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  1. Book: Rheumatoid arthritis

    Liu, Shuang

    methods and protocols

    (Methods in molecular biology ; 2766 ; Springer protocols)

    2024  

    Author's details edited by Shuang Liu
    Series title Methods in molecular biology ; 2766
    Springer protocols
    Collection
    Language English
    Size xi, 354 Seiten, Illustrationen
    Edition Second edition
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    HBZ-ID HT030650319
    ISBN 9781071636817 ; 9781071636824 ; 1071636812 ; 1071636820
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2024 A 231 available for loan (reading room) Lesesaal EG

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  2. Article ; Online: Electrophysiological Methods to Measure Ca

    Liu, Shuang

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2766, Page(s) 191–198

    Abstract: To achieve the most accurate assessment of functional ... ...

    Abstract To achieve the most accurate assessment of functional Ca
    MeSH term(s) Humans ; Calcium ; Environment ; Ion Transport ; Laboratories ; Patch-Clamp Techniques
    Chemical Substances Calcium (SY7Q814VUP)
    Language English
    Publishing date 2024-01-25
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3682-4_21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Release of Antibodies and Cytokines from B Cells.

    Liu, Shuang

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2766, Page(s) 233–240

    Abstract: Autoreactive B cells play a critical role in rheumatoid arthritis (RA). These cells differentiate into long-living memory B cells and autoantibody-producing plasma cells, and also present autoantigens to T cells to amplify misdirected immune responses. ... ...

    Abstract Autoreactive B cells play a critical role in rheumatoid arthritis (RA). These cells differentiate into long-living memory B cells and autoantibody-producing plasma cells, and also present autoantigens to T cells to amplify misdirected immune responses. The therapeutic benefit of B-cell-deleting depleting therapies suggests that B cells are emerging as important factors in the pathogenesis of RA. Aiming at evaluation of the function of B cells, which are usually derived from peripheral blood of RA patients and healthy donors, it is possible to conduct a series of experiments, such as in vitro assessment of antibody production and BCR-mediated cytokine release. These techniques can also be applied for in vivo application.
    MeSH term(s) Humans ; B-Lymphocytes ; Plasma Cells ; Autoantibodies ; Memory B Cells ; Arthritis, Rheumatoid ; Cytokines
    Chemical Substances Autoantibodies ; Cytokines
    Language English
    Publishing date 2024-01-25
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3682-4_24
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Mesenchymal Stem Cell Engineering.

    Liu, Shuang

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2766, Page(s) 169–174

    Abstract: Mesenchymal stem cells (MSC) are multipotent stem cells that display the capacity to generate the tissue in which they reside. MSC have been used as progenitor cells to engineer cartilage implants that can be used to repair chondral and osteochondral ... ...

    Abstract Mesenchymal stem cells (MSC) are multipotent stem cells that display the capacity to generate the tissue in which they reside. MSC have been used as progenitor cells to engineer cartilage implants that can be used to repair chondral and osteochondral lesions, or as trophic producers of bioactive factors to initiate endogenous regenerative activities in the arthritic joint. Targeted gene therapy might further enhance the capacity of MSC for chondrogenesis. By using a clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins genomic manipulation technique, target gene-modified MSC would be a promising therapeutic option for regeneration of diseased joints in the treatment of RA.
    MeSH term(s) Mesenchymal Stem Cells ; Multipotent Stem Cells ; Stem Cells ; CRISPR-Associated Proteins ; Chondrogenesis
    Chemical Substances CRISPR-Associated Proteins
    Language English
    Publishing date 2024-01-25
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3682-4_18
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Single-Cell Ca

    Liu, Shuang

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2766, Page(s) 183–190

    Abstract: In rheumatological studies, visualization of ... ...

    Abstract In rheumatological studies, visualization of Ca
    MeSH term(s) Diagnostic Imaging ; Data Analysis ; Dissection ; Signal Transduction
    Language English
    Publishing date 2024-01-25
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3682-4_20
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Scaffolded Chondrogenic Spheroid-Engrafted Model.

    Liu, Shuang

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2766, Page(s) 17–24

    Abstract: Therapeutic approaches using mesenchymal stem cells (MSCs) for a cartilage regeneration strategy are based on their multipotent differentiation for skeletal regeneration. With the utilization of allergenic neutralized type I atelocollagen during the pre- ... ...

    Abstract Therapeutic approaches using mesenchymal stem cells (MSCs) for a cartilage regeneration strategy are based on their multipotent differentiation for skeletal regeneration. With the utilization of allergenic neutralized type I atelocollagen during the pre-formation of chondrogenic MSC spheroids, cellular condensation and chondrogenic differentiation can be easily achieved. It also benefits the recruitment of host MSCs, which differentiate into chondrocyte-like cells after implantation into the experiment model. Using pre-formed chondrogenic MSC spheroids, the efficacy of anti-rheumatoid agents for cartilage repair can be screened on a large scale ex vivo. Furthermore, atelocollagen-scaffolded chondrogenic spheroids can be utilized for in vivo transplantation into a humanized xenografted arthritis model. Thus, the ability of cartilage self-repair can be qualitatively and quantitatively evaluated.
    MeSH term(s) Animals ; Cell Differentiation ; Chondrogenesis ; Disease Models, Animal ; Embryo Implantation ; Mesenchymal Stem Cells
    Language English
    Publishing date 2024-01-25
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3682-4_3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Bioinformatics analysis identifies GLUD1 as a prognostic indicator for clear cell renal cell carcinoma.

    Liu, Shuang

    European journal of medical research

    2024  Volume 29, Issue 1, Page(s) 70

    Abstract: Background: Renal cell carcinoma (RCC) is a common primary tumor of the kidney and is divided into three major subtypes, of which clear cell renal cell carcinoma (ccRCC) has the highest incidence. Glutamate dehydrogenase 1 (GLUD1) encodes glutamate ... ...

    Abstract Background: Renal cell carcinoma (RCC) is a common primary tumor of the kidney and is divided into three major subtypes, of which clear cell renal cell carcinoma (ccRCC) has the highest incidence. Glutamate dehydrogenase 1 (GLUD1) encodes glutamate dehydrogenase 1, which catalyzes the oxidative deamination of glutamate.
    Methods: We analyzed TCGA data using R language software and used multiple online databases to explore the relationship of GLUD1 with signaling pathways and drug sensitivity as well as GLUD1 protein expression and methylation.
    Results: The results showed that GLUD1 mRNA expression was reduced in tumor tissues and correlated with the progression of ccRCC. Univariate and multivariate Cox analysis showed that GLUD1 could be used as a prognostic marker for ccRCC. GLUD1 expression in ccRCC was associated with immune cells infiltration and multiple classical signaling pathways. In addition, GLUD1 mRNA expression was related to drug sensitivity.
    Conclusions: These findings provide new ideas for finding new prognostic molecular markers and therapeutic targets for ccRCC.
    MeSH term(s) Humans ; Carcinoma, Renal Cell/genetics ; Carcinoma, Renal Cell/pathology ; Prognosis ; Kidney Neoplasms/genetics ; Kidney Neoplasms/metabolism ; Kidney Neoplasms/pathology ; Glutamate Dehydrogenase ; Computational Biology ; RNA, Messenger/metabolism
    Chemical Substances Glutamate Dehydrogenase (EC 1.4.1.2) ; RNA, Messenger ; GLUD1 protein, human (EC 1.4.1.3)
    Language English
    Publishing date 2024-01-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 1329381-3
    ISSN 2047-783X ; 0949-2321
    ISSN (online) 2047-783X
    ISSN 0949-2321
    DOI 10.1186/s40001-024-01649-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4636: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: RNA Interference Ex Vivo.

    Liu, Shuang

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2766, Page(s) 145–151

    Abstract: RNA interference (RNAi) is a widely used technique to regulate the expression of genes and proteins with a high degree of specificity that is not easily accessed by traditional pharmacological approaches. For preclinical research on rheumatoid arthritis ( ...

    Abstract RNA interference (RNAi) is a widely used technique to regulate the expression of genes and proteins with a high degree of specificity that is not easily accessed by traditional pharmacological approaches. For preclinical research on rheumatoid arthritis (RA), silencing of target genes in primary immune cells can be easily achieved by the application of small interfering RNA (siRNA) and synthetic short hairpin RNA (shRNA). Cellular and systemic administration of siRNA or shRNA has been a significant advance in preclinical research on RA. In this chapter, the basic techniques for gene silencing in human-derived peripheral T cells using liposome-dependent siRNA transfection and lentiviral-mediated shRNA delivery, aiming at gene silencing of therapeutic targets, are introduced.
    MeSH term(s) Humans ; RNA Interference ; RNA, Small Interfering/genetics ; Gene Silencing ; Administration, Cutaneous ; Arthritis, Rheumatoid/genetics
    Chemical Substances RNA, Small Interfering
    Language English
    Publishing date 2024-01-25
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3682-4_15
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Lentiviral Production Platform.

    Liu, Shuang

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2766, Page(s) 163–168

    Abstract: Lentiviral-mediated transfection technique is a powerful tool for gene modification in preclinical studies. By using this technique, the desired gene modification can be achieved easily in immune cells, nondividing, and terminally differentiated cells, ... ...

    Abstract Lentiviral-mediated transfection technique is a powerful tool for gene modification in preclinical studies. By using this technique, the desired gene modification can be achieved easily in immune cells, nondividing, and terminally differentiated cells, including hematopoietic stem cells, neurons, and even tumor cells, which other viral vectors cannot do. The main considerations of therapeutic gene delivery using a lentiviral system are the risk of insertional mutagenesis and the immune reaction elicited by infected cells. Although some biosafety concerns need to be addressed before clinical trials in rheumatoid arthritis, the lentiviral system targeting therapeutic targets has been widely used for in vivo gene transfer in animal models. In this chapter, the protocols for production of viral particles and viral concentration are provided. As an alternative utilization, this lentiviral production platform could also be employed to produce a pseudotype severe acute respiratory syndrome-related coronavirus 2 in which the spike glycoprotein of SARS-CoV-2 was incorporated into pseudovirions for viral study.
    MeSH term(s) Animals ; Lentivirus/genetics ; Arthritis, Rheumatoid ; Cell Differentiation ; Gene Editing ; Genetic Therapy
    Language English
    Publishing date 2024-01-25
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3682-4_17
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Lentiviral-Mediated Systemic RNA Interference In Vivo.

    Liu, Shuang

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2766, Page(s) 153–161

    Abstract: The shRNA-encoding lentivirus has been widely used for gene manipulation in preclinical studies. It is a powerful tool for gene transfer and shows promise in its ability to efficiently transduce immune cells and hematopoietic stems cells, which are the ... ...

    Abstract The shRNA-encoding lentivirus has been widely used for gene manipulation in preclinical studies. It is a powerful tool for gene transfer and shows promise in its ability to efficiently transduce immune cells and hematopoietic stems cells, which are the initial therapeutic target of autoimmune diseases, and considering that gene manipulation of these cells is usually difficult to achieve using other techniques. In previous chapters, we have described how to produce concentrated shRNA-encoding lentiviral particles. Here, systemic in vivo application of lentivirus, including viral quantification prior to injection, intraperitoneal injection, and quantification of integrated provirus, is introduced.
    MeSH term(s) Humans ; RNA Interference ; Lentivirus/genetics ; RNA, Small Interfering/genetics ; Autoimmune Diseases ; Hematopoietic Stem Cells
    Chemical Substances RNA, Small Interfering
    Language English
    Publishing date 2024-01-25
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3682-4_16
    Database MEDical Literature Analysis and Retrieval System OnLINE

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