LIVIVO - Search results -

Search results

Result 1 - 10 of total 32

Search options

Book ; Online: Advances in Postharvest Pathology of Fruits and Vegetables

Li, Boqiang / Long, Chao-an / Zhang, Hongyin / Tao, Nengguo

2020

Keywords	Science: general issues ; Medical microbiology & virology ; Microbiology (non-medical) ; Fruit and Vegetables ; Postharvest Pathogens ; Mycotoxin ; Interactions ; Fungus
Size	1 electronic resource (189 pages)
Publisher	Frontiers Media SA
Document type	Book ; Online
Note	English ; Open Access
HBZ-ID	HT021229936
ISBN	9782889633227 ; 2889633225
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Genome identification and biocontrol potential against citrus blue mold of Bacillus velezensis w176

Tian, Zhonghuan / Du, Yujie / Lu, Yongqing / Ji, Sirong / Long, Chao-an

Food Control. 2023 Dec., v. 154 p.109977-

2023

Abstract: Citrus fruit is susceptible to Penicillium mold infection. Biocontrol with utilization of antagonistic microorganisms against postharvest diseases is regarded as an effective and eco-friendly approach in citrus fruit storage. Here, the complete genome of ...

Abstract	Citrus fruit is susceptible to Penicillium mold infection. Biocontrol with utilization of antagonistic microorganisms against postharvest diseases is regarded as an effective and eco-friendly approach in citrus fruit storage. Here, the complete genome of biocontrol strain w176 was obtained with a full length of 3,929,790 bp, and it consists of 12 potential gene clusters which might be responsible for antagonistic metabolites biosynthesis, strain w176 was identified as Bacillus velezensis by genome alignments eventually. Meanwhile, strong restrictions of spore germination rate (SGR) and hyphae elongation were detected when strain w176 was co-incubated with P. italicum, the inhibitory of SGR at 6 h, 9 h and 12 h was 10.00%, 49.59% and 23.53%, respectively. And the inhibitory of hyphae elongation was 35.56% at 12 h. It was also found that strain w176 exhibited notable growth space competition in the co-incubation. Moreover, significantly enhanced activities of catalase and chitinase in citrus fruit were presented 2 d and 3 d post-inoculation with strain w176. Further, citrus fruit treated by strain w176 PDB culture (PBC) or its culture filtrate (CFS) displayed significantly reduced virulence of P. italicum contamination, the decreased disease symptoms of CFS and PBC were 30.32% and 85.22%, individually. The inhibitory disease rate of PBC was 25%. This study reveals that strain w176 has high potential to be applied as bio-pesticide in citrus preservation. The data attained in this research might be helpful for citrus blue mold control, and could provide basis for the bio-preservation study in postharvest fruit.
Keywords	Bacillus velezensis ; Citrus ; Penicillium ; biological control ; biopesticides ; biosynthesis ; catalase ; chitinase ; citrus fruits ; culture filtrates ; food safety ; fruits ; genes ; hyphae ; metabolites ; molds (fungi) ; spore germination ; virulence ; Biocontrol ; Penicillium italicum ; Postharvest
Language	English
Dates of publication	2023-12
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	1027805-9
ISSN	0956-7135
ISSN	0956-7135
DOI	10.1016/j.foodcont.2023.109977
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 5255: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: PdGpaA controls the growth and virulence of Penicillium digitatum by regulating cell wall reorganization, energy metabolism, and CDWEs production

Du, Yujie / Zhu, Jinfan / Zhang, Wan / Chen, Xiao / Yang, Fan / Tian, Zhonghuan / Long, Chao-an

Postharvest Biology and Technology. 2023 Oct., v. 204 p.112441-

2023

Abstract: Penicillium digitatum could infect citrus and cause devastating green mold disease. One of the essential parts of the G protein is the Gα subunit, which is essential to signal transduction in filamentous fungi but has not been reported in P. digitatum. ... ...

Abstract	Penicillium digitatum could infect citrus and cause devastating green mold disease. One of the essential parts of the G protein is the Gα subunit, which is essential to signal transduction in filamentous fungi but has not been reported in P. digitatum. In this study, PdGpaA was identified as a homolog of Gα in P. digitatum, and deletion of the PdGpaA gene significantly accelerated early-stage spore germination and inhibited late-stage hyphal growth and conidiophore formation. The ΔPdGpaA strain showed faster growth in sodium dodecyl sulfate (SDS) and calcofluor white (CFW) but more sensitivity to Congo red (CR). We predicted that cell wall integrity had been compromised due to cell wall rearrangement. Moreover, the increased quantity of septa and the reorganization of cell walls were confirmed by CFW staining and quantitative real-time PCR analysis. Deletion of the PdGpaA gene increased intracellular reactive oxygen species (ROS)and mitochondrial deterioration, which obstruct the TCA cycle and hinder energy metabolism. A pathogenicity test revealed that the lesion diameter was decreased by 90.62 % in the ΔPdGpaA strain, probably as the result of a decline in the activity of pectinase and cellulase. In addition, the ΔPdGpaA strain was more sensitive to prochloraz, KCl and H₂O₂. In conclusion, this study provides multiple functions for the PdGpaA gene, which might have broad application space and prospects in the control of citrus green mold.
Keywords	Biological Sciences ; Citrus ; Penicillium digitatum ; cell walls ; endo-1,4-beta-glucanase ; energy metabolism ; genes ; hyphae ; mitochondria ; polygalacturonase ; prochloraz ; quantitative polymerase chain reaction ; reactive oxygen species ; signal transduction ; sodium dodecyl sulfate ; spore germination ; technology ; tricarboxylic acid cycle ; virulence ; Gα protein ; Cell wall integrity ; ROS ; Pathogenicity ; Fungicide sensitivity ; CDWEs
Language	English
Dates of publication	2023-10
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	1082798-5
ISSN	1873-2356 ; 0925-5214
ISSN (online)	1873-2356
ISSN	0925-5214
DOI	10.1016/j.postharvbio.2023.112441
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 5311: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article: Functional identification of the DHN melanin synthesis gene cluster and its role in UV-C tolerance in citrus postharvest pathogenic fungus Penicillium digitatum

Yang, Fan / Cheng, Lingtong / Du, Yuling / Xia, Lungang / Long, Chao-an

British Mycological Society Fungal biology. 2022 July 11,

2022

Abstract: The filamentous fungus Penicillium digitatum brings out great losses in citrus fruits by causing citrus green mold disease during the postharvest period. Previously, we obtained a T-DNA insertion mutant N2130 of P. digitatum, which produced albino ... ...

Abstract	The filamentous fungus Penicillium digitatum brings out great losses in citrus fruits by causing citrus green mold disease during the postharvest period. Previously, we obtained a T-DNA insertion mutant N2130 of P. digitatum, which produced albino conidia. To understand the role of green-grey conidial pigment in P. digitatum, we identified the insertion site and deeply explored the 1,8-dihydroxynaphtsalene (DHN)-melanin synthesis gene cluster in this phytopathogen. In this study, we deleted five genes in P. digitatum, PdPksP, PdAbr1, PdArp1, PdArp2, and PdAyg1, and the experiments were further performed on phenotype analyses, including pigmentation, UV-C tolerance, virulence, growth rate, conidiation, stress (osmotic-, oxidative-, cell wall disturbing-, and high temperature-) tolerance, fungicide resistance, and conidial hydrophobicity. The results showed that the five deletion mutants (ΔPdPksP, ΔPdAbr1, ΔPdArp1, ΔPdArp2 and ΔPdAyg1) produced albino, brownish, brown, reddish-brown, and Yellowish green conidia, respectively. In addition, the survival colony forming units (CFUs) of the deletion mutants, under the treatment of UV-C radiation (261.4 mJ/cm²), were 0.3- to 0.6-fold of those surviving in wild-type strain N1. Moreover, after 522.8 mJ/cm²-UV-C-irradiation on conidia, the deletion mutants showed a larger decrease in pathogenicity on Valencia Orange fruits compared with strain N1. However, there were no significant differences among other phenotypes tested in this study. Collectively, our research reported the DHN-melanin synthesis pathway in P. digitatum for the first time, and revealed that DHN-melanin is important for P. digitatum to tolerate UV-C irradiation.
Keywords	Citrus sinensis ; Penicillium digitatum ; albino ; cell walls ; conidia ; conidiation ; fungi ; fungicide resistance ; hydrophobicity ; irradiation ; melanin ; multigene family ; mutants ; mycology ; pigmentation ; plant pathogens ; virulence ; virulent strains
Language	English
Dates of publication	2022-0711
Publishing place	Elsevier Ltd
Document type	Article
Note	Pre-press version
ZDB-ID	2532164-X
ISSN	1878-6162 ; 1878-6146
ISSN (online)	1878-6162
ISSN	1878-6146
DOI	10.1016/j.funbio.2022.07.002
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: Curative activity of KCl treatments to control citrus sour rot

Zhao, Juan / Liu, Qianyi / Wang, Yuqing / Lu, Xinjun / Pan, Hui / Long, Chao-an

Lebensmittel-Wissenschaft + Technologie. 2022 Aug. 04,

2022

Abstract: The potassium chloride (KCl), potassium nitrate (KNO₃) and potassium sulfate (K₂SO₄) could inhibit G. citri-aurantii development in vitro, and control the efficient soaking of 0.1 and 0.5 M KCl reached 14.1% and 46.3% in vivo. In addition, 0.5 M KCl ... ...

Abstract	The potassium chloride (KCl), potassium nitrate (KNO₃) and potassium sulfate (K₂SO₄) could inhibit G. citri-aurantii development in vitro, and control the efficient soaking of 0.1 and 0.5 M KCl reached 14.1% and 46.3% in vivo. In addition, 0.5 M KCl treatment made no difference on fruit quality of weight loss, soluble solid and titratable acid neither in the incidence of sour rot, which decreased to 2.35% during 90 days storage period. Furthermore, the citrus peel enzyme activity of CAT, PPO and Cellulase significant increased, G. citri-aurantii spores were gathered into long chain shape preventing spore germination and lipid droplets (LDs) accumulated in large quantities after KCl treatment by TEM. In addition, transcriptome sequencing analysis was confirmed and the six genes (Gci002087, Gci003354, Gci000394, Gci003505, Gci004433 and Gci000013) involved in target of rapamycin (TOR) pathway. The results of this study provide a new direction for sour rot prevention.
Keywords	Citrus ; citrus peels ; endo-1,4-beta-glucanase ; enzyme activity ; fruit quality ; lipids ; potassium chloride ; potassium nitrate ; potassium sulfate ; rapamycin ; spore germination ; storage time ; titratable acidity ; total soluble solids ; transcriptome ; weight loss
Language	English
Dates of publication	2022-0804
Publishing place	Elsevier Ltd
Document type	Article
Note	Pre-press version
ZDB-ID	241369-3
ISSN	0460-1173 ; 0023-6438
ISSN	0460-1173 ; 0023-6438
DOI	10.1016/j.lwt.2022.113853
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 2634: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Natamycin as a safe food additive to control postharvest green mould and sour rot in citrus

Du, Yujie / Li, Yajuan / Tian, Zhonghuan / Cheng, Yunjiang / Long, Chao‐an

Journal of Applied Microbiology. 2022 Dec., v. 133, no. 6 p.3438-3450

2022

Abstract: AIMS: The purpose of this study was to explore the potential inhibitory mechanism and assess the feasibility of natamycin as an antifungal agent in the utilization of citrus storage. METHODS AND RESULTS: In this study, the mycelial growth, spore ... ...

Abstract	AIMS: The purpose of this study was to explore the potential inhibitory mechanism and assess the feasibility of natamycin as an antifungal agent in the utilization of citrus storage. METHODS AND RESULTS: In this study, the mycelial growth, spore germination as well as germ tube elongations of Geotrichum citri‐aurantii and Penicillium digitatum were significantly inhibited by natamycin treatment. The relative conductivities of G. citri‐aurantii and P. digitatum mycelia were increased as time went by and the damages of plasma membranes were up to 17.43% and 28.61%. The mitochondria abnormalities and vacuolation were also observed in the TEM. Moreover, the sour rot and green mould decay incidences were reduced to 18.33% and 10% post incubation with G. citri‐aurantii and P. digitatum under 300 mg L⁻¹ natamycin application, respectively. For the citrus storage experiment, there was no significant difference in edible rate, juice yield, total soluble solid (TSS) content, titratable acid (TA) and decay incidences of the ‘Newhall’ navel orange fruit treated with 300 mg L⁻¹ natamycin stored for 90 d. CONCLUSIONS: Natamycin could decrease the expansions of green mould and sour rot and maintain quality and improve storability on citrus fruit. SIGNIFICANCE AND IMPACT OF THE STUDY: This work explores the potential inhibition mechanism of natamycin G. citri‐aurantii and P. digitatum and assesses the feasibility of natamycin as an antifungal agent in the utilization of citrus storage.
Keywords	Citrus sinensis ; Geotrichum ; Penicillium digitatum ; citrus fruits ; food additives ; fruits ; germ tube ; juices ; microbiology ; mitochondria ; mycelium ; natamycin ; spore germination ; storage quality ; titratable acidity ; total soluble solids
Language	English
Dates of publication	2022-12
Size	p. 3438-3450.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	1358023-1
ISSN	1365-2672 ; 1364-5072
ISSN (online)	1365-2672
ISSN	1364-5072
DOI	10.1111/jam.15769
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 259: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Natamycin as a safe food additive to control postharvest green mould and sour rot in citrus.

Du, Yujie / Li, Yajuan / Tian, Zhonghuan / Cheng, Yunjiang / Long, Chao-An

Journal of applied microbiology

2022 Volume 133, Issue 6, Page(s) 3438–3450

Abstract: Aims: The purpose of this study was to explore the potential inhibitory mechanism and assess the feasibility of natamycin as an antifungal agent in the utilization of citrus storage.: Methods and results: In this study, the mycelial growth, spore ... ...

Abstract	Aims: The purpose of this study was to explore the potential inhibitory mechanism and assess the feasibility of natamycin as an antifungal agent in the utilization of citrus storage. Methods and results: In this study, the mycelial growth, spore germination as well as germ tube elongations of Geotrichum citri-aurantii and Penicillium digitatum were significantly inhibited by natamycin treatment. The relative conductivities of G. citri-aurantii and P. digitatum mycelia were increased as time went by and the damages of plasma membranes were up to 17.43% and 28.61%. The mitochondria abnormalities and vacuolation were also observed in the TEM. Moreover, the sour rot and green mould decay incidences were reduced to 18.33% and 10% post incubation with G. citri-aurantii and P. digitatum under 300 mg L Conclusions: Natamycin could decrease the expansions of green mould and sour rot and maintain quality and improve storability on citrus fruit. Significance and impact of the study: This work explores the potential inhibition mechanism of natamycin G. citri-aurantii and P. digitatum and assesses the feasibility of natamycin as an antifungal agent in the utilization of citrus storage.
MeSH term(s)	Citrus/microbiology ; Natamycin/pharmacology ; Antifungal Agents/pharmacology ; Food Additives ; Plant Diseases/prevention & control ; Plant Diseases/microbiology ; Fungi ; Penicillium ; Fruit/microbiology
Chemical Substances	Natamycin (8O0C852CPO) ; Antifungal Agents ; Food Additives
Language	English
Publishing date	2022-08-31
Publishing country	England
Document type	Journal Article
ZDB-ID	1358023-1
ISSN	1365-2672 ; 1364-5072
ISSN (online)	1365-2672
ISSN	1364-5072
DOI	10.1111/jam.15769
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 259: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Functional identification of the DHN melanin synthesis gene cluster and its role in UV-C tolerance in citrus postharvest pathogenic fungus Penicillium digitatum.

Yang, Fan / Cheng, Lingtong / Du, Yuling / Xia, Lungang / Long, Chao-An

Fungal biology

2022 Volume 126, Issue 9, Page(s) 566–575

Abstract	The filamentous fungus Penicillium digitatum brings out great losses in citrus fruits by causing citrus green mold disease during the postharvest period. Previously, we obtained a T-DNA insertion mutant N2130 of P. digitatum, which produced albino conidia. To understand the role of green-grey conidial pigment in P. digitatum, we identified the insertion site and deeply explored the 1,8-dihydroxynaphtsalene (DHN)-melanin synthesis gene cluster in this phytopathogen. In this study, we deleted five genes in P. digitatum, PdPksP, PdAbr1, PdArp1, PdArp2, and PdAyg1, and the experiments were further performed on phenotype analyses, including pigmentation, UV-C tolerance, virulence, growth rate, conidiation, stress (osmotic-, oxidative-, cell wall disturbing-, and high temperature-) tolerance, fungicide resistance, and conidial hydrophobicity. The results showed that the five deletion mutants (ΔPdPksP, ΔPdAbr1, ΔPdArp1, ΔPdArp2 and ΔPdAyg1) produced albino, brownish, brown, reddish-brown, and Yellowish green conidia, respectively. In addition, the survival colony forming units (CFUs) of the deletion mutants, under the treatment of UV-C radiation (261.4 mJ/cm<sup>2</sup>), were 0.3- to 0.6-fold of those surviving in wild-type strain N1. Moreover, after 522.8 mJ/cm<sup>2</sup>-UV-C-irradiation on conidia, the deletion mutants showed a larger decrease in pathogenicity on Valencia Orange fruits compared with strain N1. However, there were no significant differences among other phenotypes tested in this study. Collectively, our research reported the DHN-melanin synthesis pathway in P. digitatum for the first time, and revealed that DHN-melanin is important for P. digitatum to tolerate UV-C irradiation.
MeSH term(s)	Citrus/microbiology ; Fungal Proteins/genetics ; Gene Expression Regulation, Fungal ; Melanins/metabolism ; Multigene Family ; Penicillium ; Spores, Fungal
Chemical Substances	Fungal Proteins ; Melanins
Language	English
Publishing date	2022-07-16
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2532164-X
ISSN	1878-6162 ; 1878-6146
ISSN (online)	1878-6162
ISSN	1878-6146
DOI	10.1016/j.funbio.2022.07.002
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: GAR-transferase contributes to purine synthesis and mitochondrion function to maintain fungal development and full virulence of Penicillium digitatum.

Yang, Fan / Lu, Yongqing / Du, Yulin / Liu, Shuqi / Zhong, Xiuying / Du, Yujie / Tian, Zhonghuan / Long, Chao-An

International journal of food microbiology

2023 Volume 394, Page(s) 110177

Abstract: Penicillium digitatum is one of the most critical phytopathogens during the citrus postharvest period. However, the molecular mechanism of pathogenesis remains to be further explored. Purine is a multiple functional substance in organisms. To verify the ... ...

Abstract	Penicillium digitatum is one of the most critical phytopathogens during the citrus postharvest period. However, the molecular mechanism of pathogenesis remains to be further explored. Purine is a multiple functional substance in organisms. To verify the role of the de novo purine biosynthesis (DNPB) pathway in P. digitatum, we investigated the third gene Pdgart, glycinamide ribonucleotide (GAR)-transferase, of this pathway in this study. The deletion mutant ΔPdgart was generated in the principle of homologous recombination via Agrobacterium tumefaciens-mediated transformation (ATMT). The phenotypic assay indicated that the ΔPdgart mutant displayed severe defects in hyphae growth, conidiation and germination, which can be rescued by the addition of exogenous ATP and AMP. Compared with wild-type strain N1, the ATP level of strain ΔPdgart was detected to be sharply declined during conidial germination, and this was resulted from the damage to purine synthesis and aerobic respiration. The pathogenicity assay suggested that mutant ΔPdgart infected citrus fruit but attenuated disease, which was owing to its reduced production of organic acids and activities of cell wall degradation enzymes. Additionally, the ΔPdgart mutant showed altered sensitivity to stress agents and fungicides. Taken together, the present study provides insights into the essential functions of Pdgart, and paves the way for further study and novel fungicide development.
MeSH term(s)	Virulence/genetics ; Fungal Proteins/genetics ; Transferases/metabolism ; Citrus/microbiology ; Penicillium/metabolism ; Fungicides, Industrial/pharmacology ; Mitochondria/metabolism ; Purines/metabolism ; Adenosine Triphosphate/metabolism ; Plant Diseases/microbiology
Chemical Substances	glycinamide ribonucleotide ; Fungal Proteins ; Transferases (EC 2.-) ; Fungicides, Industrial ; Purines ; Adenosine Triphosphate (8L70Q75FXE)
Language	English
Publishing date	2023-03-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	87122-9
ISSN	1879-3460 ; 0168-1605
ISSN (online)	1879-3460
ISSN	0168-1605
DOI	10.1016/j.ijfoodmicro.2023.110177
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4668: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Antifungal activity of natamycin against kiwifruit soft rot caused by Botryosphaeria dothidea and potential mechanisms

Pan, Hui / Zhong, Caihong / Xia, Lungang / Li, Wenyi / Wang, Zupeng / Deng, Lei / Li, Li / Long, Chao-an

Scientia horticulturae. 2022 Nov. 17, v. 305

2022

Abstract: Soft rot caused by Botryosphaeria dothidea leads to massive economic losses in the kiwifruit industry. Natamycin is a natural antimicrobial preservative that effectively prevents postharvest decay and preserves fruit quality. However, the ability to ... ...

Abstract	Soft rot caused by Botryosphaeria dothidea leads to massive economic losses in the kiwifruit industry. Natamycin is a natural antimicrobial preservative that effectively prevents postharvest decay and preserves fruit quality. However, the ability to control kiwifruit soft rot and potential antifungal mechanisms have not been explored. In this study, the inhibitory impact of natamycin against soft rot caused by B. dothidea, as well as its putative action mechanisms were investigated. The results indicated that 2 mg L⁻¹ natamycin effectively inhibited mycelial growth and spore germination of B. dothidea in vitro. Natamycin caused B. dothidea hypha to shrink and deform, along with vacuolation and plasmolysis in hypha cells, as observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Likewise, natamycin stimulated the accumulation of reactive oxygen species (ROS) in the hypha. The inhibition effect of natamycin on B. dothidea inoculated in kiwifruit was dose-dependent, with 500 mg L⁻¹ natamycin significantly decreasing the incidence of soft rot to 35%. Furthermore, natamycin induced disease resistance in kiwifruit tissue by activating the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), increasing the antioxidant compound total phenol, maintaining a low level of malondialdehyde (MDA), and repressing the activity of four cell wall degrading enzymes, as well as gene expression of β-galactosidase (β-Gal) and polygalacturonase (PG). Further field research revealed that pre-harvest treatment combined with the postharvest natamycin application significantly reduced the natural decay incidence of kiwifruit while having no negative effect on soft-ripe quality. In conclusion, our findings proved that natamycin has an antifungal effect on B. dothidea and may be applied as a safe preservative to reduce soft rot.
Keywords	Botryosphaeria dothidea ; antifungal properties ; catalase ; cell walls ; disease resistance ; dose response ; fruit quality ; gene expression ; industry ; kiwifruit ; malondialdehyde ; mycelium ; natamycin ; phenols ; plasmolysis ; polygalacturonase ; postharvest diseases ; preharvest treatment ; reactive oxygen species ; spore germination ; superoxide dismutase ; transmission electron microscopy
Language	English
Dates of publication	2022-1117
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	185557-8
ISSN	0304-4238
ISSN	0304-4238
DOI	10.1016/j.scienta.2022.111344
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 2810: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED