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  1. Article ; Online: Effectiveness of an integrative programme in reducing hypertension incidence among the population at risk for hypertension: A community-based randomized intervention study in Shanghai, China.

    Wang, Jiayun / Jiang, Qiyun / Gong, Dan / Liu, Honglian / Zhou, Peng / Zhang, Donglan / Liu, Xing / Lv, Jun / Li, Chengyue / Li, Huiqi

    Journal of global health

    2022  Volume 12, Page(s) 11013

    Abstract: Background: We aimed to evaluate the effectiveness of a community-based integrative programme in reducing hypertension incidence among populations at high risk for hypertension in Shanghai, Eastern China.: Methods: We conducted a cluster-randomized ... ...

    Abstract Background: We aimed to evaluate the effectiveness of a community-based integrative programme in reducing hypertension incidence among populations at high risk for hypertension in Shanghai, Eastern China.
    Methods: We conducted a cluster-randomized intervention trial with a total of 607 participants (intervention, n = 303; control, n = 304) between October 2019 and October 2020. A total of 605 participants (intervention, n = 302; control, n = 303) completed the follow-up survey. The intervention group received an integrative programme that included health education, physician follow-up, and self-management, while the control group received usual care only. We used questionnaires to investigate risk factors, knowledge, attitudes, and behaviours regarding hypertension prevention for all participants at baseline and follow-up. We measured the incidence of hypertension according to the predefined protocol based on the national definition during the four follow-ups (only applicable to the intervention group) and the physical examination at the end of the intervention/programme/study. The difference-in-difference (DID) effects of the intervention were estimated using Generalized Estimating Equations.
    Results: There were no significant differences in age group, gender, and educational level between intervention and control groups at baseline. The integrative programme reduced the incidence of hypertension in the intervention group compared to the control group (odds ratio (OR) = 0.27, 95% confidence interval (CI) = 0.12-0.61). The DID analysis found that the one-year intervention has improved the level of hypertension-related knowledge and attitudes regarding diagnostic criteria, complications of hypertension, and lifestyle modification (P < 0.05). The intervention was also associated with a 3.7% increase in the behaviour change rate of "not smoking" (OR = 2.50, 95% CI = 1.45-4.30) and a 34.8% increase in the rate of "monitoring blood pressure regularly" (OR = 29.61, 95% CI = 13.02-67.35).
    Conclusions: The integrative programme could reduce the risk for hypertension and improve the level of hypertension-related knowledge and attitudes, affecting the formation of healthy behaviours in high-risk populations. The community-based management for high-risk groups should be scaled up and incorporated into national hypertension control programmes, which may potentially reduce the substantial burden of hypertension and cardiovascular disease in China.
    Registration: ISRCTN registration number: ISRCTN74154693.
    Language English
    Publishing date 2022-12-17
    Publishing country Scotland
    Document type Journal Article
    ZDB-ID 2741629-X
    ISSN 2047-2986 ; 2047-2986
    ISSN (online) 2047-2986
    ISSN 2047-2986
    DOI 10.7189/jogh.12.11013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A Novel 2-dimensional Multiplex qPCR Assay for Single-Tube Detection of Nine Human Herpesviruses.

    Li, Yingxue / Wan, Zhenzhou / Zuo, Lulu / Li, Shenwei / Liu, Honglian / Ma, Yingying / Zhou, Lianqun / Jin, Xia / Li, Yuye / Zhang, Chiyu

    Virologica Sinica

    2021  Volume 36, Issue 4, Page(s) 746–754

    Abstract: Human herpesviruses are double-stranded DNA viruses that are classified into nine species. More than 90% of adults are ever infected with one or more herpesviruses. The symptoms of infection with different herpesviruses are diverse ranging from mild or ... ...

    Abstract Human herpesviruses are double-stranded DNA viruses that are classified into nine species. More than 90% of adults are ever infected with one or more herpesviruses. The symptoms of infection with different herpesviruses are diverse ranging from mild or asymptomatic infections to deadly diseases such as aggressive lymphomas and sarcomas. Timely and accurate detection of herpesvirus infection is critical for clinical management and treatment. In this study, we established a single-tube nonuple qPCR assay for detection of all nine herpesviruses using a 2-D multiplex qPCR method with a house-keeping gene as the internal control. The novel assay can detect and distinguish different herpesviruses with 30 to 300 copies per 25 µL single-tube reaction, and does not cross-react with 20 other human viruses, including DNA and RNA viruses. The robustness of the novel assay was evaluated using 170 clinical samples. The novel assay showed a high consistency (100%) with the single qPCR assay for HHVs detection. The features of simple, rapid, high sensitivity, specificity, and low cost make this assay a high potential to be widely used in clinical diagnosis and patient treatment.
    MeSH term(s) Adult ; Herpesviridae/genetics ; Herpesviridae Infections/diagnosis ; Humans ; Multiplex Polymerase Chain Reaction ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity
    Language English
    Publishing date 2021-02-26
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1011219-4
    ISSN 1995-820X ; 1000-3223 ; 1003-5125
    ISSN (online) 1995-820X
    ISSN 1000-3223 ; 1003-5125
    DOI 10.1007/s12250-021-00354-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: (cRGD)2 peptides modified nanoparticles increase tumor-targeting therapeutic effects by co-delivery of albendazole and iodine-131.

    Liu, Shengli / Liu, Honglian / Sun, Hao / Deng, Shengming / Yue, Ling / Weng, Zhen / Yang, Jianfeng / Zuo, Bin / He, Yang / Zhang, Bin

    Anti-cancer drugs

    2021  Volume 33, Issue 1, Page(s) 19–29

    Abstract: Albendazole (ABZ), a clinical antiparasitic drug, has shown potential antitumor effects in various tumors. Herein, we prepared dimeric cRGD [(cRGD)2] modified human serum albumin (HSA) nanosystem to co-delivery of albendazole (ABZ) and iodine-131 (131I) ... ...

    Abstract Albendazole (ABZ), a clinical antiparasitic drug, has shown potential antitumor effects in various tumors. Herein, we prepared dimeric cRGD [(cRGD)2] modified human serum albumin (HSA) nanosystem to co-delivery of albendazole (ABZ) and iodine-131 (131I) for chemoradiotherapy of triple-negative breast cancer (TNBC). HSA@ABZ NPs were synthesized by the self-assembly method. 131I-(cRGD)2/HSA@ABZ NPs were fabricated through covalently binding HSA@ABZ NPs with (cRGD)2 peptides, followed by chloramine T direct labeling with 131I. In vitro therapeutic effects on TNBC (MDA-MB-231 and 4T1 cells) were determined using MTT assay, crystal violet assay, wound-healing assay and western blotting analysis. In vivo treatment was performed using 4T1-bearing mice, and the tumor-targeting efficacy was assessed by gamma imaging. The distribution of NPs was quantitatively analyzed by detecting the gamma counts in tumor and main organs. The nanoparticles possessed negative charge, moderate size and good polydispersity index. Dual responding to pH and redox, the in vitro release rate of ABZ was more than 80% in 72 h. In vitro, NPs inhibited the proliferation of TNBC cells in a concentration-dependent manner and decreased cell migration. Western blotting analysis showed that the NPs, as well as free ABZ, cell-dependently induced autophagy and apoptosis by restraining or promoting the expression of p-p38 and p-JNK MAPK. In vivo, gamma imaging exhibited an earlier and denser radioactivity accumulation in tumor of 131I-(cRGD)2/HSA@ABZ NPs compared to NPs free of (cRGD)2 conjugating. Furthermore, 131I-(cRGD)2/HSA@ABZ NPs significantly suppressed tumor growth by restraining proliferation and promoting apoptosis in vivo. Our study suggested that the nanoparticles we developed enhanced tumor-targeting of ABZ and increased antitumor effects by combination of chemotherapy and radiotherapy.
    MeSH term(s) Albendazole/administration & dosage ; Albendazole/pharmacology ; Animals ; Apoptosis/drug effects ; Autophagy/drug effects ; Cell Line, Tumor ; Cell Movement/drug effects ; Chemoradiotherapy/methods ; Dose-Response Relationship, Drug ; Drug Carriers/chemistry ; Drug Liberation ; Drug Stability ; Humans ; Hydrogen-Ion Concentration ; Iodine Radioisotopes/administration & dosage ; Iodine Radioisotopes/pharmacology ; Mice, Inbred BALB C ; Nanoparticles/chemistry ; Particle Size ; Peptides, Cyclic/chemistry ; Serum Albumin ; Surface Properties ; Temperature ; Triple Negative Breast Neoplasms/pathology ; Xenograft Model Antitumor Assays ; Mice
    Chemical Substances Drug Carriers ; Iodine Radioisotopes ; Iodine-131 ; Peptides, Cyclic ; Serum Albumin ; cyclic arginine-glycine-aspartic acid peptide ; Albendazole (F4216019LN)
    Language English
    Publishing date 2021-07-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 1065301-6
    ISSN 1473-5741 ; 0959-4973
    ISSN (online) 1473-5741
    ISSN 0959-4973
    DOI 10.1097/CAD.0000000000001135
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: A Novel 2-dimensional Multiplex qPCR Assay for Single-Tube Detection of Nine Human Herpesviruses

    Li, Yingxue / Wan, Zhenzhou / Zuo, Lulu / Li, Shenwei / Liu, Honglian / Ma, Yingying / Zhou, Lianqun / Jin, Xia / Li, Yuye / Zhang, Chiyu

    Virologica Sinica. 2021 Aug., v. 36, no. 4

    2021  

    Abstract: Human herpesviruses are double-stranded DNA viruses that are classified into nine species. More than 90% of adults are ever infected with one or more herpesviruses. The symptoms of infection with different herpesviruses are diverse ranging from mild or ... ...

    Abstract Human herpesviruses are double-stranded DNA viruses that are classified into nine species. More than 90% of adults are ever infected with one or more herpesviruses. The symptoms of infection with different herpesviruses are diverse ranging from mild or asymptomatic infections to deadly diseases such as aggressive lymphomas and sarcomas. Timely and accurate detection of herpesvirus infection is critical for clinical management and treatment. In this study, we established a single-tube nonuple qPCR assay for detection of all nine herpesviruses using a 2-D multiplex qPCR method with a house-keeping gene as the internal control. The novel assay can detect and distinguish different herpesviruses with 30 to 300 copies per 25 µL single-tube reaction, and does not cross-react with 20 other human viruses, including DNA and RNA viruses. The robustness of the novel assay was evaluated using 170 clinical samples. The novel assay showed a high consistency (100%) with the single qPCR assay for HHVs detection. The features of simple, rapid, high sensitivity, specificity, and low cost make this assay a high potential to be widely used in clinical diagnosis and patient treatment.
    Keywords DNA ; Herpesviridae infections ; RNA ; essential genes ; humans ; lymphoma ; patients
    Language English
    Dates of publication 2021-08
    Size p. 746-754.
    Publishing place Springer Singapore
    Document type Article
    ZDB-ID 2425817-9
    ISSN 1995-820X ; 1674-0769
    ISSN (online) 1995-820X
    ISSN 1674-0769
    DOI 10.1007/s12250-021-00354-2
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Zoledronic acid re‑sensitises gefitinib‑resistant lung cancer cells by inhibiting the JAK/STAT3 signalling pathway and reversing epithelial‑mesenchymal transition.

    Yang, Xibiao / Gao, Yuan / Liu, Qiang / Wan, Lin / Liu, Honglian / Bian, Weiwei / Du, Yun / Huang, Chuying

    Oncology reports

    2020  Volume 45, Issue 2, Page(s) 459–468

    Abstract: Studies have shown that suppression of both the JAK/STAT3 pathway and epithelial‑mesenchymal transition (EMT) may overturn the resistance of non‑small cell lung cancer (NSCLC) cells to gefitinib. Zoledronic acid (ZA) injection is used to treat and ... ...

    Abstract Studies have shown that suppression of both the JAK/STAT3 pathway and epithelial‑mesenchymal transition (EMT) may overturn the resistance of non‑small cell lung cancer (NSCLC) cells to gefitinib. Zoledronic acid (ZA) injection is used to treat and prevent multiple forms of osteoporosis, hypercalcemia and bone metastasis‑related complications of malignancy. Clinical research has shown that ZA may exert antitumour effects and delay the progression of NSCLC. In the present study, we investigated whether ZA combined with gefitinib could re‑sensitise NSCLC cells to gefitinib in vitro and in vivo through inhibition of the JAK/STAT3 signalling pathway and EMT reversal. The results revealed that ZA potently increased the sensitivity of gefitinib‑resistant lung cancer cells to gefitinib. ZA decreased activation of JAK/STAT3 signalling and reversed EMT in the H1975 and HCC827GR cell lines. Furthermore, addition of IL‑6 to ZA‑pretreated gefitinib‑resistant cell lines abrogated the effect of ZA and restored the cellular resistance to tyrosine kinase inhibitors. Finally, ZA‑based combinatorial therapy effectively inhibited the growth of xenografts derived from gefitinib‑resistant cancer cells, which was correlated with the inhibition of the JAK/STAT3 signalling pathway and EMT reversal. In conclusion, ZA re‑sensitised gefitinib‑resistant lung cancer cells through inhibition of the JAK/STAT3 signalling pathway and EMT reversal. The combination of ZA and gefitinib may be a promising therapeutic strategy to reverse gefitinib resistance and prolong the survival of patients with NSCLC.
    MeSH term(s) Animals ; Antineoplastic Combined Chemotherapy Protocols/pharmacology ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Carcinoma, Non-Small-Cell Lung/drug therapy ; Carcinoma, Non-Small-Cell Lung/pathology ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Drug Resistance, Neoplasm/drug effects ; Drug Synergism ; Epithelial-Mesenchymal Transition/drug effects ; Female ; Gefitinib ; Humans ; Janus Kinases/metabolism ; Lung Neoplasms/drug therapy ; Lung Neoplasms/pathology ; Mice ; Phosphorylation/drug effects ; STAT3 Transcription Factor/metabolism ; Signal Transduction/drug effects ; Xenograft Model Antitumor Assays ; Zoledronic Acid/pharmacology ; Zoledronic Acid/therapeutic use
    Chemical Substances STAT3 Transcription Factor ; STAT3 protein, human ; Zoledronic Acid (6XC1PAD3KF) ; Janus Kinases (EC 2.7.10.2) ; Gefitinib (S65743JHBS)
    Language English
    Publishing date 2020-12-03
    Publishing country Greece
    Document type Journal Article
    ZDB-ID 1222484-4
    ISSN 1791-2431 ; 1021-335X
    ISSN (online) 1791-2431
    ISSN 1021-335X
    DOI 10.3892/or.2020.7881
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Radiolabeled ultra-small Fe

    Sun, Hao / Zhang, Bin / Jiang, Xinxin / Liu, Honglian / Deng, Shengming / Li, Zhen / Shi, Haibin

    Nanomedicine (London, England)

    2018  Volume 14, Issue 1, Page(s) 5–17

    Abstract: Aim: In the present study, we aimed to characterize the tumor-targeting properties of ultra-small iron oxide nanoparticles (IONPs) as multimodality imaging contrast agent.: Methods: The dimeric cRGD peptides [cyclic(Cys-Arg-Gly-Asp-dSer-Cys)-Tyr-dSer- ...

    Abstract Aim: In the present study, we aimed to characterize the tumor-targeting properties of ultra-small iron oxide nanoparticles (IONPs) as multimodality imaging contrast agent.
    Methods: The dimeric cRGD peptides [cyclic(Cys-Arg-Gly-Asp-dSer-Cys)-Tyr-dSer-Lys-Tyr-cyclic(Cys-Arg-Gly-Asp-dSer-Cys)], which specifically targeted integrin-α
    Results: The prepared IONPs demonstrated were very useful for T1/T2 and SPECT imaging of tumors in vivo, exhibiting a high tumor uptake of a clinically useful target-to-background ratio in a short time.
    Conclusion: We successfully developed a novel integrin-α
    MeSH term(s) Animals ; Cell Line, Tumor ; Cell Survival/drug effects ; Contrast Media/chemistry ; Contrast Media/metabolism ; Ferrosoferric Oxide/chemistry ; Integrin alphaVbeta3/metabolism ; Iodine Radioisotopes/chemistry ; Magnetic Resonance Imaging/methods ; Magnetite Nanoparticles/chemistry ; Mice ; Mice, Inbred BALB C ; Multimodal Imaging/methods ; Neoplasms/diagnostic imaging ; Particle Size ; Peptides, Cyclic/chemistry ; Peptides, Cyclic/metabolism ; Surface Properties ; Tissue Distribution ; Tomography, Emission-Computed, Single-Photon/methods
    Chemical Substances Contrast Media ; Integrin alphaVbeta3 ; Iodine Radioisotopes ; Magnetite Nanoparticles ; Peptides, Cyclic ; cyclic arginine-glycine-aspartic acid peptide ; Iodine-125 (GVO776611R) ; Ferrosoferric Oxide (XM0M87F357)
    Language English
    Publishing date 2018-11-19
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2277839-1
    ISSN 1748-6963 ; 1743-5889
    ISSN (online) 1748-6963
    ISSN 1743-5889
    DOI 10.2217/nnm-2018-0219
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Corrigendum to "Trend of HIV-1 drug resistance in China: A systematic review and meta-analysis of data accumulated over 17 years (2001-2017)" [EClinicalMedicine 18 (2020) 100238].

    Zuo, Lulu / Liu, Kai / Liu, Honglian / Hu, Yihong / Zhang, Zhijie / Qin, Jianru / Xu, Qinggang / Peng, Ke / Jin, Xia / Wang, Jian-Hua / Zhang, Chiyu

    EClinicalMedicine

    2021  Volume 33, Page(s) 100696

    Abstract: This corrects the article DOI: 10.1016/j.eclinm.2019.100238.]. ...

    Abstract [This corrects the article DOI: 10.1016/j.eclinm.2019.100238.].
    Language English
    Publishing date 2021-03-12
    Publishing country England
    Document type Published Erratum
    ISSN 2589-5370
    ISSN (online) 2589-5370
    DOI 10.1016/j.eclinm.2020.100696
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: 18

    Liu, Honglian / Sun, Hao / Zhang, Bin / Liu, Shengli / Deng, Shengming / Weng, Zhen / Zuo, Bin / Yang, Jianfeng / He, Yang

    Breast cancer (Tokyo, Japan)

    2019  Volume 27, Issue 3, Page(s) 372–380

    Abstract: Background: Multiple studies have indicated that albendazole (ABZ) can disrupt the microtubule in worms and have anti-tumor potential in a variety of tumors. However, the therapeutic effect of ABZ on triple-negative breast cancer (TNBC) is largely ... ...

    Abstract Background: Multiple studies have indicated that albendazole (ABZ) can disrupt the microtubule in worms and have anti-tumor potential in a variety of tumors. However, the therapeutic effect of ABZ on triple-negative breast cancer (TNBC) is largely unknown, and the therapeutic evaluation of ABZ by
    Methods: The effects of ABZ on TNBC cell lines (MDA-MB-231 cells) were investigated in vitro using MTT, wound-healing, transwell migration, flow cytometry and Western blotting analyses. In vivo treatment was conducted in an MDA-MB-231 tumor-bearing nude mouse model. Mouse body weight loss was also evaluated. PET imaging was performed before and after 3 days of treatment. Tumor tissues were harvested for immunofluorescence analysis.
    Results: ABZ treatment inhibited the proliferation and migration and triggered the apoptosis in MDA-MB-231 cells. Furthermore, Western blotting analysis showed that ABZ induced the apoptosis in MDA-MB-231 cells via GLUT1/AMPK/P53 signaling pathway. Long-term treatment studies found that the tumor volume of the treatment group was smaller compared with the control group, and the survival time was prolonged. In vivo
    Conclusions: Our study suggested that ABZ induced the apoptosis of MDA-MB-231 cells by inhibiting glucose uptake, and it could be considered as a potential drug for TNBC cells. Moreover,
    MeSH term(s) Albendazole/pharmacology ; Animals ; Apoptosis ; Cell Movement ; Cell Proliferation ; Female ; Fluorodeoxyglucose F18/metabolism ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Invasiveness ; Positron-Emission Tomography/methods ; Radiopharmaceuticals/metabolism ; Triple Negative Breast Neoplasms/drug therapy ; Triple Negative Breast Neoplasms/metabolism ; Triple Negative Breast Neoplasms/pathology ; Tubulin Modulators/pharmacology ; Tumor Cells, Cultured ; Xenograft Model Antitumor Assays
    Chemical Substances Radiopharmaceuticals ; Tubulin Modulators ; Fluorodeoxyglucose F18 (0Z5B2CJX4D) ; Albendazole (F4216019LN)
    Language English
    Publishing date 2019-11-28
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2052429-8
    ISSN 1880-4233 ; 1340-6868
    ISSN (online) 1880-4233
    ISSN 1340-6868
    DOI 10.1007/s12282-019-01027-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Trend of HIV-1 drug resistance in China: A systematic review and meta-analysis of data accumulated over 17 years (2001-2017).

    Zuo, Lulu / Liu, Kai / Liu, Honglian / Hu, Yihong / Zhang, Zhijie / Qin, Jianru / Xu, Qinggang / Peng, Ke / Jin, Xia / Wang, Jian-Hua / Zhang, Chiyu

    EClinicalMedicine

    2020  Volume 18, Page(s) 100238

    Abstract: Background: The emergence and spread of HIV-1 drug resistance may compromise HIV control globally. In response to HIV/AIDS epidemic, China launched national HIV/AIDS treatment program in 2003, and started to accumulate drug resistance data since 2001. ... ...

    Abstract Background: The emergence and spread of HIV-1 drug resistance may compromise HIV control globally. In response to HIV/AIDS epidemic, China launched national HIV/AIDS treatment program in 2003, and started to accumulate drug resistance data since 2001. In this study we aimed to assess the level, trend and distribution of HIV-1 drug resistance during a period of 17 years from 2001 to 2017, and to characterize crucial drug resistance mutations.
    Methods: We systematically reviewed 4737 studies published between January 1, 2001 and March 31, 2019 in PubMed, Embase, China National Knowledge Infrastructure (CNKI), WanFang Database, Web of Science, conference abstracts from the Chinese Medical Association and the Chinese AIDS Academic Conferences, and selected 170 studies that met our study criteria. To assess the prevalence of drug resistance in whole country or a local region, we performed pooled analyses of raw data. The transformed proportions were pooled using the inverse variance fixed effects methods or the DerSimonian-Laired random effects methods. The temporal trend of transmitted drug resistance (TDR) was determined using generalized additive model implemented in the Mgcv version 1.8 package. HIV-1 genotypic resistance was analyzed using the Stanford HIVdb algorithm.
    Findings: We assembled 218 datasets from 170 selected studies (129 in Chinese and 41 in English), covering 21,451 ART-naïve and 30,475 ART-treated individuals with HIV-1 infection. The pooled prevalence of TDR was 3.0% (95%CI: 2.8-3.2), including 0.7% (95%CI: 0.4-1.0), 1.4% (95%CI: 1.3-1.6) and 0.5% (95%CI: 0.4-0.6) for nucleoside reverse transcriptase inhibitor (NRTI), non-NRTI (NNRTI) and protease inhibitor (PI) resistance, respectively. The acquired drug resistance (ADR) prevalence was 44.7% (95%CI: 39.3-50.2), including 31.4% (95%CI: 28.2-34.6), 39.5% (95%CI: 35.6-43.5) and 1.0% (95%CI: 0.8-1.2) for NRTI, NNRTI and PI resistance, respectively. TDR and ADR prevalence had characteristic regional patterns. The worst prevalence of drug resistance occurred in Central China, and higher ADR prevalence occurred in South China than North China. TDR in whole country has risen since 2012, and this rise was driven mainly by NNRTI resistance. One NRTI-associated (M184V/I) and three NNRTI-associated (K103N/S, Y181C/I and G190A/S) mutations had high percentages in ART-naïve and ART-treated individuals, and these mutations conferred high-level resistance to 3TC, EFV and/or NVP.
    Interpretation: These findings suggest that the current available first-line ART regimens containing 3TC and/or EFV or NVP need to be revised. In addition, scale-up of multiple viral load measurements per year and drug resistance testing prior to ART initiation are recommended. Furthermore, implementation of pre-treatment education and counseling to improve patient adherence to ART is encouraged.
    Funding: This work was supported by grants from the National Natural Science Foundation of China (81672033, U1302224, and 81271888) and Open Research Fund Program of the State Key Laboratory of Virology of China (2019IOV002).
    Language English
    Publishing date 2020-01-05
    Publishing country England
    Document type Journal Article
    ISSN 2589-5370
    ISSN (online) 2589-5370
    DOI 10.1016/j.eclinm.2019.100238
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Mammalian cells use the autophagy process to restrict avian influenza virus replication.

    Liu, Siwen / Mok, Bobo Wing-Yee / Deng, Shaofeng / Liu, Honglian / Wang, Pui / Song, Wenjun / Chen, Pin / Huang, Xiaofeng / Zheng, Min / Lau, Siu-Ying / Cremin, Conor J / Tam, Chun-Yee / Li, Baiying / Jiang, Liwen / Chen, Yixin / Yuen, Kwok-Yung / Chen, Honglin

    Cell reports

    2021  Volume 35, Issue 10, Page(s) 109213

    Abstract: Host adaptive mutations in the influenza A virus (IAV) PB2 protein are critical for human infection, but their molecular action is not well understood. We observe that when IAV containing avian PB2 infects mammalian cells, viral ribonucleoprotein (vRNP) ... ...

    Abstract Host adaptive mutations in the influenza A virus (IAV) PB2 protein are critical for human infection, but their molecular action is not well understood. We observe that when IAV containing avian PB2 infects mammalian cells, viral ribonucleoprotein (vRNP) aggregates that localize to the microtubule-organizing center (MTOC) are formed. These vRNP aggregates resemble LC3B-associated autophagosome structures, with aggresome-like properties, in that they cause the re-distribution of vimentin. However, electron microscopy reveals that these aggregates represent an accumulation of autophagic vacuoles. Compared to mammalian-PB2 virus, avian-PB2 virus induces higher autophagic flux in infected cells, indicating an increased rate of autophagosomes containing avian vRNPs fusing with lysosomes. We found that p62 is essential for the formation of vRNP aggregates and that the Raptor-interacting region of p62 is required for interaction with vRNPs through the PB2 polymerase subunit. Selective autophagic sequestration during late-stage virus replication is thus an additional strategy for host restriction of avian-PB2 IAV.
    MeSH term(s) Animals ; Autophagy/genetics ; Birds ; Cell Line ; Influenza A virus/pathogenicity ; Influenza in Birds/virology ; Virus Replication/genetics
    Language English
    Publishing date 2021-06-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2649101-1
    ISSN 2211-1247 ; 2211-1247
    ISSN (online) 2211-1247
    ISSN 2211-1247
    DOI 10.1016/j.celrep.2021.109213
    Database MEDical Literature Analysis and Retrieval System OnLINE

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