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Article: Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?

Lima, Monique da Rocha Queiroz / de Lima, Raquel Curtinhas / de Azeredo, Elzinandes Leal / Dos Santos, Flavia Barreto

2021 Volume 11, Issue 5

Abstract: In Brazil, chikungunya emerged in 2014, and by 2016, co-circulated with other arbovirosis, such as dengue and zika. ELISAs (Enzyme-Linked Immunosorbent Assays) are the most widely used approach for arboviruses diagnosis. However, some limitations include ...

Abstract	In Brazil, chikungunya emerged in 2014, and by 2016, co-circulated with other arbovirosis, such as dengue and zika. ELISAs (Enzyme-Linked Immunosorbent Assays) are the most widely used approach for arboviruses diagnosis. However, some limitations include antibody cross reactivities when viruses belong to the same genus, and sensitivity variations in distinct epidemiological scenarios. As chikungunya virus (CHIKV) is an alphavirus, no serological cross reactivity with dengue virus (DENV) should be observed. Here, we evaluated a routinely used chikungunya commercial IgM (Immunoglobulin M) ELISA test (Anti-Chikungunya IgM ELISA, Euroimmun) to assess its performance in confirming chikungunya in a dengue endemic area. Samples (
Language	English
Publishing date	2021-04-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662336-5
ISSN	2075-4418
ISSN	2075-4418
DOI	10.3390/diagnostics11050819
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: A Chikungunya Virus Multiepitope Recombinant Protein Expressed from the Binary System Insect Cell/Recombinant Baculovirus Is Useful for Laboratorial Diagnosis of Chikungunya

Silva, Leonardo Assis da / Lima, Monique da Rocha Queiroz / de Camargo, Brenda Rabello / Guimarães, Dyeferson Kened da Silva Coelho / Barbastefano, Anabele Azevedo Lima / Lima, Raquel Curtinhas de / Damasco, Paulo Vieira / Cunha, Rivaldo Venâncio da / de Souza, Luiz José / de Azeredo, Elzinandes Leal / de-Oliveira-Pinto, Luzia Maria / Nagata, Tatsuya / Ardisson-Araújo, Daniel M. P. / dos Santos, Flavia Barreto / Morais Ribeiro, Bergmann

Microorganisms. 2022 July 18, v. 10, no. 7

2022

Abstract: Chikungunya virus (CHIKV) is an arbovirus currently distributed worldwide, causing a disease that shares clinical signs and symptoms with other illnesses, such as dengue and Zika and leading to a challenging clinical differential diagnosis. In Brazil, ... ...

Abstract	Chikungunya virus (CHIKV) is an arbovirus currently distributed worldwide, causing a disease that shares clinical signs and symptoms with other illnesses, such as dengue and Zika and leading to a challenging clinical differential diagnosis. In Brazil, CHIKV emerged in 2014 with the simultaneous introduction of both Asian and East/Central/South African (ECSA) genotypes. Laboratorial diagnosis of CHIKV is mainly performed by molecular and serological assays, with the latter more widely used. Although many commercial kits are available, their costs are still high for many underdeveloped and developing countries where the virus circulates. Here we described the development and evaluation of a multi-epitope recombinant protein-based IgG-ELISA (MULTREC IgG-ELISA) test for the specific detection of anti-CHIKV antibodies in clinical samples, as an alternative approach for laboratorial diagnosis. The MULTREC IgG-ELISA showed 86.36% of sensitivity and 100% of specificity, and no cross-reactivity with other exanthematic diseases was observed. The recombinant protein was expressed from the binary system insect cell/baculovirus using the crystal-forming baculoviral protein polyhedrin as a carrier of the target recombinant protein to facilitate recovery. The crystals were at least 10 times smaller in size and had an amorphous shape when compared to the polyhedrin wild-type crystal. The assay uses a multi-epitope antigen, representing two replicates of 18 amino acid sequences from the E2 region and a sequence of 17 amino acids from the nsP3 region of CHIKV. The recombinant protein was highly expressed, easy to purify and has demonstrated its usefulness in confirming chikungunya exposure, indeed showing a good potential tool for epidemiological surveillance.
Keywords	Baculoviridae ; Chikungunya virus ; antigens ; arboviruses ; cross reaction ; dengue ; insects ; monitoring ; recombinant proteins ; Brazil
Language	English
Dates of publication	2022-0718
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms10071451
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Diagnostic parameters and reliability of four rapid immunochromatographic tests for dengue 4.

Mata, Veronica Elizabeth / Passos, Sonia Regina Lambert / Santos, Maria Angelica Borges Dos / Buonora, Sibelle Nogueira / de Andrade, Carlos Augusto Ferreira / Lima, Monique da Rocha Queiroz / Costa, Betina Moreira / Hökerberg, Yara Hahr Marques

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases

2020 Volume 24, Issue 1, Page(s) 58–64

Abstract: Background: Although performance of rapid immunochromatographic tests (RITs) for dengue virus (DENV) serotypes 1, 2 and 3 is relatively settled, evidence on accuracy of RITs for DENV-4 are based on studies with small sample sizes and with discrepant ... ...

Abstract	Background: Although performance of rapid immunochromatographic tests (RITs) for dengue virus (DENV) serotypes 1, 2 and 3 is relatively settled, evidence on accuracy of RITs for DENV-4 are based on studies with small sample sizes and with discrepant results. Objectives: To assess accuracy and inter-observer agreement of RITs targeting dengue nonstructural protein-1 (NS1) antigen - Dengue NS1-Bioeasy™, Dengue NS1 Ag Strip-Bio-Rad™, IVB Dengue Ag NS1-Orangelife™ and Dengue NS1-K130-Bioclin™ in DENV-4 samples. Methods: Study sample (n = 324) included adults presenting at an emergency unit in Rio de Janeiro, Brazil, with fever of ≤72 h and two or more dengue symptoms. A serum sample from each patient was tested by each RIT. A positive reverse-transcription polymerase chain reaction was considered as the reference standard for dengue diagnosis. The diagnostic parameters analyzed for each RIT were sensitivity, specificity, positive and negative predictive values, and likelihood ratios. Each RIT was read by homogeneous (two junior nurses) or heterogeneous (one junior nurse and one senior biologist) pairs. Agreement was estimated by simple kappa with 95% confidence interval, positive (Ppos) and negative (Pneg) proportion concordance and prevalence and bias adjusted kappa, rated from poor (k < 0.0) to almost perfect (0.8 < k < 1.0), and perfect (k = 1). Results: NS1 RITs for DENV-4 diagnosis showed high specificity (95.9%-99.4%), but low sensitivity (14.7%-45.4%). Bioeasy™ had the best performance, with a positive likelihood ratio of 26.0 (95% CI: 8.4;81.0). Inter-observer agreement was almost perfect for all evaluated RITs. Mismatches in confirmed dengue were more common for the Bioclin™ (Ppos 88.3-90.0 %) and Orangelife™ (Ppos 91.7-94.1 %) tests. Conclusions: For DENV-4, the tested RITs had high specificity, but lower sensitivity compared to published results for other serotypes. They should not be used for screening purposes. Different brands may have very different performances. This should be considered upon deciding of using RITs in DENV-4 outbreaks.
MeSH term(s)	Adult ; Brazil ; Chromatography, Affinity/methods ; Chromatography, Affinity/standards ; Cross-Sectional Studies ; Dengue/diagnosis ; Dengue/immunology ; Dengue/virology ; Dengue Virus/isolation & purification ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Male ; Middle Aged ; Observer Variation ; Prospective Studies ; Reference Standards ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction ; Sensitivity and Specificity ; Serogroup
Language	English
Publishing date	2020-01-16
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2041400-6
ISSN	1678-4391 ; 1413-8670
ISSN (online)	1678-4391
ISSN	1413-8670
DOI	10.1016/j.bjid.2019.12.004
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Article: A Chikungunya Virus Multiepitope Recombinant Protein Expressed from the Binary System Insect Cell/Recombinant Baculovirus Is Useful for Laboratorial Diagnosis of Chikungunya.

Silva, Leonardo Assis da / Lima, Monique da Rocha Queiroz / de Camargo, Brenda Rabello / Guimarães, Dyeferson Kened da Silva Coelho / Barbastefano, Anabele Azevedo Lima / Lima, Raquel Curtinhas de / Damasco, Paulo Vieira / Cunha, Rivaldo Venâncio da / de Souza, Luiz José / de Azeredo, Elzinandes Leal / de-Oliveira-Pinto, Luzia Maria / Nagata, Tatsuya / Ardisson-Araújo, Daniel M P / Dos Santos, Flavia Barreto / Morais Ribeiro, Bergmann

Microorganisms

2022 Volume 10, Issue 7

Abstract	Chikungunya virus (CHIKV) is an arbovirus currently distributed worldwide, causing a disease that shares clinical signs and symptoms with other illnesses, such as dengue and Zika and leading to a challenging clinical differential diagnosis. In Brazil, CHIKV emerged in 2014 with the simultaneous introduction of both Asian and East/Central/South African (ECSA) genotypes. Laboratorial diagnosis of CHIKV is mainly performed by molecular and serological assays, with the latter more widely used. Although many commercial kits are available, their costs are still high for many underdeveloped and developing countries where the virus circulates. Here we described the development and evaluation of a multi-epitope recombinant protein-based IgG-ELISA (MULTREC IgG-ELISA) test for the specific detection of anti-CHIKV antibodies in clinical samples, as an alternative approach for laboratorial diagnosis. The MULTREC IgG-ELISA showed 86.36% of sensitivity and 100% of specificity, and no cross-reactivity with other exanthematic diseases was observed. The recombinant protein was expressed from the binary system insect cell/baculovirus using the crystal-forming baculoviral protein polyhedrin as a carrier of the target recombinant protein to facilitate recovery. The crystals were at least 10 times smaller in size and had an amorphous shape when compared to the polyhedrin wild-type crystal. The assay uses a multi-epitope antigen, representing two replicates of 18 amino acid sequences from the E2 region and a sequence of 17 amino acids from the nsP3 region of CHIKV. The recombinant protein was highly expressed, easy to purify and has demonstrated its usefulness in confirming chikungunya exposure, indeed showing a good potential tool for epidemiological surveillance.
Language	English
Publishing date	2022-07-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms10071451
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Article ; Online: Clinical, Virological, and Immunological Profiles of DENV, ZIKV, and/or CHIKV-Infected Brazilian Patients.

Sánchez-Arcila, Juan Camilo / Badolato-Correa, Jessica / de Souza, Thiara Manuele Alves / Paiva, Iury Amâncio / Barbosa, Luciana Santos / Nunes, Priscila Conrado Guerra / Lima, Monique da Rocha Queiroz / Dos Santos, Flavia Barrento / Damasco, Paulo Vieira / da Cunha, Rivaldo Venancio / Azeredo, Elzinandes Leal de / de Oliveira-Pinto, Luzia Maria

Intervirology

2020 Volume 63, Issue 1-6, Page(s) 33–45

Abstract: Background: Arboviruses co-circulating within a population that are transmitted by the same vector have the potential to cause coinfections. Coinfections with dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV) have been occurring in ... ...

Abstract	Background: Arboviruses co-circulating within a population that are transmitted by the same vector have the potential to cause coinfections. Coinfections with dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV) have been occurring in Brazil, but it is not well-understood how human responses vary during mono- or coinfections and whether they play different roles in pathogenesis. Methods: We investigated the clinical, virological, and immunological status during patients' acute infections, focusing on the CCL/CXC chemokines, proinflammatory, as well as anti-inflammatory cytokines levels quantified by ELISAs. Viral load was determined by qRT-PCR in serum samples from 116 acute DENV, ZIKV, CHIKV, DENV/ZIKV, and CHIKV/ZIKV-infected adult patients from Brazil. Results: Most of the acute patients displayed fever, headache, prostration, and myalgia, regardless of the type of arbovirus infection. Zika viral load was higher in CHIKV/ZIKV coinfected patients compared with ZIKV or DENV/ZIKV infections. All infected individuals presented increased concentrations of C-X-C motif chemokine ligand 10/interferon protein-10 (CXCL10/IP-10), C-C motif chemokine ligand 2/monocyte chemoattractant protein-1 (CCL2/MCP-1), and tumor necrosis factor alpha (TNF-α) compared to healthy donors. Interestingly, the ZIKV group separated from CHIKV/ZIKV due to higher levels of interleukin-10 (IL-10) and lower levels of TNF-α. While DENV/ZIKV differentiated from CHIKV due to their higher levels of CCL2/MCP-1, in CHIKV- and CHIKV/ZIKV-infected patients, levels of CXC10/IP-10, CCL2/MCP-1, and migration inhibitory factor (MIF) were associated with CHIKV viral load. By contrast, in DENV/ZIKV- and CHIKV/ZIKV-infected patients, levels of CXCL10/IP-10, CCL2/MCP-1, and TNF-α showed a significant inverse correlation with ZIKV viral load. Conclusions: From all the circulating mediators measured, we detected differences of IL-10, TNF-α, and CCL2/MCP-1 between arbovirus groups. We hypothesize that CXC10/IP-10, CCL2/MCP-1, and MIF in the CHIKV-infected group could regulate the CHIKV viral load, while CXC10/IP-10, CCL2/MCP-1, and TNF-α in DENV/ZIKV, and CHIKV/ZIKV groups, could regulate ZIKV viral load.
MeSH term(s)	Adult ; Brazil ; Chemokines, CC/blood ; Chemokines, CXC/blood ; Chikungunya Fever/immunology ; Chikungunya Fever/virology ; Chikungunya virus/physiology ; Coinfection ; Cytokines/blood ; Dengue/immunology ; Dengue Virus/physiology ; Female ; Humans ; Male ; Middle Aged ; Viral Load ; Young Adult ; Zika Virus/physiology ; Zika Virus Infection/immunology ; Zika Virus Infection/virology
Chemical Substances	Chemokines, CC ; Chemokines, CXC ; Cytokines
Language	English
Publishing date	2020-09-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	184545-7
ISSN	1423-0100 ; 0300-5526
ISSN (online)	1423-0100
ISSN	0300-5526
DOI	10.1159/000510223
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Article ; Online: Differential Longevity of Memory CD4 and CD8 T Cells in a Cohort of the Mothers With a History of ZIKV Infection and Their Children.

Badolato-Corrêa, Jessica / Carvalho, Fabiana Rabe / Paiva, Iury Amancio / Familiar-Macedo, Débora / Dias, Helver Gonçalves / Pauvolid-Corrêa, Alex / Fernandes-Santos, Caroline / Lima, Monique da Rocha Queiroz / Gandini, Mariana / Silva, Andréa Alice / Baeta Cavalcanti, Silvia Maria / de Oliveira, Solange Artimos / de Oliveira Vianna, Renata Artimos / de Azeredo, Elzinandes Leal / Cardoso, Claudete Aparecida Araújo / Grifoni, Alba / Sette, Alessandro / Weiskopf, Daniela / de-Oliveira-Pinto, Luzia Maria

Frontiers in immunology

2021 Volume 12, Page(s) 610456

Abstract: Background: ...

Abstract	Background:
MeSH term(s)	Adult ; Antibodies, Neutralizing/immunology ; Antibodies, Viral/immunology ; CD4-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/metabolism ; Cross Reactions/immunology ; Cross-Sectional Studies ; Female ; Humans ; Immunity, Maternally-Acquired ; Immunologic Memory ; Immunophenotyping ; Neutralization Tests ; Pregnancy ; Pregnancy Complications, Infectious ; Young Adult ; Zika Virus/immunology ; Zika Virus Infection/blood ; Zika Virus Infection/epidemiology ; Zika Virus Infection/immunology
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral
Language	English
Publishing date	2021-02-12
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2021.610456
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Article ; Online: NS1 Antigenemia and Viraemia Load: Potential Markers of Progression to Dengue Fatal Outcome?

Nunes, Priscila Conrado Guerra / Nogueira, Rita Maria Ribeiro / Heringer, Manoela / Chouin-Carneiro, Thaís / Damasceno Dos Santos Rodrigues, Cintia / de Filippis, Ana Maria Bispo / Lima, Monique da Rocha Queiroz / Dos Santos, Flávia Barreto

Viruses

2018 Volume 10, Issue 6

Abstract: Dengue is a worldwide problem characterized by a multifactorial pathogenesis. Considering the viral components, it is known that high viremia or high levels of the secreted nonstructural protein 1 (NS1) may be associated with a more severe disease. We ... ...

Abstract	Dengue is a worldwide problem characterized by a multifactorial pathogenesis. Considering the viral components, it is known that high viremia or high levels of the secreted nonstructural protein 1 (NS1) may be associated with a more severe disease. We aimed to characterize the NS1 antigenemia and viremia in dengue fatal and non-fatal cases, as potential markers of progression to a fatal outcome. NS1 antigenemia and viremia were determined in Brazilian dengue fatal cases (
MeSH term(s)	Antigens, Viral/blood ; Biomarkers/blood ; Brazil ; Dengue/pathology ; Dengue/virology ; Dengue Virus/isolation & purification ; Humans ; Prognosis ; Survival Analysis ; Viral Load ; Viremia
Chemical Substances	Antigens, Viral ; Biomarkers
Language	English
Publishing date	2018-06-14
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v10060326
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Article ; Online: The inability of a dengue NS1 ELISA to detect Zika infections.

Lima, Monique da Rocha Queiroz / Chouin-Carneiro, Thais / Azeredo, Elzinandes / Barbosa, Luciana Santos / Souza, Thiara Manuele Alves / Silva, Jéssica Badolato Corrêa da / Nunes, Priscila Conrado Guerra / Dal Fabbro, Márcia / Facco, Izilyanne H Romanholi / Venâncio-da-Cunha, Rivaldo / Dos Santos, Flavia Barreto

Scientific reports

2019 Volume 9, Issue 1, Page(s) 18596

Abstract: The presence of dengue virus (DENV), Zika virus (ZIKV) and Chikungunya virus (CHIKV) in Brazil, may result in a difficult diagnosis due to the signs and symptoms shared by those. Moreover, as DENV and ZIKV belong to the same family, serological assays ... ...

Abstract	The presence of dengue virus (DENV), Zika virus (ZIKV) and Chikungunya virus (CHIKV) in Brazil, may result in a difficult diagnosis due to the signs and symptoms shared by those. Moreover, as DENV and ZIKV belong to the same family, serological assays may show a high rate of cross-reactivity. Here, we evaluated a Dengue NS1 capture assay for early and differential diagnosis of dengue during the Zika epidemic occurred in Brazil in 2016. Samples (n = 227) from 218 patients included sera, plasma and urine from previously confirmed acute cases of Zika, dengue and Zika/dengue co-infections. Nine of those patients presented two specimens. The Dengue NS1 test was very specific for dengue diagnosis (99.32%), even in the co-circulation with ZIKV, and exhibited a high accuracy in not detecting acute Zika infections (92.43%). Our findings showed that the dengue NS1 capture test analyzed here was not able to recognize the ZIKV NS1 and its potential for cross-reaction.
MeSH term(s)	Antibodies, Viral/immunology ; Brazil/epidemiology ; Cross Reactions ; Cross-Sectional Studies ; Dengue/diagnosis ; Dengue/immunology ; Dengue Virus ; Enzyme-Linked Immunosorbent Assay/methods ; Humans ; Reproducibility of Results ; Sensitivity and Specificity ; Serotyping ; Viral Nonstructural Proteins/analysis ; Viral Nonstructural Proteins/immunology ; Zika Virus ; Zika Virus Infection/diagnosis ; Zika Virus Infection/immunology
Chemical Substances	Antibodies, Viral ; NS1 protein, dengue-1 virus ; Viral Nonstructural Proteins
Language	English
Publishing date	2019-12-09
Publishing country	England
Document type	Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-019-55160-4
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Article ; Online: Comparison of three commercially available dengue NS1 antigen capture assays for acute diagnosis of dengue in Brazil.

Lima, Monique da Rocha Queiroz / Nogueira, Rita Maria Ribeiro / Schatzmayr, Hermann Gonçalves / dos Santos, Flavia Barreto

PLoS neglected tropical diseases

2010 Volume 4, Issue 7, Page(s) e738

Abstract: Background: Dengue is associated with explosive urban epidemics and has become a major public health problem in many tropical developing countries, including Brazil. The laboratory diagnosis of dengue can be carried out using several approaches, however ...

Abstract	Background: Dengue is associated with explosive urban epidemics and has become a major public health problem in many tropical developing countries, including Brazil. The laboratory diagnosis of dengue can be carried out using several approaches, however sensitive and specific assays useful to diagnose in the early stage of fever are desirable. The flavivirus non-structural protein NS1, a highly conserved and secreted glycoprotein, is a candidate protein for rapid diagnosis of dengue in endemic countries. Methodology/principal findings: We aimed to evaluate the potential use of 3 commercial kits in a panel of 450 serum samples for early diagnosis of dengue in Brazil. The PanBio Early ELISA (PanBio Diagnostics) showed a sensitivity of 72.3% (159/220) and a specificity of 100%, while the sensitivity of the Platelia NS1 assay (Biorad Laboratories) was 83.6% (184/220). However, the highest sensitivity (89.6%; 197/220) was obtained by using the NS1 Ag Strip (Biorad Laboratories). A lower sensitivity was observed in DENV-3 cases by all 3 kits. Serum positive by virus isolation were more often positive than cases positive by RT-PCR by all three assays and a higher detection rate was observed during the first four days after the onset of the symptoms. The presence or absence of IgM showed no influence in the confirmation by the pan-E Early ELISA (P = 0,6159). However, a higher confirmation by both Platelia NS1 (Biorad) and Dengue NS1 Ag Strip (Biorad) in the absence of IgM was statistically significant (P<0,0001 and P = 0,0008, respectively). Only the Platelia NS1 test showed a higher sensitivity in confirming primary infections than secondary ones. Conclusions/significance: The results indicate that commercial kits of dengue NS1 antigen are useful for the laboratory diagnosis of acute primary and secondary dengue. It can be used in combination with the MAC-ELISA for case detection and as screening test to complement viral isolation.
MeSH term(s)	Antibodies, Viral/blood ; Brazil ; Dengue/diagnosis ; Humans ; Immunoassay/methods ; Immunoglobulin M/blood ; RNA, Viral/blood ; Viral Nonstructural Proteins/blood ; Virology/methods
Chemical Substances	Antibodies, Viral ; Immunoglobulin M ; NS1 protein, Flavivirus ; RNA, Viral ; Viral Nonstructural Proteins
Language	English
Publishing date	2010-07-06
Publishing country	United States
Document type	Comparative Study ; Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2429704-5
ISSN	1935-2735 ; 1935-2727
ISSN (online)	1935-2735
ISSN	1935-2727
DOI	10.1371/journal.pntd.0000738
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Comparison of two generations of the Panbio dengue NS1 capture enzyme-linked immunosorbent assay.

Lima, Monique da Rocha Queiroz / Nogueira, Rita Maria Ribeiro / Bispo de Filippis, Ana Maria / dos Santos, Flavia Barreto

Clinical and vaccine immunology : CVI

2011 Volume 18, Issue 6, Page(s) 1031–1033

Abstract: We compared two generations of Panbio (Brisbane, Australia) commercial kits for NS1 antigen capture for early diagnosis of dengue: the first-generation pan-E Dengue Early ELISA and the second-generation Dengue Early ELISA. The test improvement resulted ... ...

Abstract	We compared two generations of Panbio (Brisbane, Australia) commercial kits for NS1 antigen capture for early diagnosis of dengue: the first-generation pan-E Dengue Early ELISA and the second-generation Dengue Early ELISA. The test improvement resulted in a highly sensitive and specific test suitable for use as a first-line test in the field.
MeSH term(s)	Australia ; Clinical Laboratory Techniques/methods ; Dengue/diagnosis ; Enzyme-Linked Immunosorbent Assay/methods ; Humans ; Sensitivity and Specificity ; Viral Nonstructural Proteins/blood
Chemical Substances	Viral Nonstructural Proteins
Language	English
Publishing date	2011-04-27
Publishing country	United States
Document type	Comparative Study ; Evaluation Study ; Journal Article
ZDB-ID	2221082-9
ISSN	1556-679X ; 1556-6811
ISSN (online)	1556-679X
ISSN	1556-6811
DOI	10.1128/CVI.00024-11
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