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  1. AU="Limin Huang"
  2. AU="Torabfam Milad"
  3. AU=Hosny Somaya
  4. AU="Mercieca, Janelle"
  5. AU="Walker, Geoffrey Scott"
  6. AU="Márquez-Sánchez, M T"
  7. AU="Remy, Séverine"
  8. AU="van der Stoel, Miesje M"
  9. AU="Hifumi, Toru"
  10. AU="Jie Chen"
  11. AU="Ballarin, Barbara"
  12. AU="Thiel, Nadine"
  13. AU="Gerti Pishtari"
  14. AU="Alexander Hammers, ProfPhD"
  15. AU="Dessimoz, Christophe"
  16. AU="Planchuelo-Gómez, Álvaro"
  17. AU="McKay, Lindsay G A"
  18. AU="Laude, Marie-Charlotte"
  19. AU="Hale, Lillian V A"
  20. AU="Hassan Hartman"
  21. AU="Song, Mengwei"
  22. AU="Yimin Geng"
  23. AU="Wang, Chang-Sheng"
  24. AU="Doyle, Andrew D"
  25. AU="Redpath, Sophie H A"
  26. AU="Hopman, Maria Te"
  27. AU="Pomputius, William"
  28. AU="Agrawal, Sonali"
  29. AU="Martinez, Luis R"
  30. AU="Passoni, Lorena"
  31. AU="Slimani, Wafa"
  32. AU="Jin, J"
  33. AU="Xia, Hongmin"
  34. AU="Akdemir, İrem"
  35. AU=Ciccone Giovannino

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  1. Artikel: Curcumin interrupts leptin-regulated microRNA-122 in hepatic stellate cells in vitro and in vivo.

    Limin, Huang / Feifan, Xu

    Pakistan journal of pharmaceutical sciences

    2023  Band 36, Heft 2, Seite(n) 417–423

    Abstract: The purpose of this study was to evaluate the effect of curcumin, an active polyphenol, on the leptin induced lowering of miR-122 in Hepatic stellate cells (HSCs) in vivo and an animal model. Gene expression was evaluated by transfection assay, real-time ...

    Abstract The purpose of this study was to evaluate the effect of curcumin, an active polyphenol, on the leptin induced lowering of miR-122 in Hepatic stellate cells (HSCs) in vivo and an animal model. Gene expression was evaluated by transfection assay, real-time PCR, or Western blot analysis. The liver fibrosis model of leptin deficient mouse was used for in vivo experiment. As a result, curcumin showed inhibitory effect on leptin induced lowering of the miR-122 in HSCs. Curcumin suppressed leptin induced sonic hedgehog (Shh) expression and blocked leptin induced Shh signaling pathway, which was essential for curcumin inhibition of the negative role of leptin in miR-122 expression in HSCs. The influence of curcumin on the negative effect of leptin on miR-122 level was followed by the attenuation of liver fibrosis caused by leptin in leptin-deficient mouse model. In conclusion, curcumin could reduce the decrease of miR-122 level in HSCs induced by leptin and inhibit liver fibrosis induced by leptin. These data may have potential implications to treat with liver fibrosis by elevating the expression of leptin in humans especially obese patients.
    Mesh-Begriff(e) Mice ; Humans ; Animals ; Hepatic Stellate Cells ; Curcumin/adverse effects ; Leptin/metabolism ; Hedgehog Proteins/metabolism ; Hedgehog Proteins/pharmacology ; Disease Models, Animal ; Liver Cirrhosis/chemically induced ; Liver Cirrhosis/drug therapy ; MicroRNAs/metabolism
    Chemische Substanzen Curcumin (IT942ZTH98) ; Leptin ; Hedgehog Proteins ; MicroRNAs ; MIRN122 microRNA, human ; Mirn122 microRNA, mouse
    Sprache Englisch
    Erscheinungsdatum 2023-06-30
    Erscheinungsland Pakistan
    Dokumenttyp Journal Article
    ZDB-ID 885131-1
    ISSN 1011-601X
    ISSN 1011-601X
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: A missense mutant of ocrl1 promotes apoptosis of tubular epithelial cells and disrupts endocytosis and the cell cycle of podocytes in Dent-2 Disease

    Limin Huang / Yingying Zhang / Haidong Fu / Weizhong Gu / Jianhua Mao

    Cell Communication and Signaling, Vol 21, Iss 1, Pp 1-

    2023  Band 15

    Abstract: Abstract Background This study aimed to identify an orcl1 mutation in a patient with Dent-2 Disease and investigate the underlying mechanisms. Methods The ocrl1 mutation was identified through exome sequencing. Knockdown of orcl1 and overexpression of ... ...

    Abstract Abstract Background This study aimed to identify an orcl1 mutation in a patient with Dent-2 Disease and investigate the underlying mechanisms. Methods The ocrl1 mutation was identified through exome sequencing. Knockdown of orcl1 and overexpression of the orcl1 mutant were performed in HK-2 and MPC5 cells to study its function, while flow cytometry measured reactive oxygen species (ROS), phosphatidylserine levels, and cell apoptosis. Scanning electron microscopy observed crystal adhesion, while transmission electron microscopy examined kidney tissue pathology. Laser scanning confocal microscopy was used to examine endocytosis, and immunohistochemical and immunofluorescence assays detected protein expression. Additionally, podocyte-specific orcl1 knockout mice were generated to investigate the role of orcl1 in vivo. Results We identified a mutation resulting in the replacement of Histidine with Arginine at position 318 (R318H) in ocrl1 in the proband. orcl1 was widely expressed in the kidney. In vitro experiments showed that knockdown of orcl1 and overexpression of ocrl1 mutant increased ROS, phosphatidylserine exocytosis, crystal adhesion, and cell apoptosis in HK-2 cells. Knockdown of orcl1 in podocytes reduced endocytosis and disrupted the cell cycle while increasing cell migration. In vivo studies in mice showed that conditional deletion of orcl1 in podocytes caused glomerular dysfunction, including proteinuria and fibrosis. Conclusion This study identified an R318H mutation in orcl1 in a patient with Dent-2 Disease. This mutation may contribute to renal injury by promoting ROS production and inducing cell apoptosis in tubular cells, while disrupting endocytosis and the cell cycle, and promoting cell migration of podocytes. Video Abstract
    Schlagwörter Lowe syndrome ; Dent disease ; Whole exome sequencing ; Podocytes ; Cell cycle ; Renal tubular ; Medicine ; R ; Cytology ; QH573-671
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2023-12-01T00:00:00Z
    Verlag BMC
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Screening of disease-related biomarkers related to neuropathic pain (NP) after spinal cord injury (SCI)

    Jia Zhao / Li Yang / Limin Huang / Zinan Li

    Human Genomics, Vol 15, Iss 1, Pp 1-

    2021  Band 12

    Abstract: Abstract Background Based on the molecular expression level, this paper compares lncRNA and mRNA expressions respectively in peripheral blood samples of the patients after SCI with NP and without NP, and screens disease-related biomarkers related to NP ... ...

    Abstract Abstract Background Based on the molecular expression level, this paper compares lncRNA and mRNA expressions respectively in peripheral blood samples of the patients after SCI with NP and without NP, and screens disease-related biomarkers related to NP after SCI in peripheral blood samples of patients. Method The expression spectrum of 25 human peripheral blood samples (12 samples of refractory NP patients after SCI) was downloaded and data were normalized. Screening of GO annotations significantly associated with significant differentially expressed mRNAs and significant involvement of the KEGG pathway. The WGCNA algorithm was used to screen for modules and RNAs that were significantly associated with disease characterization. A co-expression network was constructed to extract the genes involved in the disease pathway from the co-expression network, construct a network of SCI pain-related pathways, and screen important disease-related biomarkers. Quantitative real-time PCR was used to detect the mRNA expression of hub genes. Results Data were normalized and re-annotated by detection of platform information, resulting in a total of 289 lncRNA and 18197 mRNAs. Screening resulted in 338 significant differentially expressed RNAs that met the threshold requirements. Differentially expressed RNAs were significantly enriched with the brown and magenta modules. Six KEGG signaling pathways were screened in the co-expression network, and three KEGG pathways with direct neuropathic pain were identified. The expression levels of E2F1, MAX, MITF, CTNNA1, and ADORA2B in the disease group were all significantly upregulated (p < 0.01). Compared with the normal group, the expression of OXTR was upregulated. Conclusion We speculate that there are 7 genes and 2 lncRNAs directly involved in the pain pathway: E2F1, MAX, MITF, CTNNA1, ADORA2B, GRIK3, OXTR, LINC01119, and LINC02447. These molecules may be important for NP after SCI.
    Schlagwörter Spinal cord injury (SCI) ; Neuropathic pain (NP) ; Disease-related biomarkers ; Medicine ; R ; Genetics ; QH426-470
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2021-01-01T00:00:00Z
    Verlag BMC
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: Numerical Investigations on Wave Remote Sensing from Synthetic X-Band Radar Sea Clutter Images by Using Deep Convolutional Neural Networks

    Wenyang Duan / Ke Yang / Limin Huang / Xuewen Ma

    Remote Sensing, Vol 12, Iss 1117, p

    2020  Band 1117

    Abstract: X-band marine radar is an effective tool for sea wave remote sensing. Conventional physical-based methods for acquiring wave parameters from radar sea clutter images use three-dimensional Fourier transform and spectral analysis. They are limited by some ... ...

    Abstract X-band marine radar is an effective tool for sea wave remote sensing. Conventional physical-based methods for acquiring wave parameters from radar sea clutter images use three-dimensional Fourier transform and spectral analysis. They are limited by some assumptions, empirical formulas and the calibration process while obtaining the modulation transfer function (MTF) and signal-to-noise ratio (SNR). Therefore, further improvement of wave inversion accuracy by using the physical-based method presents a challenge. Inspired by the capability of convolutional neural networks (CNN) in image characteristic processing, a deep-learning inversion method based on deep CNN is proposed. No intermediate step or parameter is needed in the CNN-based method, therefore fewer errors are introduced. Wave parameter inversion models were constructed based on CNN to inverse the wave’s spectral peak period and significant wave height. In the present paper, the numerically simulated X-band radar image data were used for a numerical investigation of wave parameters. Results of the conventional spectral analysis and CNN-based methods were compared and the CNN-based method had a higher accuracy on the same data set. The influence of training strategy on CNN-based inversion models was studied to analyze the dependence of a deep-learning inversion model on training data. Additionally, the effects of target parameters on the inversion accuracy of CNN-based models was also studied.
    Schlagwörter wave parameter inversion ; X-band radar sea clutter ; deep-learning method ; CNN model ; training data dependence ; Science ; Q
    Thema/Rubrik (Code) 518
    Sprache Englisch
    Erscheinungsdatum 2020-04-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
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  5. Artikel ; Online: The analysis of fertility quality of life and the influencing factors of patients with repeated implantation failure

    Ying Ni / Chenye Tong / Limin Huang / Wenjie Zhou / Aijun Zhang

    Health and Quality of Life Outcomes, Vol 19, Iss 1, Pp 1-

    2021  Band 10

    Abstract: Abstract Background The objective of this study was to investigate the current status of fertility quality of life (QoL) and explore the influencing factors for infertile women with repeated implantation failure (RIF). Methods The sample consisted of 137 ...

    Abstract Abstract Background The objective of this study was to investigate the current status of fertility quality of life (QoL) and explore the influencing factors for infertile women with repeated implantation failure (RIF). Methods The sample consisted of 137 infertile women with RIF who were under treatment from January 2019 to December 2019 in the Reproductive Medical Center of Ruijin Hospital, affiliated with Shanghai Jiaotong University School of Medicine in China. A general information questionnaire, FertiQoL scale, perceived social support scale (PSSS), self-rating anxiety scale (SAS), and self-rating depression scale (SDS) were used to analyse the fertility QoL and related factors of RIF patients. Results The total fertility QoL score of RIF patients was 60.44 ± 11.60. The results of multivariate regression analysis showed that residence, financial difficulties, male infertility, BMI index, depression, and family social support were the main factors that influenced the fertility QoL of RIF patients (adjusted R2 = 0.762). Conclusion Based on the results of this study, RIF patients’ psychological status must be addressed. Corresponding interventions such as building a sound family and social support system, creating a good medical environment and offering diverse health education should be provided to improve the fertility QoL of RIF patients.
    Schlagwörter Repeated implantation failure ; Fertility quality of life ; Social support ; Anxiety ; Depression ; Influencing factors ; Computer applications to medicine. Medical informatics ; R858-859.7
    Thema/Rubrik (Code) 360
    Sprache Englisch
    Erscheinungsdatum 2021-01-01T00:00:00Z
    Verlag BMC
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: Molecular epidemiology and clinical characteristics of respiratory syncytial virus in hospitalized children during winter 2021–2022 in Bengbu, China

    Limin Huang / Yuanyou Xu / Yanqing Yang / Hongming Dong / Qin Luo / Zhen Chen / Haijun Du / Guoyong Mei / Xinyue Wang / Yake Guan / Chihong Zhao / Jun Han / Guoyu Lu

    Frontiers in Public Health, Vol

    2024  Band 11

    Abstract: ObjectiveThis study aimed to study the molecular epidemiology and clinical characteristics of respiratory syncytial virus (RSV) infection from hospitalized children with ARTI in Bengbu.MethodsOne hundred twenty-four nasopharyngeal swab specimens and ... ...

    Abstract ObjectiveThis study aimed to study the molecular epidemiology and clinical characteristics of respiratory syncytial virus (RSV) infection from hospitalized children with ARTI in Bengbu.MethodsOne hundred twenty-four nasopharyngeal swab specimens and clinical data from children with ARTI cases were collected in Bengbu, China, during winter 2021–2022. The samples were detected by qPCR of 13 respiratory viruses. Phylogenetic analysis was constructed using MEGA 7.0. All analyses were performed using SAS software, version 9.4.ResultsIn winter 2021–2022, URTI, NSCAP, SCAP, and bronchiolitis accounted for 41.03%, 27.35%, 17.09%, and 14.53% of hospitalized children in Bengbu, China. The detection rates of the top three were RSV (41.94%), ADV (5.65%), and FluB (5.65%) in hospitalized children through 13 virus detection. RSV is the main pathogen of hospitalized children under 2 years old. Forty-eight sequences of G protein of RSV were obtained through PCR amplification, including RSV-A 37 strains and RSV-B 11 strains. Phylogenetic analysis showed that all RSV-A and RSV-B were ON1 and BA9 genotypes, respectively. ON1 genotypes were further divided into two clades. The majority of ON1 strains formed a unique genetic clade with T113I, V131D, N178 G, and H258Q mutations. Furthermore, RSV infection was an independent risk factor for ventilator use (OR = 9.55, 95% CI 1.87–48.64).ConclusionThere was a high incidence of RSV among hospitalized children during winter 2021–2022 in Bengbu with ON1 and BA9 being the dominant strains. This study demonstrated the molecular epidemiological characteristics of RSV in children with respiratory infections in Bengbu, China.
    Schlagwörter respiratory syncytial virus ; molecular epidemiology ; clinical characteristics ; G gene ; amino acid mutation ; Public aspects of medicine ; RA1-1270
    Sprache Englisch
    Erscheinungsdatum 2024-01-01T00:00:00Z
    Verlag Frontiers Media S.A.
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  7. Artikel ; Online: Clinicopathological Significance of Neuropilin 1 Expression in Gastric Cancer

    Hui Cao / Yan Li / Limin Huang / Banjun Bai / Zhong Xu

    Disease Markers, Vol

    A Meta-Analysis

    2020  Band 2020

    Abstract: Background. Neuropilin 1 (NRP1) is involved in tumorigenesis, development, invasion, and metastasis by promoting angiogenesis of tumors. The study is aimed at evaluating the correlation between the expression of NRP1 protein and clinicopathological ... ...

    Abstract Background. Neuropilin 1 (NRP1) is involved in tumorigenesis, development, invasion, and metastasis by promoting angiogenesis of tumors. The study is aimed at evaluating the correlation between the expression of NRP1 protein and clinicopathological features of gastric cancer by meta-analysis. Methods. The published studies were searched in databases including CNKI, Wanfang, Chongqing VIP, Web of Science, and PubMed online. Clinical case studies were included to compare the correlation between NRP1 protein expression and clinicopathological characteristics of gastric cancer. The quality of the included literatures was evaluated by NOS scale. Meta-analysis was performed by Stata software to calculate the odds ratio (OR) and 95% confidence interval (CI). Results. A total of 12 studies were included in this analysis, involving 1,225 patients with gastric cancer. The analysis indicated that the expression of NRP1 protein in gastric cancer tissues was lower in the group of early stage versus advanced stage (OR=0.128, 95%CI=0.059−0.277, P≤0.001), tumor size less than 5 cm versus more than 5 cm (OR=0.443, 95%CI=0.310−0.632, P≤0.001), TNM stage I-II group versus stage III-IV patients (OR=0.736, 95%CI=0.589−0.919, P=0.007), well to medium differentiation group versus poor differentiation group (OR=0.735, 95%CI=0.632−0.854, P≤0.001), and nonlymph node metastasis group versus lymph node metastasis group (OR=0.667, 95%CI=0.522−0.854, P≤0.001). The expression of NRP1 protein in gastric cancer was not related to gender, age, and Laurèn’s classification. Conclusion. The expression of NRP1 protein in gastric cancer is closely correlated to clinical stage, tumor size, TNM stage, differentiation, and lymph node metastasis.
    Schlagwörter Medicine (General) ; R5-920
    Thema/Rubrik (Code) 616
    Sprache Englisch
    Erscheinungsdatum 2020-01-01T00:00:00Z
    Verlag Hindawi Limited
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel ; Online: Maternal experience of intermittent kangaroo mother care for late preterm infants

    Jun Liu / Yingxi Zhao / Sarah Williams / Lin Zhang / Wenli Wu / Jieya Yue / Zhiying Duan / Limin Huang / Xiaoqin Zhang / Gengli Zhao

    BMJ Open, Vol 11, Iss

    a mixed-methods study in four postnatal wards in China

    2021  Band 9

    Schlagwörter Medicine ; R
    Sprache Englisch
    Erscheinungsdatum 2021-09-01T00:00:00Z
    Verlag BMJ Publishing Group
    Dokumenttyp Artikel ; Online
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  9. Artikel ; Online: A Touch-Communication Framework for Drug Delivery Based on a Transient Microbot System.

    Yifan Chen / Kosmas, Panagiotis / Anwar, Putri Santi / Limin Huang

    IEEE transactions on nanobioscience

    2015  Band 14, Heft 4, Seite(n) 397–408

    Abstract: Recent progress in bioresorbable radio frequency electronics and engineered bacteria has promised the prospect of realizing a transient microbot (TM) system for therapeutic applications. The inorganic or organic miniature robots will dissolve into the ... ...

    Abstract Recent progress in bioresorbable radio frequency electronics and engineered bacteria has promised the prospect of realizing a transient microbot (TM) system for therapeutic applications. The inorganic or organic miniature robots will dissolve into the human body after completing the required tasks and cause no side-effect. In this paper, we propose a potential architecture of a TM system for transporting pharmaceutical compounds inside the body, and analyze the system using a micro-to-macro cross-scale communication model. The remote controllability and tangibility of a TM essentially lead to a touch-communication (TouchCom) paradigm. Externally maneuverable and trackable TMs are responsible for the delivery of drug particles (information molecules in the TouchCom context). The loading/injection and unloading of the drug correspond to the transmitting and receiving processes in the TouchCom framework. Subsequently, we investigate simulation tools for the propagation and transient characteristics of TMs in the blood vessels. We also define the propagation delay, path loss, as well as angular and delay spectra of targeting intensity, which are parallel to their counterpart concepts in the conventional wireless channel. Finally, our approach is illustrated with comprehensive simulation studies of targeted drug delivery by using the proposed analytical framework integrating robotics and communications at crossover length scales. The proposed methodology may find important applications in the design and analysis of TM-assisted administration of pharmaceutical compounds.
    Sprache Englisch
    Erscheinungsdatum 2015-06
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 1558-2639
    ISSN (online) 1558-2639
    DOI 10.1109/TNB.2015.2395539
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel: Quantification of the limitation of Langmuir model used in adsorption research on sediments via site energy heterogeneity

    Jin, Qiang / Limin Huang / Aimin Li / Aidang Shan

    Chemosphere. 2017 Oct., v. 185

    2017  

    Abstract: The Langmuir model has been extensively introduced into the field of environmental adsorption, while some studies showed that it was difficult for the model to describe the adsorption of sediments. The purpose of this paper is to recognize the ... ...

    Abstract The Langmuir model has been extensively introduced into the field of environmental adsorption, while some studies showed that it was difficult for the model to describe the adsorption of sediments. The purpose of this paper is to recognize the applicability of the Langmuir model used in the adsorption of contaminants onto sediments quantitatively through the relationship between the error of Langmuir (δ) and site energy heterogeneity (σ). The formula for calculating δ in sediments was developed based on the heterogeneity parameters (m, n). The data was extracted from papers discussing about the adsorption of pollutants on natural sediments. It was further used to investigate the error of Langmuir and the effect on the error from the site energy heterogeneity. The results indicate that the Langmuir model can be applied in sediments when each one of the conditions below is satisfied, (1) m and n lie in the area which signifies that the relative error is less than 10%, (2) the site energy heterogeneity of sediment is under 5.668. These findings are vital for the proper choice of models fitting the adsorption process of sediments.
    Schlagwörter adsorption ; energy ; models ; pollutants ; sediments ; sorption isotherms
    Sprache Englisch
    Erscheinungsverlauf 2017-10
    Umfang p. 518-528.
    Erscheinungsort Elsevier Ltd
    Dokumenttyp Artikel
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2017.07.051
    Datenquelle NAL Katalog (AGRICOLA)

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