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  1. Article ; Online: Red blood cells: the forgotten player in hemostasis and thrombosis.

    Weisel, J W / Litvinov, R I

    Journal of thrombosis and haemostasis : JTH

    2019  Volume 17, Issue 2, Page(s) 271–282

    Abstract: New evidence has stirred up a long-standing but undeservedly forgotten interest in the role of erythrocytes, or red blood cells (RBCs), in blood clotting and its disorders. This review summarizes the most recent research that describes the involvement of ...

    Abstract New evidence has stirred up a long-standing but undeservedly forgotten interest in the role of erythrocytes, or red blood cells (RBCs), in blood clotting and its disorders. This review summarizes the most recent research that describes the involvement of RBCs in hemostasis and thrombosis. There are both quantitative and qualitative changes in RBCs that affect bleeding and thrombosis, as well as interactions of RBCs with cellular and molecular components of the hemostatic system. The changes in RBCs that affect hemostasis and thrombosis include RBC counts or hematocrit (modulating blood rheology through viscosity) and qualitative changes, such as deformability, aggregation, expression of adhesive proteins and phosphatidylserine, release of extracellular microvesicles, and hemolysis. The pathogenic mechanisms implicated in thrombotic and hemorrhagic risk include variable adherence of RBCs to the vessel wall, which depends on the functional state of RBCs and/or endothelium, modulation of platelet reactivity and platelet margination, alterations of fibrin structure and reduced susceptibility to fibrinolysis, modulation of nitric oxide availability, and the levels of von Willebrand factor and factor VIII in blood related to the ABO blood group system. RBCs are involved in platelet-driven contraction of clots and thrombi that results in formation of a tightly packed array of polyhedral erythrocytes, or polyhedrocytes, which comprises a nearly impermeable barrier that is important for hemostasis and wound healing. The revisited notion of the importance of RBCs is largely based on clinical and experimental associations between RBCs and thrombosis or bleeding, implying that RBCs are a prospective therapeutic target in hemostatic and thrombotic disorders.
    MeSH term(s) Animals ; Blood Coagulation ; Blood Platelets/metabolism ; Endothelial Cells/metabolism ; Erythrocytes/metabolism ; Hemorrhage/blood ; Hemostasis ; Humans ; Signal Transduction ; Thrombosis/blood
    Language English
    Publishing date 2019-01-07
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 2112661-6
    ISSN 1538-7836 ; 1538-7933
    ISSN (online) 1538-7836
    ISSN 1538-7933
    DOI 10.1111/jth.14360
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    Zs.MO 295: Show issues

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  2. Article ; Online: Keeping it clean: clot biofilm to wall out bacterial invasion.

    Weisel, J W / Litvinov, R I

    Journal of thrombosis and haemostasis : JTH

    2018  Volume 16, Issue 12, Page(s) 2359–2361

    MeSH term(s) Biofilms ; Fibrin ; Humans ; Thrombosis
    Chemical Substances Fibrin (9001-31-4)
    Language English
    Publishing date 2018-10-31
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 2112661-6
    ISSN 1538-7836 ; 1538-7933
    ISSN (online) 1538-7836
    ISSN 1538-7933
    DOI 10.1111/jth.14309
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  3. Article: Recommendations for the prevention and correction of thrombotic complications in COVID-19

    Safiullina, S. I. / Litvinov, R. I.

    Kazan Medical Journal

    Abstract: The new coronavirus infection caused by the SARS-CoV-2 virus (COVID-19) is characterized by a high frequency of thrombotic complications varying from venous or, more rarely, arterial thrombosis to the development of disseminated intravascular coagulation ...

    Abstract The new coronavirus infection caused by the SARS-CoV-2 virus (COVID-19) is characterized by a high frequency of thrombotic complications varying from venous or, more rarely, arterial thrombosis to the development of disseminated intravascular coagulation (DIC) and/or diffuse pulmonary vascular microthrombosis, which aggravates the disease and becomes one of the leading causes of deaths Timely and personalized anticoagulant thromboprophylaxis with low-molecular-weight heparins may prevent a severe course of the disease and improve outcomes This applies to outpatients, hospitalized patients and patients in the early post hospital period In the future, to develop comprehensive and evidence-based guidelines on the management of patients with COVID-19, it is necessary to conduct comprehensive systematic studies and comparative clinical trials of prophylaxis and treatment of hemostatic disorders in patients with COVID-19 Новая коронавирусная инфекция, вызванная вирусом SARS-CoV-2 (COVID-19), характеризуется высокой частотой тромботических осложнений - от венозных или, реже, артериальных тромбозов до развития синдрома диссеминированного внутрисосудистого свёртывания и/или диффузных воспалительных микротромбозов лёгочных сосудов, что усугубляет течение заболевания и становится одной из причин летальных исходов Как можно более ранняя и персонифицированная антикоагулянтная тромбопрофилактика низкомолекулярными гепаринами может предотвратить тяжёлое течение заболевания и улучшить его исход Это относится к амбулаторным пациентам, госпитализированным больным и пациентам в раннем постгоспитальном периоде Для выработки всесторонних научно-обоснованных рекомендаций по тактике ведения пациентов с COVID-19 в перспективе необходимо проведение комплексных систематических исследований и сравнительных испытаний по профилактике и коррекции нарушений гемостаза при COVID-19
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #859212
    Database COVID19

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  4. Article ; Online: Synthesis and antiglycation activity of 3-phenacyl substituted thiazolium salts, new analogs of Alagebrium.

    Ozerov, Alexander / Merezhkina, Darya / Zubkov, Fedor I / Litvinov, Roman / Ibragimova, Umida / Valuisky, Nikita / Borisov, Alexander / Spasov, Alexander

    Chemical biology & drug design

    2023  Volume 103, Issue 1, Page(s) e14391

    Abstract: After preliminary ab initio calculations, 3-phenacyl substituted thiazolium salts, analogs of Alagebrium, were synthesized and investigated in vitro as glycation reaction inhibitors. The most part of investigations focused on the potential of the title ... ...

    Abstract After preliminary ab initio calculations, 3-phenacyl substituted thiazolium salts, analogs of Alagebrium, were synthesized and investigated in vitro as glycation reaction inhibitors. The most part of investigations focused on the potential of the title compounds to attenuate the formation of fluorescent AGEs as well on their ability to disrupt the cross-linking formation among glycated proteins. Additionally, the capability of thiazolium salts to deglycate in the reaction of early glycation products with nitroblue tetrazolium was determined. Cytotoxicological properties of the title compounds were evaluated using LDH and MTT assays. The leader compound (3-[2-(biphenyl-4-yl)-2-oxoethyl]-1,3-thiazol-3-ium bromide) in a 50 mg/kg dose (p.o. 14 days) was further tested within an in vivo carbonyl stress model (rats, methylglyoxal 86.25 mg/kg/d, i.p., 14 days). As a result, the leader-molecule revealed a high effectiveness against all three examined mechanisms of glycation reaction inhibition in in vitro tests and was able to suppress capacity of methylglyoxal to form AGEs in vivo.
    MeSH term(s) Rats ; Animals ; Glycation End Products, Advanced/metabolism ; Pyruvaldehyde/metabolism ; Pyruvaldehyde/pharmacology ; Salts ; Thiazoles/pharmacology
    Chemical Substances Glycation End Products, Advanced ; Pyruvaldehyde (722KLD7415) ; alagebrium (DGH49JXB1F) ; Salts ; Thiazoles
    Language English
    Publishing date 2023-11-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2216600-2
    ISSN 1747-0285 ; 1747-0277
    ISSN (online) 1747-0285
    ISSN 1747-0277
    DOI 10.1111/cbdd.14391
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  5. Article ; Online: Berberis vulgaris L. extract supplementation exerts regulatory effects on the lifespan and healthspan of Drosophila through its antioxidant activity depending on the sex.

    Golubev, Denis / Platonova, Elena / Zemskaya, Nadezhda / Shevchenko, Oksana / Shaposhnikov, Mikhail / Nekrasova, Polina / Patov, Sergey / Ibragimova, Umida / Valuisky, Nikita / Borisov, Alexander / Zhukova, Xenia / Sorokina, Svetlana / Litvinov, Roman / Moskalev, Alexey

    Biogerontology

    2023  

    Abstract: Worldwide the aging population continues to increase, so the concept of healthy longevity medicine has become increasingly significant in modern society. Berberis vulgaris L. fruits serve as a functional food supplement with a high concentration of ... ...

    Abstract Worldwide the aging population continues to increase, so the concept of healthy longevity medicine has become increasingly significant in modern society. Berberis vulgaris L. fruits serve as a functional food supplement with a high concentration of bioactive compounds, which offer numerous health-promoting benefits. The goal of this study was to investigate the geroprotective effect of Berberis vulgaris L. extract. Here we show that extract of Berberis vulgaris L. can, depending on concentrate, increases lifespan up to 6%, promote healthspan (stress resistance up to 35%, locomotor activity up to 25%, integrity of the intestinal barrier up to 12%, metabolic rate up to 5%) of Drosophila melanogaster (in vitro) and exhibits antioxidant (using red blood cell tests) and antiglycation activity (using glycation of bovine serum albumin) (in vitro). In addition to this, the extract does not exhibit cytotoxic properties in vitro, unlike the well-known polyphenolic compound quercetin. qRT-PCR has revealed the involvement of metabolic, heat shock response and lipid metabolism genes in the observed effects.
    Language English
    Publishing date 2023-12-27
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2047160-9
    ISSN 1573-6768 ; 1389-5729
    ISSN (online) 1573-6768
    ISSN 1389-5729
    DOI 10.1007/s10522-023-10083-6
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  6. Article ; Online: Shear strengthens fibrin: the knob-hole interactions display 'catch-slip' kinetics.

    Litvinov, R I / Weisel, J W

    Journal of thrombosis and haemostasis : JTH

    2013  Volume 11, Issue 10, Page(s) 1933–1935

    MeSH term(s) Fibrin/chemistry ; Humans ; Kinetics ; Models, Theoretical ; Stress, Mechanical
    Chemical Substances Fibrin (9001-31-4)
    Language English
    Publishing date 2013-08-08
    Publishing country England
    Document type Letter ; Research Support, N.I.H., Extramural
    ZDB-ID 2112661-6
    ISSN 1538-7836 ; 1538-7933
    ISSN (online) 1538-7836
    ISSN 1538-7933
    DOI 10.1111/jth.12374
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  7. Article: Pathogenesis, Diagnosis, and Treatment of Hemostatic Disorders in COVID-19 Patients.

    Khalirakhmanov, A F / Idrisova, K F / Gaifullina, R F / Zinchenko, S V / Litvinov, R I / Sharafeev, A Z / Kiyasov, A P / Rizvanov, A A

    Acta naturae

    2021  Volume 13, Issue 2, Page(s) 79–84

    Abstract: The novel coronavirus infection named COVID-19 was first detected in Wuhan, China, in December 2019, and it has been responsible for significant morbidity and mortality in scores of countries. At the time this article was being written, the number of ... ...

    Abstract The novel coronavirus infection named COVID-19 was first detected in Wuhan, China, in December 2019, and it has been responsible for significant morbidity and mortality in scores of countries. At the time this article was being written, the number of infected and deceased patients continued to grow worldwide. Most patients with severe forms of the disease suffer from pneumonia and pulmonary insufficiency; in many cases, the disease is generalized and causes multiple organ failures and a dysfunction of physiological systems. One of the most serious and prognostically ominous complications from COVID-19 is coagulopathy, in particular, decompensated hypercoagulability with the risk of developing disseminated intravascular coagulation. In most cases, local and diffuse macro- and microthromboses are present, a condition which causes multiple-organ failure and thromboembolic complications. The causes and pathogenic mechanisms of coagulopathy in COVID-19 remain largely unclear, but they are associated with systemic inflammation, including the so-called cytokine storm. Despite the relatively short period of the ongoing pandemic, laboratory signs of serious hemostatic disorders have been identified and measures for specific prevention and correction of thrombosis have been developed. This review discusses the causes of COVID-19 coagulopathies and the associated complications, as well as possible approaches to their early diagnosis, prevention, and treatment.
    Language English
    Publishing date 2021-07-28
    Publishing country Russia (Federation)
    Document type Journal Article
    ISSN 2075-8251
    ISSN 2075-8251
    DOI 10.32607/actanaturae.11182
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  8. Article ; Online: An anti-DNA antibody prefers damaged dsDNA over native.

    Akberova, N I / Zhmurov, A A / Nevzorova, T A / Litvinov, R I

    Journal of biomolecular structure & dynamics

    2017  Volume 35, Issue 1, Page(s) 219–232

    Abstract: DNA-protein interactions, including DNA-antibody complexes, have both fundamental and practical significance. In particular, antibodies against double-stranded DNA play an important role in the pathogenesis of autoimmune diseases. Elucidation of ... ...

    Abstract DNA-protein interactions, including DNA-antibody complexes, have both fundamental and practical significance. In particular, antibodies against double-stranded DNA play an important role in the pathogenesis of autoimmune diseases. Elucidation of structural mechanisms of an antigen recognition and interaction of anti-DNA antibodies provides a basis for understanding the role of DNA-containing immune complexes in human pathologies and for new treatments. Here we used Molecular Dynamic simulations of bimolecular complexes of a segment of dsDNA with a monoclonal anti-DNA antibody's Fab-fragment to obtain detailed structural and physical characteristics of the dynamic intermolecular interactions. Using a computationally modified crystal structure of a Fab-DNA complex (PDB: 3VW3), we studied in silico equilibrium Molecular Dynamics of the Fab-fragment associated with two homologous dsDNA fragments, containing or not containing dimerized thymine, a product of DNA photodamage. The Fab-fragment interactions with the thymine dimer-containing DNA was thermodynamically more stable than with the native DNA. The amino acid residues constituting a paratope and the complementary nucleotide epitopes for both Fab-DNA constructs were identified. Stacking and electrostatic interactions were shown to play the main role in the antibody-dsDNA contacts, while hydrogen bonds were less significant. The aggregate of data show that the chemically modified dsDNA (containing a covalent thymine dimer) has a higher affinity toward the antibody and forms a stronger immune complex. These findings provide a mechanistic insight into formation and properties of the pathogenic anti-DNA antibodies in autoimmune diseases, such as systemic lupus erythematosus, associated with skin photosensibilization and DNA photodamage.
    MeSH term(s) Antibodies, Antinuclear/chemistry ; Antibodies, Antinuclear/metabolism ; Antigen-Antibody Complex ; DNA/chemistry ; DNA/genetics ; DNA/metabolism ; DNA Damage ; Humans ; Hydrogen Bonding ; Immunoglobulin Fab Fragments/chemistry ; Immunoglobulin Fab Fragments/metabolism ; Models, Molecular ; Molecular Conformation ; Protein Binding ; Pyrimidine Dimers/chemistry ; Thermodynamics
    Chemical Substances Antibodies, Antinuclear ; Antigen-Antibody Complex ; Immunoglobulin Fab Fragments ; Pyrimidine Dimers ; DNA (9007-49-2)
    Language English
    Publishing date 2017-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 49157-3
    ISSN 1538-0254 ; 0739-1102
    ISSN (online) 1538-0254
    ISSN 0739-1102
    DOI 10.1080/07391102.2015.1128979
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    Zs.A 2093: Show issues Location:
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  9. Article ; Online: Cytotoxic and Luminescent Properties of Novel Organotin Complexes with Chelating Antioxidant Ligand.

    Nikitin, Evgeny / Mironova, Ekaterina / Shpakovsky, Dmitry / Gracheva, Yulia / Koshelev, Daniil / Utochnikova, Valentina / Lyssenko, Konstantin / Oprunenko, Yury / Yakovlev, Dmitry / Litvinov, Roman / Seryogina, Mariya / Spasov, Alexander / Milaeva, Elena

    Molecules (Basel, Switzerland)

    2022  Volume 27, Issue 23

    Abstract: A novel polydentate chelating antioxidant ligand and series of organotin complexes on its base were synthesized and characterized by ... ...

    Abstract A novel polydentate chelating antioxidant ligand and series of organotin complexes on its base were synthesized and characterized by NMR
    Language English
    Publishing date 2022-11-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules27238359
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  10. Article: [Molecular dynamics of immune complex of photoadduct-containing DNA with Fab-Anti-DNA antibody fragment].

    Akberova, N I / Zhmurov, A A / Nevzorova, T A / Litvinov, R I

    Molekuliarnaia biologiia

    2016  Volume 50, Issue 3, Page(s) 509–519

    Abstract: Antibodies to DNA play an important role in the pathogenesis of autoimmune diseases. The elucidation of structural mechanisms of both the antigen recognition and the interaction of anti-DNA antibodies with DNA will help to understand the role of DNA- ... ...

    Abstract Antibodies to DNA play an important role in the pathogenesis of autoimmune diseases. The elucidation of structural mechanisms of both the antigen recognition and the interaction of anti-DNA antibodies with DNA will help to understand the role of DNA-containing immune complexes in various pathologies and can provide a basis for new treatment modalities. Moreover, the DNA-antibody complex is an analog of specific intracellular DNA-protein interactions. In this work, we used in silico molecular dynamic simulations of bimolecular complexes of the dsDNA segment containing the Fab fragment of an anti-DNA antibody to obtain the detailed thermodynamic and structural characteristics of dynamic intermolecular interactions. Using computationally modified crystal structure of the Fab-DNA complex (PDB ID: 3VW3), we studied the equilibrium molecular dynamics of the 64M-5 antibody Fab fragment associated with the dsDNA fragment containing the thymine dimer, the product of DNA photodamage. Amino acid residues that constitute paratopes and the complementary nucleotide epitopes for the Fab-DNA construct were identified. Stacking and electrostatic interactions were found to play the main role in mediating the most specific antibody-dsDNA contacts, while hydrogen bonds were less significant. These findings may shed light on the formation and properties of pathogenic anti-DNA antibodies in autoimmune diseases, such as systemic lupus erythematosus associated with skin photosensitivity and DNA photodamage.
    MeSH term(s) Antibodies, Antinuclear/chemistry ; Antibodies, Antinuclear/metabolism ; Antigen-Antibody Complex/chemistry ; Antigen-Antibody Complex/metabolism ; Binding Sites, Antibody ; DNA/chemistry ; DNA/metabolism ; Humans ; Hydrogen Bonding ; Immunoglobulin Fab Fragments/chemistry ; Immunoglobulin Fab Fragments/metabolism ; Light ; Molecular Dynamics Simulation ; Nucleic Acid Conformation ; Photochemical Processes ; Protein Binding ; Protein Conformation ; Pyrimidine Dimers/chemistry ; Pyrimidine Dimers/metabolism ; Static Electricity ; Thermodynamics
    Chemical Substances Antibodies, Antinuclear ; Antigen-Antibody Complex ; Immunoglobulin Fab Fragments ; Pyrimidine Dimers ; DNA (9007-49-2)
    Language Russian
    Publishing date 2016-05
    Publishing country Russia (Federation)
    Document type Journal Article
    ZDB-ID 213542-5
    ISSN 0026-8984
    ISSN 0026-8984
    DOI 10.7868/S0026898416020026
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