LIVIVO - Search results -

Search results

Result 1 - 10 of total 22

Search options

Article: Expression Profiles of a Tung Tree Phosphate Transporter cDNA and Structural Characteristics of the Encoded Protein

Zhou, Junqin / Yuan, Jun / Long, Hongxu / Tan, Xiaofeng

Cytology and genetics. 2019 Mar., v. 53, no. 2

2019

Abstract: Plant phosphate transporters mediate phosphate acquisition, translocation, and recycling. A Vernicia fordii cDNA encoding a phosphate transporter was cloned using a reverse transcription polymerase chain reaction (PCR) and rapid amplification of cDNA ... ...

Abstract	Plant phosphate transporters mediate phosphate acquisition, translocation, and recycling. A Vernicia fordii cDNA encoding a phosphate transporter was cloned using a reverse transcription polymerase chain reaction (PCR) and rapid amplification of cDNA ends. The resulting cDNA named Vernicia fordii Phosphate Transporter-1;1 (VfPht1;1) encodes a predicted protein consisting of 533 amino acids. This protein, which was predicted to contain 12 transmembrane domains, is most similar (89.9%) to a Ricinus communis predicted protein. The quantitative real-time PCR data revealed that VfPht1;1 is most highly expressed in mature leaf petioles. It is also highly expressed in seeds collected in June, when developing fruits require a large amount of phosphates. Our observations suggest that VfPht1;1 may have diverse functions related to phosphate uptake and translocation.
Keywords	Ricinus communis ; Vernicia fordii ; phosphates ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction
Language	English
Dates of publication	2019-03
Size	p. 162-168.
Publishing place	Pleiades Publishing
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	390717-x
ISSN	1934-9440 ; 0095-4527
ISSN (online)	1934-9440
ISSN	0095-4527
DOI	10.3103/S0095452719020105
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 2982: Show issues

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: Hidden in plain sight: Systematic investigation of Leucine-rich repeat containing genes unveil the their regulatory network in response to Fusarium wilt in tung tree

Cao, Yunpeng / Liu, Meilan / Long, Hongxu / Zhao, Qiuyuan / Jiang, Lan / Zhang, Lin

International journal of biological macromolecules. 2020 Nov. 15, v. 163

2020

Abstract: Leucine-rich repeat-containing genes (LRRs) have been reported to play important roles in responses to diseases. However, we poorly understood the response of LRRs to Fusarium wilt infection in tung tree (Vernicia fordii), which is an important economic ... ...

Abstract	Leucine-rich repeat-containing genes (LRRs) have been reported to play important roles in responses to diseases. However, we poorly understood the response of LRRs to Fusarium wilt infection in tung tree (Vernicia fordii), which is an important economic tree. Here, 437 LRR-containing proteins containing nine types of LRR domains were identified in V. fordii genome. Phylogenetic analysis suggested that nine types of LRR domains could not be divided into separate classes, implying that these LRR domains had a common origin. Totally, 27 LRRs were related to possible resistance to Fusarium wilt after 2, 8, and 13 days post-inoculation. We further found that Vf06G1605 was up-regulate under Fusarium wilt infection after these three time points, Vf10G1602 and Vf02G1413 were up-regulated at 8, and 13 dpi, while Vf07G2320 was down-regulated at these three time points. The WGCNA and promoter elements suggested that WRKY possibly regulate the responses of LRRs to Fusarium wilt infection. This study highlighted the phylogeny and function of LRRs in V. fordii and provided a systematic analysis of these genes in the V. fordii genome. Our results presented here might clearly illustrate physiological mechanisms of resistance to Fusarium wilt infection and the target of marker-assisted breeding in V. fordii.
Keywords	Fusarium wilt ; Vernicia fordii ; phylogeny ; trees
Language	English
Dates of publication	2020-1115
Size	p. 1759-1767.
Publishing place	Elsevier B.V.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2020.09.106
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.B 2374: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Discovery of New Triterpenoids Extracted from Camellia oleifera Seed Cake and the Molecular Mechanism Underlying Their Antitumor Activity

Wu, Zelong / Tan, Xiaofeng / Zhou, Junqin / Yuan, Jun / Yang, Guliang / Li, Ze / Long, Hongxu / Yi, Yuhang / Lv, Chenghao / Zeng, Chaoxi / Qin, Si

Antioxidants. 2022 Dec. 21, v. 12, no. 1

2022

Abstract: Theasaponin derivatives, which are reported to exert antitumor activity, have been widely reported to exist in edible plants, including in the seed cake of Camellia oleifera (C.), which is extensively grown in south of China. The purpose of this study ... ...

Abstract	Theasaponin derivatives, which are reported to exert antitumor activity, have been widely reported to exist in edible plants, including in the seed cake of Camellia oleifera (C.), which is extensively grown in south of China. The purpose of this study was to isolate new theasaponin derivatives from C. seed cake and explore their potential antitumor activity and their underlying molecular mechanism. In the present study, we first isolated and identified four theasaponin derivatives (compounds 1, 2, 3, and 4) from the total aglycone extract of the seed cake of Camellia oleifera by utilizing a combination of pre-acid-hydrolysis treatment and activity-guided isolation. Among them, compound 1 (C1) and compound 4 (C4) are newly discovered theasaponins that have not been reported before. The structures of these two new compounds were characterized based on comprehensive 1D and 2D NMR spectroscopy and high-resolution mass spectrometry, as well as data reported in the literature. Secondly, the cytotoxicity and antitumor property of the above four purified compounds were evaluated in selected typical tumor cell lines, Huh-7, HepG2, Hela, A549, and SGC7901, and the results showed that the ED₅₀ value of C4 ranges from 1.5 to 11.3 µM, which is comparable to that of cisplatinum (CDDP) in these five cell lines, indicating that C4 has the most powerful antitumor activity among them. Finally, a preliminary mechanistic investigation was performed to uncover the molecular mechanism underlying the antitumor property of C4, and the results suggested that C4 may trigger apoptosis through the Bcl-2/Caspase-3 and JAK2/STAT3 pathways, and stimulate cell proliferation via the NF-κB/iNOS/COX-2 pathway. Moreover, it was surprising to find that C4 can inhibit the Nrf2/HO-1 pathway, which indicates that C4 has the potency to overcome the resistance to cancer drugs. Therefore, C1 and C4 are two newly identified theasaponin derivatives with antitumor activity from the seed cake of Camellia oleifera, and C4 is a promising antitumor candidate not only for its powerful antitumor activity, but also for its ability to function as an Nrf2 inhibitor to enhance the anticancer drug sensitivity.
Keywords	Camellia oleifera ; antineoplastic activity ; antineoplastic agents ; apoptosis ; cell proliferation ; cytotoxicity ; mass spectrometry ; neoplasm cells ; nuclear magnetic resonance spectroscopy ; oilseed cakes ; triterpenoids ; China
Language	English
Dates of publication	2022-1221
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox12010007
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Hidden in plain sight: Systematic investigation of Leucine-rich repeat containing genes unveil the their regulatory network in response to Fusarium wilt in tung tree.

Cao, Yunpeng / Liu, Meilan / Long, Hongxu / Zhao, Qiuyuan / Jiang, Lan / Zhang, Lin

International journal of biological macromolecules

2020 Volume 163, Page(s) 1759–1767

Abstract	Leucine-rich repeat-containing genes (LRRs) have been reported to play important roles in responses to diseases. However, we poorly understood the response of LRRs to Fusarium wilt infection in tung tree (Vernicia fordii), which is an important economic tree. Here, 437 LRR-containing proteins containing nine types of LRR domains were identified in V. fordii genome. Phylogenetic analysis suggested that nine types of LRR domains could not be divided into separate classes, implying that these LRR domains had a common origin. Totally, 27 LRRs were related to possible resistance to Fusarium wilt after 2, 8, and 13 days post-inoculation. We further found that Vf06G1605 was up-regulate under Fusarium wilt infection after these three time points, Vf10G1602 and Vf02G1413 were up-regulated at 8, and 13 dpi, while Vf07G2320 was down-regulated at these three time points. The WGCNA and promoter elements suggested that WRKY possibly regulate the responses of LRRs to Fusarium wilt infection. This study highlighted the phylogeny and function of LRRs in V. fordii and provided a systematic analysis of these genes in the V. fordii genome. Our results presented here might clearly illustrate physiological mechanisms of resistance to Fusarium wilt infection and the target of marker-assisted breeding in V. fordii.
MeSH term(s)	Aleurites/genetics ; Fusarium/genetics ; Gene Regulatory Networks/genetics ; Genes, Plant/genetics ; Genome, Plant/genetics ; Leucine/genetics ; Phylogeny ; Plant Proteins/genetics ; Promoter Regions, Genetic/genetics ; Protein Domains/genetics ; Up-Regulation/genetics
Chemical Substances	Plant Proteins ; Leucine (GMW67QNF9C)
Language	English
Publishing date	2020-09-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2020.09.106
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.B 2374: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Integrative analysis reveals evolutionary patterns and potential functions of SWEET transporters in Euphorbiaceae

Cao, Yunpeng / Li, Ze / Liu, Meilan / Liu, Wenjuan / Long, Hongxu / Zhang, Lin / Zhao, Qiuyuan / Zhou, Xu

International journal of biological macromolecules. 2019 Oct. 15, v. 139

2019

Abstract: As a macromolecular substance, sucrose contributes to the plant growth and development. SWEET genes, a group of sugar transporters, are a recently found plant gene family and play important roles in sugar efflux, pollen nutrition, nectar secretion, ... ...

Abstract	As a macromolecular substance, sucrose contributes to the plant growth and development. SWEET genes, a group of sugar transporters, are a recently found plant gene family and play important roles in sugar efflux, pollen nutrition, nectar secretion, phloem transport, and seed development. The SWEET genes have been identified and characterized in some plants, but the systematic study in tung tree (Vernicia fordii) was limited. Here, we identified 121 SWEETs in five Euphorbiaceae, and could be divided into four classes with 20 different motifs. Multiple sequence alignment revealed seven transmembrane helixes (TMHs) in the SWEET proteins which were created by an internal duplication of an ancestral three-TMHs unit, connected by TMH4. This study provides direct evidence for the first time for internal duplication in Euphorbiaceae. The large-scale duplication events represented the main driving force for SWEET family expansion in Euphorbiaceae. In addition, we determined the key VfSWEETs for sucrose transport from source to sink tissues in V. fordii and proposed a possible sucrose transport model, which would be helpful for understanding the mechanism of sucrose transport in V. fordii. This study provided a new insight into the evolution, expression and structural variations of SWEETs in V. fordii and four other Euphorbiaceae.
Keywords	genes ; glucose transporters ; growth and development ; nectar secretion ; nutrition ; phloem ; plant growth ; pollen ; protein sweeteners ; seed development ; sequence alignment ; sucrose ; trees ; Vernicia fordii
Language	English
Dates of publication	2019-1015
Size	p. 1-11.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2019.07.102
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.B 2374: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Discovery of New Triterpenoids Extracted from

Wu, Zelong / Tan, Xiaofeng / Zhou, Junqin / Yuan, Jun / Yang, Guliang / Li, Ze / Long, Hongxu / Yi, Yuhang / Lv, Chenghao / Zeng, Chaoxi / Qin, Si

Antioxidants (Basel, Switzerland)

2022 Volume 12, Issue 1

Abstract: Theasaponin derivatives, which are reported to exert antitumor activity, have been widely reported to exist in edible plants, including in the seed cake ... ...

Abstract	Theasaponin derivatives, which are reported to exert antitumor activity, have been widely reported to exist in edible plants, including in the seed cake of
Language	English
Publishing date	2022-12-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox12010007
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: New Insights of Salicylic Acid Into Stamen Abortion of Female Flowers in Tung Tree (

Liu, Meilan / Li, Wenying / Zhao, Guang / Fan, Xiaoming / Long, Hongxu / Fan, Yanru / Shi, Mingwang / Tan, Xiaofeng / Zhang, Lin

Frontiers in genetics

2019 Volume 10, Page(s) 316

Abstract: Tung tree ( ...

Abstract	Tung tree (
Language	English
Publishing date	2019-04-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606823-0
ISSN	1664-8021
ISSN	1664-8021
DOI	10.3389/fgene.2019.00316
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Integrative analysis reveals evolutionary patterns and potential functions of SWEET transporters in Euphorbiaceae.

Cao, Yunpeng / Liu, Wenjuan / Zhao, Qiuyuan / Long, Hongxu / Li, Ze / Liu, Meilan / Zhou, Xu / Zhang, Lin

International journal of biological macromolecules

2019 Volume 139, Page(s) 1–11

Abstract	As a macromolecular substance, sucrose contributes to the plant growth and development. SWEET genes, a group of sugar transporters, are a recently found plant gene family and play important roles in sugar efflux, pollen nutrition, nectar secretion, phloem transport, and seed development. The SWEET genes have been identified and characterized in some plants, but the systematic study in tung tree (Vernicia fordii) was limited. Here, we identified 121 SWEETs in five Euphorbiaceae, and could be divided into four classes with 20 different motifs. Multiple sequence alignment revealed seven transmembrane helixes (TMHs) in the SWEET proteins which were created by an internal duplication of an ancestral three-TMHs unit, connected by TMH4. This study provides direct evidence for the first time for internal duplication in Euphorbiaceae. The large-scale duplication events represented the main driving force for SWEET family expansion in Euphorbiaceae. In addition, we determined the key VfSWEETs for sucrose transport from source to sink tissues in V. fordii and proposed a possible sucrose transport model, which would be helpful for understanding the mechanism of sucrose transport in V. fordii. This study provided a new insight into the evolution, expression and structural variations of SWEETs in V. fordii and four other Euphorbiaceae.
MeSH term(s)	Biological Transport/genetics ; Databases, Genetic ; Euphorbiaceae/genetics ; Euphorbiaceae/metabolism ; Evolution, Molecular ; Genome, Plant/genetics ; Membrane Transport Proteins/genetics ; Membrane Transport Proteins/metabolism ; Nucleotide Motifs ; Phylogeny ; Seeds/metabolism ; Sequence Alignment ; Sucrose/metabolism ; Synteny
Chemical Substances	Membrane Transport Proteins ; Sucrose (57-50-1)
Language	English
Publishing date	2019-07-16
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2019.07.102
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.B 2374: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: The complete chloroplast genome sequence of tung tree (Vernicia fordii): Organization and phylogenetic relationships with other angiosperms.

Li, Ze / Long, Hongxu / Zhang, Lin / Liu, Zhiming / Cao, Heping / Shi, Mingwang / Tan, Xiaofeng

Scientific reports

2017 Volume 7, Issue 1, Page(s) 1869

Abstract: Tung tree (Vernicia fordii) is an economically important tree widely cultivated for industrial oil production in China. To better understand the molecular basis of tung tree chloroplasts, we sequenced and characterized its genome using PacBio RS II ... ...

Abstract	Tung tree (Vernicia fordii) is an economically important tree widely cultivated for industrial oil production in China. To better understand the molecular basis of tung tree chloroplasts, we sequenced and characterized its genome using PacBio RS II sequencing platforms. The chloroplast genome was sequenced with 161,528 bp in length, composed with one pair of inverted repeats (IRs) of 26,819 bp, which were separated by one small single copy (SSC; 18,758 bp) and one large single copy (LSC; 89,132 bp). The genome contains 114 genes, coding for 81 protein, four ribosomal RNAs and 29 transfer RNAs. An expansion with integration of an additional rps19 gene in the IR regions was identified. Compared to the chloroplast genome of Jatropha curcas, a species from the same family, the tung tree chloroplast genome is distinct with 85 single nucleotide polymorphisms (SNPs) and 82 indels. Phylogenetic analysis suggests that V. fordii is a sister species with J. curcas within the Eurosids I. The nucleotide sequence provides vital molecular information for understanding the biology of this important oil tree.
MeSH term(s)	Aleurites/classification ; Aleurites/genetics ; Computational Biology/methods ; Gene Order ; Genome, Chloroplast ; Genomics/methods ; Magnoliopsida/classification ; Magnoliopsida/genetics ; Phylogeny ; Repetitive Sequences, Nucleic Acid ; Reproducibility of Results ; Whole Genome Sequencing
Language	English
Publishing date	2017-05-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-017-02076-6
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Molecular cloning and expression profile of β-ketoacyl-acp synthase gene from tung tree (Vernicia fordii Hemsl.)

Long Hongxu / Tan Xiaofeng / Yan Fangfang / Zhang Lin / Li Ze / Cao Heping

Genetika, Vol 47, Iss 1, Pp 143-

2015 Volume 159

Abstract: Tung tree (Vernicia fordii) is an important woody oil tree. Tung tree seeds contain 50-60% oil with approximately 80 mole α-eleostearic acid (9 cis, 11 trans, 13 trans octadecatrienoic acid). Fatty acid synthesis is catalyzed by the concerted action of ... ...

Abstract	Tung tree (Vernicia fordii) is an important woody oil tree. Tung tree seeds contain 50-60% oil with approximately 80 mole α-eleostearic acid (9 cis, 11 trans, 13 trans octadecatrienoic acid). Fatty acid synthesis is catalyzed by the concerted action of acetyl-CoA carboxylase and fatty acid synthase, a multienzyme complex including β-ketoacyl-acyl-carrier-protein synthase (KAS). Little is known about KAS in tung tree. The objective of this study was to clone KAS genes and analyze their expression profiles in tung tree. A full-length cDNA encoding KAS III and a partial cDNA encoding KAS II were isolated from tung tree by PCR cloning using degenerate primers and rapid amplification of cDNA ends system. The full-length cDNA of VfKAS III was 1881 bp in length with an open reading frame of 1212 bp. VfKAS III genomic DNA was also isolated and sequenced, which contained 8 exons in 5403 bp length. The deduced VfKAS III protein shared approximately 80% identity with homologous KAS IIIs from other plants. Quantitative PCR analysis revealed that KAS II and KAS III were expressed in all of the tissues and organs tested but exhibited different expression patterns in tung tree. The expression levels of KAS II in young tissues were much lower than those in mature tissues, whereas the highest expression levels of KAS III were observed in young stem and young leaf. These results should facilitate further studies on the regulation of tung oil biosynthesis by KAS in tung tree.
Keywords	β-ketoacyl-ACP synthase ; expression profile ; molecular cloning ; Vernicia fordii ; Genetics ; QH426-470 ; Biology (General) ; QH301-705.5 ; Science ; Q
Subject code	571
Language	English
Publishing date	2015-01-01T00:00:00Z
Publisher	Serbian Genetics Society
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED