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  1. Article ; Online: Selection and characterization of Bacillus thuringiensis strains from northwestern Himalayas toxic against Helicoverpa armigera.

    Lone, Showkat A / Malik, Abdul / Padaria, Jasdeep C

    MicrobiologyOpen

    2017  Volume 6, Issue 6

    Abstract: In this study, we present the selection and the characterization of Bacillus thuringiensis (Bt) strains with respect to their cry/cyt gene content and toxicity evaluation toward one of the most important polyphagous lepidopteran pest, Helicoverpa ... ...

    Abstract In this study, we present the selection and the characterization of Bacillus thuringiensis (Bt) strains with respect to their cry/cyt gene content and toxicity evaluation toward one of the most important polyphagous lepidopteran pest, Helicoverpa armigera. Fifty-six Bt isolates were obtained from 10 different regions of northwestern Himalayas, recording a total B. thuringiensis index of 0.62. Scanning electron microscopy revealed presence of bipyramidal, spherical, flat and irregular crystal shapes; SDS-PAGE analysis of spore-crystal mixtures showed the prominence of 130, 70, and 100 kDa protein bands in majority of the isolates; PCR analysis with primers for eight cry and cyt gene families and 13 cry gene subfamilies resulted in isolates showing different combinations of insecticidal genes. Strains containing cry1 were the most abundant (57.1%) followed by cyt2 (46.42%), cry11 (37.5%), cry2 (28.57%), cry4 (21.42%), cyt1 (19.64%), cry3 (8.9%), and cry7, 8 (7.14%). A total of 30.35% of the strains did not amplify with any of the primers used in this study. Median lethal concentration 50 (LC
    MeSH term(s) Animals ; Bacillus thuringiensis/chemistry ; Bacillus thuringiensis/classification ; Bacillus thuringiensis/genetics ; Bacillus thuringiensis/isolation & purification ; Bacillus thuringiensis Toxins ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Bacterial Proteins/toxicity ; DNA Primers/genetics ; Electrophoresis, Polyacrylamide Gel ; Endotoxins/chemistry ; Endotoxins/genetics ; Endotoxins/metabolism ; Endotoxins/toxicity ; Hemolysin Proteins/chemistry ; Hemolysin Proteins/genetics ; Hemolysin Proteins/metabolism ; Hemolysin Proteins/toxicity ; Moths/drug effects ; Moths/physiology ; Pest Control, Biological ; Polymerase Chain Reaction ; Soil Microbiology
    Chemical Substances Bacillus thuringiensis Toxins ; Bacterial Proteins ; DNA Primers ; Endotoxins ; Hemolysin Proteins ; insecticidal crystal protein, Bacillus Thuringiensis
    Language English
    Publishing date 2017-10-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 2661368-2
    ISSN 2045-8827 ; 2045-8827
    ISSN (online) 2045-8827
    ISSN 2045-8827
    DOI 10.1002/mbo3.484
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Chromium Toxicity in Plants: Signaling, Mitigation, and Future Perspectives.

    Ali, Sajad / Mir, Rakeeb A / Tyagi, Anshika / Manzar, Nazia / Kashyap, Abhijeet Shankar / Mushtaq, Muntazir / Raina, Aamir / Park, Suvin / Sharma, Sandhya / Mir, Zahoor A / Lone, Showkat A / Bhat, Ajaz A / Baba, Uqab / Mahmoudi, Henda / Bae, Hanhong

    Plants (Basel, Switzerland)

    2023  Volume 12, Issue 7

    Abstract: Plants are very often confronted by different heavy metal (HM) stressors that adversely impair their growth and productivity. Among HMs, chromium (Cr) is one of the most prevalent toxic trace metals found in agricultural soils because of anthropogenic ... ...

    Abstract Plants are very often confronted by different heavy metal (HM) stressors that adversely impair their growth and productivity. Among HMs, chromium (Cr) is one of the most prevalent toxic trace metals found in agricultural soils because of anthropogenic activities, lack of efficient treatment, and unregulated disposal. It has a huge detrimental impact on the physiological, biochemical, and molecular traits of crops, in addition to being carcinogenic to humans. In soil, Cr exists in different forms, including Cr (III) "trivalent" and Cr (VI) "hexavalent", but the most pervasive and severely hazardous form to the biota is Cr (VI). Despite extensive research on the effects of Cr stress, the exact molecular mechanisms of Cr sensing, uptake, translocation, phytotoxicity, transcript processing, translation, post-translational protein modifications, as well as plant defensive responses are still largely unknown. Even though plants lack a Cr transporter system, it is efficiently accumulated and transported by other essential ion transporters, hence posing a serious challenge to the development of Cr-tolerant cultivars. In this review, we discuss Cr toxicity in plants, signaling perception, and transduction. Further, we highlight various mitigation processes for Cr toxicity in plants, such as microbial, chemical, and nano-based priming. We also discuss the biotechnological advancements in mitigating Cr toxicity in plants using plant and microbiome engineering approaches. Additionally, we also highlight the role of molecular breeding in mitigating Cr toxicity in sustainable agriculture. Finally, some conclusions are drawn along with potential directions for future research in order to better comprehend Cr signaling pathways and its mitigation in sustainable agriculture.
    Language English
    Publishing date 2023-03-29
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants12071502
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Validation and development of RP-HPLC method for quantification of glibenclamide in rat plasma and its application to pharmacokinetic studies in wistar rats.

    Mir, Khalid Bashir / Abrol, Vidushi / Wani, Taha U / Jan, Ishrat / Singh, Nasseb / Khan, Nisar A / Dar, Alamgir A / Sabri Sultan, Rania Mohammad / Lone, Showkat A / Iesa, Mohamed A M / Alhag, Sadeq K / Al-Shuraym, Laila A / Helm, Nawal / Al-Farga, Ammar

    Heliyon

    2023  Volume 9, Issue 11, Page(s) e20876

    Abstract: Herein, we report a novel, simple, specific, accurate and cost-friendly validated reverse phase-high performance liquid chromatographic (RP-HPLC) method for the quantification of second generation sulphonylurea based antidiabetic drug, glibenclamide (GLB) ...

    Abstract Herein, we report a novel, simple, specific, accurate and cost-friendly validated reverse phase-high performance liquid chromatographic (RP-HPLC) method for the quantification of second generation sulphonylurea based antidiabetic drug, glibenclamide (GLB) in rat plasma and its application to calculate pharmacokinetic parameters in wistar rats. The internal standard used was flufenamic acid. The chromatographic separation was conducted on C
    Language English
    Publishing date 2023-10-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2023.e20876
    Database MEDical Literature Analysis and Retrieval System OnLINE

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