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  1. Book ; Online: Identification of SARS-CoV-2 and additional respiratory pathogens cases under the investigation of COVID-19 initial phase in a Brazilian reference laboratory

    Matos, Aline da Rocha / Motta, Fernando Couto / Caetano, Braulia Costa / Ogrzewalska, Maria / Garcia, Cristiana Couto / Lopes, Jonathan Christian Oliveira / Miranda, Milene / Livorati, Miriam Teresinha Furlam Prando / Abreu, André / Brown, David / Siqueira, Marilda Mendonça

    Memórias do Instituto Oswaldo Cruz v.115 2020

    2020  

    Abstract: Coronavirus disease 2019 (COVID-19) surveillance, in Brazil, initiated shortly after its description, in China. Our aim was to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and additional pathogens in samples from the initial phase ... ...

    Abstract Coronavirus disease 2019 (COVID-19) surveillance, in Brazil, initiated shortly after its description, in China. Our aim was to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and additional pathogens in samples from the initial phase of the outbreak in Brazil, from late February to late March. From 707 samples analysed, 29 (4.1%) were SARS-CoV-2 positive. Fever and cough were their most prevalent symptoms. Co-detection of rhinovirus was observed in 2 (6.9%) cases. Additional pathogens were identified in 66.1% of the SARS-CoV-2 negative cases, mainly rhinovirus and influenza A(H1N1)pdm09. Thus, we emphasise the importance of differential diagnosis in COVID-19 suspected cases.
    Keywords SARS-CoV-2 ; COVID-19 ; surveillance ; laboratory ; covid19
    Language English
    Publishing date 2020-01-01
    Publisher Instituto Oswaldo Cruz, Ministério da Saúde
    Publishing country br
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Identification of SARS-CoV-2 and additional respiratory pathogens cases under the investigation of COVID-19 initial phase in a Brazilian reference laboratory.

    Matos, Aline da Rocha / Motta, Fernando Couto / Caetano, Braulia Costa / Ogrzewalska, Maria / Garcia, Cristiana Couto / Lopes, Jonathan Christian Oliveira / Miranda, Milene / Livorati, Miriam Teresinha Furlam Prando / Abreu, André / Brown, David / Siqueira, Marilda Mendonça

    Memorias do Instituto Oswaldo Cruz

    2020  Volume 115, Page(s) e200232

    Abstract: Coronavirus disease 2019 (COVID-19) surveillance, in Brazil, initiated shortly after its description, in China. Our aim was to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and additional pathogens in samples from the initial phase ... ...

    Abstract Coronavirus disease 2019 (COVID-19) surveillance, in Brazil, initiated shortly after its description, in China. Our aim was to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and additional pathogens in samples from the initial phase of the outbreak in Brazil, from late February to late March. From 707 samples analysed, 29 (4.1%) were SARS-CoV-2 positive. Fever and cough were their most prevalent symptoms. Co-detection of rhinovirus was observed in 2 (6.9%) cases. Additional pathogens were identified in 66.1% of the SARS-CoV-2 negative cases, mainly rhinovirus and influenza A(H1N1)pdm09. Thus, we emphasise the importance of differential diagnosis in COVID-19 suspected cases.
    MeSH term(s) Betacoronavirus/isolation & purification ; Brazil/epidemiology ; COVID-19 ; China ; Coronavirus Infections/diagnosis ; Coronavirus Infections/epidemiology ; Diagnosis, Differential ; Humans ; Influenza A Virus, H1N1 Subtype/isolation & purification ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/epidemiology ; Rhinovirus/isolation & purification ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-09-18
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 953293-6
    ISSN 1678-8060 ; 0074-0276
    ISSN (online) 1678-8060
    ISSN 0074-0276
    DOI 10.1590/0074-02760200232
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Identification of SARS-CoV-2 and additional respiratory pathogens cases under the investigation of COVID-19 initial phase in a Brazilian reference laboratory

    Matos, Aline da Rocha / Motta, Fernando Couto / Caetano, Braulia Costa / Ogrzewalska, Maria / Garcia, Cristiana Couto / Lopes, Jonathan Christian Oliveira / Miranda, Milene / Livorati, Miriam Teresinha Furlam Prando / Abreu, André / Brown, David / Siqueira, Marilda Mendonça

    Mem Inst Oswaldo Cruz

    Abstract: Coronavirus disease 2019 (COVID-19) surveillance, in Brazil, initiated shortly after its description, in China. Our aim was to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and additional pathogens in samples from the initial phase ... ...

    Abstract Coronavirus disease 2019 (COVID-19) surveillance, in Brazil, initiated shortly after its description, in China. Our aim was to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and additional pathogens in samples from the initial phase of the outbreak in Brazil, from late February to late March. From 707 samples analysed, 29 (4.1%) were SARS-CoV-2 positive. Fever and cough were their most prevalent symptoms. Co-detection of rhinovirus was observed in 2 (6.9%) cases. Additional pathogens were identified in 66.1% of the SARS-CoV-2 negative cases, mainly rhinovirus and influenza A(H1N1)pdm09. Thus, we emphasise the importance of differential diagnosis in COVID-19 suspected cases.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #788945
    Database COVID19

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  4. Article ; Online: Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil.

    Pereira, Polyana Silva / Borghi, Mirla / Albano, Rodolpho Mattos / Lopes, Jonathan Christian Oliveira / Silveira, Melise Chaves / Marques, Elizabeth Andrade / Oliveira, Jane Cleide Ribeiro / Asensi, Marise Dutra / Carvalho-Assef, Ana Paula D'Alincourt

    Microbial drug resistance (Larchmont, N.Y.)

    2015  Volume 21, Issue 2, Page(s) 234–236

    Abstract: The most important resistance mechanism against β-lactam drugs is the production of carbapenemases. In this study, we report the first identification of Klebsiella pneumoniae carbapenemase (KPC)-2 and New Delhi metallo-β-lactamase (NDM)-1 in Enterobacter ...

    Abstract The most important resistance mechanism against β-lactam drugs is the production of carbapenemases. In this study, we report the first identification of Klebsiella pneumoniae carbapenemase (KPC)-2 and New Delhi metallo-β-lactamase (NDM)-1 in Enterobacter hormaechei subps. oharae from Brazil. The detection of carbapenemases was done by phenotypic assays, PCR, and DNA sequencing, whereas the identification was performed by conventional techniques, sequencing of the 16S rDNA gene, and hsp60-genotyping. Molecular typing was performed using pulsed-field gel electrophoresis, and antimicrobial susceptibility was surrogated by the Etest methodology. Using the whole genome sequencing approach, we searched for resistance genes, plasmid incompatibility group genes, and the genetic environment of blaNDM and blaKPC. The plasmid identification was done by restriction digests with the S1 nuclease followed by hybridization using digoxigenin labeled specific probes. The isolate was considered multiresistant, being susceptible to amikacin and polymyxin B. We observed the following resistance genes: blaCTX-M-15, blaACT-7, blaTEM-1, blaOXA-1, aadA1, aadA2, strA, strB, aac(3)-II, qnrB1, and aac(6')-Ib-cr and incompatibility group plasmid genes IncA/C, IncHI2, and IncN. The blaKPC gene was found associated to the transposon Tn4401 isoform b in plasmid with 50 kb (IncN) and blaNDM-1 was flanked by a truncated ISAba125 and bleMBL in plasmid with 160 kb (IncA/C). This study showed the coproduction of two important carbapenemases (KPC-2 and NDM-1) associated with mobile genetic elements of worldwide epidemiological importance (Tn4401 and ISAba125, respectively), reinforcing the idea that urgent measures are necessary to reduce and prevent the spreading of these carbapenemases primarily in the hospital settings.
    MeSH term(s) Adult ; Amikacin/therapeutic use ; Anti-Bacterial Agents/therapeutic use ; Bacterial Proteins/genetics ; Brazil ; DNA Transposable Elements/genetics ; DNA, Bacterial/genetics ; Drug Resistance, Multiple, Bacterial/drug effects ; Drug Resistance, Multiple, Bacterial/genetics ; Enterobacter/drug effects ; Enterobacter/genetics ; Female ; Genotype ; Humans ; Klebsiella Infections/drug therapy ; Klebsiella Infections/microbiology ; Klebsiella pneumoniae/drug effects ; Klebsiella pneumoniae/genetics ; Plasmids/genetics ; Polymyxin B/therapeutic use ; beta-Lactamases/genetics ; beta-Lactams/pharmacology
    Chemical Substances Anti-Bacterial Agents ; Bacterial Proteins ; DNA Transposable Elements ; DNA, Bacterial ; beta-Lactams ; Polymyxin B (1404-26-8) ; Amikacin (84319SGC3C) ; beta-lactamase KPC-2 (EC 3.5.2.-) ; beta-Lactamases (EC 3.5.2.6) ; beta-lactamase NDM-1 (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6)
    Language English
    Publishing date 2015-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1290490-9
    ISSN 1931-8448 ; 1076-6294
    ISSN (online) 1931-8448
    ISSN 1076-6294
    DOI 10.1089/mdr.2014.0171
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4435: Show issues Location:
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