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  1. Article ; Online: Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada.

    Boodman, Carl / Loomer, Courtney / Dibernardo, Antonia / Hatchette, Todd / LeBlanc, Jason J / Waitt, Brooks / Lindsay, L Robbin

    Emerging infectious diseases

    2023  Volume 29, Issue 1, Page(s) 175–178

    Abstract: Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%-95.8% sensitivity and 2.8 average cycle threshold elevation. ... ...

    Abstract Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%-95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma spp. when whole blood is unavailable.
    MeSH term(s) Animals ; Humans ; Anaplasmosis/diagnosis ; Real-Time Polymerase Chain Reaction ; Anaplasma phagocytophilum/genetics ; Canada/epidemiology
    Language English
    Publishing date 2023-01-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1380686-5
    ISSN 1080-6059 ; 1080-6040
    ISSN (online) 1080-6059
    ISSN 1080-6040
    DOI 10.3201/eid2901.220988
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4778: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Vector Competence of Some Mosquito Species From Canada For Zika Virus.

    Dibernardo, Antonia / Turell, Michael J / Lindsay, L Robbin / Loomer, Courtney / Iranpour, Mahmood

    Journal of the American Mosquito Control Association

    2018  Volume 33, Issue 4, Page(s) 276–281

    Abstract: The recent introduction of Zika virus (ZIKV) into the Americas and the occurrence of birth defects associated with infection during pregnancy have created a concern about the spread of this virus into more northern countries in the Americas. Therefore, ... ...

    Abstract The recent introduction of Zika virus (ZIKV) into the Americas and the occurrence of birth defects associated with infection during pregnancy have created a concern about the spread of this virus into more northern countries in the Americas. Therefore, we examined several species of mosquitoes found in southern Manitoba, Canada, for their susceptibility to infection and their ability to transmit ZIKV. Aedes cinereus, Ae. euedes, Ae. fitchii, Ae. sticticus, Ae. vexans, Coquillettidia perturbans, Culex restuans, and Cx. tarsalis were captured in the vicinity of Winnipeg, Manitoba; brought to the laboratory; and allowed to feed on a ZIKV-sheep blood suspension to determine oral susceptibility. In addition, some of the nonfed individuals were inoculated intrathoracically to examine for the presence of a salivary gland barrier. Despite ingesting blood containing 10
    MeSH term(s) Aedes/virology ; Animals ; Canada ; Culex/virology ; Culicidae/virology ; Mosquito Vectors/virology ; Zika Virus/classification ; Zika Virus/physiology
    Language English
    Publishing date 2018-01-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 632618-3
    ISSN 1943-6270 ; 8756-971X
    ISSN (online) 1943-6270
    ISSN 8756-971X
    DOI 10.2987/17-6664.1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2810: Show issues Location:
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  3. Article ; Online: Jamestown Canyon and snowshoe hare virus seroprevalence in New Brunswick.

    Mincer, Jacqueline / Materniak, Stefanie / Dimitrova, Kristina / Wood, Heidi / Iranpour, Mahmood / Dibernardo, Antonia / Loomer, Courtney / Drebot, Michael A / Lindsay, L Robbin / Webster, Duncan

    Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada

    2021  Volume 6, Issue 3, Page(s) 213–220

    Abstract: Background: Jamestown Canyon virus (JCV) and snowshoe hare virus (SSHV) are wide-ranging mosquito-borne arboviruses in the California serogroup viruses (CSGV) that are known to circulate in New Brunswick. Despite potential for debilitating central ... ...

    Abstract Background: Jamestown Canyon virus (JCV) and snowshoe hare virus (SSHV) are wide-ranging mosquito-borne arboviruses in the California serogroup viruses (CSGV) that are known to circulate in New Brunswick. Despite potential for debilitating central nervous system manifestations, the prevalence of human exposure to these viruses in New Brunswick is unknown. The goal of this study was to quantify rates of human exposure in New Brunswick to these neglected arboviruses.
    Methods: A retrospective, anonymized provincial serosurvey was performed using a stratified random sample of residual sera submitted between May 2015 and August 2016. To determine the seroprevalence of JCV and SSHV, competitive enzyme-linked immunosorbent assay-positive samples were confirmed positive using plaque-reduction neutralization testing (PRNT).
    Results: A total of 452 serum samples were screened. The seroprevalence of antibodies against CSGV was estimated to be 31.6% (95% CI 27.4% to 36.1%) with 143 positive samples. PRNT results indicated that most single virus exposures were due to JCV (38 of 143; 26.6%) rather than SSHV (3 of 143; 2.1%). The species of CSGV, to which the remaining 102 seropositive people were exposed, could not be precisely determined.
    Conclusions: The prevalence of human exposure to CSGV is high but comparable to rates observed in other Atlantic Canadian jurisdictions. Studies such as this provide important baseline epidemiological data regarding the risk of exposure to these neglected arboviruses. SSHV and JCV should be considered in the differential diagnosis for undiagnosed febrile and neuroinvasive illness during mosquito season, particularly when testing for common aetiologies is negative or inconclusive.
    Language English
    Publishing date 2021-09-30
    Publishing country Canada
    Document type Journal Article
    ISSN 2371-0888
    ISSN (online) 2371-0888
    DOI 10.3138/jammi-2021-0009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human clinical disease and an emended description of Corynebacterium mastitidis.

    Bernard, Kathryn A / Pacheco, Ana Luisa / Loomer, Courtney / Burdz, Tamara / Wiebe, Deborah / Huynh, Chris / Kaplen, Brynn / Olson, Adam B / Cnockaert, Margo / Eguchi, Hiroshi / Kuwahara, Tomomi / Nakayama-Imaohji, Haruyuki / Shiota, Hiroshi / Boudewijns, Michael / Van Hoecke, Frederik / Vandamme, Peter

    International journal of systematic and evolutionary microbiology

    2016  Volume 66, Issue 8, Page(s) 2803–2812

    Abstract: Strains of members of the genus Corynebacterium derived from ophthalmologic patients in Japan, Belgium and Switzerland and found to be closely related to-, but distinguishable from Corynebacterium mastitidis by 16S rRNA gene sequencing, were ... ...

    Abstract Strains of members of the genus Corynebacterium derived from ophthalmologic patients in Japan, Belgium and Switzerland and found to be closely related to-, but distinguishable from Corynebacterium mastitidis by 16S rRNA gene sequencing, were characterized using biochemical, chemotaxonomic, MALDI-TOF mass spectrometry and antimicrobial susceptibility methods and DNA-DNA hybridization as well as by whole-genome sequencing (WGS). Based on this investigation, we describe Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human ocular specimens, as well as emend the description of Corynebacterium mastitidis. Type strains for these species are: C. lowii R-50085T (=LMG 28276T =CCUG 65815T) and C. oculi R-50187T (=LMG 28277T =CCUG 65816T). DNA G+C content was found to be 62.2 % (by HPLC) and 62.8 % (by WGS) for C. lowii R-50085T, 64.1 % (HPLC) and 64.8 % (WGS) for C. oculi R-50187T and 67.8 % (HPLC) for C. mastitidis LMG 19040T [=S-8T =CCUG 38654T =CECT 4843T =CIP 105509T =DSM 44356T =IFO (NBRC)16160T =JCM 12269T].
    MeSH term(s) Bacterial Typing Techniques ; Base Composition ; Belgium ; Corynebacterium/classification ; Corynebacterium/genetics ; Corynebacterium/isolation & purification ; DNA, Bacterial/genetics ; Eye/microbiology ; Fatty Acids/chemistry ; Humans ; Japan ; Nucleic Acid Hybridization ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA ; Switzerland
    Chemical Substances DNA, Bacterial ; Fatty Acids ; RNA, Ribosomal, 16S
    Language English
    Publishing date 2016-04-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 2002336-4
    ISSN 1466-5034 ; 1466-5026
    ISSN (online) 1466-5034
    ISSN 1466-5026
    DOI 10.1099/ijsem.0.001059
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 409: Show issues Location:
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    ab Jg. 2022: Lesesaal (EG)

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