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  1. Article ; Online: Potential to Use Fingerprints for Monitoring Therapeutic Levels of Isoniazid and Treatment Adherence.

    Ismail, Mahado / Costa, Catia / Longman, Katherine / Chambers, Mark A / Menzies, Sarah / Bailey, Melanie J

    ACS omega

    2022  Volume 7, Issue 17, Page(s) 15167–15173

    Abstract: A fingerprint offers a convenient, noninvasive sampling matrix for monitoring therapeutic drug use. However, a barrier to widespread adoption of fingerprint sampling is the fact that the sample volume is uncontrolled. Fingerprint samples ( ...

    Abstract A fingerprint offers a convenient, noninvasive sampling matrix for monitoring therapeutic drug use. However, a barrier to widespread adoption of fingerprint sampling is the fact that the sample volume is uncontrolled. Fingerprint samples (
    Language English
    Publishing date 2022-04-21
    Publishing country United States
    Document type Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.2c01257
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Changes to the sebum lipidome upon COVID-19 infection observed via rapid sampling from the skin.

    Spick, Matt / Longman, Katherine / Frampas, Cecile / Lewis, Holly / Costa, Catia / Walters, Deborah Dunn / Stewart, Alex / Wilde, Michael / Greener, Danni / Evetts, George / Trivedi, Drupad / Barran, Perdita / Pitt, Andy / Bailey, Melanie

    EClinicalMedicine

    2021  Volume 33, Page(s) 100786

    Abstract: Background: The COVID-19 pandemic has led to an unprecedented demand for testing - for diagnosis and prognosis - as well as for investigation into the impact of the disease on the host metabolism. Sebum sampling has the potential to support both needs ... ...

    Abstract Background: The COVID-19 pandemic has led to an unprecedented demand for testing - for diagnosis and prognosis - as well as for investigation into the impact of the disease on the host metabolism. Sebum sampling has the potential to support both needs by looking at what the virus does to us, rather than looking for the virus itself.
    Methods: In this pilot study, sebum samples were collected from 67 hospitalised patients (30 COVID-19 positive and 37 COVID-19 negative) by gauze swab. Lipidomics analysis was carried out using liquid chromatography mass spectrometry, identifying 998 reproducible features. Univariate and multivariate statistical analyses were applied to the resulting feature set.
    Findings: Lipid levels were depressed in COVID-19 positive participants, indicative of dyslipidemia;
    Interpretation: COVID-19 dysregulates many areas of metabolism; in this work we show that the skin lipidome can be added to the list. Given that samples can be provided quickly and painlessly, we conclude that sebum is worthy of future consideration for clinical sampling.
    Funding: The authors acknowledge funding from the EPSRC Impact Acceleration Account for sample collection and processing, as well as EPSRC Fellowship Funding EP/R031118/1, the University of Surrey and BBSRC BB/T002212/1. Mass Spectrometry was funded under EP/P001440/1.
    Language English
    Publishing date 2021-03-06
    Publishing country England
    Document type Journal Article
    ISSN 2589-5370
    ISSN (online) 2589-5370
    DOI 10.1016/j.eclinm.2021.100786
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Paper spray screening and liquid chromatography/mass spectrometry confirmation for medication adherence testing: A two-step process.

    Costa, Catia / Frampas, Cecile / Longman, Katherine A / Palitsin, Vladimir / Ismail, Mahado / Sears, Patrick / Nilforooshan, Ramin / Bailey, Melanie J

    Rapid communications in mass spectrometry : RCM

    2019  

    Abstract: Rationale: Paper spray offers a rapid screening test without the need for sample preparation. The incomplete extraction of paper spray allows for further testing using more robust, selective and sensitive techniques such as liquid chromatography/mass ... ...

    Abstract Rationale: Paper spray offers a rapid screening test without the need for sample preparation. The incomplete extraction of paper spray allows for further testing using more robust, selective and sensitive techniques such as liquid chromatography/mass spectrometry (LC/MS). Here we develop a two-step process of paper spray followed by LC/MS to (1) rapidly screen a large number of samples and (2) confirm any disputed results. This demonstrates the applicability for testing medication adherence from a fingerprint.
    Methods: Following paper spray analysis, drugs of abuse samples were analysed using LC/MS. All analyses were completed using a Q Exactive™ Plus Orbitrap™ mass spectrometer. This two-step procedure was applied to fingerprints collected from patients on a maintained dose of the antipsychotic drug quetiapine.
    Results: The extraction efficiency of paper spray for two drugs of abuse and metabolites was found to be between 15 and 35% (analyte dependent). For short acquisition times, the extraction efficiency was found to vary between replicates by less than 30%, enabling subsequent analysis by LC/MS. This two-step process was then applied to fingerprints collected from two patients taking the antipsychotic drug quetiapine, which demonstrates how a negative screening result from paper spray can be resolved using LC/MS.
    Conclusions: We have shown for the first time the sequential analysis of the same sample using paper spray and LC/MS, as well as the detection of an antipsychotic drug from a fingerprint. We propose that this workflow may also be applied to any type of sample compatible with paper spray, and will be especially convenient where only one sample is available for analysis.
    Language English
    Publishing date 2019-08-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 58731-x
    ISSN 1097-0231 ; 0951-4198
    ISSN (online) 1097-0231
    ISSN 0951-4198
    DOI 10.1002/rcm.8553
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Pandemic, Epidemic, Endemic: B Cell Repertoire Analysis Reveals Unique Anti-Viral Responses to SARS-CoV-2, Ebola and Respiratory Syncytial Virus.

    Stewart, Alexander / Sinclair, Emma / Ng, Joseph Chi-Fung / O'Hare, Joselli Silva / Page, Audrey / Serangeli, Ilaria / Margreitter, Christian / Orsenigo, Federica / Longman, Katherine / Frampas, Cecile / Costa, Catia / Lewis, Holly-May / Kasar, Nora / Wu, Bryan / Kipling, David / Openshaw, Peter Jm / Chiu, Christopher / Baillie, J Kenneth / Scott, Janet T /
    Semple, Malcolm G / Bailey, Melanie J / Fraternali, Franca / Dunn-Walters, Deborah K

    Frontiers in immunology

    2022  Volume 13, Page(s) 807104

    Abstract: Immunoglobulin gene heterogeneity reflects the diversity and focus of the humoral immune response towards different infections, enabling inference of B cell development processes. Detailed compositional and lineage analysis of long read IGH repertoire ... ...

    Abstract Immunoglobulin gene heterogeneity reflects the diversity and focus of the humoral immune response towards different infections, enabling inference of B cell development processes. Detailed compositional and lineage analysis of long read IGH repertoire sequencing, combining examples of pandemic, epidemic and endemic viral infections with control and vaccination samples, demonstrates general responses including increased use of
    MeSH term(s) Antibodies, Viral ; COVID-19 ; Ebolavirus ; Hemorrhagic Fever, Ebola ; Humans ; Pandemics ; Respiratory Syncytial Virus, Human/genetics ; SARS-CoV-2
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2022-05-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2022.807104
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Pandemic, epidemic, endemic: B cell repertoire analysis reveals unique anti-viral responses to SARS-CoV-2, Ebola and Respiratory Syncytial Virus

    Stewart, Alexander / Sinclair, Emma / Ng, Joseph / O’Hare, Joselli Silvia / Page, Audrey / Serangeli, Ilaria / Margreitter, Christian / Kasar, Nora / Longman, Katherine / Frampas, Cecile / Costa, Catia / Lewis, Holly / Wu, Bryan / Kipling, David / Openshaw, Peter / Chu, Christopher / Baillie, J Kenneth / Scott, Janet T / Semple, Malcolm G /
    Bailey, Melanie / Fraternali, Franca / Dunn-Walters, Deborah

    bioRxiv

    Abstract: Immunoglobulin gene heterogeneity reflects the diversity and focus of the humoral immune response towards different infections, enabling inference of B cell development processes. Detailed compositional and lineage analysis of long read IGH repertoire ... ...

    Abstract Immunoglobulin gene heterogeneity reflects the diversity and focus of the humoral immune response towards different infections, enabling inference of B cell development processes. Detailed compositional and lineage analysis of long read IGH repertoire sequencing, combining examples of pandemic, epidemic and endemic viral infections with control and vaccination samples, demonstrates general responses including increased use of IGHV4-39 in both EBOV and COVID-19 infection cohorts. We also show unique characteristics absent in RSV infection or yellow fever vaccine samples: EBOV survivors show unprecedented high levels of class switching events while COVID-19 repertoires from acute disease appear underdeveloped. Despite the high levels of clonal expansion in COVID-19 IgG1 repertoires there is a striking lack of evidence of germinal centre mutation and selection. Given the differences in COVID-19 morbidity and mortality with age, it is also pertinent that we find significant differences in repertoire characteristics between young and old patients. Our data supports the hypothesis that a primary viral challenge can result in a strong but immature humoral response where failures in selection of the repertoire risks off-target effects.
    Keywords covid19
    Language English
    Publishing date 2021-08-19
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2021.08.19.456951
    Database COVID19

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  6. Article ; Online: Pandemic, epidemic, endemic: B cell repertoire analysis reveals unique anti-viral responses to SARS-CoV-2, Ebola and Respiratory Syncytial Virus

    Stewart, Alexander / Sinclair, Emma / Ng, Joseph CF / Fraternali, Franca / Silvia-O'Hare, Joselli / Page, Audrey / Serangeli, Ilaria / Margreitter, Christian / Kasar, Nora / Longman, Katherine / Frampas, Cecile / Lewis, Holly-May / Costa, Catia / Wu, Bryan / Kipling, David / Openshaw, Peter / Chiu, Christopher / Baillie, J Kenneth / Scott, Janet /
    Semple, Malcolm / Bailey, Melanie / Dunn-Walters, Deborah

    bioRxiv

    Abstract: Immunoglobulin gene heterogeneity reflects the diversity and focus of the humoral immune response towards different infections, enabling inference of B cell development processes. Detailed compositional and lineage analysis of long read IGH repertoire ... ...

    Abstract Immunoglobulin gene heterogeneity reflects the diversity and focus of the humoral immune response towards different infections, enabling inference of B cell development processes. Detailed compositional and lineage analysis of long read IGH repertoire sequencing, combining examples of pandemic, epidemic and endemic viral infections with control and vaccination samples, demonstrates general responses including increased use of IGHV4-39 in both EBOV and COVID-19 infection cohorts. We also show unique characteristics absent in RSV infection or yellow fever vaccine samples: EBOV survivors show unprecedented high levels of class switching events while COVID-19 repertoires from acute disease appear underdeveloped. Despite the high levels of clonal expansion in COVID-19 IgG1 repertoires there is a striking lack of evidence of germinal centre mutation and selection. Given the differences in COVID-19 morbidity and mortality with age, it is also pertinent that we find significant differences in repertoire characteristics between young and old patients. Our data supports the hypothesis that a primary viral challenge can result in a strong but immature humoral response where failures in selection of the repertoire risks off-target effects.
    Keywords covid19
    Language English
    Publishing date 2021-08-19
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2021.08.19.456951
    Database COVID19

    Full text online

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