Article ; Online: Regulation of UV damage repair in quiescent yeast cells.
2020 Volume 90, Page(s) 102861
Abstract: Non-growing quiescent cells face special challenges when repairing lesions produced by exogenous DNA damaging agents. These challenges include the global repression of transcription and translation and a compacted chromatin structure. We investigated how ...
| Abstract | Non-growing quiescent cells face special challenges when repairing lesions produced by exogenous DNA damaging agents. These challenges include the global repression of transcription and translation and a compacted chromatin structure. We investigated how quiescent yeast cells regulated the repair of DNA lesions produced by UV irradiation. We found that UV lesions were excised and repaired in quiescent cells before their re-entry into S phase, and that lesion repair was correlated with high levels of Rad7, a recognition factor in the global genome repair sub-pathway of nucleotide excision repair (GGR-NER). UV exposure led to an increased frequency of mutations that included C->T transitions and T > A transversions. Mutagenesis was dependent on the error-prone translesion synthesis (TLS) DNA polymerase, Pol zeta, which was the only DNA polymerase present in detectable levels in quiescent cells. Across the genome of quiescent cells, UV-induced mutations showed an association with exons that contained H3K36 or H3K79 trimethylation but not with those bound by RNA polymerase II. Together, the data suggest that the distinct physiological state and chromatin structure of quiescent cells contribute to its regulation of UV damage repair. |
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| MeSH term(s) | Cell Cycle ; DNA Damage ; DNA Repair ; DNA, Fungal/metabolism ; DNA, Fungal/radiation effects ; DNA-Binding Proteins/metabolism ; DNA-Directed DNA Polymerase/metabolism ; Mutagenesis ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae/radiation effects ; Saccharomyces cerevisiae Proteins/metabolism ; Ultraviolet Rays |
| Chemical Substances | DNA, Fungal ; DNA-Binding Proteins ; RAD7 protein, S cerevisiae ; Saccharomyces cerevisiae Proteins ; DNA-Directed DNA Polymerase (EC 2.7.7.7) |
| Language | English |
| Publishing date | 2020-04-30 |
| Publishing country | Netherlands |
| Document type | Journal Article |
| ZDB-ID | 2071608-4 |
| ISSN | 1568-7856 ; 1568-7864 |
| ISSN (online) | 1568-7856 |
| ISSN | 1568-7864 |
| DOI | 10.1016/j.dnarep.2020.102861 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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