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  1. Article: In vitro

    Prišlin, Marina / Butorac, Ana / Bertoša, Rea / Kunić, Valentina / Ljolje, Ivana / Kostešić, Petar / Vlahović, Dunja / Naletilić, Šimun / Turk, Nenad / Brnić, Dragan

    Frontiers in veterinary science

    2024  Volume 11, Page(s) 1387174

    Abstract: Introduction: Canine adipose-derived mesenchymal stem cells (cAD-MSCs) hold therapeutic promise due to their regenerative potential, particularly within their secretome. However, concerns arise regarding the impact of : Methods: The study involved ... ...

    Abstract Introduction: Canine adipose-derived mesenchymal stem cells (cAD-MSCs) hold therapeutic promise due to their regenerative potential, particularly within their secretome. However, concerns arise regarding the impact of
    Methods: The study involved collecting abdominal adipose tissue samples from nine healthy female dogs, from which cAD-MSCs were extracted and cultured. Stem cells were validated through trilineage differentiation assays and flow cytometry immunophenotyping. Gene expression profiling using RT-qPCR array, and cAD-MSCs secretome LC-MS/MS analysis, were conducted at passages 3 and 6 to reveal gene expression and protein composition alterations during
    Results and discussion: The results demonstrate that the gene expression and secretome composition of cAD-MSCs were impacted by
    Language English
    Publishing date 2024-03-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2024.1387174
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: An Outstanding Role of Adipose Tissue in Canine Stem Cell Therapy.

    Prišlin, Marina / Vlahović, Dunja / Kostešić, Petar / Ljolje, Ivana / Brnić, Dragan / Turk, Nenad / Lojkić, Ivana / Kunić, Valentina / Karadjole, Tugomir / Krešić, Nina

    Animals : an open access journal from MDPI

    2022  Volume 12, Issue 9

    Abstract: Adipose tissue, previously known as connective tissue with a role in energy storage, is currently changing the course of treatments in veterinary medicine. Recent studies have revealed one particularly impressive function among all the newly discovered ... ...

    Abstract Adipose tissue, previously known as connective tissue with a role in energy storage, is currently changing the course of treatments in veterinary medicine. Recent studies have revealed one particularly impressive function among all the newly discovered functions of adipose tissue. The interactive cells hosted by adipose tissue, the stromal vascular fraction (SVF), and their role in treating numerous diseases have provided a prospective course of research with positive outcomes in regenerative veterinary medicine (RVM). This review describes the main features of adipose tissue, emphasizing an eclectic combination of cells within the SVF and its thus far researched therapeutic possibilities in canine RVM. An afterwards focus is on a highly researched component of the SVF, adipose-derived mesenchymal stem cells (ASCs), which were shown to have an extraordinary impact relying on several proposed mechanisms of action on mitigating pathologies in canines. Furthermore, ASC therapy showed the most significant results in the orthopaedics field and in neurology, dermatology, ophthalmology, gastroenterology, and hepatology, which elevates the possibilities of ASC therapy to a whole new level. Therefore, this review article aims to raise awareness of the importance of research on cellular components, within abundant and easily accessible adipose tissue, in the direction of regenerative therapy in canines, considering the positive outcomes so far. Although the focus is on the positive aspects of cellular therapy in canines, the researchers should not forget the importance of identifying the potential negative aspects within published and upcoming research. Safe and standardized treatment represents a fundamental prerequisite for positively impacting the lives of canine patients.
    Language English
    Publishing date 2022-04-22
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani12091088
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The Expression Pattern of Surface Markers in Canine Adipose-Derived Mesenchymal Stem Cells.

    Krešić, Nina / Prišlin, Marina / Vlahović, Dunja / Kostešić, Petar / Ljolje, Ivana / Brnić, Dragan / Turk, Nenad / Musulin, Andrija / Habrun, Boris

    International journal of molecular sciences

    2021  Volume 22, Issue 14

    Abstract: The influence of cultivation on the expression pattern of canine adipose-derived mesenchymal stem cells (cAD-MSCs) surface markers, contributing to, among others, the promotion of growth, proliferation, differentiation and immunomodulatory mechanisms of ... ...

    Abstract The influence of cultivation on the expression pattern of canine adipose-derived mesenchymal stem cells (cAD-MSCs) surface markers, contributing to, among others, the promotion of growth, proliferation, differentiation and immunomodulatory mechanisms of an excellent therapeutic, is still unknown. To fill the gap, we investigated CD90, CD44, CD73, CD29, CD271, CD105, CD45 and CD14 patterns of expression at the protein level with flow cytometry and mRNA level using a real-time polymerase chain reaction array. Gentle variations of expression occurred during cultivation, along with increased CD90, CD44 and CD29 expression, low and decreasing CD271 and CD73 expression and a decrease of initially high CD105. As expected, CD45 and CD14 were not expressed by cAD-MSCs. Interestingly, we discovered a significant decrease of CD73 expression, compared to early (P1-P3) to late (P4-P6) passages, although the CD73 gene expression was found to be stable. The percentage of positive cells was found to be higher for all positive markers up to P4. As CD73's one important feature is a modulation from a pro-inflammatory environment to an anti-inflammatory milieu, the expression of CD73 in our conditions indicate the need to consider the time cells spend in vitro before being transplanted into patients, since it could impact their favourable therapeutical properties.
    MeSH term(s) Animals ; Biomarkers/metabolism ; Cell Differentiation ; Dogs ; Female ; Gene Expression Profiling ; Gene Expression Regulation ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/metabolism
    Chemical Substances Biomarkers ; Membrane Proteins
    Language English
    Publishing date 2021-07-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22147476
    Database MEDical Literature Analysis and Retrieval System OnLINE

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