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  1. Article ; Online: IL-10-Engineered Human CD4

    Locafaro, Grazia / Andolfi, Grazia / Russo, Fabio / Cesana, Luca / Spinelli, Antonello / Camisa, Barbara / Ciceri, Fabio / Lombardo, Angelo / Bondanza, Attilio / Roncarolo, Maria Grazia / Gregori, Silvia

    Molecular therapy : the journal of the American Society of Gene Therapy

    2024  Volume 32, Issue 3, Page(s) 853–854

    Language English
    Publishing date 2024-02-14
    Publishing country United States
    Document type Published Erratum
    ZDB-ID 2010592-7
    ISSN 1525-0024 ; 1525-0016
    ISSN (online) 1525-0024
    ISSN 1525-0016
    DOI 10.1016/j.ymthe.2024.02.012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5640: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: HLA-G expression on blasts and tolerogenic cells in patients affected by acute myeloid leukemia.

    Locafaro, Grazia / Amodio, Giada / Tomasoni, Daniela / Tresoldi, Cristina / Ciceri, Fabio / Gregori, Silvia

    Journal of immunology research

    2014  Volume 2014, Page(s) 636292

    Abstract: Human Leukocyte Antigen-G (HLA-G) contributes to cancer cell immune escape from host antitumor responses. The clinical relevance of HLA-G in several malignancies has been reported. However, the role of HLA-G expression and functions in Acute Myeloid ... ...

    Abstract Human Leukocyte Antigen-G (HLA-G) contributes to cancer cell immune escape from host antitumor responses. The clinical relevance of HLA-G in several malignancies has been reported. However, the role of HLA-G expression and functions in Acute Myeloid Leukemia (AML) is still controversial. Our group identified a subset of tolerogenic dendritic cells, DC-10 that express HLA-G and secrete IL-10. DC-10 are present in the peripheral blood and are essential in promoting and maintaining tolerance via the induction of adaptive T regulatory (Treg) cells. We investigated HLA-G expression on blasts and the presence of HLA-G-expressing DC-10 and CD4(+) T cells in the peripheral blood of AML patients at diagnosis. Moreover, we explored the possible influence of the 3' untranslated region (3'UTR) of HLA-G, which has been associated with HLA-G expression, on AML susceptibility. Results showed that HLA-G-expressing DC-10 and CD4(+) T cells are highly represented in AML patients with HLA-G positive blasts. None of the HLA-G variation sites evaluated was associated with AML susceptibility. This is the first report describing HLA-G-expressing DC-10 and CD4(+) T cells in AML patients, suggesting that they may represent a strategy by which leukemic cells escape the host's immune system. Further studies on larger populations are required to verify our findings.
    MeSH term(s) 3' Untranslated Regions ; Adult ; Aged ; Aged, 80 and over ; Dendritic Cells/immunology ; Dendritic Cells/pathology ; Disease Susceptibility ; Female ; Gene Expression/immunology ; HLA-G Antigens/genetics ; HLA-G Antigens/immunology ; Humans ; Immune Evasion ; Immune Tolerance ; Interleukin-10/immunology ; Interleukin-10/metabolism ; Leukemia, Myeloid, Acute/genetics ; Leukemia, Myeloid, Acute/immunology ; Leukemia, Myeloid, Acute/pathology ; Male ; Middle Aged ; T-Lymphocytes, Regulatory/immunology ; T-Lymphocytes, Regulatory/pathology
    Chemical Substances 3' Untranslated Regions ; HLA-G Antigens ; IL10 protein, human ; Interleukin-10 (130068-27-8)
    Language English
    Publishing date 2014-03-13
    Publishing country Egypt
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2817541-4
    ISSN 2314-7156 ; 2314-7156
    ISSN (online) 2314-7156
    ISSN 2314-7156
    DOI 10.1155/2014/636292
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: IL-10-Engineered Human CD4

    Locafaro, Grazia / Andolfi, Grazia / Russo, Fabio / Cesana, Luca / Spinelli, Antonello / Camisa, Barbara / Ciceri, Fabio / Lombardo, Angelo / Bondanza, Attilio / Roncarolo, Maria Grazia / Gregori, Silvia

    Molecular therapy : the journal of the American Society of Gene Therapy

    2017  Volume 25, Issue 10, Page(s) 2254–2269

    Abstract: T regulatory cells (Tregs) play a key role in modulating T cell responses. Clinical trials showed that Tregs modulate graft-versus-host disease (GvHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, their ability to mediate ...

    Abstract T regulatory cells (Tregs) play a key role in modulating T cell responses. Clinical trials showed that Tregs modulate graft-versus-host disease (GvHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, their ability to mediate anti-leukemic activity (graft-versus-leukemia [GvL]) is largely unknown. Enforced interleukin-10 (IL-10) expression converts human CD4
    MeSH term(s) CD4-Positive T-Lymphocytes ; Humans ; Immunotherapy ; Interleukin-10/metabolism ; Leukemia, Myeloid/immunology ; Leukemia, Myeloid/metabolism ; Leukemia, Myeloid/therapy ; Leukocytes, Mononuclear/metabolism ; Models, Biological ; T-Lymphocytes, Regulatory/metabolism
    Chemical Substances Interleukin-10 (130068-27-8)
    Language English
    Publishing date 2017-07-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2010592-7
    ISSN 1525-0024 ; 1525-0016
    ISSN (online) 1525-0024
    ISSN 1525-0016
    DOI 10.1016/j.ymthe.2017.06.029
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5640: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Corrigendum: Monitoring T-Cell Responses in Translational Studies: Optimization of Dye-Based Proliferation Assay for Evaluation of Antigen-Specific Responses.

    Ten Brinke, Anja / Marek-Trzonkowska, Natalia / Mansilla, Maria J / Turksma, Annelies W / Piekarska, Karolina / Iwaszkiewicz-Grześ, Dorota / Passerini, Laura / Locafaro, Grazia / Puñet-Ortiz, Joan / van Ham, S Marieke / Hernandez-Fuentes, Maria P / Martínez-Cáceres, Eva M / Gregori, Silvia

    Frontiers in immunology

    2018  Volume 9, Page(s) 343

    Abstract: This corrects the article on p. 1870 in vol. 8, PMID: 29312346.]. ...

    Abstract [This corrects the article on p. 1870 in vol. 8, PMID: 29312346.].
    Language English
    Publishing date 2018-02-22
    Publishing country Switzerland
    Document type Journal Article ; Published Erratum
    ZDB-ID 2606827-8
    ISSN 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2018.00343
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Enforced IL-10 expression confers type 1 regulatory T cell (Tr1) phenotype and function to human CD4(+) T cells.

    Andolfi, Grazia / Fousteri, Georgia / Rossetti, Maura / Magnani, Chiara F / Jofra, Tatiana / Locafaro, Grazia / Bondanza, Attilio / Gregori, Silvia / Roncarolo, Maria-Grazia

    Molecular therapy : the journal of the American Society of Gene Therapy

    2012  Volume 20, Issue 9, Page(s) 1778–1790

    Abstract: Type 1 regulatory T (Tr1) cells are an inducible subset of CD4(+) Tr cells characterized by high levels of interleukin (IL)-10 production and regulatory properties. Several protocols to generate human Tr1 cells have been developed in vitro. However, the ... ...

    Abstract Type 1 regulatory T (Tr1) cells are an inducible subset of CD4(+) Tr cells characterized by high levels of interleukin (IL)-10 production and regulatory properties. Several protocols to generate human Tr1 cells have been developed in vitro. However, the resulting population includes a significant fraction of contaminating non-Tr1 cells, representing a major bottleneck for clinical application of Tr1 cell therapy. We generated an homogeneous IL-10-producing Tr1 cell population by transducing human CD4(+) T cells with a bidirectional lentiviral vector (LV) encoding for human IL-10 and the marker gene, green fluorescent protein (GFP), which are independently coexpressed. The resulting GFP(+) LV-IL-10-transduced human CD4(+) T (CD4(LV-IL-10)) cells expressed, upon T-cell receptor (TCR) activation, high levels of IL-10 and concomitant low levels of IL-4, and markers associated with IL-10. Moreover, CD4(LV-IL-10) T cells displayed typical Tr1 features: the anergic phenotype, the IL-10, and transforming growth factor (TGF)-β dependent suppression of allogeneic T-cell responses, and the ability to suppress in a cell-to-cell contact independent manner in vitro. CD4(LV-IL-10) T cells were able to control xeno graft-versus-host disease (GvHD), demonstrating their suppressive function in vivo. These results show that constitutive over-expression of IL-10 in human CD4(+) T cells leads to a stable cell population that recapitulates the phenotype and function of Tr1 cells.
    MeSH term(s) Animals ; Cell Differentiation ; Clonal Anergy ; Dendritic Cells/cytology ; Dendritic Cells/immunology ; Female ; Gene Expression ; Genes, Reporter ; Genetic Vectors ; Graft vs Host Disease/immunology ; Graft vs Host Disease/prevention & control ; Green Fluorescent Proteins ; Humans ; Immunomodulation ; Interleukin-10/biosynthesis ; Interleukin-10/genetics ; Interleukin-10/immunology ; Interleukin-4/immunology ; Lentivirus/genetics ; Lymphocyte Activation/immunology ; Mice ; Mice, Nude ; T-Lymphocytes, Regulatory/cytology ; T-Lymphocytes, Regulatory/immunology ; T-Lymphocytes, Regulatory/transplantation ; Transduction, Genetic
    Chemical Substances Interleukin-10 (130068-27-8) ; Green Fluorescent Proteins (147336-22-9) ; Interleukin-4 (207137-56-2)
    Language English
    Publishing date 2012-06-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2010592-7
    ISSN 1525-0024 ; 1525-0016
    ISSN (online) 1525-0024
    ISSN 1525-0016
    DOI 10.1038/mt.2012.71
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5640: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article: Monitoring T-Cell Responses in Translational Studies: Optimization of Dye-Based Proliferation Assay for Evaluation of Antigen-Specific Responses.

    Ten Brinke, Anja / Marek-Trzonkowska, Natalia / Mansilla, Maria J / Turksma, Annelies W / Piekarska, Karolina / Iwaszkiewicz-Grześ, Dorota / Passerini, Laura / Locafaro, Grazia / Puñet-Ortiz, Joan / van Ham, S Marieke / Hernandez-Fuentes, Maria P / Martínez-Cáceres, Eva M / Gregori, Silvia

    Frontiers in immunology

    2017  Volume 8, Page(s) 1870

    Abstract: Adoptive therapy with regulatory T cells or tolerance-inducing antigen (Ag)-presenting cells is innovative and promising therapeutic approach to control undesired and harmful activation of the immune system, as observed in autoimmune diseases, solid ... ...

    Abstract Adoptive therapy with regulatory T cells or tolerance-inducing antigen (Ag)-presenting cells is innovative and promising therapeutic approach to control undesired and harmful activation of the immune system, as observed in autoimmune diseases, solid organ and bone marrow transplantation. One of the critical issues to elucidate the mechanisms responsible for success or failure of these therapies and define the specificity of the therapy is the evaluation of the Ag-specific T-cell responses. Several efforts have been made to develop suitable and reproducible assays. Here, we focus on dye-based proliferation assays. We highlight with practical examples the fundamental issues to take into consideration for implementation of an effective and sensitive dye-based proliferation assay to monitor Ag-specific responses in patients. The most critical points were used to design a road map to set up and analyze the optimal assay to assess Ag-specific T-cell responses in patients undergoing different treatments. This is the first step to optimize monitoring of tolerance induction, allowing comparison of outcomes of different clinical studies. The road map can also be applied to other therapeutic interventions, not limited to tolerance induction therapies, in which Ag-specific T-cell responses are relevant such as vaccination approaches and cancer immunotherapy.
    Language English
    Publishing date 2017-12-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606827-8
    ISSN 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2017.01870
    Database MEDical Literature Analysis and Retrieval System OnLINE

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