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  1. Article ; Online: Potential mechanism of transient receptor potential cation channel subfamily V member 1 combined with an ATP‑sensitive potassium channel in severe preeclampsia.

    Zhou, Xianyi / Lin, Hairui / Wei, Li / Tan, Yingyun / Fu, Xiaodong

    Experimental and therapeutic medicine

    2023  Volume 26, Issue 1, Page(s) 318

    Abstract: Severe preeclampsia is one of the most serious obstetric diseases. However, the pathogenesis of the disease is not fully understood. In the present study, placental artery and blood serum was collected from patients with severe preeclampsia, as well as ... ...

    Abstract Severe preeclampsia is one of the most serious obstetric diseases. However, the pathogenesis of the disease is not fully understood. In the present study, placental artery and blood serum was collected from patients with severe preeclampsia, as well as from normal pregnant women. The results of reverse transcription-quantitative (q)PCR, western blotting, and immunohistochemical staining revealed markedly decreased transient receptor potential cation channel subfamily V member 1 (TRPV1), ATP-sensitive potassium channel (KATP) subtype Kir6.1/SUR2B and endothelial nitric oxide synthase (eNOS) expression in severe preeclampsia tissue specimens compared with those in samples from normal pregnant women. The nitrate reduction method indicated lower NO levels in the tissue specimens and serum of patients with severe preeclampsia. Moreover, hematoxylin-eosin staining showed that the endothelial cell layer in the placental artery of patients with severe preeclampsia was notably damaged. To investigate the potential role of TRPV1-KATP channels in severe preeclampsia, HUVECs were used for
    Language English
    Publishing date 2023-05-15
    Publishing country Greece
    Document type Journal Article
    ZDB-ID 2683844-8
    ISSN 1792-1015 ; 1792-0981
    ISSN (online) 1792-1015
    ISSN 1792-0981
    DOI 10.3892/etm.2023.12017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Multimodal Brain Image Fusion Based on Improved Rolling Guidance Filter and Wiener Filter.

    Lin, Hairui / Song, Yuhang / Wang, Hua / Xie, Luoxin / Li, Dongfen / Yang, Guocheng

    Computational and mathematical methods in medicine

    2022  Volume 2022, Page(s) 5691099

    Abstract: Medical image fusion technology can integrate complementary information from different modality medical images to provide a more complete and accurate description of the specific diagnosed object, which is very helpful for image-guided clinical diagnosis ...

    Abstract Medical image fusion technology can integrate complementary information from different modality medical images to provide a more complete and accurate description of the specific diagnosed object, which is very helpful for image-guided clinical diagnosis and treatment. This paper proposes an effective brain image fusion framework based on improved rolling guidance filter (IRGF). Firstly, input images are decomposed into base layers and detail layers using the IRGF and Wiener filter. Secondly, the visual saliency maps of the input image are computed by pixel-level saliency value, and the weight maps of detail layers are constructed by max-absolute strategy and are further smoothed with Gaussian filter, the purpose of which is to make the fused image appear more naturally and more suitable for human visual perception. Lastly, base layers are fused by visual saliency map based fusion rule and the corresponding weight maps from detail layers are fused by the weighted least squares optimization scheme. Experimental results testify that our method is superior to some state-of-the-art methods in both subjective and objective assessments.
    MeSH term(s) Humans ; Image Processing, Computer-Assisted/methods ; Magnetic Resonance Imaging/methods ; Algorithms ; Brain/diagnostic imaging ; Normal Distribution
    Language English
    Publishing date 2022-10-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2252430-7
    ISSN 1748-6718 ; 1748-670X ; 1027-3662
    ISSN (online) 1748-6718
    ISSN 1748-670X ; 1027-3662
    DOI 10.1155/2022/5691099
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6373: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Intermediate-Conductance-Ca2-Activated K Channel IKCa1 Is Upregulated and Promotes Cell Proliferation in Cervical Cancer

    Liu, Ling / Zhan, Ping / Nie, Dan / Fan, Lingye / Lin, Hairui / Gao, Lanyang / Mao, Xiguang

    Medical science monitor basic research

    2017  Volume 23, Page(s) 45–57

    Abstract: BACKGROUND Accumulating data point to intermediate-conductance calcium-activated potassium channel (IKCa1) as a key player in controlling cell cycle progression and proliferation of human cancer cells. However, the role that IKCa1 plays in the growth of ... ...

    Abstract BACKGROUND Accumulating data point to intermediate-conductance calcium-activated potassium channel (IKCa1) as a key player in controlling cell cycle progression and proliferation of human cancer cells. However, the role that IKCa1 plays in the growth of human cervical cancer cells is largely unexplored. MATERIAL AND METHODS In this study, Western blot analysis, immunohistochemical staining, and RT-PCR were first used for IKCa1protein and gene expression assays in cervical cancer tissues and HeLa cells. Then, IKCa1 channel blocker and siRNA were employed to inhibit the functionality of IKCa1 and downregulate gene expression in HeLa cells, respectively. After these treatments, we examined the level of cell proliferation by MTT method and measured IKCa1 currents by conventional whole-cell patch clamp technique. Cell apoptosis was assessed using the Annexin V-FITC/Propidium Iodide (PI) double-staining apoptosis detection kit. RESULTS We demonstrated that IKCa1 mRNA and protein are preferentially expressed in cervical cancer tissues and HeLa cells. We also showed that the IKCa1 channel blocker, clotrimazole, and IKCa1 channel siRNA can be used to suppress cervical cancer cell proliferation and decrease IKCa1 channel current. IKCa1 downregulation by specific siRNAs induced a significant increase in the proportion of apoptotic cells in HeLa cells. CONCLUSIONS IKCa1 is overexpressed in cervical cancer tissues, and IKCa1 upregulation in cervical cancer cell linea enhances cell proliferation, partly by reducing the proportion of apoptotic cells.
    MeSH term(s) Adult ; Calcium Channel Blockers/pharmacology ; Cell Proliferation/drug effects ; Cell Proliferation/physiology ; Clotrimazole/pharmacology ; Female ; Gene Expression Regulation, Neoplastic ; HeLa Cells ; Humans ; Intermediate-Conductance Calcium-Activated Potassium Channels/biosynthesis ; Intermediate-Conductance Calcium-Activated Potassium Channels/genetics ; Intermediate-Conductance Calcium-Activated Potassium Channels/metabolism ; Middle Aged ; Patch-Clamp Techniques ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Uterine Cervical Neoplasms/genetics ; Uterine Cervical Neoplasms/metabolism ; Uterine Cervical Neoplasms/pathology
    Chemical Substances Calcium Channel Blockers ; Intermediate-Conductance Calcium-Activated Potassium Channels ; KCNN4 protein, human ; RNA, Messenger ; Clotrimazole (G07GZ97H65)
    Language English
    Publishing date 2017-03-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2711344-9
    ISSN 2325-4416 ; 2325-4416
    ISSN (online) 2325-4416
    ISSN 2325-4416
    DOI 10.12659/msmbr.901462
    Database MEDical Literature Analysis and Retrieval System OnLINE

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