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Article ; Online: Deep Learning Algorithm-Based Magnetic Resonance Imaging Feature-Guided Serum Bile Acid Profile and Perinatal Outcomes in Intrahepatic Cholestasis of Pregnancy.

Liu, Hongxue / Wang, Haidong / Zhang, Muling

Computational and mathematical methods in medicine

2022 Volume 2022, Page(s) 8081673

Abstract: This study was aimed to explore magnetic resonance imaging (MRI) based on deep learning belief network model in evaluating serum bile acid profile and adverse perinatal outcomes of intrahepatic cholestasis of pregnancy (ICP) patients. Fifty ICP pregnant ... ...

Abstract	This study was aimed to explore magnetic resonance imaging (MRI) based on deep learning belief network model in evaluating serum bile acid profile and adverse perinatal outcomes of intrahepatic cholestasis of pregnancy (ICP) patients. Fifty ICP pregnant women diagnosed in hospital were selected as the experimental group, 50 healthy pregnant women as the blank group, and 50 patients with cholelithiasis as the gallstone group. Deep learning belief network (DLBN) was built by stacking multiple restricted Boltzmann machines, which was compared with the recognition rate of convolutional neural network (CNN) and support vector machine (SVM), to determine the error rate of different recognition methods on the test set. It was found that the error rate of deep learning belief network (7.68%) was substantially lower than that of CNN (21.34%) and SVM (22.41%) (
MeSH term(s)	Bile Acids and Salts ; Cholestasis, Intrahepatic ; Deep Learning ; Female ; Gallstones ; Glycocholic Acid ; Humans ; Magnetic Resonance Imaging ; Pregnancy ; Pregnancy Complications ; Pregnancy Outcome
Chemical Substances	Bile Acids and Salts ; Glycocholic Acid (G59NX3I3RT)
Language	English
Publishing date	2022-06-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	2252430-7
ISSN	1748-6718 ; 1748-670X ; 1027-3662
ISSN (online)	1748-6718
ISSN	1748-670X ; 1027-3662
DOI	10.1155/2022/8081673
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Article ; Online: Knockdown of mediator complex subunit 27 suppresses gastric cancer cell metastasis and angiogenesis via Wnt/β-catenin pathway.

Han, Xiao / Liu, Hongxue / Tang, Xiaojun / Zhao, Yao

Tissue & cell

2022 Volume 79, Page(s) 101973

Abstract: Mediator complex (MED) contains 28 subunits, functions as a transcription machinery through interaction with RNA polymerase II and modulates gene expression involved in cell survival and growth. MED27, as an oncogene, stimulates malignant behavior of ... ...

Abstract	Mediator complex (MED) contains 28 subunits, functions as a transcription machinery through interaction with RNA polymerase II and modulates gene expression involved in cell survival and growth. MED27, as an oncogene, stimulates malignant behavior of various tumors. Role of MED27 in gastric cancer was assessed in this study. Firstly, bioinformatics analysis predicted that MED27 was elevated in gastric cancer. Gastric cancer cells also showed higher expression of MED27 than normal gastric epithelial cells. Secondly, functional assays revealed that silencing of MED27 decreased cell viability, and reduced proliferation of gastric cancer. Cell invasion and migration of gastric cancer were also inhibited by loss of MED27. Moreover, knockdown of MED27 inhibited angiogenesis of gastric cancer. Thirdly, nuclear protein of β-catenin in gastric cancer was reduced by silencing of MED27. Lastly, in vivo tumor growth of gastric cancer was suppressed by interference of MED27. In conclusion, MED27 functioned as an oncogene in gastric cancer through promoting cell metastasis and angiogenesis.
MeSH term(s)	Humans ; beta Catenin/genetics ; Stomach Neoplasms/genetics ; Wnt Signaling Pathway/genetics ; Mediator Complex/genetics
Chemical Substances	beta Catenin ; Mediator Complex
Language	English
Publishing date	2022-11-09
Publishing country	Scotland
Document type	Journal Article
ZDB-ID	204424-9
ISSN	1532-3072 ; 0040-8166
ISSN (online)	1532-3072
ISSN	0040-8166
DOI	10.1016/j.tice.2022.101973
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Article ; Online: CT Image Features under Reconstruction Algorithm in Analysis of the Effect of Probiotics Combined with Ursodeoxycholic Acid in Treatment of Intrahepatic Cholestasis of Pregnancy.

Liu, Hongxue / Wang, Haidong / Zhang, Muling

Journal of healthcare engineering

2021 Volume 2021, Page(s) 1709793

Abstract: This research was to explore the adoption value of computed tomography (CT) images based on adaptive statistical iterative reconstruction (ASIR) algorithm in the evaluation of probiotics combined with ursodeoxycholic acid in the treatment of intrahepatic ...

Abstract	This research was to explore the adoption value of computed tomography (CT) images based on adaptive statistical iterative reconstruction (ASIR) algorithm in the evaluation of probiotics combined with ursodeoxycholic acid in the treatment of intrahepatic cholestasis of pregnancy (ICP). A total of 82 patients with ICP were selected as the research subjects and they were randomly rolled into experimental group (380 mg probiotics enteric-soluble capsule twice a day, combined with 90 mg ursodeoxycholic acid soft capsule three times a day) and control group (90 mg ursodeoxycholic acid soft capsule three times a day), with 41 cases in each. The treatment course was four months. The ASIR algorithm was constructed and applied to the CT image analysis and diagnosis of ICP patients. The effects of filtering back projection (FBP) reconstruction and ASIR algorithm on CT image quality, denoising degree, and artifacts of ICP patients were compared. Moreover, blood indicator levels of ICP patients before and after treatment were assessed. The results showed that the SD values of liver and gallbladder (20.77 Hu and 27.58 Hu) in the reconstructed image of the ASIR algorithm were significantly lower than those of the FBP algorithm (40.58 Hu and 45.63 Hu) (
MeSH term(s)	Algorithms ; Cholestasis, Intrahepatic ; Humans ; Pregnancy Complications ; Probiotics ; Radiation Dosage ; Radiographic Image Interpretation, Computer-Assisted ; Tomography, X-Ray Computed ; Ursodeoxycholic Acid
Chemical Substances	Ursodeoxycholic Acid (724L30Y2QR)
Language	English
Publishing date	2021-10-31
Publishing country	England
Document type	Journal Article
ZDB-ID	2545054-2
ISSN	2040-2309 ; 2040-2295
ISSN (online)	2040-2309
ISSN	2040-2295
DOI	10.1155/2021/1709793
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Article ; Online: MicroRNA-195-5p facilitates endothelial dysfunction by inhibiting vascular endothelial growth factor A in gestational diabetes mellitus.

Zheng, Haoyu / Yu, Zhou / Wang, Hairong / Liu, Hongxue / Chen, Xiaoqin

Reproductive biology

2022 Volume 22, Issue 1, Page(s) 100605

Abstract: Gestational diabetes mellitus (GDM) is a common disorder during pregnancy associated with endothelial dysfunction in the placental vasculature. MicroRNAs (miRNAs), which are short noncoding RNAs that modulate post-transcriptional gene expression, affect ... ...

Abstract	Gestational diabetes mellitus (GDM) is a common disorder during pregnancy associated with endothelial dysfunction in the placental vasculature. MicroRNAs (miRNAs), which are short noncoding RNAs that modulate post-transcriptional gene expression, affect GDM progression. MiR-195-5p was reported to be a putative biomarker for GDM diagnosis, whose expression was markedly elevated in serum of GDM patients. Therefore, our study intended to explore whether miR-195-5p regulates endothelial cell dysfunction in GDM. Human placental microvascular endothelial cells (hPMECs) were treated with high concentration of glucose to establish an in vitro GDM model. The apoptosis, proliferation and angiogenesis of hPMECs were detected by flow cytometry analysis, CCK-8 assay and tube formation assay. The binding between vascular endothelial growth factor A (VEGFA) and miR-195-5p was verified by luciferase reporter assay. GDM mouse model was established by intraperitoneal injection of streptozocin. Cell apoptosis and the pathological changes in GDM mouse placenta tissues were evaluated by TUNEL staining and HE staining. Gene expression was detected by RT-qPCR. Protein levels were evaluated by western blotting. In this study, miR-195-5p knockdown promoted the proliferation and angiogenesis as well as inhibited the apoptosis of HG-treated hPMECs. MiR-195-5p targeted VEGFA, whose expression was downregulated in HG-treated hPMECs. VEGFA silencing antagonized the influence of miR-195-5p knockdown on the phenotypes of HG-treated hPMECs. Additionally, miR-195-5p inhibition decelerated cell apoptosis and improved pathological changes in GDM mouse placenta tissues. MiR-195-5p level was negatively correlated to VEGFA level in GDM mouse placenta tissues. Overall, miR-195-5p facilitates the endothelial cell dysfunction by inhibiting VEGFA in GDM.
MeSH term(s)	Animals ; Diabetes, Gestational/genetics ; Diabetes, Gestational/metabolism ; Diabetes, Gestational/pathology ; Endothelial Cells ; Female ; Humans ; Mice ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Placenta/metabolism ; Pregnancy ; Vascular Endothelial Growth Factor A/genetics ; Vascular Endothelial Growth Factor A/metabolism
Chemical Substances	MIRN195 microRNA, human ; MicroRNAs ; Vascular Endothelial Growth Factor A
Language	English
Publishing date	2022-01-22
Publishing country	Poland
Document type	Journal Article
ZDB-ID	2189316-0
ISSN	2300-732X ; 1642-431X
ISSN (online)	2300-732X
ISSN	1642-431X
DOI	10.1016/j.repbio.2022.100605
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Article ; Online: Corrigendum to [MicroRNA-195-5p facilitates endothelial dysfunction by inhibiting vascular endothelial growth factor A in gestational diabetes mellitus] Volume and pages of the publication: 2022 Mar;22(1):100605.

Zheng, Haoyu / Yu, Zhou / Wang, Hairong / Liu, Hongxue / Chen, Xiaoqin

Reproductive biology

2022 Volume 22, Issue 4, Page(s) 100699

Language	English
Publishing date	2022-09-28
Publishing country	Poland
Document type	Published Erratum
ZDB-ID	2189316-0
ISSN	2300-732X ; 1642-431X
ISSN (online)	2300-732X
ISSN	1642-431X
DOI	10.1016/j.repbio.2022.100699
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Article ; Online: MiRNA-621 exerts tumor suppressor function in gastric adenocarcinoma by targeting AURKA/GSK-3β pathway.

Han, Xiao / Liu, Hongxue / Tang, Xiaojun / Zhao, Yao

Acta biochimica Polonica

2021 Volume 68, Issue 1, Page(s) 91–98

Abstract: Gastric adenocarcinoma is a major challenge to human health worldwide. Abnormal expression of miR-621 was found in many types of cancer. This research aimed to investigate the effects and detailed molecular mechanisms of miR-621 on gastric adenocarcinoma ...

Abstract	Gastric adenocarcinoma is a major challenge to human health worldwide. Abnormal expression of miR-621 was found in many types of cancer. This research aimed to investigate the effects and detailed molecular mechanisms of miR-621 on gastric adenocarcinoma progression. The present study first showed that miR-621 was downregulated in gastric cancer patients, and its expression level was correlated with tumor size. MiR-621 overexpression inhibited viability, colony formation and proliferation of gastric cancer cells. AURKA was identified as a direct target of miR-621. AURKA knockdown induced decrease of p-GSK-3β/GSK-3β ratio and increase of p-β-catenin/β-catenin ratio which confirmed that AURKA positively regulated GSK-3β phosphorylation. AURKA knockdown also inhibited proliferation of gastric adenocarcinoma cells. AURKA expression was negatively correlated with miR-621 level. In addition, AURKA overexpression reversed the effect of miR-621 on the growth of cancer cells. Taken together, our results suggest that miR-621 is an important tumor suppressor in gastric cancer and could be a promising target for the cancer treatment.
MeSH term(s)	Adenocarcinoma/metabolism ; Adenocarcinoma/pathology ; Adenocarcinoma/surgery ; Aurora Kinase A/genetics ; Aurora Kinase A/metabolism ; Cell Line, Tumor ; Cell Proliferation/genetics ; Cell Survival/genetics ; Disease Progression ; Down-Regulation ; Female ; Gene Knockdown Techniques ; Genes, Tumor Suppressor ; Glycogen Synthase Kinase 3 beta/metabolism ; Humans ; Male ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Middle Aged ; Phosphorylation/genetics ; Signal Transduction/genetics ; Stomach Neoplasms/metabolism ; Stomach Neoplasms/pathology ; Stomach Neoplasms/surgery ; Transfection ; beta Catenin/metabolism
Chemical Substances	CTNNB1 protein, human ; MIRN621 microRNA, human ; MicroRNAs ; beta Catenin ; AURKA protein, human (EC 2.7.11.1) ; Aurora Kinase A (EC 2.7.11.1) ; Glycogen Synthase Kinase 3 beta (EC 2.7.11.1)
Language	English
Publishing date	2021-02-24
Publishing country	Poland
Document type	Journal Article
ZDB-ID	595762-x
ISSN	1734-154X ; 0001-527X
ISSN (online)	1734-154X
ISSN	0001-527X
DOI	10.18388/abp.2020_5452
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Zs.B 232: Show issues

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Article ; Online: MiR-125b-5p ameliorates hypoxia/reoxygenation-induced endothelial cell dysfunction and attenuates reduced uterine perfusion pressure-induced hypertension in pregnant rats via targeting BMF.

Zheng, Haoyu / Yu, Zhou / Wang, Hairong / Liu, Hongxue / Chen, Xiaoqin

Hypertension in pregnancy

2022 Volume 41, Issue 2, Page(s) 79–88

Abstract: Background and purpose: MicroRNA-125b-5p (miR-125b-5p) is downregulated in patients with gestational hypertension signs. However, the role of miR-125b-5p in pregnancy-induced hypertension (PIH) remains unknown.: Methods: The human placental ... ...

Abstract	Background and purpose: MicroRNA-125b-5p (miR-125b-5p) is downregulated in patients with gestational hypertension signs. However, the role of miR-125b-5p in pregnancy-induced hypertension (PIH) remains unknown. Methods: The human placental microvascular endothelial cells (HPMECs) have undergone hypoxia and reoxygenation (H/R) treatment to establish PIH cellular model. Rats were performed with reduced uterine perfusion pressure (RUPP) operation to establish PIH animal model. Results: MiR-125b-5p promoted viability while inhibited the apoptosis of H/R-treated HPMECs by downregulating BMF. MiR-125b-5p alleviated hypertensive symptoms and improved pregnancy outcomes in RUPP rats. Conclusion: MiR-125b-5p ameliorates H/R-induced HPMEC dysfunction and attenuates RUPP-induced hypertension in pregnant rats by downregulating BMF.
MeSH term(s)	Adaptor Proteins, Signal Transducing/genetics ; Animals ; Apoptosis ; Endothelial Cells/pathology ; Female ; Hypertension ; Hypoxia/complications ; MicroRNAs/genetics ; Perfusion ; Placenta ; Pregnancy ; Rats
Chemical Substances	Adaptor Proteins, Signal Transducing ; Bmf protein, rat ; MIRN125 microRNA, rat ; MicroRNAs
Language	English
Publishing date	2022-02-16
Publishing country	England
Document type	Journal Article
ZDB-ID	1151886-8
ISSN	1525-6065 ; 1064-1955
ISSN (online)	1525-6065
ISSN	1064-1955
DOI	10.1080/10641955.2022.2036753
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Article: Overexpressed IncRNA GATA3-AS1 in Preeclampsia and Its Effects on Trophoblast Proliferation and Migration by the miR-488-3p/ROCK1 Axis.

Zhang, Yulei / Liu, Hongxue / Shu, Xiaoming / Sun, Yanlan / Chen, Xiaoqin

Critical reviews in eukaryotic gene expression

2022 Volume 32, Issue 5, Page(s) 33–45

Abstract: Recently, dysregulation of long noncoding RNAs (lncRNAs) has been reported to be involved in the pathogenesis of preeclampsia (PE). Here, the role and molecular mechanisms of lncRNA GATA3 antisense RNA 1, GATA3-AS1 in PE were explored. The expression of ... ...

Abstract	Recently, dysregulation of long noncoding RNAs (lncRNAs) has been reported to be involved in the pathogenesis of preeclampsia (PE). Here, the role and molecular mechanisms of lncRNA GATA3 antisense RNA 1, GATA3-AS1 in PE were explored. The expression of GATA3-AS1, miR-488-3p and Rho-associated coiled-coil-containing protein kinase 1 (ROCK1) in placental tissues from patients with PE was measured by reverse transcription quantitative PCR (RT-qPCR). Proliferation, migration, invasion, and apoptosis of trophoblast cells were examined by 5-ethynyl-2'-deoxyuridine (EdU), wound healing, Transwell, and flow cytometry analyses. The subcellular localization of GATA3-AS1 in trophoblast cells was determined by fluorescent hybridization (FISH) assay. The interactions among GATA3-AS1, miR-488-3p and ROCK1 were identified by luciferase reporter and RNA pulldown assays. Our results showed that GATA3-AS1 and ROCK1 were overexpressed while miR-488-3p was downregulated in placental samples with PE. Functionally, GATA3-AS1 overexpression promoted trophoblast cell apoptosis and inhibited cell proliferation, migration, and invasion. Mechanically, GATA3-AS1 acted as a molecular sponge of miR-488-3p and miR-488-3p targeted ROCK1 in trophoblast cells. In rescue assays, ROCK1 overexpression or miR-488-3p downregulation reversed the effects of GATA3-AS1 silencing on trophoblast cell phenotypes. GATA3-AS1 is overexpressed in PE and promotes PE progression by the miR-488-3p/ROCK1 axis.
MeSH term(s)	Cell Line, Tumor ; Cell Movement/genetics ; Cell Proliferation/genetics ; Female ; GATA3 Transcription Factor/genetics ; GATA3 Transcription Factor/metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; MicroRNAs ; Placenta/metabolism ; Pre-Eclampsia/genetics ; Pre-Eclampsia/pathology ; Pregnancy ; RNA, Long Noncoding/genetics ; Trophoblasts/metabolism ; rho-Associated Kinases/genetics ; rho-Associated Kinases/metabolism
Chemical Substances	GATA3 Transcription Factor ; GATA3 protein, human ; MIRN488 microRNA, human ; MicroRNAs ; RNA, Long Noncoding ; ROCK1 protein, human (EC 2.7.11.1) ; rho-Associated Kinases (EC 2.7.11.1)
Language	English
Publishing date	2022-08-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	1071345-1
ISSN	1045-4403
ISSN	1045-4403
DOI	10.1615/CritRevEukaryotGeneExpr.2021040776
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Article ; Online: Circ_0007121 Facilitates Trophoblastic Cell Proliferation, Migration, and Invasion via the Regulation of the miR-421/ZEB1 Axis in Preeclampsia.

Zhou, Fenmei / Liu, Hongxue / Zhang, Ruirui / Sun, Yanlan

Reproductive sciences (Thousand Oaks, Calif.)

2021 Volume 29, Issue 1, Page(s) 100–109

Abstract: Noncoding circular RNAs (circRNAs) have participated in the progression of preeclampsia (PE) via inhibiting microRNAs (miRNAs) to regulate gene expression. This study was designed to explore the miRNA/mRNA mechanism of hsa_circ_0007121 (circ_0007121) in ... ...

Abstract	Noncoding circular RNAs (circRNAs) have participated in the progression of preeclampsia (PE) via inhibiting microRNAs (miRNAs) to regulate gene expression. This study was designed to explore the miRNA/mRNA mechanism of hsa_circ_0007121 (circ_0007121) in PE. The expression detection of circ_0007121, microRNA-421 (miR-421), and zinc finger E-box binding homeobox 1 (ZEB1) was performed by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation was analyzed by Cell Counting Kit-8 (CCK-8) assay. Transwell assay was used to determine cell migration and invasion. Cell apoptosis was evaluated using flow cytometry. The protein levels of epithelial-mesenchymal transition (EMT) markers and ZEB1 were measured via western blot. The interaction between miR-421 and circ_0007121 or ZEB1 was validated by dual-luciferase reporter assay. The expression detection indicated that circ_0007121 was downregulated in PE patients and the clinical data revealed that circ_0007121 was related to PE. The upregulation of circ_0007121 promoted cell proliferation, migration, invasion, and EMT in trophoblastic cells. Furthermore, circ_0007121 was identified as a sponge of miR-421 and the function of circ_0007121 was dependent on the sponge effect on miR-421. Moreover, ZEB1 was a target of miR-421 and circ_0007121/miR-421 axis could regulate the expression of ZEB1. In addition, miR-421 overexpression repressed trophoblastic cell behaviors through downregulating the ZEB1 level. Altogether, circ_0007121 contributed to the development of trophoblastic cells by regulating the miR-421/ZEB1 axis.
MeSH term(s)	Cell Line ; Cell Movement/physiology ; Cell Proliferation/physiology ; Epithelial-Mesenchymal Transition/genetics ; Female ; Gene Expression Regulation ; Humans ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Pre-Eclampsia/genetics ; Pre-Eclampsia/metabolism ; Pregnancy ; RNA, Circular/genetics ; RNA, Circular/metabolism ; Signal Transduction/physiology ; Trophoblasts/metabolism ; Zinc Finger E-box-Binding Homeobox 1/genetics ; Zinc Finger E-box-Binding Homeobox 1/metabolism
Chemical Substances	MicroRNAs ; RNA, Circular ; ZEB1 protein, human ; Zinc Finger E-box-Binding Homeobox 1
Language	English
Publishing date	2021-08-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	2276411-2
ISSN	1933-7205 ; 1933-7191
ISSN (online)	1933-7205
ISSN	1933-7191
DOI	10.1007/s43032-021-00713-0
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Article: MicroRNA-195-5p facilitates endothelial dysfunction by inhibiting vascular endothelial growth factor A in gestational diabetes mellitus

Zheng, Haoyu / Yu, Zhou / Wang, Hairong / Liu, Hongxue / Chen, Xiaoqin

Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn Reproductive Biology. 2022 Mar., v. 22, no. 1

2022

Abstract	Gestational diabetes mellitus (GDM) is a common disorder during pregnancy associated with endothelial dysfunction in the placental vasculature. MicroRNAs (miRNAs), which are short noncoding RNAs that modulate post-transcriptional gene expression, affect GDM progression. MiR-195-5p was reported to be a putative biomarker for GDM diagnosis, whose expression was markedly elevated in serum of GDM patients. Therefore, our study intended to explore whether miR-195-5p regulates endothelial cell dysfunction in GDM. Human placental microvascular endothelial cells (hPMECs) were treated with high concentration of glucose to establish an in vitro GDM model. The apoptosis, proliferation and angiogenesis of hPMECs were detected by flow cytometry analysis, CCK-8 assay and tube formation assay. The binding between vascular endothelial growth factor A (VEGFA) and miR-195-5p was verified by luciferase reporter assay. GDM mouse model was established by intraperitoneal injection of streptozocin. Cell apoptosis and the pathological changes in GDM mouse placenta tissues were evaluated by TUNEL staining and HE staining. Gene expression was detected by RT-qPCR. Protein levels were evaluated by western blotting. In this study, miR-195-5p knockdown promoted the proliferation and angiogenesis as well as inhibited the apoptosis of HG-treated hPMECs. MiR-195-5p targeted VEGFA, whose expression was downregulated in HG-treated hPMECs. VEGFA silencing antagonized the influence of miR-195-5p knockdown on the phenotypes of HG-treated hPMECs. Additionally, miR-195-5p inhibition decelerated cell apoptosis and improved pathological changes in GDM mouse placenta tissues. MiR-195-5p level was negatively correlated to VEGFA level in GDM mouse placenta tissues. Overall, miR-195-5p facilitates the endothelial cell dysfunction by inhibiting VEGFA in GDM.
Keywords	angiogenesis ; apoptosis ; biomarkers ; blood serum ; endothelial cells ; flow cytometry ; gene expression ; gestational diabetes ; glucose ; humans ; intraperitoneal injection ; luciferase ; mice ; microRNA ; placenta ; pregnancy ; streptozotocin ; vascular endothelial growth factor A
Language	English
Dates of publication	2022-03
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2189316-0
ISSN	1642-431X
ISSN	1642-431X
DOI	10.1016/j.repbio.2022.100605
Database	NAL-Catalogue (AGRICOLA)

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