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Article: [Research progress of hepatic sinusoidal obstruction syndrome].

Ke, Z L / Liu, Y L

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology

2023 Volume 30, Issue 12, Page(s) 1298–1303

Abstract: Hepatic sinusoidal obstruction syndrome (HSOS) is a hepatic vascular disease that begins with injury to hepatic sinusoidal endothelial cells, and has a fatality rate of over 80% in its severe form. Therefore, early diagnosis and treatment are crucial to ... ...

Abstract	Hepatic sinusoidal obstruction syndrome (HSOS) is a hepatic vascular disease that begins with injury to hepatic sinusoidal endothelial cells, and has a fatality rate of over 80% in its severe form. Therefore, early diagnosis and treatment are crucial to delay HSOS progression and reduce mortality. However, clinicians' understanding of the disease is still insufficient, and the clinical manifestations of the disease are similar to liver diseases caused by other etiologies, resulting in a high rate of misdiagnosis. This article mainly introduces the HSOS recent developments in the etiology and pathogenesis, clinical manifestations and auxiliary examinations, diagnostic criteria, treatment, and prevention.
MeSH term(s)	Humans ; Hepatic Veno-Occlusive Disease/diagnosis ; Hepatic Veno-Occlusive Disease/etiology ; Endothelial Cells ; Pyrrolizidine Alkaloids ; Hepatic Veins
Chemical Substances	Pyrrolizidine Alkaloids
Language	Chinese
Publishing date	2023-02-27
Publishing country	China
Document type	English Abstract ; Journal Article
ISSN	1007-3418
ISSN	1007-3418
DOI	10.3760/cma.j.cn501113-20221110-00556
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: First Report of Pseudocercospora oenotherae Causing Leaf Spot in Hymenocallis littoralis in China

Zheng, C. / Liu, Y. L. / Xie, P.

Plant Disease. 2023 Sept. 01, v. 107, no. 9 p.2875-

2023

Abstract: Hymenocallis littoralis (Jacq.) Salisb. is a common ornamental plant in China. In November 2021, leaf spots were observed on H. littoralis in a public garden in Zhanjiang, Guangdong Province, China (21°17ʹ25″N, 110°18ʹ12″E). The disease incidence was 82% ...

Abstract	Hymenocallis littoralis (Jacq.) Salisb. is a common ornamental plant in China. In November 2021, leaf spots were observed on H. littoralis in a public garden in Zhanjiang, Guangdong Province, China (21°17ʹ25″N, 110°18ʹ12″E). The disease incidence was 82% (n = 100 investigated plants from about 10 ha). Initial small white dots were densely distributed on the leaves, and they gradually expanded into round lesions with purple centers typically surrounded by yellow halos. The coalescence of the individual spot eventually led to leaf wilt. Ten symptomatic leaves from 10 plants were sampled. The margins of the samples were cut into 2 × 2 mm pieces. The tissue surface was disinfected with 75% ethanol for 30 s and 2% sodium hypochlorite for 60 s. Thereafter, the samples were rinsed three times in sterile water, placed on potato dextrose agar (PDA), and incubated at 28°C. Pure cultures were obtained by transferring hyphal tips to new PDA plates. Twenty-eight isolates were obtained (isolation frequency = 28/4 × 10 = 70%). Three representative single-spore isolates (HPO-1, HPO-2, and HPO-3) by the single-spore isolation method (Fang 1998) were used for further study. The colonies of the isolates on PDA were olive green in 7 days at 28°C. Conidiogenous cells were unbranched, straight to geniculate–sinuous, tapered toward the apex, and 12 to 19 × 3 μm (n = 20). Conidia were solitary, smooth, straight or curved, pale brown, 3 to 8 septate, apex acute, base truncate, and 55.3 to 86.5 × 2.0 to 3.5 μm (n = 50). The morphological characteristics were consistent with the description of Pseudocercospora oenotherae (Guo and Liu 1992; Kirschner 2015). For molecular identification, the colony PCR method with Taq DNA polymerase and MightyAmp DNA polymerase (Lu et al. 2012) was used to amplify the internal transcribed spacer (ITS), translation elongation factor 1-α gene (TEF1), and actin (ACT) loci of the isolates using the primer pairs ITS1/ITS4, EF1/EF2, and ACT-512F/ACT-783R, respectively (O’Donnell et al. 1998). Their sequences were deposited in GenBank under accession numbers OM654573 to OM654575 (ITS), OM831379 to OM831381 (TEF1), and OM831349 to OM831351 (ACT). A phylogenetic tree was generated on the basis of the concatenated data from the sequences of ITS, TEF1, and ACT that clustered the isolates with P. oenotherae (the type strain CBS 131920). Pathogenicity testing was performed in a greenhouse with 80% relative humidity at 28 to 30°C. Healthy plants of H. littoralis were grown in pots, with one plant in each pot. They were inoculated with a spore suspension (1 × 10⁵ spores/ml) of the isolates and sterile distilled water (control). Sterile cotton balls were immersed in the spore suspension and sterile distilled water for about 15 s before they were adhered to the leaves for 3 days. Each isolate was inoculated with three plants (1 month old), and each plant was inoculated with two leaves. The test was performed three times. Symptoms were found on the inoculated plants after 2 weeks with the disease incidence of 88.89%, whereas the control plants remained healthy. The fungus was reisolated from the infected leaves and confirmed as the same isolates by morphological and ITS analyses. No fungus was isolated from the control plants. P. oenotherae has caused leaf spot on Oenothera biennis L. (Guo and Liu 1992). To the best of our knowledge, this is the first report that H. littoralis is the second host of P. oenotherae (Crous et al. 2013). Thus, this work provides an important reference for the control of this disease in the future.
Keywords	DNA-directed DNA polymerase ; Hymenocallis littoralis ; Oenothera biennis ; Pseudocercospora ; actin ; conidia ; cotton ; culture media ; disease incidence ; ethanol ; fungi ; genes ; greenhouses ; hyphae ; internal transcribed spacers ; isolation techniques ; leaf spot ; leaves ; ornamental plants ; pathogenicity ; peptide elongation factors ; phylogeny ; public gardens ; relative humidity ; sodium hypochlorite ; China ; Pseudocercospora oenotherae
Language	English
Dates of publication	2023-0901
Publishing place	The American Phytopathological Society
Document type	Article ; Online
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-08-22-1897-PDN
Database	NAL-Catalogue (AGRICOLA)

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Article: First Report of Phoma herbarum Causing Leaf Spot on Rhapis humilis in China

Xie, P. / Zhong, F. T. / Liu, Y. L.

Plant disease. 2022 Feb. 28, v. 106, no. 2

2022

Abstract: Rhapis humilis Blume is an ornamental plant for landscaping that is widely distributed in China. In February 2020, a leaf spot disease was observed on R. humilis in a nursery shed in Zhanjiang (21.17°N, 110.18°E), Guangdong, China. The disease incidence ... ...

Abstract	Rhapis humilis Blume is an ornamental plant for landscaping that is widely distributed in China. In February 2020, a leaf spot disease was observed on R. humilis in a nursery shed in Zhanjiang (21.17°N, 110.18°E), Guangdong, China. The disease incidence was more than 90%. The early symptom was small water-soaked lesions, which then turned into black necrotic spots. Eventually, the individual lesions coalesced into larger ones, leading to the death of diseased leaves. Ten diseased leaves were collected from the nursery. The diseased tissues were cut into 2 × 2-mm pieces, surface disinfected with 75% ethanol for 30 s and 2% sodium hypochlorite for 60 s, and then rinsed three times with sterile water before pathogen isolation. The tissues were plated on potato dextrose agar (PDA) medium and incubated at 28°C in the dark for 4 days. Pure cultures were produced by transferring hyphal tips to new PDA plates. Three isolates (RHPH-1, RHPH-2, and RHPH-3) were obtained. The colonies of the isolates were approximately 5 cm in diameter after 7 days. They were initially whitish and later became grayish white. The NaOH testing on MEA cultures was negative. No sporulation was detected after 30 days. The fertile structures of the specimens collected in the nursery were examined. Pycnidia were globose, measured 68 to 265 × 72 to 360 µm (n = 20), and mostly embedded. Conidia were aseptate, hyaline, and ellipsoid, measuring 3.6 to 6.5 × 2.2 to 2.7 µm (n = 30). Based on the morphological characteristics, the fungus was identified as in genus Phoma (Boerema et al. 2004). For molecular identification, the colony PCR method with MightyAmp DNA Polymerase (Takara-Bio, Dalian, China) (Lu et al. 2012) was used to amplify the internal transcribed spacer (ITS), partial RNA polymerase II largest subunit (RPB2), and beta-tubulin (β-tub) loci of three isolates using primer pairs ITS4/ITS5, RPB2-6F/RPB2-7R, and BT2a/BT2b, respectively (Chen et al. 2015; White et al. 1990). The sequences were deposited in GenBank (ITS, MZ419364 to MZ419366; RPB2, MZ562293 to MZ562295; and β-tub, MZ562296 to MZ562298). Based on BLAST analysis, the sequences of the ITS, RPB2, and β-tub all showed 100% similarity to Phoma herbarum Westend. (CBS 377.92; accession nos. KT389536 for ITS, KT389663 for RPB2, and KT389837 for β-tub). Pathogenicity testing was performed in a greenhouse with 80% relative humidity at 25 to 30°C. Ten healthy plants of R. humilis were grown in pots, with one plant in each pot. The leaves were pinpricked with sterile needles before inoculation. They were inoculated with mycelial plugs of the isolates or sterile agar plugs (as control), with four plugs for each leaf. Five plants were used in each treatment. Disease symptoms similar to those in the nursery were observed on the inoculated plants 2 weeks after inoculation, whereas the control plants remained healthy. The fungus was reisolated from the symptomatic leaves and confirmed as P. herbarum by morphology and ITS analysis. P. herbarum was reported to cause leaf spot on Atractylodes lancea, Camellia sinensis, Elaeis guineensis, Lilium brownii, and Vetiveria zizanioides in China; Bituminaria bituminosa, Glycine max, Medicago sativa, and Pisum sativum in Australia; and Salvia nemorosa in Italy (Li et al. 2011, 2012; Thangaraj et al. 2018). To the authors’ knowledge, the present study was the first to report P. herbarum causing leaf spot on R. humilis in China. P. herbarum seriously affected the supply of seedlings in R. humilis, and its epidemiology on R. humilis should be further studied.
Keywords	Atractylodes lancea ; Bituminaria bituminosa ; Camellia sinensis ; Chrysopogon zizanioides ; DNA-directed DNA polymerase ; DNA-directed RNA polymerase ; Elaeis guineensis ; Glycine max ; Lilium brownii ; Medicago sativa ; Phoma ; Pisum sativum ; Salvia nemorosa ; agar ; conidia ; culture media ; death ; disease incidence ; ethanol ; fungi ; greenhouses ; hyphae ; internal transcribed spacers ; leaf spot ; leaves ; mycelium ; ornamental plants ; pathogenicity ; pathogens ; pycnidia ; relative humidity ; sodium hypochlorite ; sporulation ; tubulin ; Australia ; China ; Italy
Language	English
Dates of publication	2022-0228
Publishing place	The American Phytopathological Society
Document type	Article
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-07-21-1468-PDN
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: First Report of Pseudocercospora paraguayensis Causing Leaf Spots on Bixa orellana from the Chinese Mainland

Liu, C. Y. / Yang, S. X. / Liu, Y. L.

Plant Disease. 2023 Oct. 02, v. 107, no. 10 p.3309-

2023

Abstract: Bixa orellana L. is a traditional Chinese medicine. In December 2019, a leaf spot disease was observed on B. orellana from a field in Zhanjiang (21°18′12″N, 110°17′22″E), China. Disease incidence was around 85% (n = 100 investigated plants from about 30 ... ...

Abstract	Bixa orellana L. is a traditional Chinese medicine. In December 2019, a leaf spot disease was observed on B. orellana from a field in Zhanjiang (21°18′12″N, 110°17′22″E), China. Disease incidence was around 85% (n = 100 investigated plants from about 30 ha). Initial leaf spots were circular, and the center of the lesions was grayish-white with a purple-black border. The coalescence of individual spots eventually led to leaf wilt. Ten symptomatic leaves from 10 plants were sampled. The margins of the samples were cut into 2 × 2 mm pieces, and the surfaces were disinfected with 75% ethanol for 30 s and 2% sodium hypochlorite for 60 s. The samples were then rinsed three times in sterile water, plated on potato dextrose agar (PDA), and incubated at 28°C. Pure cultures were obtained by transferring the hyphal tips to new PDA plates. Three representative isolates (BOPP-1, BOPP-2, and BOPP-3) were used for further study. The colonies of isolates on PDA were dark olive green with off-white aerial mycelia after 7 days at 28°C. Conidia were solitary, smooth to verrucous, olive to light brown, slightly curved, and narrowly obclavate, with an obtuse apex and obconic to truncate base, had 2 to 4 septa, and were 30.4 to 55.5 × 2.0 to 3.5 μm in size. These morphological characteristics did not differ from the description of Pseudocercospora paraguayensis (Crous et al. 1997). For molecular identification, the internal transcribed spacer (ITS) region, translation elongation factor 1-α (TEF1) gene, and actin (ACT) gene were amplified using the primer pairs ITS1/ITS4 (White et al. 1990), EF1/EF2 (O’Donnell et al. 1998), and ACT-512F/ACT-783R (Carbone and Kohn 1999) and sequenced from DNA extracted from the three isolates, respectively. Sequences were deposited in GenBank under accession numbers MZ363823 to MZ363825 (ITS), MZ614954 to MZ614956 (TEF1), and MZ614951 to MZ614953 (ACT). A phylogenetic tree was generated on the basis of the concatenated data from the sequences of ITS, TEF1, and ACT, showing that the three isolates nested within the clade containing the type specimen of P. paraguayensis (CBS 111286) but not within P. bixae (the type specimen CPC 25244). Pathogenicity was tested through in vivo experiments. Inoculation and control seedlings (n = 5, 1 month old) were sprayed with a spore suspension (1 × 10⁵ spores/ml) of P. paraguayensis and sterile distilled water (control), respectively, until runoff (Fang 1998). The plants were grown in pots in a greenhouse at 28°C, with approximately 80% relative humidity. The test was performed three times. Symptoms similar to those in the field were observed on the inoculated plants after 2 weeks. The control plants remained healthy. The fungus was reisolated from the infected leaves and confirmed as the same isolates by morphological analysis and comparison of ITS sequences with 100% identity to those of the isolates. No original fungi were isolated from the control plants. A previous study reported that P. paraguayensis caused leaf spots on pistachio and eucalyptus, and the fungus causing the leaf spots of B. orellana was redescribed as P. bixae (Crous et al. 2019). However, multilocus phylogenetic analyses differentiated P. paraguayensis from P. bixae. In the present study, P. paraguayensis was distinguished from P. bixae because of the absence of catenulate conidia and the presence of finely verruculose conidia (Crous et al. 2013). P. eucalypti as a synonym was reported in Taiwan (https://www.MycoBank.org). The current study is the first to report P. paraguayensis causing leaf spots on B. orellana from the Chinese mainland. This finding will help provide a scientific basis for disease detection.
Keywords	Bixa orellana ; DNA ; Eucalyptus ; Oriental traditional medicine ; Pseudocercospora ; actin ; conidia ; culture media ; disease detection ; disease incidence ; ethanol ; fungi ; genes ; greenhouses ; hyphae ; internal transcribed spacers ; leaf spot ; leaves ; mycelium ; olives ; pathogenicity ; peptide elongation factors ; phylogeny ; pistachios ; relative humidity ; runoff ; sodium hypochlorite ; China ; Taiwan ; leaf spots ; Pseudocercospora paraguayensis
Language	English
Dates of publication	2023-1002
Publishing place	The American Phytopathological Society
Document type	Article ; Online
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-05-23-1023-PDN
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: First Report of Rhizoctonia solani AG1-IA Causing Rice Sheath Blight of Oryza rufipogon in China

Huang, Y. X. / Yang, S. X. / Liu, Y. L.

Plant Disease. 2023 Oct. 02, v. 107, no. 10 p.3295-

2023

Abstract: Red rice (Oryza rufipogon Griff.) is a valuable source of important agronomic traits as well as genes for biotic and abiotic stress tolerance. In June 2020, rice sheath blight on O. rufipogon cultivar Bin09 was observed in Zhanjiang (20.93°N, 109.79°E), ... ...

Abstract	Red rice (Oryza rufipogon Griff.) is a valuable source of important agronomic traits as well as genes for biotic and abiotic stress tolerance. In June 2020, rice sheath blight on O. rufipogon cultivar Bin09 was observed in Zhanjiang (20.93°N, 109.79°E), China. Initial symptoms on sheaths were water-soaked and light green lesions. Then, the lesions gradually expanded into oval- or cloud-shaped lesions with a gray-white center. The lesions coalesced, causing the entire sheath to become blighted. Disease incidence reached approximately 30% in the fields (10 ha) surveyed. Twenty sheaths with symptoms were collected and cut into pieces of 2 × 2 cm in size. They were surface disinfected with 75% ethanol for 30 s and 2% sodium hypochlorite (NaOCl) for 60 s, rinsed three times with sterile water, blotted dry on sterile paper, plated on potato dextrose agar (PDA), and incubated at 28°C in the dark for 4 days. Thirty-six pure cultures were obtained by transferring hyphal tips to new PDA plates, and three isolates (ORRS-1, ORRS-2, and ORRS-3) with similar morphological characteristics on PDA were selected as the representative isolates for study. The colony of the isolate ORRS-1 was initially white and then turned brown with brown sclerotia. Septate hyphae were hyaline, smooth, and branched at right angles with a septum near the point of branching. Based on these morphological characteristics, the fungus was identified as Rhizoctonia solani Kuhn (Sneh et al. 1991). The isolates were deposited in the fungus collection of the Aquatic Organisms Museum of Guangdong Ocean University. For molecular identification, genomic DNA from each of the three isolates was extracted, and the internal transcribed spacer (ITS) region was amplified and sequenced with the primer pair ITS5/ITS4 (White et al. 1990). The sequences were deposited in GenBank (accession nos. OP497977 to OP497979). The three isolates were 100% identical (716/716, 716/716, and 716/716 bp) with those of R. solani AG1-IA (accession nos. KX674518, MK481078, and MK480532) in BLAST analysis. The phylogenetic tree grouped the three isolates within the R. solani AG1-IA clade with high bootstrap support (99%) by the maximum likelihood method. A pathogenicity test was performed with these three isolates in a greenhouse at 24 to 30°C. Approximately 50 seedlings of red rice cultivar Bin09 were grown in each cup (250 ml in size with 50 cm³ of sterile soil). At the three-leaf stage, plants in five cups were inoculated with each isolate by spraying a mycelial suspension (10⁶ mycelial fragments/ml) until runoff. The mycelial suspension was prepared by adding sterile distilled water to the cultures and gently scraping the surface with a sterilized scalpel blade. Five plants sprayed with sterile water served as the controls. The test was conducted three times. Sheath blight was observed on the inoculated leaves after 15 days, whereas no disease was observed in the control plants. Morphological characteristics and the ITS sequences of fungal isolates reisolated from the diseased sheaths were identical to those of R. solani AG1-IA. R. solani AG1-IA is one of the most important plant pathogens worldwide, causing foliar diseases on maize, rice (O. sativa L.), and soybean (Bernardes-de-Assis et al. 2009). To our knowledge, this is the first report of R. solani AG1-IA causing rice sheath blight of O. rufipogon in China (Farr and Rossman 2022). With the spread of the pathogen on weedy populations of red rice, resistant races or pathotypes may evolve that could spread to cultivated rice.
Keywords	DNA ; Oryza rufipogon ; Thanatephorus cucumeris ; abiotic stress ; corn ; cultivars ; culture media ; disease incidence ; ethanol ; fungi ; greenhouses ; hyphae ; internal transcribed spacers ; leaf blight ; museums ; mycelium ; oligodeoxyribonucleotides ; paper ; pathogenicity ; pathogens ; pathotypes ; phylogeny ; red rice ; runoff ; sclerotia ; sodium hypochlorite ; soil ; soybeans ; statistical analysis ; stress tolerance ; China ; Rhizoctonia solani AG1-IA ; rice sheath blight
Language	English
Dates of publication	2023-1002
Publishing place	The American Phytopathological Society
Document type	Article ; Online
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-04-23-0716-PDN
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: First Report of Mucor irregularis Causing Flower Rot on Selenicereus undatus in China

Li, H. Y. / Liu, C. Y. / Liu, Y. L.

Plant Disease. 2023 Aug. 01, v. 107, no. 8 p.2520-

2023

Abstract: Dragon fruit (Selenicereus undatus [Haw.] D.R.Hunt) is a famous tropical fruit (Korotkova et al. 2017). In May 2021, a flower rot disease was found on dragon fruit in a field (21°19′42″N, 110°28′32″E) located in Zhanjiang, Guangdong Province, China. The ... ...

Abstract	Dragon fruit (Selenicereus undatus [Haw.] D.R.Hunt) is a famous tropical fruit (Korotkova et al. 2017). In May 2021, a flower rot disease was found on dragon fruit in a field (21°19′42″N, 110°28′32″E) located in Zhanjiang, Guangdong Province, China. The disease incidence rate was approximately 30% (n = 500 investigated plants from about 30 ha). Flower rot was evident, and the affected area was light brown, watery, and soft and covered with white mycelia. The pathogen continued to infect the fruit during the fruit ripening stage with about 20% rot rate. Ten samples of symptomatic flowers were collected from the field. Margins of the diseased tissue were cut into 2 × 2-mm pieces. The pieces were surface disinfected with 75% ethanol for 30 s and 2% sodium hypochlorite for 60 s. Pure cultures were obtained by transferring hyphal tips onto new PDA plates. Three representative isolates (HUM-1, HUM-2, and HUM-3) produced by single-spore isolation were randomly selected for further study. Colonies on PDA were circular with massive aerial hyphae, white to ochraceous in color. Nonseptate hyphae were hyaline. Sporangiophores arose from hyphae. Sporangiospores were hyaline, smooth-walled, mostly subspherical to ellipsoidal, and measured 3.15 to 6.55 × 1.35 to 2.85 μm (n = 50). Morphological characteristics of the isolates were consistent with the description of Mucor irregularis (Lima et al. 2018). Molecular identification was done using the colony PCR method with MightyAmp DNA Polymerase (Takara Bio, Dalian, China; Lu et al. 2012), which was used to amplify the internal transcribed spacer (ITS) region and large subunit (LSU) with ITS1/ITS4 and LR0R1/LR5, respectively (Vilgalys et al. 1990). The amplicons were sequenced, and the sequences were deposited in GenBank with accession numbers OL376751 to OL376753 for ITS and OM672239 to OM672241 for LSU. BLAST analysis of these sequences revealed 100% identity with M. irregularis in GenBank. The sequences were also concatenated for phylogenetic analysis by the maximum likelihood method. The isolates clustered with M. irregularis (the type strain CBS 103.93). Pathogenicity was tested through in vivo experiments. Nine healthy flowers of dragon fruit were inoculated with 3-day-old mycelial plugs (5 × 5 mm) of the isolates, while another five healthy flowers were treated with PDA plugs (controls). The plugs were embedded inside the calyxes, and each flower was inoculated with one plug per calyx. Besides, the inoculated and control flowers (n = 5) were sprayed with a spore suspension (1 × 10⁵ spores/ml) of the three isolates individually and sterile distilled water, respectively, until runoff (Feng and Li 2019). The plants were grown in pots in a greenhouse at 28°C with approximately 80% relative humidity. The test was repeated three times. After 3 days of incubation, rot symptoms appeared on the inoculated flowers, which were similar to those observed on the naturally infected samples in the field. The control flowers remained healthy. The fungus was reisolated from the inoculated flowers and confirmed as M. irregularis by morphology and ITS analysis. M. irregularis was reported as a pathogen causing human skin diseases and postharvest diseases of crops (Álvarez et al. 2011; Lima et al. 2018; Wang et al. 2022). This is the first report of M. irregularis causing flower rot of dragon fruit and reducing fruit yield in China. This research can provide a theoretical basis for the fruit industry to maintain yield.
Keywords	DNA-directed DNA polymerase ; Hylocereus ; Mucor ; Selenicereus ; calyx ; color ; disease incidence ; ethanol ; fruit yield ; fruits ; fungi ; greenhouses ; hyphae ; industry ; internal transcribed spacers ; mycelium ; pathogenicity ; pathogens ; phylogeny ; relative humidity ; runoff ; skin (animal) ; sodium hypochlorite ; spores ; statistical analysis ; China ; flower rot ; Mucor irregularis ; Selenicereus undatus
Language	English
Dates of publication	2023-0801
Publishing place	The American Phytopathological Society
Document type	Article ; Online
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-01-23-0117-PDN
Database	NAL-Catalogue (AGRICOLA)

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Article: First Report of

Huang, R H / Zhong, F T / Liu, Y L / Chen, J G

Plant disease

2022 , Page(s) PDIS08211859PDN

Language	English
Publishing date	2022-04-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	754182-x
ISSN	0191-2917
ISSN	0191-2917
DOI	10.1094/PDIS-08-21-1859-PDN
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: [Progress in diagnosis and treatment of obstructive sleep apnea in pregnancy].

Liu, Y L / Wang, B / Huang, R

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases

2021 Volume 44, Issue 2, Page(s) 124–127

MeSH term(s)	Female ; Humans ; Obesity/complications ; Pregnancy ; Pregnancy Complications/diagnosis ; Pregnancy Complications/therapy ; Sleep Apnea, Obstructive/diagnosis ; Sleep Apnea, Obstructive/etiology ; Sleep Apnea, Obstructive/therapy
Language	Chinese
Publishing date	2021-01-30
Publishing country	China
Document type	Journal Article ; Review
ZDB-ID	1027965-9
ISSN	1001-0939
ISSN	1001-0939
DOI	10.3760/cma.j.cn112147-20200525-00634
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Article ; Online: Efficacy and safety of dupilumab in the treatment of kimura's disease.

Liu, Y L / Ran, Y T / Zhang, Y F / Peng, X T / Xia, Y M / Yan, H L

QJM : monthly journal of the Association of Physicians

2024

Abstract: Background: Kimura's disease (KD) is a rare chronic inflammatory condition characterized by nodules and lymphadenopathy in the head and neck region, exhibiting type II inflammation. Dupilumab is commonly used against type II inflammation.: Aim: To ... ...

Abstract	Background: Kimura's disease (KD) is a rare chronic inflammatory condition characterized by nodules and lymphadenopathy in the head and neck region, exhibiting type II inflammation. Dupilumab is commonly used against type II inflammation. Aim: To evaluate the efficacy and safety of dupilumab in KD patients. Design: The real-world study was conducted in a hospital in China. Methods: Six male patients with a mean age of 24.50 ± 15.47 years were treated with dupilumab following the same protocol as that for atopic dermatitis (AD). Clinical and laboratory indicators, such as maximum nodule diameter, blood eosinophil count, eosinophil percentage, and total serum IgE levels were assessed at baseline, week 12, and week 24. Adverse events were documented. Paired t-tests and one-way ANOVA were used for statistical analysis. Results: The results showed significant reductions in the longest nodule diameter at week 12 (P = 0.008) and week 24 (P = 0.001) compared to baseline. Blood eosinophil count decreased by 57.95% (P = 0.024) at week 12 and 90.59% (P = 0.030) at week 24. Eosinophil percentage decreased by 58.44% (P = 0.026) at week 12 and 89.37% (P = 0.013) at week 24. Total serum IgE levels decreased by 78.02% (P = 0.040) at week 12 and 89.55% (P = 0.031) at week 24. The presence of AD did not affect the results. One patient experienced temporary facial erythema after 32 weeks of treatment, which resolved with topical treatment. No other adverse events were reported. Conclusion: Dupilumab demonstrated effectiveness in treating KD without severe adverse events.
Language	English
Publishing date	2024-03-16
Publishing country	England
Document type	Journal Article
ZDB-ID	1199985-8
ISSN	1460-2393 ; 0033-5622 ; 1460-2725
ISSN (online)	1460-2393
ISSN	0033-5622 ; 1460-2725
DOI	10.1093/qjmed/hcae048
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Article: [Combinational Overexpression of Foxa3 and Hnf4α Enhance the Proliferation and Prolong the Functional Maintenance of Primary Hepatocytes].

Fan, J Y / Dama, G / Liu, Y L / Guo, W Y / Lin, J T

Molekuliarnaia biologiia

2023 Volume 57, Issue 4, Page(s) 668–670

Abstract: In an in vitro culture system, primary hepatocytes usually display a low proliferation capacity, accompanied with a decrease of viability and a loss of hepatocyte-specific functions. Previous studies have demonstrated that the combination introductions ... ...

Abstract	In an in vitro culture system, primary hepatocytes usually display a low proliferation capacity, accompanied with a decrease of viability and a loss of hepatocyte-specific functions. Previous studies have demonstrated that the combination introductions of certain hepatocyte-specific transcription factors are able to convert fibroblasts into functional hepatocyte-like cells. However, such combinational usage of transcription factors in primary hepatocytes culture has not yet sufficiently studied. The forkhead box protein A3 (FoxA3) and hepatocyte nuclear factor 4α (Hnf4α) are liver-enriched transcription factors that play vital roles in the differentiation, and maintenance of hepatocytes. Thus, we simultaneously overexpressed the two genes, Foxa3 and Hnf4α, in rat hepatocytes and observed that the combinational augmentation of these two transcription factors have enhanced the proliferation and stabilized the hepatocyte-specific functions of primary hepatocytes over a long-term culture period.
MeSH term(s)	Animals ; Rats ; Cell Differentiation/genetics ; Cell Proliferation/genetics ; Hepatocytes/metabolism ; Liver/metabolism ; Penicillins/metabolism ; Transcription Factors/genetics
Chemical Substances	Penicillins ; Transcription Factors ; Hnf4a protein, rat ; Foxa3 protein, rat
Language	Russian
Publishing date	2023-08-02
Publishing country	Russia (Federation)
Document type	English Abstract ; Journal Article
ZDB-ID	213542-5
ISSN	0026-8984
ISSN	0026-8984
DOI	10.31857/S0026898423040031, EDN: QKQQUW
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