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Article ; Online: Release of lead, copper, zinc from the initial corrosion of brass water meter in drinking water: Influences of solution composition and electrochemical characterization.

Hsu, Ching-Hsuan / Ng, Ding-Quan / Lin, Yi-Pin

Environmental pollution (Barking, Essex : 1987)

2024 Volume 352, Page(s) 124154

Abstract: Corrosion of brass plumbing materials may lead to metal release and deteriorate the drinking water quality. In this study, the initial corrosion of brass coupon cut from commercially available water meter was investigated. High rates of Pb, Cu and Zn ... ...

Abstract	Corrosion of brass plumbing materials may lead to metal release and deteriorate the drinking water quality. In this study, the initial corrosion of brass coupon cut from commercially available water meter was investigated. High rates of Pb, Cu and Zn release from the brass coupon were found during the early stage of corrosion (0-5 d) due to general corrosion and galvanic corrosion. The corrosion current density (I
Language	English
Publishing date	2024-05-13
Publishing country	England
Document type	Journal Article
ZDB-ID	280652-6
ISSN	1873-6424 ; 0013-9327 ; 0269-7491
ISSN (online)	1873-6424
ISSN	0013-9327 ; 0269-7491
DOI	10.1016/j.envpol.2024.124154
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Article ; Online: Characterization of Fe-Containing and Pb-Containing Nanoparticles Resulting from Corrosion of Plumbing Materials in Tap Water Using a Hyphenated ATM-DMA-spICP-MS System.

Wang, Jing-Wen / Yu, Chia-Hung / Hou, Wen-Che / Hsiao, Ta-Chih / Lin, Yi-Pin

Environmental science & technology

2024 Volume 58, Issue 4, Page(s) 2038–2047

Abstract: Single-particle inductively coupled plasma mass spectrometry (spICP-MS) has been used to characterize metallic nanoparticles (NPs) assuming that all NPs are spherical and composed of pure element. However, environmental NPs generally do not meet these ... ...

Abstract	Single-particle inductively coupled plasma mass spectrometry (spICP-MS) has been used to characterize metallic nanoparticles (NPs) assuming that all NPs are spherical and composed of pure element. However, environmental NPs generally do not meet these criteria, suggesting that spICP-MS may underestimate their true sizes. This study employed a system hyphenating the atomizer (ATM), differential mobility analyzer (DMA), and spICP-MS to characterize metallic NPs in tap water. Its performance was validated by using reference Au nanoparticles (AuNPs) and Ag-shelled AuNPs. The hyphenated system can determine the actual size and metal composition of both NPs with additional heating after ATM, while stand-alone spICP-MS misidentified the Ag-shelled AuNPs as smaller individual AgNPs and AuNPs. Dissolved metal ions could introduce artifact NPs after heating but could be eliminated by centrifugation. The hyphenated system was applied to characterize Fe-containing and Pb-containing NPs resulting from the corrosion of plumbing materials in tap water. The mode sizes of Fe-containing and Pb-containing NPs were determined to be 110 and 100 nm and the particle number concentrations were determined to be 4.99 × 10
MeSH term(s)	Metal Nanoparticles/chemistry ; Gold/chemistry ; Lead ; Sanitary Engineering ; Corrosion ; Nebulizers and Vaporizers ; Particle Size ; Water
Chemical Substances	Gold (7440-57-5) ; Lead (2P299V784P) ; Water (059QF0KO0R)
Language	English
Publishing date	2024-01-19
Publishing country	United States
Document type	Journal Article
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.3c07592
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Article ; Online: Multifunctional interaction of CihC/FbpC orthologs of relapsing fever spirochetes with host-derived proteins involved in adhesion, fibrinolysis, and complement evasion.

Damm, Ann-Sophie / Reyer, Flavia / Langhoff, Luisa / Lin, Yi-Pin / Falcone, Franco Harald / Kraiczy, Peter

Frontiers in immunology

2024 Volume 15, Page(s) 1390468

Abstract: Introduction: Relapsing fever (RF) remains a neglected human disease that is caused by a number of diverse pathogenic : Methods: Binding of CihC/FbpC orthologs to different human proteins and conversion of protein-bound plasminogen to proteolytic ... ...

Abstract	Introduction: Relapsing fever (RF) remains a neglected human disease that is caused by a number of diverse pathogenic Methods: Binding of CihC/FbpC orthologs to different human proteins and conversion of protein-bound plasminogen to proteolytic active plasmin were examined by ELISA. To analyze the inhibitory capacity of CihC/FbpC orthologs on complement activation, a microtiter-based approach was performed. Finally, AlphaFold predictions were utilized to identified the complement-interacting residues. Results and discussion: Here, we elucidate the binding properties of CihC/FbpC-orthologs from distinct RF spirochetes including
MeSH term(s)	Humans ; Fibrinolysis ; Borrelia/immunology ; Borrelia/metabolism ; Relapsing Fever/microbiology ; Relapsing Fever/immunology ; Relapsing Fever/metabolism ; Plasminogen/metabolism ; Protein Binding ; Bacterial Proteins/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/immunology ; Complement Activation ; Immune Evasion ; Bacterial Adhesion ; Host-Pathogen Interactions/immunology ; Fibronectins/metabolism ; Fibrinolysin/metabolism ; Complement System Proteins/immunology ; Complement System Proteins/metabolism
Chemical Substances	Plasminogen (9001-91-6) ; Bacterial Proteins ; Fibronectins ; Fibrinolysin (EC 3.4.21.7) ; Complement System Proteins (9007-36-7)
Language	English
Publishing date	2024-04-25
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2024.1390468
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Article ; Online: Release of Particulate Lead from Four Lead Corrosion Products in Drinking Water: A Laboratory Study Coupled with Microscopic Observations and Computational Fluid Dynamics.

Peng, Yu-Cheng / Lu, Yi-Fang / Lin, Yi-Pin

Environmental science & technology

2022 Volume 56, Issue 17, Page(s) 12218–12227

Abstract: Particulate lead resulting from the detachment of lead corrosion products (LCPs) contributes significantly to lead contamination in drinking water. Since LCPs formed under different water chemistry possesses different crystal structures, their ... ...

Abstract	Particulate lead resulting from the detachment of lead corrosion products (LCPs) contributes significantly to lead contamination in drinking water. Since LCPs formed under different water chemistry possesses different crystal structures, their hydrodynamic behaviors could be significantly different in flowing water. In this study, flushing experiments and microscopic observations were employed to investigate the release of cerussite (PbCO
MeSH term(s)	Corrosion ; Drinking Water ; Hydrodynamics ; Lead ; Water Pollutants, Chemical/chemistry ; Water Supply
Chemical Substances	Drinking Water ; Water Pollutants, Chemical ; Lead (2P299V784P)
Language	English
Publishing date	2022-08-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.2c02461
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Article ; Online: Integration of in-situ chemical oxidation and permeable reactive barrier for the removal of chlorophenols by copper oxide activated peroxydisulfate.

Cho, Yi-Chin / Hsu, Chia-Chun / Lin, Yi-Pin

Journal of hazardous materials

2022 Volume 432, Page(s) 128726

Abstract: In-situ chemical oxidation (ISCO) and permeable reactive barrier (PRB) have been used in field practices for contaminated groundwater remediation. In this lab-scale study, a novel system integrating ISCO and PRB using peroxydisulfate (PDS) as the oxidant ...

Abstract	In-situ chemical oxidation (ISCO) and permeable reactive barrier (PRB) have been used in field practices for contaminated groundwater remediation. In this lab-scale study, a novel system integrating ISCO and PRB using peroxydisulfate (PDS) as the oxidant and copper oxide (CuO) as the reactive barrier material was developed for the removal of 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP). The influences of chlorophenol concentration and flow rate on the system performance were first evaluated using synthetic solutions. The removal efficiencies of target chlorophenols were greater than 90% when sufficient PDS was supplied ([PDS]/[chlorophenol]>1). It was also found that the removal efficiencies decreased with the increasing chlorophenol concentrations (10-150 μM) and flow rates (1.8-14.4 mL/min). When three real groundwaters were employed, the removal efficiencies of 2,4-DCP and 2,4,6-TCP slightly reduced to 90% and 85%, respectively. For PCP, the removal efficiency dropped to 20% in two groundwaters with relatively high levels of alkalinity. The influences of pH and TOC were found to be insignificant for the range investigated (pH 6.5-8.7 and TOC = 0.4-1.5 mgC/L). The reduced removal efficiency could be due to the formation of weaker radicals and the stronger competition between bicarbonate ions and PDS for the activation sites on the CuO surfaces.
MeSH term(s)	Chlorophenols ; Copper ; Oxidation-Reduction ; Oxides ; Water Pollutants, Chemical
Chemical Substances	Chlorophenols ; Oxides ; Water Pollutants, Chemical ; Copper (789U1901C5)
Language	English
Publishing date	2022-03-17
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1491302-1
ISSN	1873-3336 ; 0304-3894
ISSN (online)	1873-3336
ISSN	0304-3894
DOI	10.1016/j.jhazmat.2022.128726
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Article ; Online: Survey of lead concentration in tap water on a university campus.

Chang, Fu-Chun / Lin, Yi-Pin

Environmental science and pollution research international

2019 Volume 26, Issue 24, Page(s) 25275–25285

Abstract: In this study, a sampling campaign was conducted for 29 buildings on the National Taiwan University (NTU) campus where lead pipes were present in 4 buildings. Total lead, soluble lead, and water quality parameters including pH, temperature, residual free ...

Abstract	In this study, a sampling campaign was conducted for 29 buildings on the National Taiwan University (NTU) campus where lead pipes were present in 4 buildings. Total lead, soluble lead, and water quality parameters including pH, temperature, residual free chlorine, and total organic carbon were measured. Results from the first-round sequential sampling (10 × 100 mL) showed that the presence of lead pipe did not necessarily cause elevated lead levels. However, the highest lead concentration and the highest frequency of exceeding 10 μg/L were detected in a building with lead pipe. Faucet was identified to be a major lead source in at least 8 buildings. No significant correlations between lead levels and water parameters were found. The building showing the highest lead level in the first round was sampled for a second round using four different methods, namely, first draw sampling, random daytime sampling, sequential sampling (5 × 1 L), and flushing sampling. Lead levels in samples collected using the four sampling methods all exceeded 10 μg/L. The first draw sampling showed the highest lead level, followed by comparable levels for random daytime sampling and sequential sampling. Flush sampling yielded the lowest lead concentration. Particles collected from the faucet aerators in 14 buildings were also characterized for their compositions and lead contents.
MeSH term(s)	Drinking Water/chemistry ; Environmental Monitoring ; Lead/analysis ; Surveys and Questionnaires ; Taiwan ; Universities ; Water Pollutants, Chemical/analysis ; Water Quality ; Water Supply/statistics & numerical data
Chemical Substances	Drinking Water ; Water Pollutants, Chemical ; Lead (2P299V784P)
Language	English
Publishing date	2019-06-29
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-019-05771-1
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Article ; Online: Biophysical and biochemical characterization of a recombinant Lyme disease vaccine antigen, CspZ-YA.

Chen, Yi-Lin / Lee, Jungsoon / Liu, Zhuyun / Strych, Ulrich / Bottazzi, Maria Elena / Lin, Yi-Pin / Chen, Wen-Hsiang

International journal of biological macromolecules

2024 Volume 259, Issue Pt 2, Page(s) 129295

Abstract: Lyme disease, caused by Lyme Borrelia spirochetes, is the most common vector-borne illness in the United States. Despite its global significance, with an estimated 14.5 % seroprevalence, there is currently no licensed vaccine. Previously, we demonstrated ...

Abstract	Lyme disease, caused by Lyme Borrelia spirochetes, is the most common vector-borne illness in the United States. Despite its global significance, with an estimated 14.5 % seroprevalence, there is currently no licensed vaccine. Previously, we demonstrated that CspZ-YA protein conferred protection against Lyme Borrelia infection, making it a promising vaccine candidate. However, such a protein was tagged with hexahistidine, and thus not preferred for vaccine development; furthermore, the formulation to stabilize the protein was understudied. In this work, we developed a two-step purification process for tag-free E. coli-expressed recombinant CspZ-YA. We further utilized various bioassays to analyze the protein and determine the suitable buffer system for long-term storage and formulation as a vaccine immunogen. The results indicated that a buffer with a pH between 6.5 and 8.5 stabilized CspZ-YA by reducing its surface hydrophobicity and colloidal interactions. Additionally, low pH values induced a change in local spatial conformation and resulted in a decrease in α-helix content. Lastly, an optimal salinity of 22-400 mM at pH 7.5 was found to be important for its stability. Collectively, this study provides a fundamental biochemical and biophysical understanding and insights into the ideal stabilizing conditions to produce CspZ-YA recombinant protein for use in vaccine formulation and development.
MeSH term(s)	Humans ; Lyme Disease Vaccines ; Borrelia burgdorferi ; Escherichia coli/genetics ; Seroepidemiologic Studies ; Lyme Disease/prevention & control ; Bacterial Outer Membrane Proteins/chemistry
Chemical Substances	Lyme Disease Vaccines ; Bacterial Outer Membrane Proteins
Language	English
Publishing date	2024-01-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2024.129295
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Article: Editorial: Intersection of biophysical and structural approaches in vaccine design.

Ptak, Christopher P / Hsieh, Ching-Lin / Garnett, James A / Rappuoli, Rino / Lin, Yi-Pin

Frontiers in molecular biosciences

2023 Volume 10, Page(s) 1142656

Language	English
Publishing date	2023-02-14
Publishing country	Switzerland
Document type	Editorial
ZDB-ID	2814330-9
ISSN	2296-889X
ISSN	2296-889X
DOI	10.3389/fmolb.2023.1142656
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Article ; Online: Past, present, and future of Lyme disease vaccines: antigen engineering approaches and mechanistic insights.

Chen, Wen-Hsiang / Strych, Ulrich / Bottazzi, Maria Elena / Lin, Yi-Pin

Expert review of vaccines

2022 Volume 21, Issue 10, Page(s) 1405–1417

Abstract: Introduction: Transmitted by ticks, Lyme disease is the most common vector-borne disease in the Northern hemisphere. Despite the geographical expansion of human Lyme disease cases, no effective preventive strategies are currently available. Developing ... ...

Abstract	Introduction: Transmitted by ticks, Lyme disease is the most common vector-borne disease in the Northern hemisphere. Despite the geographical expansion of human Lyme disease cases, no effective preventive strategies are currently available. Developing an efficacious and safe vaccine is therefore urgently needed. Efforts have previously been taken to identify vaccine targets in the causative pathogen ( Area covered: In this review, we summarize the antigen engineering approaches that have been applied to overcome those challenges and the underlying mechanisms that can be exploited to improve both safety and efficacy of future Lyme disease vaccines. Expert opinion: Over the past two decades, several new genetically redesigned Lyme disease vaccine candidates have shown success in both preclinical and clinical settings and built a solid foundation for further development. These studies have greatly informed the protective mechanisms of reducing Lyme disease burdens and ending the endemic of this disease.
MeSH term(s)	Animals ; Borrelia burgdorferi ; Humans ; Ixodes ; Lyme Disease/epidemiology ; Lyme Disease/prevention & control ; Lyme Disease Vaccines
Chemical Substances	Lyme Disease Vaccines
Language	English
Publishing date	2022-07-22
Publishing country	England
Document type	Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2181284-6
ISSN	1744-8395 ; 1476-0584
ISSN (online)	1744-8395
ISSN	1476-0584
DOI	10.1080/14760584.2022.2102484
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Article ; Online: A two-dimensional nanoparticle characterization method combining differential mobility analyzer and single-particle inductively coupled plasma-mass spectrometry with an atomizer-enabled sample introduction (ATM-DMA-spICP-MS): Toward the analysis of heteroaggregated nanoparticles in wastewater.

Hsieh, Yi-Chin / Lin, Yi-Pin / Hsiao, Ta-Chih / Hou, Wen-Che

The Science of the total environment

2022 Volume 838, Issue Pt 3, Page(s) 156444

Abstract: Characterizing engineered nanoparticles (ENPs) in complex environmental matrices remains a challenging task. This work presents a two-dimensional size analysis method by combining differential mobility analyzer (DMA) and single-particle inductively ... ...

Abstract	Characterizing engineered nanoparticles (ENPs) in complex environmental matrices remains a challenging task. This work presents a two-dimensional size analysis method by combining differential mobility analyzer (DMA) and single-particle inductively coupled plasma-mass spectrometry (spICP-MS) with a new atomizer (ATM)-enabled sample introduction that is relatively easy to operate. The tailing of electrical mobility size distributions was solved by heating the aerosol flow, where water-shelled gold nanoparticles (AuNPs) were dehydrated, effectively eliminating the tailing. The improved method has a good sizing performance and can resolve the size fractions of mixed 30 nm and 50 nm AuNPs. It can reliably analyze 7.8 × 10
MeSH term(s)	Gold/analysis ; Mass Spectrometry/methods ; Metal Nanoparticles/chemistry ; Nebulizers and Vaporizers ; Particle Size ; Silver/chemistry ; Waste Water/analysis ; Water/analysis
Chemical Substances	Waste Water ; Water (059QF0KO0R) ; Silver (3M4G523W1G) ; Gold (7440-57-5)
Language	English
Publishing date	2022-06-02
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2022.156444
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