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Article ; Online: An Effective Alternative, Policy Experimentation, and the Multiple Streams Framework

Zhengbo Peng / Su Yang / Liying Dong / Jinhan Sun

SAGE Open, Vol

An Empirical Study of Chinese Rural Governance Policy Output

2024 Volume 14

Abstract: Although the Multiple Streams Framework (MSF) has abundant research, the mechanism of partial couplings to complete coupling remains to be investigated. The study extends the MSF using the perspective of generating effective alternatives in the policy ... ...

Abstract	Although the Multiple Streams Framework (MSF) has abundant research, the mechanism of partial couplings to complete coupling remains to be investigated. The study extends the MSF using the perspective of generating effective alternatives in the policy stream to explain the dynamic of the policy process. This article is an empirical study that compares two cases under the same holistic policy issues. We propose the multiple streams framework based on goal-oriented “policy solutions” to explain the process of Chinese rural governance policy output. The results show that: First, the partial coupling (politics and problems) opens the policy window for policy formulation, which drives the policy stream forming. Second, the periodic stability windows are a realistic path to accelerate the policy stream process. Third, policy experimentation constitutes an essential tool for formulating effective options. Further comparing the policy stream in the MSF with the policy stream in this study found clear differences.
Keywords	History of scholarship and learning. The humanities ; AZ20-999 ; Social Sciences ; H
Subject code	320
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	SAGE Publishing
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Two novel lncRNAs AF111167.2 and AL162377.1 targeting miR-21-5p mediated down expression of SYDE2 correlates with poor prognosis and tumor immune infiltration of ccRCC

Yuanshan Cui / Jitao Wu / Zhongbao Zhou / Jian Ma / Liying Dong

Heliyon, Vol 8, Iss 10, Pp e11079- (2022)

2022

Abstract: Advanced clear cell Renal Cell Carcinoma (ccRCC) is notoriously known for its poor prognosis. Synapse defective protein 1 homolog 2 encoded by the SYDE2 gene is a Rho GTPase-activating protein whose functional tumorigenic significance is still unclear. ... ...

Abstract	Advanced clear cell Renal Cell Carcinoma (ccRCC) is notoriously known for its poor prognosis. Synapse defective protein 1 homolog 2 encoded by the SYDE2 gene is a Rho GTPase-activating protein whose functional tumorigenic significance is still unclear. Recent pan-cancer analysis using the Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression (GTEx) data showed the potential tumor-suppressing effects of SYDE2 in ccRCC. Subsequently, the TCGA, GTEx data, and human protein atlas were employed to assess the correlation between the SYDE2 expression, clinical data, and overall survival (OS) in ccRCC patients. Furthermore, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) contributing to SYDE2 down expression were identified by expression, relationship, and survival analysis. Eventually, two novel lncRNAs, AL162377.1 and AF111167.2, targeting the miR-21-5p axis, were identified in the SYDE2 upstream non-coding RNAs (ncRNAs)-related pathway in ccRCC. The expression level of SYDE2 highly depends on the tumor immune cell infiltration and immune checkpoint expression. In summary, these data demonstrated that lncRNAs/miRNAs-mediated down-regulation of SYDE2 is related to the tumor immune infiltration. Hence, giving an insight into the prognosis of ccRCC.
Keywords	Clear cell renal cell carcinoma ; SYDE2 ; ncRNAs ; Prognosis ; Immune infiltrates ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
Subject code	616
Language	English
Publishing date	2022-10-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: New understanding of novel brominated flame retardants (NBFRs)

Liying Dong / Shutao Wang / Jinze Qu / Hong You / Dongmei Liu

Ecotoxicology and Environmental Safety, Vol 208, Iss , Pp 111570- (2021)

Neuro(endocrine) toxicity

2021

Abstract: Traditional brominated flame retardants (BFRs) negatively affect the environment and human health, especially in the sensitive (developing) nervous system. Considering the physicochemical similarities between novel brominated flame retardants (NBFRs) and ...

Abstract	Traditional brominated flame retardants (BFRs) negatively affect the environment and human health, especially in the sensitive (developing) nervous system. Considering the physicochemical similarities between novel brominated flame retardants (NBFRs) and BFRs, more and more evidence reveals the neurotoxic effects of NBFRs. We reviewed the neuro(endocrine) toxic effects of NBFRs in vivo and in vitro and discussed their action mechanisms based on the available information. The neurotoxic potential of NBFRs has been demonstrated through direct neurotoxicity and disruption of the neuroendocrine system, with adverse effects on neurobehavioral and reproductive development. Mechanistic studies have shown that the impact of NBFRs is related to the complex interaction of neural and endocrine signals. From disrupting the gender differentiation of the brain, altering serum thyroid/sex hormone levels, gene/protein expression, and so on, to interfere with the feedback effect between different levels of the HPG/HPT axis. In this paper, the mechanism of neurotoxic effects of NBFRs is explored from a new perspective-neuro and endocrine interactions. Gaps in the toxicity data of NBFRs in the neuroendocrine system are supplemented and provide a broader dataset for a complete risk assessment.
Keywords	Novel brominated flame retardants (NBFRs) ; Neurotoxicity ; Nervous system ; Thyroid/sex hormones ; Environmental pollution ; TD172-193.5 ; Environmental sciences ; GE1-350
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Identification of a Partial and Selective TRPV1 Agonist CPIPC for Alleviation of Inflammatory Pain

Liying Dong / Qiqi Zhou / Qianqian Liang / Zhen Qiao / Yani Liu / Liming Shao / Kewei Wang

Molecules, Vol 27, Iss 5428, p

2022 Volume 5428

Abstract: Transient receptor potential vanilloid 1 (TRPV1) is a non-selective cation channel, predominantly expressed in a subset of peripheral sensory neurons for pain signaling. Topical application of agonist capsaicin for desensitizing TRPV1 currents has been ... ...

Abstract	Transient receptor potential vanilloid 1 (TRPV1) is a non-selective cation channel, predominantly expressed in a subset of peripheral sensory neurons for pain signaling. Topical application of agonist capsaicin for desensitizing TRPV1 currents has been approved for relief of chronic pain. However, the potent TRPV1 capsaicin is not ingestible and even topical capsaicin causes common side effects such as skin irritation, swelling, erythema and pruritus, suggesting that a mild TRPV1 agonist might be helpful for reducing side effects while reliving pain. In this study, we reported on a partial and selective TRPV1 agonist 4-(5-chloropyridin-2-yl)- N -(1 H -indazol-6-yl)piperazine-1-carboxamide named CPIPC that was modified based on targeting the residue Arg557, important for conversion between the channel antagonism and agonism. Whole-cell patch clamp recordings indicated a concentration-dependent activation of TRPV1 currents by CPIPC with an EC 50 of 1.56 ± 0.13 μM. The maximum efficacy of CPIPC (30 μM) was about 60% of saturated capsaicin (10 μM). Repetitive additions of CPIPC caused TRPV1 current desensitization in both TRPV1-expressing HEK293 cells and dorsal root ganglion (DRG) sensory neurons. Oral administration of CPIPC dose-dependently alleviated inflammatory pain in mice. Further site-directed mutagenesis combined with molecular docking revealed that residue Arg557 is critical for TRPV1 activation by CPIPC . Taken together, we identified a novel partial and selective TRPV1 agonist CPIPC that exhibits antinociceptive activity in mice.
Keywords	TRPV ; desensitization ; whole-cell patch clamp ; site-directed mutagenesis ; dorsal root ganglion and antinociception ; Organic chemistry ; QD241-441
Subject code	500
Language	English
Publishing date	2022-08-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Deficient or R273H and R248W Mutations of p53 Promote Chemoresistance to 5-FU via TCF21/CD44 Axis-Mediated Enhanced Stemness in Colorectal Carcinoma

Xiaolei Gao / Xuan Zheng / Yixin Zhang / Liying Dong / Liangjie Sun / Na Zhao / Chong Ding / Zeyun Ma / Yixiang Wang

Frontiers in Cell and Developmental Biology, Vol

2022 Volume 9

Abstract: Background: p53 mutations are highly frequent in various human cancers and are reported to contribute to tumor malignance and chemoresistance. In this study, we explored the mechanism by which mutant p53 promotes carcinogenesis and chemoresistance and ... ...

Abstract	Background: p53 mutations are highly frequent in various human cancers and are reported to contribute to tumor malignance and chemoresistance. In this study, we explored the mechanism by which mutant p53 promotes carcinogenesis and chemoresistance and provided novel insights into cancer therapy.Materials and methods: A total of 409 patients with colorectal carcinoma from TCGA database were subdivided into two groups according to the p53 status, namely, mutant p53 and wild-type p53, following with GSEA analysis. The differences of the clinicopathologic index were also analyzed. Two HCT116 cell lines containing hot spots at codons R273H and R248W of p53 were constructed based on HCT116 with knockout p53, respectively. Cell viability, mobility, clonogenesis, and stemness were detected by CCK8, transwell migration and invasion, colonogenic, and sphere formation assays. Resistance to 5-FU was examined by live-dead staining and flow cytometry. qPCR, Western blot, and luciferase reporter assay were performed to identify that deficient or mutant p53 promoted chemoresistance of the colorectal carcinoma cell line HCT116 through the TCF21/CD44 signaling pathway, with the following rescue assays by overexpression of TCF21 and knockdown of CD44.Results: Patients with recurrence harbor a higher frequency of mutant p53 than those without recurrence (p < 0.05). The mutant p53 group developed a larger tumor than the wild-type one. GSEA analysis showed that oncogenic signatures were enriched in the mutant p53 group. Extracellular assays showed that cancer cells with deficient or mutant p53 (R273H and R248W, respectively) promoted colon cancer cell growth, migration, invasion, and stemness. The mutant cancer cells were also observed to be significantly resistant to 5-FU. Xenografts also confirmed that HCT116 cells harboring deficient or mutant p53 promoted cancer growth and 5-FU tolerance. Luciferase reporter assay showed that deficient or mutant p53 R237H and R248W endowed cancer cells with chemoresistance by activating CD44 ...
Keywords	colorectal carcinoma ; deficient or mutant p53 ; R273H ; R248W ; stemness ; TCF21/CD44 axis ; Biology (General) ; QH301-705.5
Subject code	572
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: miR-9-5p promotes myogenic differentiation via the Dlx3/Myf5 axis

Liying Dong / Meng Wang / Xiaolei Gao / Xuan Zheng / Yixin Zhang / Liangjie Sun / Na Zhao / Chong Ding / Zeyun Ma / Yixiang Wang

PeerJ, Vol 10, p e

2022 Volume 13360

Abstract: MicroRNAs play an important role in myogenic differentiation, they bind to target genes and regulate muscle formation. We previously found that miR-9-5p, which is related to bone formation, was increased over time during the process of myogenic ... ...

Abstract	MicroRNAs play an important role in myogenic differentiation, they bind to target genes and regulate muscle formation. We previously found that miR-9-5p, which is related to bone formation, was increased over time during the process of myogenic differentiation. However, the mechanism by which miR-9-5p regulates myogenic differentiation remains largely unknown. In the present study, we first examined myotube formation and miR-9-5p, myogenesis-related genes including Dlx3, Myod1, Mef2c, Desmin, MyoG and Myf5 expression under myogenic induction. Then, we detected the expression of myogenic transcription factors after overexpression or knockdown of miR-9-5p or Dlx3 in the mouse premyoblast cell line C2C12 by qPCR, western blot and myotube formation under myogenic induction. A luciferase assay was performed to confirm the regulatory relationships between not only miR-9-5p and Dlx3 but also Dlx3 and its downstream gene, Myf5, which is an essential transcription factor of myogenic differentiation. The results showed that miR-9-5p promoted myogenic differentiation by increasing myogenic transcription factor expression and promoting myotube formation, but Dlx3 exerted the opposite effect. Moreover, the luciferase assay showed that miR-9-5p bound to the 3’UTR of Dlx3 and downregulated Dlx3 expression. Dlx3 in turn suppressed Myf5 expression by binding to the Myf5 promoter, ultimately inhibiting the process of myogenic differentiation. In conclusion, the miR-9-5p/Dlx3/Myf5 axis is a novel pathway for the regulation of myogenic differentiation, and can be a potential target to treat the diseases related to muscle dysfunction.
Keywords	MiR-9-5p ; Dlx3 ; Myf5 ; Myogenic differentiation ; Medicine ; R ; Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	PeerJ Inc.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Marginal impact of cropping BADH transgenic maize BZ-136 on chemical property, enzyme activity, and bacterial community diversity of rhizosphere soil

Bai, Xin / Xing Zeng / Siqi Huang / Jinsong Liang / Liying Dong / Yingnan Wei / Yue Li / Juanjuan Qu / Zhenhua Wang

Plant and soil. 2019 Mar., v. 436, no. 1-2

2019

Abstract: AIMS: Transgenic betaine aldehyde dehydrogenase (BADH) maize that overaccumulates glycine betaine (GB) is developed to enhance tolerance to salt stress, while the ecological risk of cropping BADH transgenic maize BZ-136 on soil properties and rhizosphere ...

Abstract	AIMS: Transgenic betaine aldehyde dehydrogenase (BADH) maize that overaccumulates glycine betaine (GB) is developed to enhance tolerance to salt stress, while the ecological risk of cropping BADH transgenic maize BZ-136 on soil properties and rhizosphere microorganisms is ambiguous. METHODS: A pot experiment was conducted and soils were sampled at seedling, elongation, flowering, and mature stage. Soil chemical properties and enzyme activities were determined with conventional method and bacterial community diversity of BZ-136 rhizosphere was analyzed by high-throughput sequencing technique as compared with those of parental maize Zheng58 and conventional maize U8112. RESULTS: Cropping BZ-136 has a transient effect on EC, organic C or total N at some growth stage in neutral and saline-alkaline soil, a significant effect on urease activities from elongation to mature stage in saline-alkaline soil, and a slight influence on bacterial diversity at different stages in neutral soil and a significant impact at seedling stage in saline-alkaline soil. CONCLUSION: Cropping BADH transgenic maize has transient or minor effects on soil chemistry, enzyme activity, and bacterial community diversity, while parallel factors, such as plant growth stage and soil type might also influence the rhizosphere microorganisms.
Keywords	bacterial communities ; betaine ; betaine-aldehyde dehydrogenase ; corn ; enzyme activity ; flowering ; high-throughput nucleotide sequencing ; microorganisms ; plant growth ; rhizosphere ; risk ; salt stress ; salt tolerance ; seedlings ; soil chemical properties ; soil types ; transgenic plants ; urease
Language	English
Dates of publication	2019-03
Size	p. 527-541.
Publishing place	Springer International Publishing
Document type	Article
ZDB-ID	208908-7
ISSN	1573-5036 ; 0032-079X
ISSN (online)	1573-5036
ISSN	0032-079X
DOI	10.1007/s11104-019-03941-1
Database	NAL-Catalogue (AGRICOLA)

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Article: Inoculation of plant growth-promoting bacteria Bacillus sp. YM-1 alleviates the toxicity of Pb to pakchoi

Yu, Sumei / Jinsong Liang / Juanjuan Qu / Liying Dong / Siqi Huang / Xin Bai / Xuesheng Liu / Yingnan Wei / Yue Li

Environmental science and pollution research international. 2018 Oct., v. 25, no. 28

2018

Abstract: Heavy metal accumulation in plants may imperil human health. Inoculation of plant growth-promoting bacteria can alleviate the toxicity of heavy metal and promote plant growth. In this study, Bacillus sp. YM-1, a heavy metal resistant and plant growth- ... ...

Abstract	Heavy metal accumulation in plants may imperil human health. Inoculation of plant growth-promoting bacteria can alleviate the toxicity of heavy metal and promote plant growth. In this study, Bacillus sp. YM-1, a heavy metal resistant and plant growth-promoting bacterium, was immobilized with spent substrate of mushroom and applied to alleviate the toxicity of Pb to pakchoi. The results indicated that the biomass of pakchoi in inoculation group was increased by 17.45 to 27.05% compared with that in non-inoculated group (p < 0.05). The root and shoot were lengthened by 13.45% to 39.17% and 20.23% to 42.36%, respectively. The content of Pb in root and shoot obviously reduced and that in edible part (shoot) was less than 0.2 mg kg−1 in the low concentration of Pb. Other indicators such as superoxide dismutase (SOD), peroxidase (POD), chlorophyll, and protein all testified that YM-1 inoculation was conducive to the alleviation of Pb toxicity to pakchoi.
Keywords	Bacillus (bacteria) ; biomass ; Brassica rapa subsp. chinensis ; chlorophyll ; heavy metals ; human health ; lead ; metal tolerance ; mushrooms ; peroxidase ; plant growth ; plant growth-promoting rhizobacteria ; roots ; shoots ; superoxide dismutase ; toxicity
Language	English
Dates of publication	2018-10
Size	p. 28216-28225.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-018-2802-8
Database	NAL-Catalogue (AGRICOLA)

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Article: Composting enhances the removal of lead ions in aqueous solution by spent mushroom substrate: Biosorption and precipitation

Liu, Xuesheng / Jinsong Liang / Juanjuan Qu / Liying Dong / Siqi Huang / Xin Bai / Yingnan Wei / Yu Jin / Yue Li

Journal of cleaner production. 2018,

2018

Abstract: This study aims to elucidate the effects of composting on spent mushroom substrate (SMS) in lead ions removal by comparing the physicochemical properties of composted spent mushroom substrate (CSMS) with composted cow dung (CCD) and SMS. The effect of ... ...

Abstract	This study aims to elucidate the effects of composting on spent mushroom substrate (SMS) in lead ions removal by comparing the physicochemical properties of composted spent mushroom substrate (CSMS) with composted cow dung (CCD) and SMS. The effect of experimental factors (pH, contact time, dosage and initial concentration) on lead ions removal was determined by batch experiments. Composting elevated the pH value (7.80), cation exchange capacity (CEC, 77.02 cmol/kg) and hydrophilicity, resulting in a higher removal efficiency for lead ions of CSMS than that of SMS. The increased proportion of aromatic hydrocarbon and carboxyl was propitious to lead ions removal via their response to pH variation. Biosorption and precipitation were the overriding mechanisms involved in the lead ions removal by CSMS. Our results present a simple and energy-efficient method to enhance the ability of SMS to remove heavy metals.
Keywords	aqueous solutions ; biosorption ; cation exchange capacity ; cattle manure ; composting ; energy efficiency ; heavy metals ; hydrophilicity ; ions ; lead ; pH ; spent mushroom compost
Language	English
Size	p. .
Publishing place	Elsevier Ltd
Document type	Article
Note	Pre-press version
ISSN	0959-6526
DOI	10.1016/j.jclepro.2018.07.182
Database	NAL-Catalogue (AGRICOLA)

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Article: Characterization of siderophore produced by Pseudomonas syringae BAF.1 and its inhibitory effects on spore germination and mycelium morphology of Fusarium oxysporum

Yu, Sumei / Chunying Teng / Jinsong Liang / Tao Song / Liying Dong / Xin Bai / Yu Jin / Juanjuan Qu

journal of microbiology. 2017 Nov., v. 55, no. 11

2017

Abstract: In this study, an antagonistic bacterium against Fusarium oxysporum was identified and designated as Pseudomonas syringae strain BAF.1 on the basis of 16S rDNA sequence analysis and physiological-biochemical characteristics. It produced catechol-species ... ...

Abstract	In this study, an antagonistic bacterium against Fusarium oxysporum was identified and designated as Pseudomonas syringae strain BAF.1 on the basis of 16S rDNA sequence analysis and physiological-biochemical characteristics. It produced catechol-species siderophore at a molecular weight of 488.59 Da and a maximum amount of 55.27 μg/ml with glucose as a carbon source and asparagine as a nitrogen source at a C/N ratio of 10:1, 30°C and pH 7. The siderophore exhibited prominent antagonistic activity against Fusarium oxysporum with a maximum inhibition rate of 95.24% and had also suppressive effects on other kinds of 11 phytopathogenic fungi in the absence of FeCl₃·6H₂O. Spore germination was completely inhibited by 50 μl of the siderophorecontaining solution, and the ultrastructures of mycelia and spores were also considerably suppressed by siderophore treatment as established by electron microscopy observation. These results indicate that the siderophore produced by Pseudomonas syringae BAF.1 could be potentially used for biocontrol of pathogenic Fusarium oxysporum.
Keywords	Fusarium oxysporum ; Pseudomonas syringae ; asparagine ; bacteria ; biological control ; carbon ; carbon nitrogen ratio ; electron microscopy ; ferric chloride ; glucose ; molecular weight ; mycelium ; nitrogen ; nucleotide sequences ; pH ; plant pathogenic fungi ; ribosomal DNA ; sequence analysis ; siderophores ; spore germination ; spores ; ultrastructure
Language	English
Dates of publication	2017-11
Size	p. 877-884.
Publishing place	The Microbiological Society of Korea
Document type	Article
ZDB-ID	2012399-1
ISSN	1225-8873
ISSN	1225-8873
DOI	10.1007/s12275-017-7191-z
Database	NAL-Catalogue (AGRICOLA)

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