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  1. Article ; Online: Construction and drug resistance evaluation of cell strains which stably express FIP1L1-PDGFRA protein and its mutants

    LIU Jingwen, SONG Haoxin, ZHU Lei

    Jichu yixue yu linchuang, Vol 44, Iss 1, Pp 63-

    2024  Volume 68

    Abstract: Objective To construct mouse BaF3-FIP1L1-PDGFRA (F/P), BaF3-F/P-T674I and BAF3-F/P-D842V pre-B cell strains which stably express F/P fusion protein and its T674I and D842V mutants in order to evaluate their activity by checking their responses to ... ...

    Abstract Objective To construct mouse BaF3-FIP1L1-PDGFRA (F/P), BaF3-F/P-T674I and BAF3-F/P-D842V pre-B cell strains which stably express F/P fusion protein and its T674I and D842V mutants in order to evaluate their activity by checking their responses to tyrosine kinase inhibitors (TKIs). Methods Lentivirus infected technique was used to transfect the target gene into BaF3 cells. RT-qPCR was used to detect mRNA expression, and CCK-8 method was used to detect the inhibitory effect of TKIs on the proliferation of stable cell strains. Results The constructed BaF3-F/P, BaF3-F/P-T674I and BAF3-F/P-D842V cell strains all transcripted FIP1L1 and PDGFRA mRNA. They exhibited malignant phenotypic characteristics of proliferation independent of IL-3 and sensitivity to corresponding TKIs. Conclusions The pre-B-cell strains stably expressing F/P and its T674I and D842V mutants are successfully constructed, which provide a good cell model for the development of compounds targeting at those molecules.
    Keywords fip1l1-pdgfra fusion gene|gene mutation|lentiviral vector|baf3 cell|chronic eosinophilic leukemia ; Medicine ; R
    Subject code 571
    Language Chinese
    Publishing date 2024-01-01T00:00:00Z
    Publisher Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Race/Ethnicity, Nativity, and Gender Disparities in Mental Health Trajectories from Mid- to Later-Life: A Life Course-Intersectional Approach.

    Liu, Jingwen / Lin, Zhiyong

    Journal of racial and ethnic health disparities

    2023  

    Abstract: Background: Numerous studies have highlighted mental health disparities based on race/ethnicity, nativity, and gender across different life stages. However, few have investigated how the intersectionality of these factors influences mental health ... ...

    Abstract Background: Numerous studies have highlighted mental health disparities based on race/ethnicity, nativity, and gender across different life stages. However, few have investigated how the intersectionality of these factors influences mental health trajectories during midlife to late life. This study fills this gap by adopting a life course-intersectional approach, viewing mental health trajectories as dynamic processes shaped by the combined influences of race/ethnicity, nativity, and gender. It explores social, psychological, and physiological pathways contributing to these disparities.
    Design: Using data from the Health and Retirement Study (2006-2018; N = 38,049 observations) and growth curve models, this study examines how intra-individual trends in depressive symptoms (measured as CES-D scale, 07) are influenced by the intersection of race/ethnicity, nativity, and gender. It also investigates the impact of objective and subjective social isolation and physical health on group disparities in mental health trajectories.
    Results: The findings reveal that, during mid- to early late-life, most Black and Hispanic Americans experience higher levels of depressive symptoms compared to their White counterparts (disparities ranging from 0.184 to 0.463 for men and 0.117 to 0.439 for women). However, this disadvantage diminishes for US-born Hispanic men and US-born Black women (0.014-0.031 faster decrease rates compared to US-born White), while it intensifies for Hispanic immigrants (0.017-0.018 slower decrease rates compared to US-born White) in advanced ages. Mediation analysis demonstrates that both social isolation and physical health contribute to these disparities, with physical health explaining a larger portion, particularly in differences between immigrant Hispanic women and US-born Whites.
    Conclusion: This study underscores the importance of a life course-intersectional approach in understanding mental health disparities. It emphasizes the need for improved social welfare systems and community-level interventions targeting the specific challenges faced by older Hispanic immigrants, especially women who encounter multiple forms of oppression.
    Language English
    Publishing date 2023-09-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2760524-3
    ISSN 2196-8837 ; 2197-3792
    ISSN (online) 2196-8837
    ISSN 2197-3792
    DOI 10.1007/s40615-023-01808-x
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  3. Article ; Online: The piRNA size matters.

    Liu, Jingwen / Lu, Falong

    National science review

    2023  Volume 11, Issue 2, Page(s) nwad297

    Language English
    Publishing date 2023-11-22
    Publishing country China
    Document type Journal Article
    ZDB-ID 2745465-4
    ISSN 2053-714X ; 2053-714X
    ISSN (online) 2053-714X
    ISSN 2053-714X
    DOI 10.1093/nsr/nwad297
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  4. Article ; Online: Visualization of in vivo cell-cell contact in the present and in the past.

    Liu, Jingwen / Lu, Falong

    Science China. Life sciences

    2023  Volume 66, Issue 4, Page(s) 889–891

    Language English
    Publishing date 2023-01-06
    Publishing country China
    Document type Journal Article
    ZDB-ID 2546732-3
    ISSN 1869-1889 ; 1674-7305
    ISSN (online) 1869-1889
    ISSN 1674-7305
    DOI 10.1007/s11427-022-2262-y
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  5. Article: Case Report: An extremely rare penile shrapnel injury.

    Pan, Renbing / Liu, Jingwen / Wan, Lijun / Zhu, Jianyong

    Frontiers in pediatrics

    2024  Volume 11, Page(s) 1281413

    Abstract: Penile shrapnel injuries are an exceedingly rare occurrence and a medical emergency. Herein, we present a case of penile shrapnel wounds in an adolescent male and discuss the management and complications associated with penetrating injuries to penile. We ...

    Abstract Penile shrapnel injuries are an exceedingly rare occurrence and a medical emergency. Herein, we present a case of penile shrapnel wounds in an adolescent male and discuss the management and complications associated with penetrating injuries to penile. We reported that an 18-year-old Chinese armed police soldier underwent debridement, shrapnel removal and suturing under spinal anesthesia. Six days postoperatively, he was discharged from the hospital smoothly. The patient reported normal erectile function and urination following discharge. With a follow-up of three months, the patient exhibited no symptoms of dysuria or erectile dysfunction. It is explicitly stated that prompt surgery intervention described in this report resulted in optimal prognosis. Penile shrapnel injury is a rare phenomenon typically associated with emergency drill and military training involving explosive shells. With regard to penetrating penile injury, timely surgical exploration is essential because it avoids penile plaque formation, penile fibrosis and angulation, and accelerates the return to erectile and urination function.
    Language English
    Publishing date 2024-01-08
    Publishing country Switzerland
    Document type Case Reports
    ZDB-ID 2711999-3
    ISSN 2296-2360
    ISSN 2296-2360
    DOI 10.3389/fped.2023.1281413
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  6. Article ; Online: Transsynaptic degeneration of ventral horn motor neurons exists but plays a minor role in lower motor system dysfunction in acute ischemic rats.

    Zhang, Lei / Liu, Jingwen / Liu, Mingsheng

    PloS one

    2024  Volume 19, Issue 4, Page(s) e0298006

    Abstract: Background: As a leading cause of mortality and long-term disability, acute ischemic stroke can produce far-reaching pathophysiological consequences. Accumulating evidence has demonstrated abnormalities in the lower motor system following stroke, while ... ...

    Abstract Background: As a leading cause of mortality and long-term disability, acute ischemic stroke can produce far-reaching pathophysiological consequences. Accumulating evidence has demonstrated abnormalities in the lower motor system following stroke, while the existence of Transsynaptic degeneration of contralateral spinal cord ventral horn (VH) neurons is still debated.
    Methods: Using a rat model of acute ischemic stroke, we analyzed spinal cord VH neuron counts contralaterally and ipsilaterally after stroke with immunofluorescence staining. Furthermore, we estimated the overall lower motor unit abnormalities after stroke by simultaneously measuring the modified neurological severity score (mNSS), compound muscle action potential (CMAP) amplitude, repetitive nerve stimulation (RNS), spinal cord VH neuron counts, and the corresponding muscle fiber morphology. The activation status of microglia and extracellular signal-regulated kinase 1/2 (ERK 1/2) in the spinal cord VH was also assessed.
    Results: At 7 days after stroke, the contralateral CMAP amplitudes declined to a nadir indicating lower motor function damage, and significant muscle disuse atrophy was observed on the same side; meanwhile, the VH neurons remained intact. At 14 days after focal stroke, lower motor function recovered with alleviated muscle disuse atrophy, while transsynaptic degeneration occurred on the contralateral side with elevated activation of ERK 1/2, along with the occurrence of neurogenic muscle atrophy. No apparent decrement of CMAP amplitude was observed with RNS during the whole experimental process.
    Conclusions: This study offered an overview of changes in the lower motor system in experimental ischemic rats. We demonstrated that transsynaptic degeneration of contralateral VH neurons occurred when lower motor function significantly recovered, which indicated the minor role of transsynaptic degeneration in lower motor dysfunction during the acute and subacute phases of focal ischemic stroke.
    MeSH term(s) Animals ; Rats ; Male ; Anterior Horn Cells/pathology ; Rats, Sprague-Dawley ; Synapses/pathology ; Synapses/physiology ; Disease Models, Animal ; Nerve Degeneration/pathology ; Nerve Degeneration/physiopathology ; Motor Neurons/pathology ; Motor Neurons/physiology ; Brain Ischemia/pathology ; Brain Ischemia/physiopathology ; Microglia/pathology ; Action Potentials/physiology
    Language English
    Publishing date 2024-04-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0298006
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  7. Article ; Online: A preliminary investigation of epigenome-wide DNA methylation and temperament during infancy.

    Leri, John / Liu, Jingwen / Kelly, Maria / Kertes, Darlene A

    Developmental psychobiology

    2024  Volume 66, Issue 3, Page(s) e22475

    Abstract: This study provides preliminary evidence for an epigenetic architecture of infant temperament. At 12 months of age, blood was collected and assayed for DNA methylation and maternally reported infant temperament was assessed using the Infant Behavior ... ...

    Abstract This study provides preliminary evidence for an epigenetic architecture of infant temperament. At 12 months of age, blood was collected and assayed for DNA methylation and maternally reported infant temperament was assessed using the Infant Behavior Questionnaire in 67 mother-infant dyads. Epigenome-wide analyses showed that the higher order temperament dimensions Surgency and Negative Affect were associated with DNA methylation. The epigenetic signatures of Surgency and Negative Affect were situated at genes involved in synaptic signaling and plasticity. Although replication is required, these results are consistent with a biologically based model of temperament, create new avenues for hypothesis-driven research into epigenetic pathways that underlie individual differences in temperament, and demonstrate that infant temperament has a widespread epigenetic signature in the methylome.
    MeSH term(s) Infant ; Humans ; DNA Methylation/genetics ; Epigenome ; Temperament ; Epigenomics ; Individuality
    Language English
    Publishing date 2024-03-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 4107-5
    ISSN 1098-2302 ; 0012-1630
    ISSN (online) 1098-2302
    ISSN 0012-1630
    DOI 10.1002/dev.22475
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    In stock of ZB MED Cologne/Königswinter

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  8. Article: cxcl12a

    Wei, Zhaohui / Hong, Qiang / Ding, Zijiao / Liu, Jingwen

    Frontiers in cell and developmental biology

    2023  Volume 11, Page(s) 1243265

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2023-10-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2023.1243265
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  9. Article ; Online: How China's digital technology development affects the environmental costs related to global value chains? Evidence from regional manufacturing sectors.

    Zhang, Tianding / Wei, Lixia / Liu, Jingwen

    The Science of the total environment

    2023  Volume 886, Page(s) 163978

    Abstract: China needs to address the environmental costs to participate in global value chains (GVCs) more sustainably. Digitalization presents a potential solution to overcome information barriers and improve energy efficiency in global production networks. ... ...

    Abstract China needs to address the environmental costs to participate in global value chains (GVCs) more sustainably. Digitalization presents a potential solution to overcome information barriers and improve energy efficiency in global production networks. However, the impact of digital technology on GVC-related carbon intensity has not been systematically studied. This paper utilizes embedded multi-regional input-output (EMRIO) tables to analyze the relationship between digital technology and GVC-related carbon intensity in China's 28 regions and 11 manufacturing sectors. The study calculates three indexes: digital technology development, the openness degree of digital technology, and the carbon intensity related to GVCs for the years 2002, 2007, 2010, and 2012. Through a fixed-effect model, the paper examines how digital technology can reduce domestic embodied carbon intensity related to GVCs in China. The findings indicate that: (1) digital technology development has a negative impact on GVC-related domestic embodied carbon intensity; (2) digital technology development indirectly reduces domestic embodied carbon intensity by decreasing energy consumption intensity and increasing foreign services' input intensity; (3) enhancing the openness of digital technology in China can strengthen the reduction effect of digital technology development on the environmental cost related to GVCs.
    MeSH term(s) Industrial Development ; Digital Technology ; Carbon Dioxide/analysis ; China ; Carbon ; Economic Development
    Chemical Substances Carbon Dioxide (142M471B3J) ; Carbon (7440-44-0)
    Language English
    Publishing date 2023-05-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2023.163978
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  10. Article ; Online: Overview of m

    Zhang, Leyu / Wang, Xi / Zhao, Wei / Liu, Jingwen

    Journal of cancer research and clinical oncology

    2023  Volume 149, Issue 9, Page(s) 6769–6784

    Abstract: ... ...

    Abstract N
    MeSH term(s) Humans ; Neoplasms/genetics ; RNA/metabolism ; RNA Splicing/genetics ; RNA, Circular/genetics ; RNA, Circular/metabolism
    Chemical Substances RNA (63231-63-0) ; RNA, Circular ; N-methyladenosine (CLE6G00625)
    Language English
    Publishing date 2023-02-18
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 134792-5
    ISSN 1432-1335 ; 0171-5216 ; 0084-5353 ; 0943-9382
    ISSN (online) 1432-1335
    ISSN 0171-5216 ; 0084-5353 ; 0943-9382
    DOI 10.1007/s00432-023-04610-8
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