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Article ; Online: VATS in complicated COVID-19 patients: case series.

Bisagni, P / Armao, F T / Longhi, M / Bastazza, M / Russo, G / Cipolla, G / Pedrazzini, M A / Paglia, S

2022 Volume 75, Issue 3, Page(s) 717–722

Abstract: Common complications of coronavirus disease 2019 (COVID-19) related ARDS and ventilation are barotrauma-induced pneumothorax, pneumatocele and/or empyema. We analysed indications and results of video-assisted thoracoscopic surgery (VATS) in complicated ... ...

Abstract	Common complications of coronavirus disease 2019 (COVID-19) related ARDS and ventilation are barotrauma-induced pneumothorax, pneumatocele and/or empyema. We analysed indications and results of video-assisted thoracoscopic surgery (VATS) in complicated COVID-19 patients. This is a retrospective single-institution study analysing a case series of patients treated by VATS for secondary spontaneous pneumothorax (SSP), pneumatocele and empyema complicating COVID-19, not responding to drainage in Lodi Maggiore Hospital between February 2020 and May 2021. Out of 2076 patients hospitalized in Lodi Maggiore Hospital with COVID-19, nine Males (0,43%; mean age 58,1-33-81) were treated by VATS for complications of pneumonia (6 SSP and 3 empyema; 1 case complicated by haemothorax). 7 patients (77%) had CPAP before surgery for 21.3 days mean (4-38). Mean Operative time was 80.9 min (38-154). Conversion rate was 0%. 3 (33%) patients were admitted to ICU before VATS. Treatments were: bullectomy in six patients (66%), drainage of the pleural space in all patients, pleural decortication and fluid aspiration in five cases (55%). two patients (22%) needed surgery interruption and bilateral ventilation to restore adequate oxygenation. Mortality was 1/9 (11%) due to respiratory failure for persistent pneumonia. In one patient (11%) redo surgery was performed for bleeding. Mean postop Length of Stay (LOS) was 37.9 days (10-77). Our report shows that VATS can be considered an extreme, but effective treatment for COVID-19 patients with SSP, pneumatocele or empyema, for patients who can tolerate general anaesthesia. Attention must be paid to the aerosol-generation of infected droplets.
MeSH term(s)	Male ; Humans ; Thoracic Surgery, Video-Assisted/adverse effects ; Thoracic Surgery, Video-Assisted/methods ; Retrospective Studies ; Empyema, Pleural/etiology ; Empyema, Pleural/surgery ; COVID-19/complications ; Pneumonia/etiology ; Length of Stay
Language	English
Publishing date	2022-11-16
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2572692-4
ISSN	2038-3312 ; 2038-131X
ISSN (online)	2038-3312
ISSN	2038-131X
DOI	10.1007/s13304-022-01420-4
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Zs.B 101: Show issues

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Article ; Online: Pentraxin 3: a novel target in the obesity-inflammation cascade.

Vyas, Vishal / Paula Longhi, Maria

Cardiovascular research

2019 Volume 115, Issue 13, Page(s) 1811–1812

MeSH term(s)	C-Reactive Protein ; Diet ; Humans ; Inflammation ; Obesity ; Serum Amyloid P-Component
Chemical Substances	Serum Amyloid P-Component ; PTX3 protein (148591-49-5) ; C-Reactive Protein (9007-41-4)
Language	English
Publishing date	2019-06-19
Publishing country	England
Document type	Editorial ; Comment
ZDB-ID	80340-6
ISSN	1755-3245 ; 0008-6363
ISSN (online)	1755-3245
ISSN	0008-6363
DOI	10.1093/cvr/cvz146
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Zs.A 565: Show issues

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Article ; Online: Adipose tissue dendritic cells in steady-state.

Macdougall, Claire E / Longhi, M Paula

Immunology

2019 Volume 156, Issue 3, Page(s) 228–234

Abstract: Healthy white adipose tissue (WAT) participates in regulating systemic metabolism, whereas dysfunctional WAT plays a prominent role in the development of obesity-associated co-morbidities. Tissue-resident immune cells are important for maintaining WAT ... ...

Abstract	Healthy white adipose tissue (WAT) participates in regulating systemic metabolism, whereas dysfunctional WAT plays a prominent role in the development of obesity-associated co-morbidities. Tissue-resident immune cells are important for maintaining WAT homeostasis, including conventional dendritic cells (cDCs) which are critical in the initiation and regulation of adaptive immune responses. Due to phenotypic overlap with other myeloid cells, the distinct contribution of WAT cDCs has been poorly understood. This review will discuss the contribution of cDCs in the maintenance of WAT homeostasis. In particular, the review will focus on the metabolic cross-talk between cDCs and adipocytes that regulates local immune responses during physiological conditions.
MeSH term(s)	Adaptive Immunity/immunology ; Adipose Tissue/immunology ; Animals ; Dendritic Cells/immunology ; Homeostasis/immunology ; Humans ; Obesity/immunology
Language	English
Publishing date	2019-01-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	80124-0
ISSN	1365-2567 ; 0019-2805 ; 0953-4954
ISSN (online)	1365-2567
ISSN	0019-2805 ; 0953-4954
DOI	10.1111/imm.13034
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Article ; Online: Cost saving in implementing ERAS protocol in emergency abdominal surgery.

Bisagni, Pietro / D'Abrosca, Vera / Tripodi, Vincenzo / Armao, Francesca Teodora / Longhi, Marco / Russo, Gianluca / Ballabio, Michele

BMC surgery

2024 Volume 24, Issue 1, Page(s) 70

Abstract: Introduction: ERAS (Enhanced Recovery After Surgery) protocol is now proposed as the standard of care in elective major abdominal surgery. Implementation of the ERAS protocol in emergency setting has been proposed but his economic impact has not been ... ...

Abstract	Introduction: ERAS (Enhanced Recovery After Surgery) protocol is now proposed as the standard of care in elective major abdominal surgery. Implementation of the ERAS protocol in emergency setting has been proposed but his economic impact has not been investigated. Aim of this study was to evaluate the cost saving of implementing ERAS in abdominal emergency surgery in a single institution. Methods: A group of 80 consecutive patients treated by ERAS protocol for gastrointestinal emergency surgery in 2021 was compared with an analogue group of 75 consecutive patients treated by the same surgery the year before implementation of ERAS protocol. Adhesion to postoperative items, length of stay, morbidity and mortality were recorded. Cost saving analysis was performed. Results: 50% Adhesion to postoperative items was reached on day 2 in the ERAS group in mean. Laparoscopic approach was 40 vs 12% in ERAS and control group respectively (p ,002). Length of stay was shorter in ERAS group by 3 days (9 vs 12 days p ,002). Morbidity and mortality rate were similar in both groups. The ERAS group had a mean cost saving of 1022,78 € per patient. Conclusions: ERAS protocol implementation in the abdominal emergency setting is cost effective resulting in a significant shorter length of stay and cost saving per patient.
MeSH term(s)	Humans ; Cost Savings ; Postoperative Complications/epidemiology ; Postoperative Complications/prevention & control ; Digestive System Surgical Procedures ; Elective Surgical Procedures/methods ; Enhanced Recovery After Surgery ; Length of Stay
Language	English
Publishing date	2024-02-22
Publishing country	England
Document type	Journal Article
ZDB-ID	2050442-1
ISSN	1471-2482 ; 1471-2482
ISSN (online)	1471-2482
ISSN	1471-2482
DOI	10.1186/s12893-024-02345-y
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Article ; Online: Efficacy of switching from teriparatide to zoledronic acid or denosumab on bone mineral density and biochemical markers of bone turnover in older patients with severe osteoporosis: a real-life study.

Dito, Giorgia / Lugaresi, Marina / Degradi, Chiara / Guabello, Gregorio / Longhi, Matteo / Corbetta, Sabrina

Endocrine

2023 Volume 82, Issue 1, Page(s) 181–189

Abstract: Purpose: Osteoporosis is characterized by loss of bone mass and susceptibility to fracture. Skeletal effects of teriparatide (TPT) are not persistent after drug withdrawal and sequential therapy with bisphosphonates or denosumab (Dmab) after TPT ... ...

Abstract	Purpose: Osteoporosis is characterized by loss of bone mass and susceptibility to fracture. Skeletal effects of teriparatide (TPT) are not persistent after drug withdrawal and sequential therapy with bisphosphonates or denosumab (Dmab) after TPT discontinuation represents a valid option. Here, the two sequential strategies were evaluated in severe osteoporotic patients. Methods: The study retrospectively enrolled 56 severe osteoporotic patients who received TPT for 24 months followed by 24 months of zoledronic acid (ZOL) (TPT + ZOL) or Dmab (TPT+Dmab). Clinical features, incident fractures, bone mineral density (BMD) measurements, and bone marker profiles were collected. One-way ANOVA analyzed the difference between mean T-scores at baseline, after 24 months of TPT, and after 2 doses of ZOL or after at least 3 doses of Dmab. Results: Twenty-three patients received TPT + ZOL (19 females, 4 males; median [IR] age, 74.3 [66.9, 78.6] years) and 33 patients received TPT+Dmab (31 females, 2 males; mean [IR] age, 66.6 ± 11.3 years). Mean lumbar and hip T-scores were increased after both TPT + ZOL and TPT+Dmab (all p < 0.05 vs baseline). The size effects induced by TPT + ZOL on the lumbar and hip BMD T-scores were similar to those observed with TPT+Dmab with mean T-scores increases of about 1 and 0.4 SD, respectively. No significant between-group differences were identified. Incident fragility fractures occurred in 3 (13%) patients treated with TPT + ZOL and in 5 (15%) patients treated with TPT+Dmab. Conclusions: Sequential TPT + ZOL therapy is likely to increase bone mineralization at the lumbar level and to stabilize it at the femoral level, similarly to what obtained with the sequential TPT+Dmab. Both ZOL and Dmab are suggested to be effective sequential treatments after TPT.
MeSH term(s)	Male ; Female ; Humans ; Aged ; Middle Aged ; Zoledronic Acid/therapeutic use ; Zoledronic Acid/pharmacology ; Teriparatide/adverse effects ; Bone Density ; Denosumab/adverse effects ; Bone Density Conservation Agents/adverse effects ; Retrospective Studies ; Osteoporosis/drug therapy ; Osteoporosis/chemically induced ; Diphosphonates/adverse effects ; Fractures, Bone/chemically induced ; Bone Remodeling ; Biomarkers
Chemical Substances	Zoledronic Acid (6XC1PAD3KF) ; Teriparatide (10T9CSU89I) ; Denosumab (4EQZ6YO2HI) ; Bone Density Conservation Agents ; Diphosphonates ; Biomarkers
Language	English
Publishing date	2023-07-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	1194484-5
ISSN	1559-0100 ; 1355-008X ; 0969-711X
ISSN (online)	1559-0100
ISSN	1355-008X ; 0969-711X
DOI	10.1007/s12020-023-03431-6
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Zs.A 3884: Show issues

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Article ; Online: Clinical, biochemical and genetic findings in adult patients with chronic hypophosphatasemia.

Guarnieri, V / Sileri, F / Indirli, R / Guabello, G / Longhi, M / Dito, G / Verdelli, C / Corbetta, S

Journal of endocrinological investigation

2021 Volume 45, Issue 1, Page(s) 125–137

Abstract: Purpose: The study aimed to define the clinical, biochemical and genetic features of adult patients with osteopenia/osteoporosis and/or bone fragility and low serum alkaline phosphatase (sALP).: Methods: Twenty-two patients with at least two sALP ... ...

Abstract	Purpose: The study aimed to define the clinical, biochemical and genetic features of adult patients with osteopenia/osteoporosis and/or bone fragility and low serum alkaline phosphatase (sALP). Methods: Twenty-two patients with at least two sALP values below the reference range were retrospectively enrolled after exclusion of secondary causes. Data about clinical features, mineral and bone markers, serum pyridoxal-5'-phosphate (PLP), urine phosphoethanolamine (PEA), lumbar and femur bone densitometry, and column X-ray were collected. Peripheral blood DNA of each participant was analyzed to detect ALPL gene anomalies. Results: Pathogenic ALPL variants (pALPL) occurred in 23% and benign variants in 36% of patients (bALPL), while nine patients harbored wild-type alleles (wtALPL). Fragility fractures and dental anomalies were more frequent in patients harboring pALPL and bALPL than in wtALPL patients. Of note, wtALPL patients comprised women treated with tamoxifen for hormone-sensitive breast cancer. Mineral and bone markers were similar in the three groups. Mean urine PEA levels were significantly higher in patients harboring pALPL than those detected in patients harboring bALPL and wtALPL; by contrast, serum PLP levels were similar in the three groups. A 6-points score, considering clinical and biochemical features, was predictive of pALPL detection [P = 0.060, OR 1.92 (95% CI 0.972, 3.794)], and more significantly of pALPL or bALPL [P = 0.025, OR 14.33 (95% CI 1.401, 14.605)]. Conclusion: In osteopenic/osteoporotic patients, single clinical or biochemical factors did not distinguish hypophosphatasemic patients harboring pALPL or bALPL from those harboring wtALPL. Occurrence of multiple clinical and biochemical features is predictive of ALPL anomalies, and, therefore, they should be carefully identified. Tamoxifen emerged as a hypophosphatasemic drug.
MeSH term(s)	Alkaline Phosphatase/analysis ; Alkaline Phosphatase/blood ; Alkaline Phosphatase/genetics ; Biomarkers/analysis ; Biomarkers/blood ; Bone Density ; Bone Diseases, Metabolic/blood ; Bone Diseases, Metabolic/epidemiology ; Bone Diseases, Metabolic/genetics ; Chronic Disease ; Cross-Sectional Studies ; DNA Mutational Analysis ; Female ; Fractures, Bone/blood ; Fractures, Bone/epidemiology ; Fractures, Bone/genetics ; Humans ; Hypophosphatemia/blood ; Hypophosphatemia/diagnosis ; Hypophosphatemia/epidemiology ; Hypophosphatemia/genetics ; Italy/epidemiology ; Male ; Middle Aged ; Osteoporosis/blood ; Osteoporosis/epidemiology ; Osteoporosis/genetics ; Polymorphism, Single Nucleotide ; Pyridoxal Phosphate/analysis ; Pyridoxal Phosphate/blood ; Retrospective Studies
Chemical Substances	Biomarkers ; Pyridoxal Phosphate (5V5IOJ8338) ; ALPL protein, human (EC 3.1.3.1) ; Alkaline Phosphatase (EC 3.1.3.1)
Language	English
Publishing date	2021-07-02
Publishing country	Italy
Document type	Journal Article
ZDB-ID	432272-1
ISSN	1720-8386 ; 0391-4097 ; 1121-1369
ISSN (online)	1720-8386
ISSN	0391-4097 ; 1121-1369
DOI	10.1007/s40618-021-01625-1
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Article ; Online: Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications.

Hearnden, Robert / Sandhar, Balraj / Vyas, Vishal / Longhi, M Paula

STAR protocols

2021 Volume 2, Issue 2, Page(s) 100422

Abstract: This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the ... ...

Abstract	This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-resident cell populations. For complete details on the use and execution of this protocol, please refer to Macdougall et al. (2018).
MeSH term(s)	Adipose Tissue/cytology ; Animals ; Cell Culture Techniques/methods ; Cell Separation/methods ; Cells, Cultured ; Female ; Humans ; Male ; Mice ; Stromal Vascular Fraction/physiology
Language	English
Publishing date	2021-03-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2021.100422
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Article: Hypercalciuria in Postmenopausal Women With Reduced Bone Mineral Density Is Associated With Different Mineral Metabolic Profiles: Effects of Treatment With Thiazides and Anti-resorptives.

Nicoli, Federico / Dito, Giorgia / Guabello, Gregorio / Longhi, Matteo / Corbetta, Sabrina

Frontiers in medicine

2021 Volume 8, Page(s) 780087

Abstract: Hypercalciuria may represent a challenge during the workup for osteoporosis management. The present study aimed: (1) to describe the phenotype associated with hypercalciuria in vitamin D-sufficient (serum 25 hydroxyvitamin D (25OHD) > 20 ng/ml) patients ... ...

Abstract	Hypercalciuria may represent a challenge during the workup for osteoporosis management. The present study aimed: (1) to describe the phenotype associated with hypercalciuria in vitamin D-sufficient (serum 25 hydroxyvitamin D (25OHD) > 20 ng/ml) patients with osteopenia/osteoporosis; (2) to analyze the effects of thiazides and anti-resorptive drugs on urine calcium excretion (UCa), mineral metabolic markers, and bone mineral density. Seventy-seven postmenopausal women with hypercalciuria (Uca > 4.0 mg/kg body weight/24 h on two determinations) were retrospectively evaluated in a real-life setting. Median UCa was 5.39 (4.75-6.70) mg/kg/24 h. Kidney stones occurred in 32.9% of patients, who had median UCa similar to that of patients without kidney stones. Clustering analysis considering the three variables, such as serum calcium, phosphate, and parathormone (PTH), identified two main clusters of hypercalciuric patients. Cluster 1 (
Language	English
Publishing date	2021-12-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2775999-4
ISSN	2296-858X
ISSN	2296-858X
DOI	10.3389/fmed.2021.780087
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Article ; Online: Development of self-microemulsifying lipid-based formulations of

Aloisio, Carolina / Shah, Ankita V / Longhi, Marcela / Serajuddin, Abu T M

Drug development and industrial pharmacy

2021 Volume 47, Issue 6, Page(s) 897–907

Abstract: The aim of this work was to develop self-microemulsifying lipid-based formulations ... ...

Abstract	The aim of this work was to develop self-microemulsifying lipid-based formulations of
MeSH term(s)	Chemistry, Pharmaceutical ; Drug Delivery Systems ; Emulsions ; Lipids ; Particle Size ; Resveratrol ; Solubility ; Surface-Active Agents ; Water
Chemical Substances	Emulsions ; Lipids ; Surface-Active Agents ; Water (059QF0KO0R) ; Resveratrol (Q369O8926L)
Language	English
Publishing date	2021-07-16
Publishing country	England
Document type	Journal Article
ZDB-ID	751874-2
ISSN	1520-5762 ; 0363-9045
ISSN (online)	1520-5762
ISSN	0363-9045
DOI	10.1080/03639045.2021.1934866
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Zs.A 1335: Show issues

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Article ; Online: Identification of biomarkers in patients with rheumatoid arthritis responsive to DMARDs but with progressive bone erosion.

Marasco, Emiliano / Fabbriciani, Gianluigi / Rotunno, Laura / Longhi, Matteo / De Luca, Paola / de Girolamo, Laura / Colombini, Alessandra

Frontiers in immunology

2023 Volume 14, Page(s) 1254139

Abstract: Introduction: Rheumatoid arthritis (RA) is an inflammatory autoimmune disease that may cause joint destruction and disability. The pharmacological treatment of RA aims at obtaining disease remission by effectively ceasing joint inflammation and ... ...

Abstract	Introduction: Rheumatoid arthritis (RA) is an inflammatory autoimmune disease that may cause joint destruction and disability. The pharmacological treatment of RA aims at obtaining disease remission by effectively ceasing joint inflammation and arresting progressive bone erosions. Some patients present bone lesions accrual even after controlling joint inflammation with current therapies. Our study aimed to analyze lymphocyte subsets and levels of circulating cytokines in patients with RA with progressive bone erosions. Methods: We enrolled 20 patients with a diagnosis of RA and 12 healthy donors (HD). Patients with RA were divided into patients with bone erosions (RA-BE+) and without bone erosions (RA-BE-). Lymphocyte subsets in peripheral blood were evaluated by flow cytometry. Circulating cytokines levels were evaluated by protein array. Results: The distribution of lymphocyte subsets was not able to separate HD from AR patients and RA-BE+ and RA-BE- in cluster analysis. We observed a significant expansion of CXCR5 Conclusion: We show that patients with progressive erosive disease are characterized by abnormalities in B cells and in cytokines with a proven role in bone reabsorption. Understanding the role played by B cells and the cytokine IL-11 and IL-17A in progressive erosive disease can help identify novel biomarkers of erosive disease and design treatment approaches aimed at halting joint damage in RA.
MeSH term(s)	Humans ; Interleukin-11 ; Interleukin-17 ; Arthritis, Rheumatoid/diagnosis ; Arthritis, Rheumatoid/drug therapy ; Antirheumatic Agents/pharmacology ; Antirheumatic Agents/therapeutic use ; Biomarkers ; Cytokines ; Inflammation/drug therapy
Chemical Substances	Interleukin-11 ; Interleukin-17 ; Antirheumatic Agents ; Biomarkers ; Cytokines
Language	English
Publishing date	2023-09-19
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.1254139
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