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Article: Circovirus et pathologies associées chez les animaux.

Grasland, B / Bigarré, L / Blanchard, P / Loizel, C / Blanchard, Y / de Boisséson, C / Jestin, A

2021 Volume 9, Issue 6, Page(s) 431–442

Abstract: Animal circoviruses belong to the Circovirus genus of the Circoviridae family. Nowadays, only swine and birds were identified as circovirus hosts. Circoviruses have a single-stranded circular genome of approximately 2000 nucleotide long. DNA of these ... ...

Title translation	Animal circoviruses and associated diseases.
Abstract	Animal circoviruses belong to the Circovirus genus of the Circoviridae family. Nowadays, only swine and birds were identified as circovirus hosts. Circoviruses have a single-stranded circular genome of approximately 2000 nucleotide long. DNA of these viruses possesses : (i) a nonanucleotide sequence essential for replication, flanked by inverted repeat sequences, a palindrome that has the potential to form a stem-loop structure and (ii) two major ORFs, located on the viral and complementary strands, which encode respectively the replication-associated protein (Rep) and the capsid protein (Cap). All the circoviruses described at the present time, except porcine circovirus of type 1, are associated with immunosuppressive or immunodepressive diseases. Histopathological lesions such as cytoplasmic inclusions of virus in histiocytic cells and T and B lymphocyte depletion in lymphoid organs, are commonly noticed. No medical prophylaxis of circovirus infections is currently available.
Language	French
Publishing date	2021-10-11
Publishing country	France
Document type	English Abstract ; Journal Article
ZDB-ID	2118387-9
ISSN	1950-6961 ; 1267-8694
ISSN (online)	1950-6961
ISSN	1267-8694
DOI	10.1684/vir.2011.2807
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Article: Development of genetic tools for Lactobacillus sakei: disruption of the beta-galactosidase gene and use of lacZ as a reporter gene To study regulation of the putative copper ATPase, AtkB.

Stentz, R / Loizel, C / Malleret, C / Zagorec, M

Applied and environmental microbiology

2000 Volume 66, Issue 10, Page(s) 4272–4278

Abstract: Downstream from the ptsHI operon of Lactobacillus sakei, the genes atkY and atkB, organized in an operon, were observed. The two putative proteins, AtkB and AtkY, show sequence similarity to the Enterococcus hirae copper P-type ATPase, responsible for ... ...

Abstract	Downstream from the ptsHI operon of Lactobacillus sakei, the genes atkY and atkB, organized in an operon, were observed. The two putative proteins, AtkB and AtkY, show sequence similarity to the Enterococcus hirae copper P-type ATPase, responsible for copper efflux, and its negative regulator. Characterization of AtkB as a copper P-type ATPase could not be demonstrated since an atkB mutant did not show any phenotype. Thus, another strategy was followed in order to investigate the transcriptional regulation of the atkYB locus, leading to the development of new genetic tools for L. sakei. A plasmid was constructed, the use of which allowed gene replacement at the lacLM locus in L. sakei by two successive crossovers. A strain deleted of the lacLM operon encoding the beta-galactosidase of L. sakei was constructed by this method, and the Escherichia coli lacZ gene could then be used as a reporter gene to investigate the regulation of atkYB. Results show that the atkYB operon is induced by small concentrations of CuSO(4) (30 to 40 microM) but not when CuSO(4) is omitted or added at higher concentrations.
MeSH term(s)	Adenosine Triphosphatases/chemistry ; Adenosine Triphosphatases/genetics ; Amino Acid Sequence ; Bacterial Proteins ; Cloning, Molecular ; Crossing Over, Genetic ; Escherichia coli/genetics ; Gene Deletion ; Gene Expression Regulation, Bacterial ; Gene Expression Regulation, Enzymologic ; Genes, Reporter ; Lactobacillus/enzymology ; Lactobacillus/genetics ; Molecular Sequence Data ; Operon ; Polymerase Chain Reaction/methods ; Repressor Proteins/chemistry ; Repressor Proteins/genetics ; Sequence Alignment ; Sequence Homology, Amino Acid ; beta-Galactosidase/genetics
Chemical Substances	Bacterial Proteins ; Repressor Proteins ; beta-Galactosidase (EC 3.2.1.23) ; Adenosine Triphosphatases (EC 3.6.1.-) ; AtkB protein, Lactobacillus sakei (EC 3.6.1.-) ; AtkY protein, Lactobacillus sakei (EC 3.6.1.-)
Language	English
Publishing date	2000-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
DOI	10.1128/AEM.66.10.4272-4278.2000
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Article ; Online: Mucosal or systemic administration of rE2 glycoprotein antigen loaded PLGA microspheres.

Brandhonneur, N / Loizel, C / Chevanne, F / Wakeley, P / Jestin, A / Le Potier, M F / Le Corre, P

International journal of pharmaceutics

2009 Volume 373, Issue 1-2, Page(s) 16–23

Abstract: We have evaluated the ability of recombinant E2 antigen, as a surfactant free formulation of poly (D,L-lactide-co-glycolide) (PLGA) microspheres, to elicit a systemic immune response after administration by mucosal routes (oral and nasal) in comparison ... ...

Abstract	We have evaluated the ability of recombinant E2 antigen, as a surfactant free formulation of poly (D,L-lactide-co-glycolide) (PLGA) microspheres, to elicit a systemic immune response after administration by mucosal routes (oral and nasal) in comparison to intramuscular route. The sequence encoding a truncated E2 glycoprotein of the classical swine fever virus (CSFV) was expressed in insect cells following infection with recombinant baculovirus, as a His-tagged recombinant antigen. The recombinant E2 glycoprotein (rE2) antigen was co-encapsulated with rabbit serum albumin (RSA) as a protein stabilizer. rE2/RSA loaded PLGA microspheres, with a mean diameter of 4 microm were obtained by a water in oil in water solvent extraction method (w/o/w). Rabbits were immunized with 10 microg of rE2 formulated in PLGA microspheres administrated by three different routes (oral, nasal and intramuscular). After 60 days, each rabbit in all three groups was challenge with 5 microg of rE2 glycoprotein solution by intradermal administration. Blood samples were collected weekly for 90 days and specific rE2 antigen antibodies measured. This work showed that rE2 antigen loaded microspheres was able to initiate an immune response. The intradermal challenge after nasal and oral administration had a clear boost effect on the systemic immune response. Moreover, the response after nasal administration was more intense and less variable than oral route. In conclusion, these data demonstrate a high potential of rE2 loaded PLGA microspheres for their use as a mucosal subunit vaccine.
MeSH term(s)	Administration, Intranasal ; Administration, Oral ; Animals ; Antibody Formation/immunology ; Antigens, Viral/administration & dosage ; Antigens, Viral/immunology ; Biological Availability ; Classical swine fever virus/immunology ; Immunity, Mucosal/immunology ; Immunization, Secondary ; Immunoglobulins/blood ; Immunoglobulins/immunology ; Injections, Intradermal ; Injections, Intramuscular ; Lactic Acid/chemistry ; Microspheres ; Particle Size ; Polyglycolic Acid/chemistry ; Rabbits ; Recombinant Fusion Proteins/administration & dosage ; Recombinant Fusion Proteins/immunology ; Recombinant Fusion Proteins/pharmacokinetics ; Serum Albumin/chemistry ; Serum Albumin/pharmacokinetics ; Vaccination ; Vaccines, Subunit/administration & dosage ; Vaccines, Subunit/immunology ; Viral Envelope Proteins/administration & dosage ; Viral Envelope Proteins/immunology ; Viral Envelope Proteins/pharmacokinetics ; Viral Vaccines/administration & dosage ; Viral Vaccines/immunology
Chemical Substances	Antigens, Viral ; Immunoglobulins ; Recombinant Fusion Proteins ; Serum Albumin ; Vaccines, Subunit ; Viral Envelope Proteins ; Viral Vaccines ; glycoprotein E2, classical swine fever virus ; polylactic acid-polyglycolic acid copolymer ; Polyglycolic Acid (26009-03-0) ; Lactic Acid (33X04XA5AT)
Language	English
Publishing date	2009-05-21
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	428962-6
ISSN	1873-3476 ; 0378-5173
ISSN (online)	1873-3476
ISSN	0378-5173
DOI	10.1016/j.ijpharm.2009.01.020
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Article: Development of genetic tools for Lactobacillus sakei: disruption of the beta-galactosidase gene and use of lacZ as a reporter gene to study regulation of the putative copper ATPase, AtkB

Stentz, R / Loizel, C / Malleret, C / Zagorec, M

Applied and environmental microbiology. Oct 2000. v. 66 (10)

2000

Abstract	Downstream from the ptsHI operon of Lactobacillus sakei, the genes atkY and atkB, organized in an operon, were observed. The two putative proteins, AtkB and AtkY, show sequence similarity to the Enterococcus hirae copper P-type ATPase, responsible for copper efflux, and its negative regulator. Characterization of AtkB as a copper P-type ATPase could not be demonstrated since an atkB mutant did not show any phenotype. Thus, another strategy was followed in order to investigate the transcriptional regulation of the atkYB locus, leading to the development of new genetic tools for L. sakei. A plasmid was constructed, the use of which allowed gene replacement at the lacLM locus in L. sakei by two successive crossovers. A strain deleted of the lacLM operon encoding the beta-galactosidase of L. sakei was constructed by this method, and the Escherichia coli lacZ gene could then be used as a reporter gene to investigate the regulation of atkYB. Results show that the operon is induced by small concentrations of CuSO4 (30 to 40 micromolar) but not when CuSO4 is omitted or added at higher concentrations.
Keywords	Lactobacillus ; adenosinetriphosphatase ; genes ; nucleotide sequences ; mutants ; plasmid vectors ; gene expression ; copper sulfate ; reporter genes ; beta-galactosidase ; operon
Language	English
Dates of publication	2000-10
Size	p. 4272-4278.
Document type	Article
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
Database	NAL-Catalogue (AGRICOLA)

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Article: Reproduction of PMWS in immunostimulated SPF piglets transfected with infectious cloned genomic DNA of type 2 porcine circovirus.

Grasland, Béatrice / Loizel, Christophe / Blanchard, Philippe / Oger, Aurélie / Nignol, Anne-Cécile / Bigarré, Laurent / Morvan, Hervé / Cariolet, Roland / Jestin, André

Veterinary research

2005 Volume 36, Issue 5-6, Page(s) 685–697

Abstract: Postweaning multisystemic wasting syndrome (PMWS) is a recently emerged disease affecting pigs. Type 2 porcine circovirus (PCV2) has been associated with this syndrome although other factors are required in association with this virus for PMWS expression. ...

Abstract	Postweaning multisystemic wasting syndrome (PMWS) is a recently emerged disease affecting pigs. Type 2 porcine circovirus (PCV2) has been associated with this syndrome although other factors are required in association with this virus for PMWS expression. The aim of this study was to investigate whether general immunostimulation (injections of keyhole limpet hemocyanin emulsified in incomplete Freund adjuvant and of thioglycollate medium) could strengthen the severity of PMWS in six-week-old specific-pathogen-free (SPF) piglets transfected with pure tandem-cloned PCV2 DNA by the intramuscular route. Non-immunostimulated piglets transfected with the viral clone did not present clinical signs but only mild pathological microlesions characteristic of PMWS. These piglets seroconverted and high viral genome loads and infectious titers were detected in the lymphoid organs at the end of the trial. Mild-to-moderate forms of PMWS were generally observed in the immunostimulated transfected piglets, as well as one severe form for a piglet (8003) which died. These piglets with mild-to-moderate forms had higher DNA loads than the transfected-only animals. Thus, viral replication was enhanced by immunostimulation. This is the first time that clinical PMWS has been reported in an SPF immunostimulated piglet infected with a pure inoculum consisting of tandem-cloned PCV2 DNA. This result confirms that PCV2 is the agent of PMWS and that immunostimulation could enhance PMWS in SPF piglets transfected with a PCV2 DNA clone.
MeSH term(s)	Animals ; Antibodies, Viral ; Antigens, Viral ; Circoviridae Infections/immunology ; Circoviridae Infections/veterinary ; Circovirus/genetics ; Circovirus/pathogenicity ; DNA, Viral ; Immunization ; Specific Pathogen-Free Organisms ; Swine ; Swine Diseases/immunology ; Transfection/methods ; Viral Load ; Virus Replication ; Wasting Syndrome/immunology ; Wasting Syndrome/veterinary ; Wasting Syndrome/virology
Chemical Substances	Antibodies, Viral ; Antigens, Viral ; DNA, Viral
Keywords	covid19
Language	English
Publishing date	2005-09
Publishing country	England
Document type	Journal Article
ZDB-ID	1146298-x
ISSN	1297-9716 ; 0928-4249
ISSN (online)	1297-9716
ISSN	0928-4249
DOI	10.1051/vetres:2005024
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Article: Effect of granulocyte-macrophage colony-stimulating factor on post-weaning multisystemic wasting syndrome in porcine circovirus type-2-transfected piglets.

Loizel, Christophe / Blanchard, Philippe / Grasland, Béatrice / Dory, Daniel / Oger, Aurélie / Nignol, Anne-Cécile / Cariolet, Roland / Jestin, André

International journal of experimental pathology

2005 Volume 86, Issue 1, Page(s) 33–43

Abstract: Post-weaning multisystemic wasting syndrome (PMWS) is a complex disease syndrome in swine, affecting nursery and fattening pigs. Although ongoing evidence suggests that porcine circovirus type-2 (PCV2) is the causal agent of PMWS, the host immune system ... ...

Abstract	Post-weaning multisystemic wasting syndrome (PMWS) is a complex disease syndrome in swine, affecting nursery and fattening pigs. Although ongoing evidence suggests that porcine circovirus type-2 (PCV2) is the causal agent of PMWS, the host immune system appears to have a crucial role in the PMWS pathogenesis of PCV2-affected pigs. Owing to difficulties in producing a biologically pure form of PCV2 devoid of the other viral agents commonly present in swine tissues, we decided to use a tandem-cloned PCV2 DNA providing highly pure grade reagent in order to monitor the virulence of PCV2 alone or with an immunostimulating co-factor, granulocyte-macrophage colony-stimulating factor (GM-CSF). A single intramuscular injection of tandem-cloned PCV2 DNA into 5-week-old piglets produced plasmid to viral genome progeny and infectious particles as early as 8 days post-injection in all the organs tested (the lung, the tonsil and the inguinal, mesenteric, bronchial and upper-right axial lymph nodes). The initial plasmid load was not detected with the help of primers designed to specifically detect the acceptor plasmid, thus confirming the replication of the viral genome. Despite the presence of a high level of PCV2 genome copies in the lymphoid organs--the tonsil and the lung--and the presence of infectious particles, no detectable clinical manifestations or pathological lesions were observed in the transfected pigs over the period of observation, regardless of whether they had been co-injected with plasmid containing GM-CSF DNA or had received plasmid containing PCV2 DNA alone. GM-CSF encoding DNA injection had no significant effect on viral replication or on the production of viral particles and appearance of the disease.
MeSH term(s)	Animals ; Circoviridae Infections/immunology ; Circoviridae Infections/virology ; Circovirus/growth & development ; Circovirus/pathogenicity ; DNA, Viral/genetics ; Enzyme-Linked Immunosorbent Assay/methods ; Genetic Vectors ; Genome, Viral ; Granulocyte-Macrophage Colony-Stimulating Factor/genetics ; Granulocyte-Macrophage Colony-Stimulating Factor/physiology ; Lymph Nodes/virology ; Plasmids/genetics ; Polymerase Chain Reaction/methods ; Swine ; Swine Diseases/immunology ; Swine Diseases/virology ; Transfection ; Wasting Syndrome/immunology ; Wasting Syndrome/virology ; Weaning
Chemical Substances	DNA, Viral ; Granulocyte-Macrophage Colony-Stimulating Factor (83869-56-1)
Language	English
Publishing date	2005-02
Publishing country	England
Document type	Journal Article
ZDB-ID	1016006-1
ISSN	1365-2613 ; 0959-9673 ; 0958-4625 ; 0007-1021
ISSN (online)	1365-2613
ISSN	0959-9673 ; 0958-4625 ; 0007-1021
DOI	10.1111/j.0959-9673.2005.00409.x
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Article: Effective protection of pigs against lethal Pseudorabies virus infection after a single injection of low-dose Sindbis-derived plasmids encoding PrV gB, gC and gD glycoproteins.

Dory, Daniel / Torché, Anne-Marie / Béven, Véronique / Blanchard, Philippe / Loizel, Christophe / Cariolet, Roland / Jestin, André

Vaccine

2005 Volume 23, Issue 26, Page(s) 3483–3491

Abstract: This study compared protection of pigs against lethal Pseudorabies virus (PrV) infection induced by a single injection of various quantities of Sindbis virus-derived plasmids encoding PrV glycoproteins gB, gC and gD. Pigs were injected with 340, 68 or 13 ...

Abstract	This study compared protection of pigs against lethal Pseudorabies virus (PrV) infection induced by a single injection of various quantities of Sindbis virus-derived plasmids encoding PrV glycoproteins gB, gC and gD. Pigs were injected with 340, 68 or 13 microg of each plasmid. Few immune differences were observed after DNA injection and more importantly the pigs of the three groups were equally protected against virulent PrV infection. Single-shot injection of 13 microg of each PrV glycoprotein encoding Sindbis virus-derived plasmid is able to effectively protect pigs from PrV infection.
MeSH term(s)	Animals ; Genetic Vectors/genetics ; Herpesvirus 1, Suid/drug effects ; Herpesvirus 1, Suid/genetics ; Herpesvirus 1, Suid/immunology ; Pseudorabies/immunology ; Pseudorabies/prevention & control ; Pseudorabies Vaccines/administration & dosage ; Pseudorabies Vaccines/immunology ; Swine Diseases/immunology ; Swine Diseases/prevention & control ; Vaccines, DNA/administration & dosage ; Vaccines, DNA/immunology ; Viral Envelope Proteins/genetics ; Viral Envelope Proteins/immunology
Chemical Substances	Pseudorabies Vaccines ; Vaccines, DNA ; Viral Envelope Proteins
Language	English
Publishing date	2005-05-16
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2004.10.050
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Article: Circovirus et pathologies associées chez les animaux.

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Article: Development of genetic tools for Lactobacillus sakei: disruption of the beta-galactosidase gene and use of lacZ as a reporter gene To study regulation of the putative copper ATPase, AtkB.

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Article ; Online: Mucosal or systemic administration of rE2 glycoprotein antigen loaded PLGA microspheres.

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Article: Development of genetic tools for Lactobacillus sakei: disruption of the beta-galactosidase gene and use of lacZ as a reporter gene to study regulation of the putative copper ATPase, AtkB

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Article: Reproduction of PMWS in immunostimulated SPF piglets transfected with infectious cloned genomic DNA of type 2 porcine circovirus.

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Article: Effect of granulocyte-macrophage colony-stimulating factor on post-weaning multisystemic wasting syndrome in porcine circovirus type-2-transfected piglets.

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Article: Effective protection of pigs against lethal Pseudorabies virus infection after a single injection of low-dose Sindbis-derived plasmids encoding PrV gB, gC and gD glycoproteins.

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