms of atherosclerosis regression, but few laboratories can generate it due to the operation difficulty, especially for the style of end-to-side anastomosis, which facilitates syngeneic heterotopic transplanting a plaque-rich aortic arch into the abdominal aorta. Here we provide a modified protocol for generating this allograft model, which is capable of overcoming several critical surgical challenges such as separating a longer abdominal aorta segment, reducing bleeding and thrombosis, optimizing aortotomy, and improving end-to-side anastomosis to guarantee a potent graft. By transplanting plaque-rich aortic arches into the abdominal aorta of wildtype mice, a high operation success rate (over 90%) was noted with aortic clamping time under 60 min, the graft potency was satisfactory evidenced by examinations of micro-CT, ultrasound, and lower limb blood flow measurement, while a significant atherosclerosis regression was observed in the grafts at 1 week after transplantation. |
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Language | English |
Publishing date | 2024-02-26 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 2422411-X |
ISSN | 1937-5395 ; 1937-5387 |
ISSN (online) | 1937-5395 |
ISSN | 1937-5387 |
DOI | 10.1007/s12265-024-10495-w |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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