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  1. Article ; Online: Laboratory information management system for biosafety laboratory

    Dingzhong Sun / Linhuan Wu / Guomei Fan

    Journal of Biosafety and Biosecurity, Vol 3, Iss 1, Pp 28-

    Safety and efficiency

    2021  Volume 34

    Abstract: Laboratory information management system (LIMS) has been widely used to facilitate laboratory activities. However, the current LIMSs do not contain functions to improve the safety of laboratory work, which is the major concern of biosafety laboratories ( ... ...

    Abstract Laboratory information management system (LIMS) has been widely used to facilitate laboratory activities. However, the current LIMSs do not contain functions to improve the safety of laboratory work, which is the major concern of biosafety laboratories (BSLs). With tons of biosafety information that need to be managed and an increasing number of biosafety-related research projects under way, it is worthy of expanding the current framework of LIMS and building a system that is more suitable for BSL usage. Such a system should carefully trade off between the safety and efficiency of regular lab activities, allowing the laboratory staff to conduct their research as free as possible while ensuring their and the environment’s safety. In order to achieve this goal, the information on the research contents, laboratory personnel, experimental materials and experimental equipment need to be well collected and fully utilized by a centralized system and its databases.
    Keywords Laboratory information management system ; Biosafety ; Biological research laboratory ; Laboratory safety ; Workflow management ; Biology (General) ; QH301-705.5
    Subject code 020
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher KeAi Communications Co., Ltd.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Draft genome sequencing data of the moderately halophilic bacterium, Allobacillus halotolerans SKP2-8 from shrimp paste (ka-pi)

    Engkarat Kingkaew / Supalurk Yiamsombut / Saranporn Poothong / Wenyu Shi / Linhuan Wu / Juncai Ma / Somboon Tanasupawat

    Data in Brief, Vol 44, Iss , Pp 108549- (2022)

    2022  

    Abstract: A moderately halophilic, Gram-stain-positive, spore-forming rod-shaped bacterium, designated SKP2-8 was isolated from a traditional fermented shrimp paste (Ka-pi) collected from the market in Samut Sakhon province, Thailand. This isolate SKP2-8 was ... ...

    Abstract A moderately halophilic, Gram-stain-positive, spore-forming rod-shaped bacterium, designated SKP2-8 was isolated from a traditional fermented shrimp paste (Ka-pi) collected from the market in Samut Sakhon province, Thailand. This isolate SKP2-8 was closely related to Allobacillus halotolerans LMG 24826T with 99.56% similarity based on 16S rRNA gene sequence. The draft genome of SKP2-8 was 2.53 Mb with 2,515 coding sequences with an average G+C content of 39.5 mol%. The ANIb, ANIm, AAI and the digital DNA-DNA hybridization values of isolate SKP2-8 were 97.22%, 97.64%, 97.75% and 78.0%, respectively, compared with A. halotolerans LMG 24826T. Based on the phenotypic characteristics, DNA-DNA relatedness and phylogenomic analysis, it was identified as Allobacillus halotolerans. The genome sequence data of this isolate provide information for further analysis of the potential biotechnological use of this microorganism and guide the characterization. The draft genome was deposited at DDBJ/ EMBL/GenBank (DNA Databank of Japan/European Molecular Biology Laboratory/Genbank) (VMHF00000000).
    Keywords Allobacillus ; Draft genome ; Fermented food ; Moderately halophile ; Shrimp paste ; Computer applications to medicine. Medical informatics ; R858-859.7 ; Science (General) ; Q1-390
    Subject code 572
    Language English
    Publishing date 2022-10-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: IPGA

    Dongmei Liu / Yifei Zhang / Guomei Fan / Dingzhong Sun / Xingjiao Zhang / Zhengfei Yu / Jinfeng Wang / Linhuan Wu / Wenyu Shi / Juncai Ma

    iMeta, Vol 1, Iss 4, Pp n/a-n/a (2022)

    A handy integrated prokaryotes genome and pan‐genome analysis web service

    2022  

    Abstract: Abstract Pan‐genomics is one of the most powerful means to study genomic variation and obtain a sketch of genes within a defined clade of species. Though there are a lot of computational tools to achieve this, an integrated framework to evaluate their ... ...

    Abstract Abstract Pan‐genomics is one of the most powerful means to study genomic variation and obtain a sketch of genes within a defined clade of species. Though there are a lot of computational tools to achieve this, an integrated framework to evaluate their performance and offer the best choice to users has never been achieved. To ease the process of large‐scale prokaryotic genome analysis, we introduce Integrated Prokaryotes Genome and pan‐genome Analysis (IPGA), a one‐stop web service to analyze, compare, and visualize pan‐genome as well as individual genomes, that rids users of installing any specific tools. IPGA features a scoring system that helps users to evaluate the reliability of pan‐genome profiles generated by different packages. Thus, IPGA can help users ascertain the profiling method that is most suitable for their data set for the following analysis. In addition, IPGA integrates several downstream comparative analysis and genome analysis modules to make users achieve diverse targets.
    Keywords comparative genomics ; pan‐genome ; web service ; Computer applications to medicine. Medical informatics ; R858-859.7
    Subject code 004
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher Wiley
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Metabolite profiling of human‐originated Lachnospiraceae at the strain level

    Rashidin Abdugheni / Wen‐Zhao Wang / Yu‐Jing Wang / Meng‐Xuan Du / Feng‐Lan Liu / Nan Zhou / Cheng‐Ying Jiang / Chang‐Yu Wang / Linhuan Wu / Juncai Ma / Chang Liu / Shuang‐Jiang Liu

    iMeta, Vol 1, Iss 4, Pp n/a-n/a (2022)

    2022  

    Abstract: Abstract The human gastrointestinal (GI) tract harbors diverse microbes, and the family Lachnospiraceae is one of the most abundant and widely occurring bacterial groups in the human GI tract. Beneficial and adverse effects of the Lachnospiraceae on host ...

    Abstract Abstract The human gastrointestinal (GI) tract harbors diverse microbes, and the family Lachnospiraceae is one of the most abundant and widely occurring bacterial groups in the human GI tract. Beneficial and adverse effects of the Lachnospiraceae on host health were reported, but the diversities at species/strain levels as well as their metabolites of Lachnospiraceae have been, so far, not well documented. In the present study, we report on the collection of 77 human‐originated Lachnospiraceae species (please refer hLchsp, https://hgmb.nmdc.cn/subject/lachnospiraceae) and the in vitro metabolite profiles of 110 Lachnospiraceae strains (https://hgmb.nmdc.cn/subject/lachnospiraceae/metabolites). The Lachnospiraceae strains in hLchsp produced 242 metabolites of 17 categories. The larger categories were alcohols (89), ketones (35), pyrazines (29), short (C2–C5), and long (C > 5) chain acids (31), phenols (14), aldehydes (14), and other 30 compounds. Among them, 22 metabolites were aromatic compounds. The well‐known beneficial gut microbial metabolite, butyric acid, was generally produced by many Lachnospiraceae strains, and Agathobacter rectalis strain Lach‐101 and Coprococcus comes strain NSJ‐173 were the top 2 butyric acid producers, as 331.5 and 310.9 mg/L of butyric acids were produced in vitro, respectively. Further analysis of the publicly available cohort‐based volatile‐metabolomic data sets of human feces revealed that over 30% of the prevailing volatile metabolites were covered by Lachnospiraceae metabolites identified in this study. This study provides Lachnospiraceae strain resources together with their metabolic profiles for future studies on host–microbe interactions and developments of novel probiotics or biotherapies.
    Keywords alcohols ; aldehydes ; Blautia ; Lachnospiraceae ; metabolite profiling ; phenols ; Computer applications to medicine. Medical informatics ; R858-859.7
    Subject code 500
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher Wiley
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Enlightening the taxonomy darkness of human gut microbiomes with a cultured biobank

    Chang Liu / Meng-Xuan Du / Rexiding Abuduaini / Hai-Ying Yu / Dan-Hua Li / Yu-Jing Wang / Nan Zhou / Min-Zhi Jiang / Peng-Xia Niu / Shan-Shan Han / Hong-He Chen / Wen-Yu Shi / Linhuan Wu / Yu-Hua Xin / Juncai Ma / Yuguang Zhou / Cheng-Ying Jiang / Hong-Wei Liu / Shuang-Jiang Liu

    Microbiome, Vol 9, Iss 1, Pp 1-

    2021  Volume 29

    Abstract: Abstract Background In gut microbiome studies, the cultured gut microbial resource plays essential roles, such as helping to unravel gut microbial functions and host-microbe interactions. Although several major studies have been performed to elucidate ... ...

    Abstract Abstract Background In gut microbiome studies, the cultured gut microbial resource plays essential roles, such as helping to unravel gut microbial functions and host-microbe interactions. Although several major studies have been performed to elucidate the cultured human gut microbiota, up to 70% of the Unified Human Gastrointestinal Genome species have not been cultured to date. Large-scale gut microbial isolation and identification as well as availability to the public are imperative for gut microbial studies and further characterizing human gut microbial functions. Results In this study, we constructed a human Gut Microbial Biobank (hGMB; homepage: hgmb.nmdc.cn ) through the cultivation of 10,558 isolates from 31 sample mixtures of 239 fresh fecal samples from healthy Chinese volunteers, and deposited 1170 strains representing 400 different species in culture collections of the International Depository Authority for long-term preservation and public access worldwide. Following the rules of the International Code of Nomenclature of Prokaryotes, 102 new species were characterized and denominated, while 28 new genera and 3 new families were proposed. hGMB represented over 80% of the common and dominant human gut microbial genera and species characterized from global human gut 16S rRNA gene amplicon data (n = 11,647) and cultured 24 “most-wanted” and “medium priority” taxa proposed by the Human Microbiome Project. We in total sequenced 115 genomes representing 102 novel taxa and 13 previously known species. Further in silico analysis revealed that the newly sequenced hGMB genomes represented 22 previously uncultured species in the Unified Human Gastrointestinal Genome (UHGG) and contributed 24 representatives of potentially “dark taxa” that had not been discovered by UHGG. The nonredundant gene catalogs generated from the hGMB genomes covered over 50% of the functionally known genes (KEGG orthologs) in the largest global human gut gene catalogs and approximately 10% of the “most wanted” functionally unknown ...
    Keywords Human gut microbiomes ; Cultivation ; Biobank ; Novel taxa ; hGMB ; Microbial ecology ; QR100-130
    Subject code 590
    Language English
    Publishing date 2021-05-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Web Resources for Microbial Data

    Qinglan Sun / Li Liu / Linhuan Wu / Wei Li / Quanhe Liu / Jianyuan Zhang / Di Liu / Juncai Ma

    Genomics, Proteomics & Bioinformatics, Vol 13, Iss 1, Pp 69-

    2015  Volume 72

    Abstract: There are multitudes of web resources that are quite useful for the microbial scientific research community. Here, we provide a brief introduction on some of the most notable microbial web resources and an evaluation of them based upon our own user ... ...

    Abstract There are multitudes of web resources that are quite useful for the microbial scientific research community. Here, we provide a brief introduction on some of the most notable microbial web resources and an evaluation of them based upon our own user experience.
    Keywords Microorganism ; Web resource ; Bioinformatics tools ; Rating ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2015-02-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: Web Resources for Microbial Data

    Sun, Qinglan / Di Liu / Jianyuan Zhang / Juncai Ma / Li Liu / Linhuan Wu / Quanhe Liu / Wei Li

    Genomics, proteomics & bioinformatics. 2015 Feb., v. 13, no. 1

    2015  

    Abstract: There are multitudes of web resources that are quite useful for the microbial scientific research community. Here, we provide a brief introduction on some of the most notable microbial web resources and an evaluation of them based upon our own user ... ...

    Abstract There are multitudes of web resources that are quite useful for the microbial scientific research community. Here, we provide a brief introduction on some of the most notable microbial web resources and an evaluation of them based upon our own user experience.
    Keywords bioinformatics ; information sources ; world wide web
    Language English
    Dates of publication 2015-02
    Size p. 69-72.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 2240213-5
    ISSN 2210-3244 ; 1672-0229
    ISSN (online) 2210-3244
    ISSN 1672-0229
    DOI 10.1016/j.gpb.2015.01.008
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: The Mouse Gut Microbial Biobank expands the coverage of cultured bacteria

    Chang Liu / Nan Zhou / Meng-Xuan Du / Yu-Tong Sun / Kai Wang / Yu-Jing Wang / Dan-Hua Li / Hai-Ying Yu / Yuqin Song / Bing-Bing Bai / Yuhua Xin / Linhuan Wu / Cheng-Ying Jiang / Jie Feng / Hua Xiang / Yuguang Zhou / Juncai Ma / Jun Wang / Hong-Wei Liu /
    Shuang-Jiang Liu

    Nature Communications, Vol 11, Iss 1, Pp 1-

    2020  Volume 12

    Abstract: Here, the authors established and characterized the mouse gut microbial biobank (mGMB), which includes 244 strains and 126 species that enlarges previous mouse intestinal bacterial collections and represents a resource for studies using mouse models to ... ...

    Abstract Here, the authors established and characterized the mouse gut microbial biobank (mGMB), which includes 244 strains and 126 species that enlarges previous mouse intestinal bacterial collections and represents a resource for studies using mouse models to investigate microbiome-associated health and disease.
    Keywords Science ; Q
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: The Mouse Gut Microbial Biobank expands the coverage of cultured bacteria

    Chang Liu / Nan Zhou / Meng-Xuan Du / Yu-Tong Sun / Kai Wang / Yu-Jing Wang / Dan-Hua Li / Hai-Ying Yu / Yuqin Song / Bing-Bing Bai / Yuhua Xin / Linhuan Wu / Cheng-Ying Jiang / Jie Feng / Hua Xiang / Yuguang Zhou / Juncai Ma / Jun Wang / Hong-Wei Liu /
    Shuang-Jiang Liu

    Nature Communications, Vol 11, Iss 1, Pp 1-

    2020  Volume 12

    Abstract: Here, the authors established and characterized the mouse gut microbial biobank (mGMB), which includes 244 strains and 126 species that enlarges previous mouse intestinal bacterial collections and represents a resource for studies using mouse models to ... ...

    Abstract Here, the authors established and characterized the mouse gut microbial biobank (mGMB), which includes 244 strains and 126 species that enlarges previous mouse intestinal bacterial collections and represents a resource for studies using mouse models to investigate microbiome-associated health and disease.
    Keywords Science ; Q
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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