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  1. AU="Lieve E. H. van der Donk"
  2. AU="Großer, Matthias"
  3. AU="Ong, Edison"
  4. AU=Lavery James V
  5. AU=Moss Arthur J
  6. AU="Ni, Dongchun"
  7. AU="Yang, Yanfan"
  8. AU="Shona Manning"
  9. AU=Charters Pia F P AU=Charters Pia F P
  10. AU="Adumuah, Naa N"
  11. AU="Rodrigues, Jonathan Carl Luis"
  12. AU=Seidel Bastian M
  13. AU="Duan Weimin"
  14. AU=Ioanas M
  15. AU="Nancy Zambon"
  16. AU="Kumawat, Sunita"
  17. AU=Bogliacino Francesco
  18. AU="Setter, Peter"
  19. AU=Shikata Chihiro
  20. AU="Jordan P. Metcalf"
  21. AU=Peri?i? Nanut Milica AU=Peri?i? Nanut Milica
  22. AU="Pramod, Ganapathiraju"
  23. AU="Fu, Chu-Jun"
  24. AU="Nejad, Harry G."
  25. AU="Zhang, Q E"
  26. AU="Oppenheim, Madeline"

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  1. Artikel ; Online: SARS-CoV-2 suppresses TLR4-induced immunity by dendritic cells via C-type lectin receptor DC-SIGN.

    Lieve E H van der Donk / Marta Bermejo-Jambrina / John L van Hamme / Mette M W Volkers / Ad C van Nuenen / Neeltje A Kootstra / Teunis B H Geijtenbeek

    PLoS Pathogens, Vol 19, Iss 10, p e

    2023  Band 1011735

    Abstract: SARS-CoV-2 causes COVID-19, an infectious disease with symptoms ranging from a mild cold to severe pneumonia, inflammation, and even death. Although strong inflammatory responses are a major factor in causing morbidity and mortality, superinfections with ...

    Abstract SARS-CoV-2 causes COVID-19, an infectious disease with symptoms ranging from a mild cold to severe pneumonia, inflammation, and even death. Although strong inflammatory responses are a major factor in causing morbidity and mortality, superinfections with bacteria during severe COVID-19 often cause pneumonia, bacteremia and sepsis. Aberrant immune responses might underlie increased sensitivity to bacteria during COVID-19 but the mechanisms remain unclear. Here we investigated whether SARS-CoV-2 directly suppresses immune responses to bacteria. We studied the functionality of human dendritic cells (DCs) towards a variety of bacterial triggers after exposure to SARS-CoV-2 Spike (S) protein and SARS-CoV-2 primary isolate (hCoV-19/Italy). Notably, pre-exposure of DCs to either SARS-CoV-2 S protein or a SARS-CoV-2 isolate led to reduced type I interferon (IFN) and cytokine responses in response to Toll-like receptor (TLR)4 agonist lipopolysaccharide (LPS), whereas other TLR agonists were not affected. SARS-CoV-2 S protein interacted with the C-type lectin receptor DC-SIGN and, notably, blocking DC-SIGN with antibodies restored type I IFN and cytokine responses to LPS. Moreover, blocking the kinase Raf-1 by a small molecule inhibitor restored immune responses to LPS. These results suggest that SARS-CoV-2 modulates DC function upon TLR4 triggering via DC-SIGN-induced Raf-1 pathway. These data imply that SARS-CoV-2 actively suppresses DC function via DC-SIGN, which might account for the higher mortality rates observed in patients with COVID-19 and bacterial superinfections.
    Schlagwörter Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 572 ; 616
    Sprache Englisch
    Erscheinungsdatum 2023-10-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: An optimized retroviral toolbox for overexpression and genetic perturbation of primary lymphocytes

    Lieve E. H. van der Donk / Jet van der Spek / Tom van Duivenvoorde / Marieke S. ten Brink / Teunis B. H. Geijtenbeek / Coenraad P. Kuijl / Jeroen W. J. van Heijst / Louis S. Ates

    Biology Open, Vol 11, Iss

    2022  Band 2

    Abstract: Genetic manipulation of primary lymphocytes is crucial for both clinical purposes and fundamental research. Despite their broad use, we encountered a paucity of data on systematic comparison and optimization of retroviral vectors, the workhorses of ... ...

    Abstract Genetic manipulation of primary lymphocytes is crucial for both clinical purposes and fundamental research. Despite their broad use, we encountered a paucity of data on systematic comparison and optimization of retroviral vectors, the workhorses of genetic modification of primary lymphocytes. Here, we report the construction and validation of a versatile range of retroviral expression vectors. These vectors can be used for the knockdown or overexpression of genes of interest in primary human and murine lymphocytes, in combination with a wide choice of selection and reporter strategies. By streamlining the vector backbone and insert design, these publicly available vectors allow easy interchangeability of the independent building blocks, such as different promoters, fluorescent proteins, surface markers and antibiotic resistance cassettes. We validated these vectors and tested the optimal promoters for in vitro and in vivo overexpression and knockdown of the murine T cell antigen receptor. By publicly sharing these vectors and the data on their optimization, we aim to facilitate genetic modification of primary lymphocytes for researchers entering this field.
    Schlagwörter retrovirus ; t cell ; lymphocytes ; overexpression ; Science ; Q ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 006
    Sprache Englisch
    Erscheinungsdatum 2022-02-01T00:00:00Z
    Verlag The Company of Biologists
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

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    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

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