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  1. Article ; Online: Comprehensive characterization of fetal and mature retinal cell identity to assess the fidelity of retinal organoids.

    Kim, Hani Jieun / O'Hara-Wright, Michelle / Kim, Daniel / Loi, To Ha / Lim, Benjamin Y / Jamieson, Robyn V / Gonzalez-Cordero, Anai / Yang, Pengyi

    Stem cell reports

    2023  Volume 18, Issue 1, Page(s) 175–189

    Abstract: Characterizing cell identity in complex tissues such as the human retina is essential for studying its development and disease. While retinal organoids derived from pluripotent stem cells have been widely used to model development and disease of the ... ...

    Abstract Characterizing cell identity in complex tissues such as the human retina is essential for studying its development and disease. While retinal organoids derived from pluripotent stem cells have been widely used to model development and disease of the human retina, there is a lack of studies that have systematically evaluated the molecular and cellular fidelity of the organoids derived from various culture protocols in recapitulating their in vivo counterpart. To this end, we performed an extensive meta-atlas characterization of cellular identities of the human eye, covering a wide range of developmental stages. The resulting map uncovered previously unknown biomarkers of major retinal cell types and those associated with cell-type-specific maturation. Using our retinal-cell-identity map from the fetal and adult tissues, we systematically assessed the fidelity of the retinal organoids in mimicking the human eye, enabling us to comprehensively benchmark the current protocols for retinal organoid generation.
    MeSH term(s) Adult ; Humans ; Retina/metabolism ; Pluripotent Stem Cells/metabolism ; Neurons ; Organoids ; Cell Differentiation ; Induced Pluripotent Stem Cells/metabolism
    Language English
    Publishing date 2023-01-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2720528-9
    ISSN 2213-6711 ; 2213-6711
    ISSN (online) 2213-6711
    ISSN 2213-6711
    DOI 10.1016/j.stemcr.2022.12.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Differentiation of brain and retinal organoids from confluent cultures of pluripotent stem cells connected by nerve-like axonal projections of optic origin.

    Fernando, Milan / Lee, Scott / Wark, Jesse R / Xiao, Di / Lim, Benjamin Y / O'Hara-Wright, Michelle / Kim, Hani J / Smith, Grady C / Wong, Ted / Teber, Erdahl T / Ali, Robin R / Yang, Pengyi / Graham, Mark E / Gonzalez-Cordero, Anai

    Stem cell reports

    2022  Volume 17, Issue 6, Page(s) 1476–1492

    Abstract: Advances in the study of neurological conditions have been possible because of pluripotent stem cell technologies and organoids. Studies have described the generation of neural ectoderm-derived retinal and brain structures from pluripotent stem cells. ... ...

    Abstract Advances in the study of neurological conditions have been possible because of pluripotent stem cell technologies and organoids. Studies have described the generation of neural ectoderm-derived retinal and brain structures from pluripotent stem cells. However, the field is still troubled by technical challenges, including high culture costs and variability. Here, we describe a simple and economical protocol that reproducibly gives rise to the neural retina and cortical brain regions from confluent cultures of stem cells. The spontaneously generated cortical organoids are transcriptionally comparable with organoids generated by other methods. Furthermore, these organoids showed spontaneous functional network activity and proteomic analysis confirmed organoids maturity. The generation of retinal and brain organoids in close proximity enabled their mutual isolation. Suspension culture of this complex organoid system demonstrated the formation of nerve-like structures connecting retinal and brain organoids, which might facilitate the investigation of neurological diseases of the eye and brain.
    MeSH term(s) Brain ; Cell Differentiation ; Induced Pluripotent Stem Cells ; Organoids ; Pluripotent Stem Cells ; Proteomics ; Retina
    Language English
    Publishing date 2022-05-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2720528-9
    ISSN 2213-6711 ; 2213-6711
    ISSN (online) 2213-6711
    ISSN 2213-6711
    DOI 10.1016/j.stemcr.2022.04.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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