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Article ; Online: Mesenchymal Stem Cell-Laden In Situ-Forming Hydrogel for Preventing Corneal Stromal Opacity.

Liu, Yinan / Hong, Jing

2024 Volume 43, Issue 5, Page(s) 609–626

Abstract: Purpose: The aims of this study were to construct a mesenchymal stem cell (MSC)-laden in situ-forming hydrogel and study its effects on preventing corneal stromal opacity.: Methods: The native gellan gum was modified by high temperature and pressure, ...

Abstract	Purpose: The aims of this study were to construct a mesenchymal stem cell (MSC)-laden in situ-forming hydrogel and study its effects on preventing corneal stromal opacity. Methods: The native gellan gum was modified by high temperature and pressure, and the rabbit bone marrow MSCs were encapsulated before adding Ca 2+ to initiate cross-linking. The effects of the hydrogel on 3D culture and gene expression of the rabbit bone marrow MSCs were observed in vitro. Then, the MSC-hydrogel was used to repair corneal stromal injury in New Zealand white rabbits within 28 days postoperation. Results: The short-chain gellan gum solution has a very low viscosity (<0.1 Pa·s) that is ideal for encapsulating cells. Moreover, mRNA expressions of 3D-cultured MSCs coding for corneal stromal components (decorin, lumican, and keratocan) were upregulated (by 127.8, 165.5, and 25.4 times, respectively) ( P < 0.05) on day 21 in vitro and were verified by Western blotting results. For the in vivo study, the corneal densitometry of the experimental group was (20.73 ± 1.85) grayscale units which was lower than the other groups ( P < 0.05). The MSC-hydrogel downregulated mRNA expression coding for fibrosis markers (α-smooth muscle actin, vimentin, collagen type 5-α1, and collagen type 1-α1) in the rabbit corneal stroma. Furthermore, some of the 5-ethynyl-2'-deoxyuridine (EdU)-labeled MSCs integrated into the upper corneal stroma and expressed keratocyte-specific antigens on day 28 postoperation. Conclusions: The short-chain gellan gum allows MSCs to slowly release to the corneal stromal defect and prevent corneal stromal opacity. Some of the implanted MSCs can integrate into the corneal stroma and differentiate into keratocytes.
MeSH term(s)	Animals ; Rabbits ; Hydrogels ; Cornea/metabolism ; Corneal Stroma/metabolism ; Corneal Keratocytes ; Mesenchymal Stem Cells ; Corneal Opacity/prevention & control ; Corneal Opacity/metabolism ; Corneal Injuries/metabolism ; Collagen/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
Chemical Substances	Hydrogels ; Collagen (9007-34-5) ; RNA, Messenger
Language	English
Publishing date	2024-01-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	604826-2
ISSN	1536-4798 ; 0277-3740
ISSN (online)	1536-4798
ISSN	0277-3740
DOI	10.1097/ICO.0000000000003475
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Article ; Online: A Method for Prediction of High-Water-Production Areas in Coalbed Methane Blocks Based on γ Logging Data: A Case Study of the Taiyuan Formation in Liulin Area, Eastern Ordos Basin, China.

Liu, Yinan / Wang, Li / Xu, Yanyong / Zong, Peng / Dong, Yu

ACS omega

2024 Volume 9, Issue 8, Page(s) 9577–9584

Abstract: The roof aquifer of the Carboniferous Taiyuan Formation coal beds in the Liulin area severely restricts the development and utilization of coalbed methane (CBM). A method for quantitatively predicting high-water-production areas was established by ... ...

Abstract	The roof aquifer of the Carboniferous Taiyuan Formation coal beds in the Liulin area severely restricts the development and utilization of coalbed methane (CBM). A method for quantitatively predicting high-water-production areas was established by analyzing the relationship between the geophysical logging data and water production. The results showed that the logging profile of the limestone aquifers in high-water-production wells was unique, with high acoustic velocity (AC), high γ-ray values (GR), and low resistivity (Rd). The developed pores and fractures in the roof limestone increase the interval transit time. The formation water in the pores and fractures of the roof limestone decreases the resistivity. The clay filling in the pores and fractures of the roof limestone originated from the dissolution product of limestone and hydrodynamic transportation, which resulted in increased GR values. Furthermore, the representative natural GR log data were used to calculate the clay content in limestone, which indicated that the clay content in limestone had a positive correlation with the water yield of the CBM wells. The water-bearing characteristics of roof limestone showed that the water content was higher in the northern area and decreased gradually toward the south. The method for predicting the high-water-production area was helpful for the CBM exploration and production.
Language	English
Publishing date	2024-02-14
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.3c09453
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Article: Comparisons of the clinical outcomes of Centurion

Liu, Yinan / Hong, Jing / Chen, Xiaoyong

Frontiers in medicine

2023 Volume 10, Page(s) 1294808

Abstract: Background: During cataract phacoemulsification surgery, the Alcon Centurion with Active Sentry can achieve a more stable anterior chamber, which allows a lower intraocular pressure (IOP) setting than the gravity fluidics system. In this randomized ... ...

Abstract	Background: During cataract phacoemulsification surgery, the Alcon Centurion with Active Sentry can achieve a more stable anterior chamber, which allows a lower intraocular pressure (IOP) setting than the gravity fluidics system. In this randomized controlled trial, we compared these two systems' damage to the cornea under different IOP settings. Methods: Seventy-eight eyes of 53 patients with corneal endothelial cell density (ECD) of 500∼1500/mm Results: We observed no significant differences in best corrected visual acuity (BCVA), cumulative dissipated energy (CDE), total case time, estimated fluidics usage (EFU) and ophthalmic viscoelastic devices (OVDs) usage between the two groups. The enrolled eyes were further divided into soft nucleus (27 eyes) and hard nucleus (51 eyes) subgroups. And we found less pain complaint during surgeries, lower corneal edema ratio at 1-day and 1-week visit, smaller CCT changes at 1-day visit and lower ECD loss rate at 1-month visit ( Conclusion: Owing to a lower IOP setting, Centurion Clinical trial registration: https://www.chictr.org.cn, identifier ChiCTR2300077865.
Language	English
Publishing date	2023-11-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2775999-4
ISSN	2296-858X
ISSN	2296-858X
DOI	10.3389/fmed.2023.1294808
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Article ; Online: Three-dimensional changes in the upper airway and craniomaxillofacial morphology of patients with Angle Class III malocclusion treated with a Frankel III appliance.

Liu, Yinan / Yang, Kai

BMC oral health

2021 Volume 21, Issue 1, Page(s) 634

Abstract: Background: Angle Class III malocclusion, characterized by a concave profile, can cause serious harm to children's physical and mental health. The Frankel III appliance is an effective treatment for Angle Class III malocclusion in mixed denition. We ... ...

Abstract	Background: Angle Class III malocclusion, characterized by a concave profile, can cause serious harm to children's physical and mental health. The Frankel III appliance is an effective treatment for Angle Class III malocclusion in mixed denition. We explored three-dimensional changes in the upper airway and craniomaxillofacial morphology, after one year of Frankel III appliance treatment, in children with Angle Class III malocclusion. Methods: We included 20 children (9 males), aged 8-10 years, with Angle Class III malocclusion from the Orthodontics Department of our hospital. Each child was treated with a Frankel III appliance for one year. Cone beam computed tomography was performed before and after treatment to evaluate three-dimensional changes in the upper airway and craniomaxillofacial morphology. Results: After one year of treatment, in the upper airway, we observed significant increases in the nasopharynx volume and height (P < 0.05); the velopharyngeal volume, height, and average cross-sectional area (P < 0.05); the glossopharynx volume and minimum cross-sectional area (P < 0.05); and the laryngopharynx height (P < 0.05). Accordingly, the total upper airway volume, height, and average cross sectional area increased significantly (P < 0.05). An examination of craniomaxillofacial morphology showed significant increases in some bone tissues (P < 0.05) and dental measurements, and a significant reduction in the inclination of the mandibular central incisor (P < 0.05). Conclusion: Children with Angle class III malocclusion treated with a Frankel III appliance showed no upper airway narrowing, even after repositioning the mandible posteriorly. Moreover, treatment promoted forward maxilla development and increased its width, in both the dental arch and alveolar bone, which provided a more harmonious craniofacial morphology.
MeSH term(s)	Cephalometry ; Child ; Cone-Beam Computed Tomography ; Humans ; Male ; Malocclusion, Angle Class II ; Malocclusion, Angle Class III/diagnostic imaging ; Malocclusion, Angle Class III/therapy ; Mandible ; Maxilla/diagnostic imaging
Language	English
Publishing date	2021-12-09
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2091511-1
ISSN	1472-6831 ; 1472-6831
ISSN (online)	1472-6831
ISSN	1472-6831
DOI	10.1186/s12903-021-02013-0
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Article ; Online: Quantitative Characterization of Inorganic Pores in Sinian Doushantou Dolomitic Shale Based on FIB-SEM in Western Hubei Province, China.

Liu, Yinan / Zong, Peng / Wang, Li / Xu, Yanyong / Guo, Jingzhen / Wu, Heng

ACS omega

2024 Volume 9, Issue 7, Page(s) 8151–8161

Abstract: Unlike traditional shale gas reservoirs, where organic matter pores dominate, inorganic pores are the primary reservoir space in the Sinian (Ediacaran) high-maturity Doushantou dolomitic shale in western Hubei Province, China. The inorganic pore ... ...

Abstract	Unlike traditional shale gas reservoirs, where organic matter pores dominate, inorganic pores are the primary reservoir space in the Sinian (Ediacaran) high-maturity Doushantou dolomitic shale in western Hubei Province, China. The inorganic pore characteristics of Doushantuo shale and its influence on shale gas aggregation were investigated by examining the TOC content, thermal maturity, mineralogical composition, and field-emission scanning electron microscopy (SEM) and focused ion beam scanning electron microscopy (FIB-SEM) of drill cores. The results show that the shale mineral composition in the study area is primarily dolomite and plate-shaped interparticle-intercrystalline pores associated with dolomite are widespread inorganic pores in dolomitic shale. Interparticle-intercrystalline pores account for 75% of the total pores, with a pore size distribution mainly between 50 and 300 nm, as extracted from the 3D pore network model (PNM). Compared with organic pores, interparticle-intercrystalline pores provide greater space for gas storage and have a strong coupling relationship with the hydrocarbon generation and evolution of organic matter. Therefore, the inorganic pores in the Doushantuo Formation play a vital role in the enrichment and accumulation of shale gas. This study aims to establish a scientific basis for understanding the enrichment mechanism of shale gas in Doushantuo dolomitic shale and other inorganic pore-dominated shales in southern China.
Language	English
Publishing date	2024-02-08
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.3c08640
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Article ; Online: Early changes in cardiac troponin T and NT-proBNP levels in neonates receiving ECMO support: a single-center experience.

Xie, Wen-Peng / Liu, Yi-Nan / Zeng, Ya-Ting / Zheng, Yi-Rong / Chen, Qiang

BMC cardiovascular disorders

2024 Volume 24, Issue 1, Page(s) 233

Abstract: Objective: This study aimed to examine the changes in absolute value and decline rate of early serum cardiac troponin T (cTnT) and N-terminal pro b-type natriuretic peptide (NT-proBNP) in neonates who received veno-arterial (V-A) extracorporeal membrane ...

Abstract	Objective: This study aimed to examine the changes in absolute value and decline rate of early serum cardiac troponin T (cTnT) and N-terminal pro b-type natriuretic peptide (NT-proBNP) in neonates who received veno-arterial (V-A) extracorporeal membrane oxygenation (ECMO) support therapy within the first week of life. Methods: We retrospectively collected clinical data and laboratory test results of 18 neonates who underwent V-A ECMO support within one week of birth, from July 2021 to June 2023, using the electronic medical record system. These patients were categorized into survival and death groups. Comparative analyses of the absolute values and decline rates of cTnT and NT-proBNP were made between the groups at baseline, and at 24, 48, and 72 h post-ECMO initiation. Results: Out of the 18 neonates, 12 survived (survival rate: 66.7%), while 6 succumbed. The survival group exhibited significantly lower absolute values of cTnT and NT-proBNP than the death group, and their decline rates were significantly higher. Notably, all neonates without an early decline in cTnT and NT-proBNP levels were in the death group. Conclusion: The early changes in the absolute value and decline rate of serum cTnT and NT-proBNP in neonates undergoing V-A ECMO may serve as predictors of their prognosis.
MeSH term(s)	Humans ; Extracorporeal Membrane Oxygenation/adverse effects ; Extracorporeal Membrane Oxygenation/mortality ; Natriuretic Peptide, Brain/blood ; Troponin T/blood ; Infant, Newborn ; Peptide Fragments/blood ; Retrospective Studies ; Male ; Female ; Biomarkers/blood ; Time Factors ; Treatment Outcome ; Predictive Value of Tests ; Risk Factors
Chemical Substances	Natriuretic Peptide, Brain (114471-18-0) ; Troponin T ; pro-brain natriuretic peptide (1-76) ; Peptide Fragments ; Biomarkers
Language	English
Publishing date	2024-04-30
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2059859-2
ISSN	1471-2261 ; 1471-2261
ISSN (online)	1471-2261
ISSN	1471-2261
DOI	10.1186/s12872-024-03899-8
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Article ; Online: A Shape Memory Polymeric Shield for Protecting Corneal Endothelium During Phacoemulsification.

Liu, Yinan / Li, Yuqi / Ji, Jing / Fan, Yubo / Hong, Jing / Wang, Lizhen

Translational vision science & technology

2024 Volume 13, Issue 4, Page(s) 11

Abstract: Background: The purpose of this study was to explore the protective effect of a shape memory polymeric shield on corneal endothelium during phacoemulsification in rabbits.: Methods: Poly-(glycerol dodecanedioate) (PGD) with a transition temperature ... ...

Abstract	Background: The purpose of this study was to explore the protective effect of a shape memory polymeric shield on corneal endothelium during phacoemulsification in rabbits. Methods: Poly-(glycerol dodecanedioate) (PGD) with a transition temperature of 24.416°C was prepared to make a shape memory shield with a thickness of 100 µm, an arc length of 14 mm, and a radius of curvature of 8.8 mm. In the control group, a phaco-tip with bevel-down was used to simulate injury to the corneal endothelium by phacoemulsification in rabbits. In the experimental group, the pre-cooled and curled shape memory shield was injected into and removed from the anterior chamber before and after phaco-power release. Anterior segment optical coherence tomography (AS-OCT), confocal microscope, trypan blue/alizarin red staining, and scanning electron microscope were performed to measure endothelial damage after surgery. Results: One day postoperatively, the lost cell ratio of the control group and the experimental group were 28.08 ± 5.21% and 3.50 ± 1.43%, respectively (P < 0.0001), the damaged cell ratios were 11.83 ± 2.30% and 2.55 ± 0.52%, respectively (P < 0.0001), and the central corneal thicknesses (CCT) were 406.75 ± 16.74 µm and 340. 5 ±13.48 µm, respectively (P < 0.0001). Seven days postoperatively, the endothelial cell density (ECD) of the control group and the experimental group were 1674 ± 285/mm2 and 2561 ± 554/mm2, respectively (P < 0.05). The above differences were all statistically significant. Conclusions: This PGD based shape memory shield has a protective effect on corneal endothelium during phacoemulsification. It reduces postoperative corneal edema and ECD decrease in the short term after surgery. Translational relevance: The shape memory PGD "shield" in this study may have a use in certain human patients with vulnerable corneas of low endothelial cell count or shallow anterior chambers.
MeSH term(s)	Animals ; Humans ; Rabbits ; Endothelium, Corneal ; Phacoemulsification/adverse effects ; Phacoemulsification/methods ; Cornea ; Anterior Chamber
Language	English
Publishing date	2024-04-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2674602-5
ISSN	2164-2591 ; 2164-2591
ISSN (online)	2164-2591
ISSN	2164-2591
DOI	10.1167/tvst.13.4.11
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Article ; Online: Recent Advances in Manganese-Based Materials for Electrolytic Water Splitting.

Hu, Jing / Zhou, Yuru / Liu, Yinan / Xu, Zhichao / Li, Haijin

International journal of molecular sciences

2023 Volume 24, Issue 7

Abstract: Developing earth-abundant and highly effective electrocatalysts for electrocatalytic water splitting is a prerequisite for the upcoming hydrogen energy society. Recently, manganese-based materials have been one of the most promising candidates to replace ...

Abstract	Developing earth-abundant and highly effective electrocatalysts for electrocatalytic water splitting is a prerequisite for the upcoming hydrogen energy society. Recently, manganese-based materials have been one of the most promising candidates to replace noble metal catalysts due to their natural abundance, low cost, adjustable electronic properties, and excellent chemical stability. Although some achievements have been made in the past decades, their performance is still far lower than that of Pt. Therefore, further research is needed to improve the performance of manganese-based catalytic materials. In this review, we summarize the research progress on the application of manganese-based materials as catalysts for electrolytic water splitting. We first introduce the mechanism of electrocatalytic water decomposition using a manganese-based electrocatalyst. We then thoroughly discuss the optimization strategy used to enhance the catalytic activity of manganese-based electrocatalysts, including doping and defect engineering, interface engineering, and phase engineering. Finally, we present several future design opportunities for highly efficient manganese-based electrocatalysts.
MeSH term(s)	Manganese ; Catalysis ; Earth, Planet ; Electrolysis ; Electrolytes ; Water
Chemical Substances	Manganese (42Z2K6ZL8P) ; Electrolytes ; Water (059QF0KO0R)
Language	English
Publishing date	2023-04-06
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24076861
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Article ; Online: Establishment of plastic-associated microbial community from superworm gut microbiome.

Liu, Yi-Nan / Bairoliya, Sakcham / Zaiden, Norazean / Cao, Bin

Environment international

2023 Volume 183, Page(s) 108349

Abstract: Gut microbial communities of plastic-munching worms provide novel insights for the development of plastic-processing biotechnologies. Considering the complexity of worm maintenance and the gut microbial communities, it is challenging to apply the worms ... ...

Abstract	Gut microbial communities of plastic-munching worms provide novel insights for the development of plastic-processing biotechnologies. Considering the complexity of worm maintenance and the gut microbial communities, it is challenging to apply the worms directly in plastic processing. Harnessing the power of microbial communities derived from the worm gut microbiomes in vitro may enable a promising bioprocess for plastic degradation. Here, we established stable and reproducible plastic-associated biofilm communities derived from the gut microbiome of a superworm, Zophobas atratus, through a two-stage enrichment process: feeding with plastics (HDPE, PP, and PS) and in vitro incubation of gut microbiomes obtained from the plastic-fed worms. Plastic feeding exhibited marginal influence on bacterial diversity but substantially changed the relative abundance of different bacterial groups, and intriguingly, enriched potential plastic degraders. More prominent shifts of microbial communities were observed during the in vitro incubation of the gut microbiomes. Taxa containing plastic-degrading strains were further enriched, while other taxa represented by lactic acid bacteria were depleted. Additionally, the plastic characterization confirmed the degradation of the incubated plastics by the plastic-associated microbial communities. Community functional inference for both gene abundance and community phenotype suggested that the in vitro incubation enhanced plastic-degrading potential. Deterministic ecological effects, in particular, selection processes, were identified as the main driving force of the observed community shifts. Our findings provide novel insights into plastic-munching-worm-inspired bioprocessing of plastic wastes.
MeSH term(s)	Gastrointestinal Microbiome ; Microbiota ; Bacteria/genetics ; Biofilms ; Plastics
Chemical Substances	Plastics
Language	English
Publishing date	2023-11-23
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	554791-x
ISSN	1873-6750 ; 0160-4120
ISSN (online)	1873-6750
ISSN	0160-4120
DOI	10.1016/j.envint.2023.108349
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Article ; Online: The effect of reinforcement on the mechanical properties of veneered wood fiber/polypropylene composites assembled with chlorinated polypropylene.

Liu, Yinan / Chen, Feng / Ni, Xiaohui / Xia, Xinghua

Scientific reports

2022 Volume 12, Issue 1, Page(s) 14007

Abstract: Wood fiber/polypropylene (WF/PP) composites are environmentally friendly materials with high dimensional stability and mechanical properties. However, the applications of WF/PP composites are limited by an unattractive surface texture. In this study, the ...

Abstract	Wood fiber/polypropylene (WF/PP) composites are environmentally friendly materials with high dimensional stability and mechanical properties. However, the applications of WF/PP composites are limited by an unattractive surface texture. In this study, the WF/PP composites were decorated with poplar wood veneer using chlorinated polypropylene (CPP) as a hot melt adhesive, the bonding strength is over 1.18 MPa. Surface bonding strength tests and scanning electron microscopy (SEM) were performed to analyze the quality of the CPP bonding layer. The physical and mechanical properties of the veneered WF/PP boards and unveneered controls were examined to determine the effects of reinforcement. The result shows that after veneered the tension strength and modulus of the whole composite board were increased over 30% and 10%; the flexural strength and modulus were increased over 10% and 20%. Low-velocity impact testing was performed to determine the impact resistance properties of the composites. Higher ratios of wood fiber in the WF/PP composite led to a higher surface bonding strength, which was evident in the SEM images. Wood veneer decoration increased the mechanical strength of the whole composite board. A tighter bond along the CPP layer would provide additional reinforcement of the veneered composite's mechanical properties.
MeSH term(s)	Materials Testing ; Microscopy, Electron, Scanning ; Polypropylenes/chemistry ; Populus ; Wood/chemistry
Chemical Substances	Polypropylenes
Language	English
Publishing date	2022-08-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-17777-w
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