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  1. Article ; Online: RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis.

    Liu, Chao / Wang, Liying / Li, Yanan / Guo, Mengmeng / Hu, Jun / Wang, Teng / Li, Mengjing / Yang, Zhuo / Lin, Ruoyao / Xu, Wei / Chen, Yinghong / Luo, Mengcheng / Gao, Fei / Chen, Jia-Yu / Sun, Qianwen / Liu, Hongbin / Sun, Bo / Li, Wei

    Nucleic acids research

    2023  Volume 51, Issue 14, Page(s) 7357–7375

    Abstract: DNA-RNA hybrids play various roles in many physiological progresses, but how this chromatin structure is dynamically regulated during spermatogenesis remains largely unknown. Here, we show that germ cell-specific knockout of Rnaseh1, a specialized enzyme ...

    Abstract DNA-RNA hybrids play various roles in many physiological progresses, but how this chromatin structure is dynamically regulated during spermatogenesis remains largely unknown. Here, we show that germ cell-specific knockout of Rnaseh1, a specialized enzyme that degrades the RNA within DNA-RNA hybrids, impairs spermatogenesis and causes male infertility. Notably, Rnaseh1 knockout results in incomplete DNA repair and meiotic prophase I arrest. These defects arise from the altered RAD51 and DMC1 recruitment in zygotene spermatocytes. Furthermore, single-molecule experiments show that RNase H1 promotes recombinase recruitment to DNA by degrading RNA within DNA-RNA hybrids and allows nucleoprotein filaments formation. Overall, we uncover a function of RNase H1 in meiotic recombination, during which it processes DNA-RNA hybrids and facilitates recombinase recruitment.
    MeSH term(s) Humans ; Male ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; DNA/genetics ; DNA/metabolism ; Meiosis ; Rad51 Recombinase/genetics ; Rad51 Recombinase/metabolism ; Recombinases/genetics ; Spermatocytes/metabolism ; Ribonuclease H/metabolism
    Chemical Substances Cell Cycle Proteins ; DNA (9007-49-2) ; Rad51 Recombinase (EC 2.7.7.-) ; Recombinases ; ribonuclease HI (EC 3.1.26.4) ; Ribonuclease H (EC 3.1.26.4)
    Language English
    Publishing date 2023-08-14
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkad524
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1067: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: R-loopBase: a knowledgebase for genome-wide R-loop formation and regulation.

    Lin, Ruoyao / Zhong, Xiaoming / Zhou, Yongli / Geng, Huichao / Hu, Qingxi / Huang, Zhihao / Hu, Jun / Fu, Xiang-Dong / Chen, Liang / Chen, Jia-Yu

    Nucleic acids research

    2021  Volume 50, Issue D1, Page(s) D303–D315

    Abstract: R-loops play versatile roles in many physiological and pathological processes, and are of great interest to scientists in multiple fields. However, controversy about their genomic localization and incomplete understanding of their regulatory network ... ...

    Abstract R-loops play versatile roles in many physiological and pathological processes, and are of great interest to scientists in multiple fields. However, controversy about their genomic localization and incomplete understanding of their regulatory network raise great challenges for R-loop research. Here, we present R-loopBase (https://rloopbase.nju.edu.cn) to tackle these pressing issues by systematic integration of genomics and literature data. First, based on 107 high-quality genome-wide R-loop mapping datasets generated by 11 different technologies, we present a reference set of human R-loop zones for high-confidence R-loop localization, and spot conservative genomic features associated with R-loop formation. Second, through literature mining and multi-omics analyses, we curate the most comprehensive list of R-loop regulatory proteins and their targeted R-loops in multiple species to date. These efforts help reveal a global regulatory network of R-loop dynamics and its potential links to the development of cancers and neurological diseases. Finally, we integrate billions of functional genomic annotations, and develop interactive interfaces to search, visualize, download and analyze R-loops and R-loop regulators in a well-annotated genomic context. R-loopBase allows all users, including those with little bioinformatics background to utilize these data for their own research. We anticipate R-loopBase will become a one-stop resource for the R-loop community.
    MeSH term(s) Cell Line, Tumor ; Chromosome Mapping ; Computational Biology/methods ; DNA/chemistry ; DNA/genetics ; DNA/metabolism ; Databases, Nucleic Acid ; Datasets as Topic ; Gene Regulatory Networks ; Genome ; Genomic Instability ; HEK293 Cells ; Humans ; Internet ; Molecular Sequence Annotation ; Neoplasms/genetics ; Neoplasms/metabolism ; Neoplasms/pathology ; Nervous System Diseases/genetics ; Nervous System Diseases/metabolism ; Nervous System Diseases/pathology ; Protein Interaction Mapping/methods ; R-Loop Structures ; RNA/chemistry ; RNA/genetics ; RNA/metabolism ; Software ; Transcription, Genetic
    Chemical Substances RNA (63231-63-0) ; DNA (9007-49-2)
    Language English
    Publishing date 2021-12-16
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkab1103
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1067: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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