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  1. Article: Transcriptome Analysis Reveals Novel Insights into the Hyperaccumulator

    Xie, Qin / Deng, Wentao / Su, Yi / Ma, Liying / Yang, Haijun / Yao, Feihong / Lin, Wanhuang

    Plants (Basel, Switzerland)

    2024  Volume 13, Issue 2

    Abstract: Cadmium (Cd) is a highly toxic heavy metal that causes serious damage to plant and human health. ...

    Abstract Cadmium (Cd) is a highly toxic heavy metal that causes serious damage to plant and human health.
    Language English
    Publishing date 2024-01-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants13020297
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Multiple High-Affinity K+ Transporters and ABC Transporters Involved in K+ Uptake/Transport in the Potassium-Hyperaccumulator Plant Phytolacca acinosa Roxb

    Xie, Qin / Ma, Liying / Tan, Peng / Deng, Wentao / Huang, Chao / Liu, Deming / Lin, Wanhuang / Su, Yi

    Plants. 2020 Apr. 08, v. 9, no. 4

    2020  

    Abstract: Potassium is an important essential element for plant growth and development. Long-term potassium deprivation can lead to a severe deficiency phenotype in plants. Interestingly, Phytolacca acinosa is a plant with an unusually high potassium content and ... ...

    Abstract Potassium is an important essential element for plant growth and development. Long-term potassium deprivation can lead to a severe deficiency phenotype in plants. Interestingly, Phytolacca acinosa is a plant with an unusually high potassium content and can grow well and complete its lifecycle even in severely potassium deficient soil. In this study, we found that its stems and leaves were the main tissues for high potassium accumulation, and P. acinosa showed a strong ability of K⁺ absorption in roots and a large capability of potassium accumulation in shoots. Analysis of plant growth and physiological characteristics indicated that P. acinosa had an adaptability in a wide range of external potassium levels. To reveal the mechanism of K⁺ uptake and transport in the potassium-hyperaccumulator plant P. acinosa, K⁺ uptake-/transport-related genes were screened by transcriptome sequencing, and their expression profiles were compared between K⁺ starved plants and normal cultured plants. Eighteen members of HAK/KT/KUPs, ten members of AKTs, and one member of HKT were identified in P. acinosa. Among them, six HAKs, and two AKTs and PaHKT1 showed significantly different expression. These transporters might be coordinatively involved in K⁺ uptake/transport in P. acinosa and lead to high potassium accumulation in plant tissues. In addition, significantly changed expression of some ABC transporters indicated that ABC transporters might be important for K⁺ uptake and transport in P. acinosa under low K⁺ concentrations.
    Keywords ABC transporters ; Phytolacca acinosa ; absorption ; genes ; growth and development ; leaves ; phenotype ; plant development ; plant growth ; plant tissues ; potassium ; roots ; shoots ; soil ; stems ; transcriptomics
    Language English
    Dates of publication 2020-0408
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants9040470
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Dynamic formation and transcriptional regulation mediated by phytohormones during chalkiness formation in rice.

    Xie, Qin / Xu, Jinke / Huang, Ke / Su, Yi / Tong, Jianhua / Huang, Zhigang / Huang, Chao / Wei, Manlin / Lin, Wanhuang / Xiao, Langtao

    BMC plant biology

    2021  Volume 21, Issue 1, Page(s) 308

    Abstract: Background: Rice (Oryza sativa L.) Chalkiness, the opaque part in the kernel endosperm formed by loosely piled starch and protein bodies. Chalkiness is a complex quantitative trait regulated by multiple genes and various environmental factors. ... ...

    Abstract Background: Rice (Oryza sativa L.) Chalkiness, the opaque part in the kernel endosperm formed by loosely piled starch and protein bodies. Chalkiness is a complex quantitative trait regulated by multiple genes and various environmental factors. Phytohormones play important roles in the regulation of chalkiness formation but the underlying molecular mechanism is still unclear at present.
    Results: In this research, Xiangzaoxian24 (X24, pure line of indica rice with high-chalkiness) and its origin parents Xiangzaoxian11 (X11, female parent, pure line of indica rice with high-chalkiness) and Xiangzaoxian7 (X7, male parent, pure line of indica rice with low-chalkiness) were used as materials. The phenotype, physiological and biochemical traits combined with transcriptome analysis were conducted to illustrate the dynamic process and transcriptional regulation of rice chalkiness formation. Impressively, phytohormonal contents and multiple phytohormonal signals were significantly different in chalky caryopsis, suggesting the involvement of phytohormones, particularly ABA and auxin, in the regulation of rice chalkiness formation, through the interaction of multiple transcription factors and their downstream regulators.
    Conclusion: These results indicated that chalkiness formation is a dynamic process associated with multiple genes, forming a complex regulatory network in which phytohormones play important roles. These results provided informative clues for illustrating the regulatory mechanisms of chalkiness formation in rice.
    MeSH term(s) Endosperm/drug effects ; Endosperm/metabolism ; Endosperm/ultrastructure ; Gene Expression Profiling ; Gene Expression Regulation, Plant/drug effects ; Gene Ontology ; Oryza/drug effects ; Oryza/genetics ; Oryza/physiology ; Phenotype ; Plant Growth Regulators/pharmacology ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Reproducibility of Results ; Starch/metabolism ; Starch/ultrastructure ; Sucrose/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Transcription, Genetic/drug effects
    Chemical Substances Plant Growth Regulators ; Plant Proteins ; Transcription Factors ; Sucrose (57-50-1) ; Starch (9005-25-8)
    Language English
    Publishing date 2021-06-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 2059868-3
    ISSN 1471-2229 ; 1471-2229
    ISSN (online) 1471-2229
    ISSN 1471-2229
    DOI 10.1186/s12870-021-03109-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Dynamic formation and transcriptional regulation mediated by phytohormones during chalkiness formation in rice

    Xie, Qin / Xu, Jinke / Huang, Ke / Su, Yi / Tong, Jianhua / Huang, Zhigang / Huang, Chao / Wei, Manlin / Lin, Wanhuang / Xiao, Langtao

    BMC Plant Biol. 2021 Dec., v. 21, no. 1 p.308-308

    2021  

    Abstract: BACKGROUND: Rice (Oryza sativa L.) Chalkiness, the opaque part in the kernel endosperm formed by loosely piled starch and protein bodies. Chalkiness is a complex quantitative trait regulated by multiple genes and various environmental factors. ... ...

    Abstract BACKGROUND: Rice (Oryza sativa L.) Chalkiness, the opaque part in the kernel endosperm formed by loosely piled starch and protein bodies. Chalkiness is a complex quantitative trait regulated by multiple genes and various environmental factors. Phytohormones play important roles in the regulation of chalkiness formation but the underlying molecular mechanism is still unclear at present. RESULTS: In this research, Xiangzaoxian24 (X24, pure line of indica rice with high-chalkiness) and its origin parents Xiangzaoxian11 (X11, female parent, pure line of indica rice with high-chalkiness) and Xiangzaoxian7 (X7, male parent, pure line of indica rice with low-chalkiness) were used as materials. The phenotype, physiological and biochemical traits combined with transcriptome analysis were conducted to illustrate the dynamic process and transcriptional regulation of rice chalkiness formation. Impressively, phytohormonal contents and multiple phytohormonal signals were significantly different in chalky caryopsis, suggesting the involvement of phytohormones, particularly ABA and auxin, in the regulation of rice chalkiness formation, through the interaction of multiple transcription factors and their downstream regulators. CONCLUSION: These results indicated that chalkiness formation is a dynamic process associated with multiple genes, forming a complex regulatory network in which phytohormones play important roles. These results provided informative clues for illustrating the regulatory mechanisms of chalkiness formation in rice.
    Keywords Oryza sativa ; auxins ; caryopsis ; endosperm ; females ; males ; phenotype ; plant hormones ; quantitative traits ; rice ; starch ; transcription (genetics) ; transcriptomics
    Language English
    Dates of publication 2021-12
    Size p. 308.
    Publishing place BioMed Central
    Document type Article ; Online
    ZDB-ID 2059868-3
    ISSN 1471-2229
    ISSN 1471-2229
    DOI 10.1186/s12870-021-03109-z
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Detoxification of three toxins in castor meal by a novel continuous phase-transition extraction in a pilot-scale

    Liu, Guo / Liao, Mingjuan / Guo, Baoyan / Kan, Qixin / Zhou, Shuangde / Feng, Konglong / Lin, Wanhuang / Huang, Yahui / Miao, Jianyin / Cao, Yong

    Industrial crops and products. 2021 Nov. 15, v. 172

    2021  

    Abstract: Castor (Ricinus communis L.) meal, a by-product of castor oil production, is a high-quality protein source. Unfortunately, the toxic substance limits its application. Herein, a novel continuous phase transition extraction (CPTE) technology at pilot scale ...

    Abstract Castor (Ricinus communis L.) meal, a by-product of castor oil production, is a high-quality protein source. Unfortunately, the toxic substance limits its application. Herein, a novel continuous phase transition extraction (CPTE) technology at pilot scale was explored for castor meal detoxification. The detoxification conditions were optimized using response surface methodology (RSM) as: treatment time of 3 h, extraction pressure of 0.16 MPa and temperature of 84.8°C. And the removal rate of allergens, hemagglutinin, and ricin reached 79.2 %, 99.8 % and 99.0 %, respectively. The protein content of castor meal increased from 38.4% to 39.0%. The nontoxicity nature of the treated caster meal was verified via an acute toxicity tests where the mean lethal dose was >5000 mg/kg body weight. Overall, CPTE offers a novel and efficient tool for castor meal detoxification, with the superiority of non-nutrient loss, it improved the application value of castor meal and reduced the risk of environmental pollution.
    Keywords Ricinus communis ; acute toxicity ; body weight ; byproducts ; castor bean meal ; castor oil ; hemagglutinins ; lethal dose ; phase transition ; pollution ; protein content ; protein sources ; response surface methodology ; ricin ; temperature
    Language English
    Dates of publication 2021-1115
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1132158-1
    ISSN 1872-633X ; 0926-6690
    ISSN (online) 1872-633X
    ISSN 0926-6690
    DOI 10.1016/j.indcrop.2021.114076
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  6. Article: Multiple High-Affinity K

    Xie, Qin / Ma, Liying / Tan, Peng / Deng, Wentao / Huang, Chao / Liu, Deming / Lin, Wanhuang / Su, Yi

    Plants (Basel, Switzerland)

    2020  Volume 9, Issue 4

    Abstract: Potassium is an important essential element for plant growth and development. Long-term potassium deprivation can lead to a severe deficiency phenotype in plants. Interestingly, ...

    Abstract Potassium is an important essential element for plant growth and development. Long-term potassium deprivation can lead to a severe deficiency phenotype in plants. Interestingly,
    Language English
    Publishing date 2020-04-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants9040470
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Auxin Extraction and Purification Based on Recombinant Aux/IAA Proteins.

    Su, Yi / Luo, Weigui / Chen, Xiaofei / Liu, Huizhen / Hu, Yueqing / Lin, Wanhuang / Xiao, Langtao

    Biological procedures online

    2017  Volume 19, Page(s) 1

    Abstract: Background: Indole-3-acetic acid (IAA) extraction and purification are of great importance in auxin research, which is a hot topic in the plant growth and development field. Solid-phase extraction (SPE) is frequently used for IAA extraction and ... ...

    Abstract Background: Indole-3-acetic acid (IAA) extraction and purification are of great importance in auxin research, which is a hot topic in the plant growth and development field. Solid-phase extraction (SPE) is frequently used for IAA extraction and purification. However, no IAA-specific SPE columns are commercially available at the moment. Therefore, the development of IAA-specific recognition materials and IAA extraction and purification methods will help researchers meet the need for more precise analytical methods for research on phytohormones.
    Results: Since the AUXIN RESISTANT/INDOLE-3-ACETIC ACID INDUCIBLE (Aux/IAA) proteins show higher specific binding capability with auxin, recombinant IAA1, IAA7 and IAA28 proteins were used as sorbents to develop an IAA extraction and purification method. A GST tag was used to solidify the recombinant protein in a column. Aux/IAA proteins solidified in a column have successfully trapped trace IAA in aqueous solutions. The IAA7 protein showed higher IAA binding capability than the other proteins tested. In addition, expression of the IAA7 protein in
    Conclusion: This work validated the potential of Aux/IAA proteins to extract and purify IAA from crude plant extracts once we refined the techniques for these processes.
    Language English
    Publishing date 2017-01-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 2027823-8
    ISSN 1480-9222
    ISSN 1480-9222
    DOI 10.1186/s12575-016-0050-1
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  8. Article: The Copy Number Variation of

    Liu, Qing / Xu, Jinke / Zhu, Yunhua / Mo, Yuxing / Yao, Xue-Feng / Wang, Ruozhong / Ku, Wenzhen / Huang, Zhigang / Xia, Shitou / Tong, Jianhua / Huang, Chao / Su, Yi / Lin, Wanhuang / Peng, Keqin / Liu, Chun-Ming / Xiao, Langtao

    Frontiers in plant science

    2021  Volume 11, Page(s) 620282

    Abstract: Copy number variation (CNV) may have phenotypic effects by altering the expression level of the gene(s) or regulatory element(s) contained. It is believed that CNVs play pivotal roles in controlling plant architecture and other traits in plant. However, ... ...

    Abstract Copy number variation (CNV) may have phenotypic effects by altering the expression level of the gene(s) or regulatory element(s) contained. It is believed that CNVs play pivotal roles in controlling plant architecture and other traits in plant. However, the effects of CNV contributing to special traits remain largely unknown. Here we report a CNV involved in rice architecture by modulating tiller number and leaf angle. In the genome of
    Language English
    Publishing date 2021-02-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2711035-7
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2020.620282
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Genome-wide identification and characterization of auxin response factor (ARF) family genes related to flower and fruit development in papaya (Carica papaya L.).

    Liu, Kaidong / Yuan, Changchun / Li, Haili / Lin, Wanhuang / Yang, Yanjun / Shen, Chenjia / Zheng, Xiaolin

    BMC genomics

    2015  Volume 16, Page(s) 901

    Abstract: Background: Auxin and auxin signaling are involved in a series of developmental processes in plants. Auxin Response Factors (ARFs) is reported to modulate the expression of target genes by binding to auxin response elements (AuxREs) and influence the ... ...

    Abstract Background: Auxin and auxin signaling are involved in a series of developmental processes in plants. Auxin Response Factors (ARFs) is reported to modulate the expression of target genes by binding to auxin response elements (AuxREs) and influence the transcriptional activation of down-stream target genes. However, how ARF genes function in flower development and fruit ripening of papaya (Carica papaya L.) is largely unknown. In this study, a comprehensive characterization and expression profiling analysis of 11 C. papaya ARF (CpARF) genes was performed using the newly updated papaya reference genome data.
    Results: We analyzed CpARF expression patterns at different developmental stages. CpARF1, CpARF2, CpARF4, CpARF5, and CpARF10 showed the highest expression at the initial stage of flower development, but decreased during the following developmental stages. CpARF6 expression increased during the developmental process and reached its peak level at the final stage of flower development. The expression of CpARF1 increased significantly during the fruit ripening stages. Many AuxREs were included in the promoters of two ethylene signaling genes (CpETR1 and CpETR2) and three ethylene-synthesis-related genes (CpACS1, CpACS2, and CpACO1), suggesting that CpARFs might be involved in fruit ripening via the regulation of ethylene signaling.
    Conclusions: Our study provided comprehensive information on ARF family in papaya, including gene structures, chromosome locations, phylogenetic relationships, and expression patterns. The involvement of CpARF gene expression changes in flower and fruit development allowed us to understand the role of ARF-mediated auxin signaling in the maturation of reproductive organs in papaya.
    MeSH term(s) Carica/genetics ; Carica/metabolism ; Flowers/genetics ; Flowers/metabolism ; Fruit/genetics ; Fruit/metabolism ; Gene Expression Regulation, Plant ; Plant Proteins/genetics ; Plant Proteins/metabolism
    Chemical Substances Plant Proteins
    Language English
    Publishing date 2015-11-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1471-2164
    ISSN (online) 1471-2164
    DOI 10.1186/s12864-015-2182-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Model of Cation Transportation Mediated by High-Affinity Potassium Transporters (HKTs) in Higher Plants.

    Su, Yi / Luo, Weigui / Lin, Wanhuang / Ma, Liying / Kabir, Mohammed Hunayun

    Biological procedures online

    2015  Volume 17, Page(s) 1

    Abstract: Trk/Ktr/HKT transporters probably were evolved from simple K(+) channels KcsA. HKT transporters, which mediate Na(+)-uniport or Na(+)/K(+)-symport, maintain K(+)/Na(+) homeostasis and increase salinity tolerance, can be classified into three subfamilies ... ...

    Abstract Trk/Ktr/HKT transporters probably were evolved from simple K(+) channels KcsA. HKT transporters, which mediate Na(+)-uniport or Na(+)/K(+)-symport, maintain K(+)/Na(+) homeostasis and increase salinity tolerance, can be classified into three subfamilies in higher plants. In this review, we systematically analyzed the characteristics of amino acids sequences and physiological functions of HKT transporters in higher plant. Furthermore, we depicted the hypothetical models of cations selection and transportation mediated by HKT transporters according to the highly conserved structure for the goal of better understanding the cations transportation processes.
    Language English
    Publishing date 2015-01-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2027823-8
    ISSN 1480-9222
    ISSN 1480-9222
    DOI 10.1186/s12575-014-0013-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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