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  1. Article ; Online: Structural characterization of a homopolysaccharide produced by Weissella cibaria FMy 2-21-1 and its potential application as a green corrosion inhibiting film.

    Lobo, René Emanuel / Orrillo, Patricio Andrés / Ribotta, Susana Beatriz / de Valdez, Graciela Font / García, Mercedes Santos / Cabello, José Carlos Rodríguez / Torino, María Inés

    International journal of biological macromolecules

    2022  Volume 212, Page(s) 193–201

    Abstract: Steel corrosion is a global issue that affects safety and the economy. Currently, the homopolysaccharide (HoPS) structure of a novel lactic acid bacterium (LAB) is under study, as well as its application as a green corrosion inhibitor. Weissella cibaria ... ...

    Abstract Steel corrosion is a global issue that affects safety and the economy. Currently, the homopolysaccharide (HoPS) structure of a novel lactic acid bacterium (LAB) is under study, as well as its application as a green corrosion inhibitor. Weissella cibaria FMy 2-21-1 is a LAB strain capable of producing HoPS in sucrose enriched media. The isolated and purified HoPS was characterized by different spectroscopic analyses as a linear α-1,6 dextran adopting a random coil conformation, with high molecular weight and extended size in water. The polysaccharide showed a semi-crystalline organization, which is a requirement for film formation. Its biocoating showed a grainy network structure, with a slightly lesser hydrophobic role in the aqueous environment than in the ionic one. The electrochemical measurements of the steel-HoPS coating showed that the biopolymer layer acts as an anodic-type corrosion inhibitor, with high resistance to corrosion by water and with chloride ions which prevent pitting, a corrosion process typical of bare steel. Few reports have cited the application of LAB HoPS as corrosive coating inhibitors. This work is the first to explore the influence of a structurally characterized dextran from Weissella cibaria strain as a potential steel corrosion inhibitor in ionic environments.
    MeSH term(s) Corrosion ; Dextrans/chemistry ; Steel/chemistry ; Water ; Weissella/chemistry
    Chemical Substances Dextrans ; Water (059QF0KO0R) ; Steel (12597-69-2)
    Language English
    Publishing date 2022-05-18
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2022.05.105
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Structural characterization of a homopolysaccharide produced by Weissella cibaria FMy 2-21-1 and its potential application as a green corrosion inhibiting film

    Lobo, René Emanuel / Orrillo, Patricio Andrés / Ribotta, Susana Beatriz / de Valdez, Graciela Font / García, Mercedes Santos / Cabello, José Carlos Rodríguez / Torino, María Inés

    International journal of biological macromolecules. 2022 July 01, v. 212

    2022  

    Abstract: Steel corrosion is a global issue that affects safety and the economy. Currently, the homopolysaccharide (HoPS) structure of a novel lactic acid bacterium (LAB) is under study, as well as its application as a green corrosion inhibitor. Weissella cibaria ... ...

    Abstract Steel corrosion is a global issue that affects safety and the economy. Currently, the homopolysaccharide (HoPS) structure of a novel lactic acid bacterium (LAB) is under study, as well as its application as a green corrosion inhibitor. Weissella cibaria FMy 2-21-1 is a LAB strain capable of producing HoPS in sucrose enriched media. The isolated and purified HoPS was characterized by different spectroscopic analyses as a linear α-1,6 dextran adopting a random coil conformation, with high molecular weight and extended size in water. The polysaccharide showed a semi-crystalline organization, which is a requirement for film formation. Its biocoating showed a grainy network structure, with a slightly lesser hydrophobic role in the aqueous environment than in the ionic one. The electrochemical measurements of the steel-HoPS coating showed that the biopolymer layer acts as an anodic-type corrosion inhibitor, with high resistance to corrosion by water and with chloride ions which prevent pitting, a corrosion process typical of bare steel. Few reports have cited the application of LAB HoPS as corrosive coating inhibitors. This work is the first to explore the influence of a structurally characterized dextran from Weissella cibaria strain as a potential steel corrosion inhibitor in ionic environments.
    Keywords Weissella cibaria ; biopolymers ; corrosion ; dextran ; electrochemistry ; hydrophobicity ; lactic acid bacteria ; molecular weight ; spectroscopy ; steel ; sucrose
    Language English
    Dates of publication 2022-0701
    Size p. 193-201.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2022.05.105
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Partial Characterization and Immunomodulatory Effects of Exopolysaccharides from Streptococcus thermophilus SBC8781 during Soy Milk and Cow Milk Fermentation

    Nakata, Hajime / Imamura, Yoshiya / Saha, Sudeb / Lobo, René Emanuel / Kitahara, Shugo / Araki, Shota / Tomokiyo, Mikado / Namai, Fu / Hiramitsu, Masanori / Inoue, Takashi / Nishiyama, Keita / Villena, Julio / Kitazawa, Haruki

    Foods. 2023 June 15, v. 12, no. 12

    2023  

    Abstract: The immunomodulatory properties of exopolysaccharides (EPSs) produced by Streptococcus thermophilus have not been explored in depth. In addition, there are no comparative studies of the functional properties of EPSs produced by streptococci in different ... ...

    Abstract The immunomodulatory properties of exopolysaccharides (EPSs) produced by Streptococcus thermophilus have not been explored in depth. In addition, there are no comparative studies of the functional properties of EPSs produced by streptococci in different food matrices. In this work, EPSs from S. thermophilus SBC8781 were isolated after soy milk (EPS-s) or cow milk (EPS-m) fermentation, identified, and characterized in their abilities to modulate immunity in porcine intestinal epithelial cells. Fresh soy milk and cow milk were inoculated with S. thermophilus SBC8781 (7 log CFU/mL) and incubated at 37 °C for 24 h. The extraction of EPSs was performed by the ethanol precipitation method. Analytical techniques, including NMR, UV-vis spectroscopy, and chromatography, identified and characterized both biopolymer samples as polysaccharides with high purity levels and similar Mw. EPS-s and EPS-m had heteropolysaccharide structures formed by galactose, glucose, rhamnose, ribose, and mannose, although with different monomer proportions. On the other hand, EPS-s had higher quantities of acidic polymer than EPS-m. The biopolymer production of the SBC8781 strain from the vegetable culture broth was 200–240 mg/L, which was higher than that produced in milk, which reached concentrations of 50–70 mg/L. For immunomodulatory assays, intestinal epithelial cells were stimulated with 100 µg/mL of EPS-s or EPS-m for 48 h and then stimulated with the Toll-like receptor 3 agonist poly(I:C). EPS-s significantly reduced the expression of IL-6, IFN-β, IL-8, and MCP-1 and increased the negative regulator A20 in intestinal epithelial cells. Similarly, EPS-m induced a significant reduction of IL-6 and IL-8 expressions, but its effect was less remarkable than that caused by EPS-s. Results indicate that the structure and the immunomodulatory activity of EPSs produced by the SBC8781 strain vary according to the fermentation substrate. Soy milk fermented with S. thermophilus SBC8781 could be a new immunomodulatory functional food, which should be further evaluated in preclinical trials.
    Keywords Streptococcus thermophilus ; Toll-like receptor 3 ; agonists ; biopolymers ; chromatography ; culture media ; epithelium ; ethanol ; exopolysaccharides ; fermentation ; functional foods ; galactose ; glucose ; immunity ; immunomodulators ; interleukin-6 ; interleukin-8 ; intestines ; mannose ; milk ; rhamnose ; ribose ; soymilk ; swine ; ultraviolet-visible spectroscopy ; vegetable growing
    Language English
    Dates of publication 2023-0615
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12122374
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Partial Characterization and Immunomodulatory Effects of Exopolysaccharides from

    Nakata, Hajime / Imamura, Yoshiya / Saha, Sudeb / Lobo, René Emanuel / Kitahara, Shugo / Araki, Shota / Tomokiyo, Mikado / Namai, Fu / Hiramitsu, Masanori / Inoue, Takashi / Nishiyama, Keita / Villena, Julio / Kitazawa, Haruki

    Foods (Basel, Switzerland)

    2023  Volume 12, Issue 12

    Abstract: The immunomodulatory properties of exopolysaccharides (EPSs) produced ... ...

    Abstract The immunomodulatory properties of exopolysaccharides (EPSs) produced by
    Language English
    Publishing date 2023-06-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12122374
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Physicochemical and antioxidant properties of a gastroprotective exopolysaccharide produced by Streptococcus thermophilus CRL1190

    Lobo, René Emanuel / Gómez, María Inés / Font de Valdez, Graciela / Torino, María Inés

    Food hydrocolloids. 2019 Nov., v. 96

    2019  

    Abstract: The exopolysaccharide EPS1190 was isolated with high purity from skim milk fermented by the strain Streptococcus thermophilus CRL1190, the major EPS amount being recovered at stationary growth-phase (70.7 mg/L after 16 h). This polymer revealed moderate ... ...

    Abstract The exopolysaccharide EPS1190 was isolated with high purity from skim milk fermented by the strain Streptococcus thermophilus CRL1190, the major EPS amount being recovered at stationary growth-phase (70.7 mg/L after 16 h). This polymer revealed moderate antioxidant activity in vitro and useful technological properties such as good emulsifying and flocculating capacity at low concentrations, high aqueous solubility (9.1 mg/mL), water and oil holding capacity (528.5% and 1266%, respectively) as well as thermal behavior. Thermogravimetric, Differential Thermal Analysis and Differential Scanning Calorimetry curves showed that the biopolymer was thermally stable up to 216 °C with a crystalline deformation in an endothermic process (△H = 284.46 J) at a melting temperature of 74.08 °C. Furthermore, a degradation temperature (Td) of 295.4 °C was observed. These results postulate that EPS1190 could be used in the food industry due to its special micro-structure (scanning electron microscopy of EPS1190 demonstrated a homogeneous matrix with micro-porous and rough structure), its good performance and functionality in both aqueous and oil systems, which would remain at high temperatures.
    Keywords Streptococcus thermophilus ; antioxidant activity ; biopolymers ; deformation ; developmental stages ; differential scanning calorimetry ; differential thermal analysis ; emulsifying ; endothermy ; exopolysaccharides ; fermented milk ; food industry ; hydrocolloids ; melting point ; microstructure ; oils ; scanning electron microscopy ; skim milk ; solubility ; temperature ; thermal stability ; thermogravimetry
    Language English
    Dates of publication 2019-11
    Size p. 625-633.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 742742-6
    ISSN 1873-7137 ; 0268-005X
    ISSN (online) 1873-7137
    ISSN 0268-005X
    DOI 10.1016/j.foodhyd.2019.05.036
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Techno-functional properties of HoPS from lactic acid bacteria of different origins as potential food additives.

    Lobo, René Emanuel / Figueroa, Theo / Navarro, Diego / Gómez, María Inés / Font de Valdez, Graciela / Torino, María Inés

    Food chemistry

    2021  Volume 356, Page(s) 129627

    Abstract: Homopolysaccharides (HoPS) produced by lactic acid bacteria (LAB) are highly versatile, biocompatible and safe compounds. In this work, six HoPS from different species of Weisella and Leuconostoc were identified as thermally stable dextrans, with ... ...

    Abstract Homopolysaccharides (HoPS) produced by lactic acid bacteria (LAB) are highly versatile, biocompatible and safe compounds. In this work, six HoPS from different species of Weisella and Leuconostoc were identified as thermally stable dextrans, with endothermic crystalline deformations between 214 and 239 °C. These dextrans proved to have greater solubility and capacities to retain water and oil than similar polymers in other reports. Furthermore, a surface morphology study presented cubic grumps, stratify mesh with irregular grumps, and highly compact filaments. Assays in vitro revealed moderate antioxidant, browning and foaming activities as well as technological properties, such as anti-syneresis, emulsifying and flocculating activities, even at low concentrations. Taking into account bipolymers' microstructure, functionalities and performance in both, aqueous and hydrophobic matrixes, plus their capacity to maintain themselves at elevated temperatures, we consider these HoPS beneficial and natural food additives.
    MeSH term(s) Emulsions ; Food Additives/chemistry ; Food Additives/metabolism ; Hydrophobic and Hydrophilic Interactions ; Lactobacillales/chemistry ; Lactobacillales/metabolism ; Polysaccharides/chemistry ; Solubility ; Water/chemistry
    Chemical Substances Emulsions ; Food Additives ; Polysaccharides ; Water (059QF0KO0R)
    Language English
    Publishing date 2021-03-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2021.129627
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Physicochemical and antioxidant properties of a gastroprotective exopolysaccharide produced by Streptococcus thermophilic CRL1190

    Lobo, René Emanuel / Gómez, Maria Inés / Font de Valdez, Graciela / Torino, Maria Inés

    Food hydrocolloids

    2019  Volume 96, Issue -, Page(s) 625–633

    Language English
    Document type Article
    ZDB-ID 742742-6
    ISSN 0268-005X
    Database Current Contents Nutrition, Environment, Agriculture

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