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Article: [Clinical evaluation of platelet-rich plasma in the treatment of early and middle stage knee osteoarthritis under 3.0T MRI T2 mapping sequence].

Wang, Z Y / Gao, W F / Shao, Y M / Gao, Y C / Xu, X S / Liu, B J / Zhang, M S

Zhonghua wai ke za zhi [Chinese journal of surgery

2023 Volume 61, Issue 2, Page(s) 138–144

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Humans ; Female ; Male ; Osteoarthritis, Knee/therapy ; Retrospective Studies ; Orthopedic Procedures ; Platelet-Rich Plasma ; Magnetic Resonance Imaging
Language	Chinese
Publishing date	2023-01-31
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	604573-x
ISSN	0529-5815
ISSN	0529-5815
DOI	10.3760/cma.j.cn112139-20220703-00296
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Article ; Online: MiR-181a promotes cell proliferation and migration through targeting KLF15 in papillary thyroid cancer.

Sun, C X / Liu, B J / Su, Y / Shi, G W / Wang, Y / Chi, J F

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

2021 Volume 24, Issue 1, Page(s) 66–75

Abstract: Introduction: Papillary thyroid cancer (PTC) is the predominant histological type of thyroid cancer, accounting for 80% of thyroid cancers. MiR-181a is a novel microRNA that is usually upregulated in multiple cancers. This study aims to explore the role ...

Abstract	Introduction: Papillary thyroid cancer (PTC) is the predominant histological type of thyroid cancer, accounting for 80% of thyroid cancers. MiR-181a is a novel microRNA that is usually upregulated in multiple cancers. This study aims to explore the role and underlying mechanism of miR-181a in PTC. Methods: CCK8 and Transwell assays were performed to evaluate cell viability and migration. The mRNA level of miR-181a and KLF15 was calculated by qRT-PCR. The protein level of E-Cadherin, N-Cadherin and GAPDH was evaluated by western blot. Dual luciferase assay was conducted to validate that miR-181a directly targeting the 3'-UTR of KLF15 mRNA in TPC-1 cells. Results: We observed that miR-181a was overexpressed and KLF15 was low expressed in PTC tissues and cell lines. Upregulation of miR-181a or downregulation of KLF15 predicted poor outcomes in PTC patients. MiR-181a improved cell growth of PTC, migration and epithelial-mesenchymal transition (EMT) in TPC-1 cells. KLF15 was a target gene of miR-181a and its expression was mediated by miR-181a. KLF15 partially reversed the facilitating effect of miR-181a on cell proliferation and migration in TPC-1 cells. Conclusion: We discovered that miR-181a served as an oncogene downregulating KLF15, thereby inhibiting cell proliferation, migration and the EMT. These findings demonstrate that miR-181a plays a significant role in PTC progression and could be a therapeutic target for PTC.
MeSH term(s)	Animals ; Cell Movement ; Cell Proliferation ; Humans ; Kruppel-Like Transcription Factors/physiology ; Mice ; MicroRNAs/physiology ; Thyroid Cancer, Papillary/pathology ; Thyroid Neoplasms/pathology
Chemical Substances	KLF15 protein, human ; Kruppel-Like Transcription Factors ; MIrn181 microRNA, human ; MicroRNAs
Language	English
Publishing date	2021-07-26
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2397359-6
ISSN	1699-3055 ; 1699-048X
ISSN (online)	1699-3055
ISSN	1699-048X
DOI	10.1007/s12094-021-02670-1
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Article: Identification of a large dataset of SNPs in hair‐fin anchovy (Setipinna tenuifilis) based on RAD‐seq

Peng, Y / Li, J. S / Zhang, K / Liu, Y. F / Li, X. P / Zhang, H / Gong, L / Liu, L. Q / Lü, Z. M / Liu, B. J

Animal genetics. 2021 June, v. 52, no. 3

2021

Abstract: Hair‐fin anchovy (Setipinna tenuifilis) is an economically important fish distributed in the West Indian Ocean and the Northwest Pacific Ocean. In this study, 154 individuals in eight populations of S. tenuifilis were sequenced and 850 million raw reads ... ...

Abstract	Hair‐fin anchovy (Setipinna tenuifilis) is an economically important fish distributed in the West Indian Ocean and the Northwest Pacific Ocean. In this study, 154 individuals in eight populations of S. tenuifilis were sequenced and 850 million raw reads were obtained using restriction site‐associated DNA sequencing (RAD‐seq). First, we identified 14 012 044 hypothetical SNP markers. A dataset of 199 903 high‐quality SNPs was collected after further screening. These SNPs have a strong ability to test the genetic diversity between the eight populations. The differentiation and genetic law between samples were explored based on SNPs in populations of S. tenuifilis. The results of this study will provide data for protecting the genetic resources of the species.
Keywords	DNA ; Setipinna ; anchovies ; animal genetics ; data collection ; genetic variation ; Indian Ocean ; Pacific Ocean
Language	English
Dates of publication	2021-06
Size	p. 371-374.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	NAL-AP-2-clean ; JOURNAL ARTICLE
ZDB-ID	632766-7
ISSN	1365-2052 ; 0268-9146 ; 0268-9154
ISSN (online)	1365-2052
ISSN	0268-9146 ; 0268-9154
DOI	10.1111/age.13062
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Article ; Online: Identification of a large dataset of SNPs in hair-fin anchovy (Setipinna tenuifilis) based on RAD-seq.

Peng, Y / Li, J S / Zhang, K / Liu, Y F / Li, X P / Zhang, H / Gong, L / Liu, L Q / Lü, Z M / Liu, B J

Animal genetics

2021 Volume 52, Issue 3, Page(s) 371–374

Abstract: Hair-fin anchovy (Setipinna tenuifilis) is an economically important fish distributed in the West Indian Ocean and the Northwest Pacific Ocean. In this study, 154 individuals in eight populations of S. tenuifilis were sequenced and 850 million raw reads ... ...

Abstract	Hair-fin anchovy (Setipinna tenuifilis) is an economically important fish distributed in the West Indian Ocean and the Northwest Pacific Ocean. In this study, 154 individuals in eight populations of S. tenuifilis were sequenced and 850 million raw reads were obtained using restriction site-associated DNA sequencing (RAD-seq). First, we identified 14 012 044 hypothetical SNP markers. A dataset of 199 903 high-quality SNPs was collected after further screening. These SNPs have a strong ability to test the genetic diversity between the eight populations. The differentiation and genetic law between samples were explored based on SNPs in populations of S. tenuifilis. The results of this study will provide data for protecting the genetic resources of the species.
MeSH term(s)	Animals ; China ; Datasets as Topic ; Fishes/genetics ; Genetics, Population ; Pacific Ocean ; Polymorphism, Single Nucleotide ; Sequence Analysis, DNA
Language	English
Publishing date	2021-04-11
Publishing country	England
Document type	Journal Article
ZDB-ID	632766-7
ISSN	1365-2052 ; 0268-9146 ; 0268-9154
ISSN (online)	1365-2052
ISSN	0268-9146 ; 0268-9154
DOI	10.1111/age.13062
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Article: In situ charge neutralization on governing particle coagulation nucleation and size distribution in macroemulsion polymerization

Liu, B. J / Meng, W. T / Zhang, M. Y / Zhang, H. X

RSC advances. 2016 Sept. 16, v. 6, no. 91

2016

Abstract: Fabricating monodispersed polymer latex particles with ∼300 nm size at high monomer concentrations by batch macroemulsion polymerization remains significantly challenging because of latex stability. In this study, we developed a novel approach based on ... ...

Abstract	Fabricating monodispersed polymer latex particles with ∼300 nm size at high monomer concentrations by batch macroemulsion polymerization remains significantly challenging because of latex stability. In this study, we developed a novel approach based on in situ charge neutralization to prepare 40 wt% solid content latex containing monodispersed sub-300 nm latex particles. The cationic initiator 2,2′-azobis(2-methylpropionamidine)dihydrochloride (AIBA) was used to induce in situ charge neutralization in the particle nucleation period and shield the negative charges of surfactant sodium dodecyl sulfate molecules to further reduce the electrostatic repulsion between primary particles, resulting in primary particle coagulation nucleation. The primary particle coagulation promoted particle number decreased to ∼1016 L−1, and the average particle size increased to ∼100 nm at a very low monomer conversion (<0.12). With increasing AIBA concentrations from 0.6 and 0.8 to 1.0 wt% (the molar ratio of AIBA/SDS is 0.64, 0.85 and 1.06, respective), the average particle size of the latex ultimately attained from 170.6 and 221.7 to 288.0 nm, respectively. Moreover, the addition of an electrolyte and copolymerization composition also governed the particle coagulation extent and affected the particle size distribution of the ultimate latex particles. To the best of our knowledge, this is the simplest, most efficient, and inexpensive approach to prepare large sized, monodispersed latex particles.
Keywords	coagulation ; copolymerization ; electrolytes ; electrostatic interactions ; latex ; neutralization ; particle size ; particle size distribution ; polymers ; sodium dodecyl sulfate ; surfactants
Language	English
Dates of publication	2016-0916
Size	p. 88701-88706.
Publishing place	The Royal Society of Chemistry
Document type	Article
ISSN	2046-2069
DOI	10.1039/c6ra18996b
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Article: Effects of replacing inorganic trace minerals with organic trace minerals on the production performance, blood profiles, and antioxidant status of broiler breeders

Wang, G / Ao, T Y / Lin, G / Liu, B J / Liu, L J / Pei, X / Tao, W J / Wang, M Q / Xiao, Z P

Poultry science. 2019 July 01, v. 98, no. 7

2019

Abstract: This study was conducted to investigate the effects of replacing inorganic trace minerals (ITMs) with organic trace minerals (OTMs) on the production performance, blood profile, and antioxidative status of broiler breeders. A total of 600 healthy broiler ...

Abstract	This study was conducted to investigate the effects of replacing inorganic trace minerals (ITMs) with organic trace minerals (OTMs) on the production performance, blood profile, and antioxidative status of broiler breeders. A total of 600 healthy broiler breeder hens, aged 40 wk, were randomly divided into 5 treatments with 4 replicates in each treatment, and fed for 10 wk. Experimental treatments were: (1) commercial levels of inorganic minerals (COM); (2) L-ITM (50% of the COM, except for Se); (3) VL-OTM (37.5% of the COM, except for Se); (4) L-OTM (equivalent to L-ITM); and (5) OTM (62.5% of the COM, except for Se). The laying rate was 9.56% higher, feed-to-egg ratio was 7.83% lower, and rate of qualified eggs was 18.33% higher (P < 0.05) for L-OTM compared to L-ITM despite equal mineral levels. The fertility with COM was significantly higher (P < 0.05) than L-ITM, VL-OTM, or L-OTM treatments. OTM and COM treatments both had increased serum LH and P4. The relatively higher mineral levels fed in COM and OTM treatments increased blood total protein (P < 0.05). In addition, activities of serum GSH-Px, Mn-SOD, and T-SOD were higher (P < 0.01), while malondialdehyde (MDA) content was lower (P < 0.05), for COM and OTM birds as compared to L-ITM and VL-OTM. The serum T-SOD of L-OTM birds was significantly higher (9.81%; P < 0.01) than that of L-ITM birds. Higher (P < 0.05) activities of liver GSH-Px and T-SOD, and lower MDA concentrations (P < 0.01) were measured in the COM, L-OTM, and OTM treatments than the L-ITM treatment. Collectively, total replacement of high levels of ITMs by lower levels of OTMs in broiler breeder diets was beneficial for productive performance under the conditions of this study.
Keywords	antioxidants ; blood proteins ; blood serum ; broiler breeders ; dietary mineral supplements ; eggs ; glutathione peroxidase ; hen feeding ; hens ; liver ; luteinizing hormone ; malondialdehyde ; mineral content ; minerals ; superoxide dismutase ; trace elements
Language	English
Dates of publication	2019-0701
Size	p. 2888-2895.
Publishing place	Poultry Science Association, Inc.
Document type	Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.3382/ps/pez035
Database	NAL-Catalogue (AGRICOLA)

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Article: [Target regulation of micro-RNA-203 to the expression of collagen type Ⅳ alpha 4 and its role in oral submucous fibrosis].

Chen, J / Liu, B J / Du, C / Cao, Q / Li, M / Feng, H

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology

2016 Volume 51, Issue 9, Page(s) 526–531

Abstract: Objective: To explore the effect of microRNA-203(miR-203) on the expression of collagen type IV alpha 4(COL4A4) and its role in oral submucous fibrosis(OSF).: Methods: It has been revealed that COL4A4 is the putative target of miR-203 by retrieving a ...

Abstract	Objective: To explore the effect of microRNA-203(miR-203) on the expression of collagen type IV alpha 4(COL4A4) and its role in oral submucous fibrosis(OSF). Methods: It has been revealed that COL4A4 is the putative target of miR-203 by retrieving a variety of bioinformatics software tools. The expression of COL4A4 protein in the indicated tissues was examined by Western blotting analysis and the potential binding sites of miR-203 and COL4A4-3'UTR was analyzed by using bioinformatics. This study integrated a fragment of COL4A4 3'UTR containing the target sequence into the pMIR-REPORT luciferase vector. Then, the COL4A4 3'UTR-luciferase and miR-203 were contransfected into 293T cells. Beta-gal was used as a control to monitor transfection efficiency. Cells were harvested and luciferase activity was measured after 24 h of transfection. Results: The relative expression mean valves of COL4A4 were 2.46 ± 2.26 in OSF and 0.70 ± 0.49 in normal control. The expression of COL4A4 in OSF was higher than normal control(P<0.05) and the difference between groups was statistically significant. The relative expression mean valves of miR-203 were 2.57 ± 2.09 in OSF and 154.07 ± 191.11 in normal control. The expression of miR-203 in OSF was lower than in normal control(P<0.01) and the difference between groups was statistically significant. Bioinformatics analysis revealed that the COL4A4 3'UTR had a conserved site (site 1) and 3 non-conserved sites(site 2, site 3, site4) complementary to the miR-203. MiR-203 could significantly inhibit site 1-luciferase reporter activity, partially inhibited site 3- and site 4-luciferase reporter activities, while no significant effect on site 3-luciferase reporter activity. Conclusions: MiR-203 was negatively correlated with the expression of COL4A4 protein in OSF. Moreover, miR-203 repressed the expression of COL4A4 by targeting 3'UTR site of COL4A4 mRNA.
Language	Chinese
Publishing date	2016-09
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1027950-7
ISSN	1002-0098
ISSN	1002-0098
DOI	10.3760/cma.j.issn.1002-0098.2016.09.004
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Article ; Online: Isolation and phylogenetic analysis of porcine deltacoronavirus from pigs with diarrhoea in Hebei province, China.

Liu, B-J / Zuo, Y-Z / Gu, W-Y / Luo, S-X / Shi, Q-K / Hou, L-S / Zhong, F / Fan, J-H

Transboundary and emerging diseases

2018 Volume 65, Issue 3, Page(s) 874–882

Abstract: Porcine deltacoronavirus (PDCoV) is a recently identified coronavirus in the genus Deltacoronavirus that can cause enteric disease with clinical signs including diarrhoea, vomiting, dehydration and mortality in neonatal piglets. Although evidence of the ... ...

Abstract	Porcine deltacoronavirus (PDCoV) is a recently identified coronavirus in the genus Deltacoronavirus that can cause enteric disease with clinical signs including diarrhoea, vomiting, dehydration and mortality in neonatal piglets. Although evidence of the prevalence of PDCoV in China is accumulating, little published information about Chinese PDCoV isolates is available. In this study, we investigated the presence of PDCoV in 49 faecal/intestinal samples from piglets with diarrhoea on different farms in Hebei province. Five samples (10.2%) were positive for PDCoV, but no coinfection of PDCoV with other enteropathogens was observed. A PDCoV strain named HB-BD was successfully isolated from the intestinal contents of a diarrhoeic piglet and serially propagated in swine testicular (ST) cells for >40 passages. The complete genome of the HB-BD strain was sequenced and analysed. Genomic analysis showed that the HB-BD strain had a closer relationship with Chinese strains than those from other countries and was grouped within the Chinese PDCoV cluster. The results of this study will be valuable for further research of PDCoV genetic evolution and development of effective diagnostic reagents, assays and potential vaccines against newly emerged PDCoV strains.
MeSH term(s)	Animals ; China/epidemiology ; Coronavirus/genetics ; Coronavirus/isolation & purification ; Coronavirus Infections/epidemiology ; Coronavirus Infections/veterinary ; Coronavirus Infections/virology ; Diarrhea/epidemiology ; Diarrhea/veterinary ; Diarrhea/virology ; Evolution, Molecular ; Feces/virology ; Genes, Viral/genetics ; Genomics ; Intestines/virology ; Phylogeny ; Swine ; Swine Diseases/epidemiology ; Swine Diseases/virology
Keywords	covid19
Language	English
Publishing date	2018-01-24
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2414822-2
ISSN	1865-1682 ; 1865-1674
ISSN (online)	1865-1682
ISSN	1865-1674
DOI	10.1111/tbed.12821
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Article: Isolation and phylogenetic analysis of porcine deltacoronavirus from pigs with diarrhoea in Hebei province, China

Liu, B.‐J / Zuo, Y.‐Z / Gu, W.‐Y / Luo, S.‐X / Shi, Q.‐K / Hou, L.‐S / Zhong, F / Fan, J.‐H

Transboundary and emerging diseases. 2018 June, v. 65, no. 3

2018

Abstract	Porcine deltacoronavirus (PDCoV) is a recently identified coronavirus in the genus Deltacoronavirus that can cause enteric disease with clinical signs including diarrhoea, vomiting, dehydration and mortality in neonatal piglets. Although evidence of the prevalence of PDCoV in China is accumulating, little published information about Chinese PDCoV isolates is available. In this study, we investigated the presence of PDCoV in 49 faecal/intestinal samples from piglets with diarrhoea on different farms in Hebei province. Five samples (10.2%) were positive for PDCoV, but no coinfection of PDCoV with other enteropathogens was observed. A PDCoV strain named HB‐BD was successfully isolated from the intestinal contents of a diarrhoeic piglet and serially propagated in swine testicular (ST) cells for >40 passages. The complete genome of the HB‐BD strain was sequenced and analysed. Genomic analysis showed that the HB‐BD strain had a closer relationship with Chinese strains than those from other countries and was grouped within the Chinese PDCoV cluster. The results of this study will be valuable for further research of PDCoV genetic evolution and development of effective diagnostic reagents, assays and potential vaccines against newly emerged PDCoV strains.
Keywords	Deltacoronavirus ; diarrhea ; emerging diseases ; enteropathogens ; farms ; genome ; genomics ; mixed infection ; mortality ; phylogeny ; piglets ; testes ; vaccines ; vomiting ; China ; covid19
Language	English
Dates of publication	2018-06
Size	p. 874-882.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2414822-2
ISSN	1865-1682 ; 1865-1674
ISSN (online)	1865-1682
ISSN	1865-1674
DOI	10.1111/tbed.12821
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Article ; Online: Effects of replacing inorganic trace minerals with organic trace minerals on the production performance, blood profiles, and antioxidant status of broiler breeders.

Wang, G / Liu, L J / Tao, W J / Xiao, Z P / Pei, X / Liu, B J / Wang, M Q / Lin, G / Ao, T Y

Poultry science

2019 Volume 98, Issue 7, Page(s) 2888–2895

Abstract	This study was conducted to investigate the effects of replacing inorganic trace minerals (ITMs) with organic trace minerals (OTMs) on the production performance, blood profile, and antioxidative status of broiler breeders. A total of 600 healthy broiler breeder hens, aged 40 wk, were randomly divided into 5 treatments with 4 replicates in each treatment, and fed for 10 wk. Experimental treatments were: (1) commercial levels of inorganic minerals (COM); (2) L-ITM (50% of the COM, except for Se); (3) VL-OTM (37.5% of the COM, except for Se); (4) L-OTM (equivalent to L-ITM); and (5) OTM (62.5% of the COM, except for Se). The laying rate was 9.56% higher, feed-to-egg ratio was 7.83% lower, and rate of qualified eggs was 18.33% higher (P < 0.05) for L-OTM compared to L-ITM despite equal mineral levels. The fertility with COM was significantly higher (P < 0.05) than L-ITM, VL-OTM, or L-OTM treatments. OTM and COM treatments both had increased serum LH and P4. The relatively higher mineral levels fed in COM and OTM treatments increased blood total protein (P < 0.05). In addition, activities of serum GSH-Px, Mn-SOD, and T-SOD were higher (P < 0.01), while malondialdehyde (MDA) content was lower (P < 0.05), for COM and OTM birds as compared to L-ITM and VL-OTM. The serum T-SOD of L-OTM birds was significantly higher (9.81%; P < 0.01) than that of L-ITM birds. Higher (P < 0.05) activities of liver GSH-Px and T-SOD, and lower MDA concentrations (P < 0.01) were measured in the COM, L-OTM, and OTM treatments than the L-ITM treatment. Collectively, total replacement of high levels of ITMs by lower levels of OTMs in broiler breeder diets was beneficial for productive performance under the conditions of this study.
MeSH term(s)	Animal Feed/analysis ; Animals ; Antioxidants/analysis ; Chickens/physiology ; Diet/veterinary ; Female ; Fertility/drug effects ; Ovum ; Random Allocation ; Trace Elements/pharmacology
Chemical Substances	Antioxidants ; Trace Elements
Language	English
Publishing date	2019-02-18
Publishing country	England
Document type	Journal Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.3382/ps/pez035
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