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  1. Book: Immunocytochemistry and related techniques

    Merighi, Adalberto / Lossi, Laura

    (Neuromethods, ; 101 ; Springer protocols)

    2015  

    Author's details ed. by Adalberto Merighi and Laura Lossi
    Series title Neuromethods, ; 101
    Springer protocols
    Neuromethods
    Collection Neuromethods
    Keywords Immunohistochemistry / methods ; Histocytological Preparation Techniques / methods ; Cell Physiological Processes ; Chemistry Techniques, Analytical / methods
    Language English
    Size XIII, 473 S. : Ill., graph. Darst.
    Publisher Humana Press
    Publishing place New York u.a.
    Publishing country United States
    Document type Book
    Note Includes bibliographical references and index
    HBZ-ID HT018749798
    ISBN 978-1-4939-2312-0 ; 9781493923137 ; 1-4939-2312-9 ; 1493923137
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2015 A 3298 available for loan (reading room) Lesesaal EG

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  2. Book: Neuronal cell death

    Lossi, Laura / Merighi, Adalberto

    methods and protocols

    (Methods in molecular biology ; 1254 ; Springer protocols)

    2015  

    Author's details ed. by Laura Lossi and Adalberto Merighi
    Series title Methods in molecular biology ; 1254
    Springer protocols
    Collection
    Keywords Cell death ; Nervous system/Degeneration ; Neurons
    Subject code 571.936
    Language English
    Size XIV, 396 S. : Ill., graph. Darst., 27 cm
    Publisher Humana Press
    Publishing place New York u.a.
    Publishing country United States
    Document type Book
    Note Includes bibliographical references and index
    HBZ-ID HT018512202
    ISBN 978-1-4939-2151-5 ; 9781493921522 ; 1-4939-2151-7 ; 1493921525
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -1254- verfügbar in Königswinter Königswinter

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  3. Article ; Online: The concept of intrinsic versus extrinsic apoptosis.

    Lossi, Laura

    The Biochemical journal

    2022  Volume 479, Issue 3, Page(s) 357–384

    Abstract: Regulated cell death is a vital and dynamic process in multicellular organisms that maintains tissue homeostasis and eliminates potentially dangerous cells. Apoptosis, one of the better-known forms of regulated cell death, is activated when cell-surface ... ...

    Abstract Regulated cell death is a vital and dynamic process in multicellular organisms that maintains tissue homeostasis and eliminates potentially dangerous cells. Apoptosis, one of the better-known forms of regulated cell death, is activated when cell-surface death receptors like Fas are engaged by their ligands (the extrinsic pathway) or when BCL-2-family pro-apoptotic proteins cause the permeabilization of the mitochondrial outer membrane (the intrinsic pathway). Both the intrinsic and extrinsic pathways of apoptosis lead to the activation of a family of proteases, the caspases, which are responsible for the final cell demise in the so-called execution phase of apoptosis. In this review, I will first discuss the most common types of regulated cell death on a morphological basis. I will then consider in detail the molecular pathways of intrinsic and extrinsic apoptosis, discussing how they are activated in response to specific stimuli and are sometimes overlapping. In-depth knowledge of the cellular mechanisms of apoptosis is becoming more and more important not only in the field of cellular and molecular biology but also for its translational potential in several pathologies, including neurodegeneration and cancer.
    MeSH term(s) Animals ; Apoptosis/physiology ; Apoptosis Regulatory Proteins/physiology ; Apoptosomes/physiology ; Apoptosomes/ultrastructure ; Autophagy ; Caspases/physiology ; Humans ; Invertebrates/cytology ; Ligands ; Lysosomes/physiology ; Macrophages/physiology ; Mitochondrial Membranes/physiology ; Necrosis ; Neoplasm Proteins/physiology ; Permeability ; Phagocytosis ; Proto-Oncogene Proteins c-bcl-2/physiology ; Receptors, Death Domain/physiology
    Chemical Substances Apoptosis Regulatory Proteins ; Apoptosomes ; Ligands ; Neoplasm Proteins ; Proto-Oncogene Proteins c-bcl-2 ; Receptors, Death Domain ; Caspases (EC 3.4.22.-)
    Language English
    Publishing date 2022-02-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 2969-5
    ISSN 1470-8728 ; 0006-2936 ; 0306-3275 ; 0264-6021
    ISSN (online) 1470-8728
    ISSN 0006-2936 ; 0306-3275 ; 0264-6021
    DOI 10.1042/BCJ20210854
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.110: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Anatomical features for an adequate choice of the experimental animal model in biomedicine: III. Ferret, goat, sheep, and horse.

    Lossi, Laura

    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

    2022  Volume 244, Page(s) 151978

    Abstract: The anatomical characteristics of each of the many species today employed in biomedical research are very important when selecting the correct animal model(s), especially for conducting translational research. In previous papers, these features have been ...

    Abstract The anatomical characteristics of each of the many species today employed in biomedical research are very important when selecting the correct animal model(s), especially for conducting translational research. In previous papers, these features have been considered for fish (D'Angelo et al., 2016), the most common laboratory rodents, rabbits, and pigs (Lossi et al. 2016). I here follow this line of discussion by dealing with the importance of proper knowledge of ferrets, goats, sheep, and horses' main anatomical features in translational research.
    MeSH term(s) Sheep ; Horses ; Animals ; Rabbits ; Swine ; Goats ; Ferrets ; Models, Animal
    Language English
    Publishing date 2022-07-03
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 1106738-x
    ISSN 1618-0402 ; 0940-9602
    ISSN (online) 1618-0402
    ISSN 0940-9602
    DOI 10.1016/j.aanat.2022.151978
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ub I Zs.55: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Book ; Online: Reelin-Related Neurological Disorders and Animal Models

    DArcangelo, Gabriella / Merighi, Adalberto / Lossi, Laura

    2017  

    Abstract: The Reeler mutation was so named because of the alterations in gait that characterize homozygous mice. Several decades after the description of the Reeler phenotype, the mutated protein was discovered and named Reelin (Reln). Reln controls a number of ... ...

    Abstract The Reeler mutation was so named because of the alterations in gait that characterize homozygous mice. Several decades after the description of the Reeler phenotype, the mutated protein was discovered and named Reelin (Reln). Reln controls a number of fundamental steps in embryonic and postnatal brain development. A prominent embryonic function is the control of radial neuronal migration. As a consequence, homozygous Reeler mutants show disrupted cell layering in cortical brain structures. Reln also promotes postnatal neuronal maturation. Heterozygous mutants exhibit defects in dendrite extension and synapse formation, correlating with behavioral and cognitive deficits that are detectable at adult ages. The Reln-encoding gene is highly conserved between mice and humans. In humans, homozygous RELN mutations cause lissencephaly with cerebellar hypoplasia, a severe neuronal migration disorder that is reminiscent of the Reeler phenotype. In addition, RELN deficiency or dysfunction is also correlated with psychiatric and cognitive disorders, such as schizophrenia, bipolar disorder and autism, as well as some forms of epilepsy and Alzheimers disease. Despite the wealth of anatomical studies of the Reeler mouse brain, and the molecular dissection of Reln signaling mechanisms, the consequences of Reln deficiency on the development and function of the human brain are not yet completely understood. This Research Topic include reviews that summarize our current knowledge of the molecular aspects of Reln function, original articles that advance our understanding of its expression and function in different brain regions, and reviews that critically assess the potential role of Reln in human psychiatric and cognitive disorders
    Keywords Neurosciences. Biological psychiatry. Neuropsychiatry ; Science (General)
    Size 1 electronic resource (179 p.)
    Publisher Frontiers Media SA
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT020095033
    ISBN 9782889451111 ; 2889451119
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  6. Article ; Online: Co-cultures of cerebellar slices from mice with different

    Merighi, Adalberto / Lossi, Laura

    F1000Research

    2023  Volume 11, Page(s) 1183

    Abstract: Background: ...

    Abstract Background:
    MeSH term(s) Animals ; Mice ; Cell Adhesion Molecules, Neuronal/genetics ; Cell Adhesion Molecules, Neuronal/metabolism ; Cerebellum ; Coculture Techniques ; Extracellular Matrix Proteins/genetics ; Extracellular Matrix Proteins/metabolism ; Serine Endopeptidases/genetics ; Serine Endopeptidases/metabolism ; Reelin Protein/genetics
    Chemical Substances Cell Adhesion Molecules, Neuronal ; Extracellular Matrix Proteins ; Serine Endopeptidases (EC 3.4.21.-) ; Reln protein, mouse (EC 3.4.21.-) ; Reelin Protein
    Language English
    Publishing date 2023-10-02
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2699932-8
    ISSN 2046-1402 ; 2046-1402
    ISSN (online) 2046-1402
    ISSN 2046-1402
    DOI 10.12688/f1000research.126787.2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: An Overview of the Epigenetic Modifications in the Brain under Normal and Pathological Conditions.

    Lossi, Laura / Castagna, Claudia / Merighi, Adalberto

    International journal of molecular sciences

    2024  Volume 25, Issue 7

    Abstract: Epigenetic changes are changes in gene expression that do not involve alterations to the DNA sequence. These changes lead to establishing a so-called epigenetic code that dictates which and when genes are activated, thus orchestrating gene regulation and ...

    Abstract Epigenetic changes are changes in gene expression that do not involve alterations to the DNA sequence. These changes lead to establishing a so-called epigenetic code that dictates which and when genes are activated, thus orchestrating gene regulation and playing a central role in development, health, and disease. The brain, being mostly formed by cells that do not undergo a renewal process throughout life, is highly prone to the risk of alterations leading to neuronal death and neurodegenerative disorders, mainly at a late age. Here, we review the main epigenetic modifications that have been described in the brain, with particular attention on those related to the onset of developmental anomalies or neurodegenerative conditions and/or occurring in old age. DNA methylation and several types of histone modifications (acetylation, methylation, phosphorylation, ubiquitination, sumoylation, lactylation, and crotonylation) are major players in these processes. They are directly or indirectly involved in the onset of neurodegeneration in Alzheimer's or Parkinson's disease. Therefore, this review briefly describes the roles of these epigenetic changes in the mechanisms of brain development, maturation, and aging and some of the most important factors dynamically regulating or contributing to these changes, such as oxidative stress, inflammation, and mitochondrial dysfunction.
    MeSH term(s) Brain ; Epigenesis, Genetic ; DNA Methylation ; Protein Processing, Post-Translational ; Acetylation
    Language English
    Publishing date 2024-03-30
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25073881
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Endoplasmic Reticulum Stress Signaling and Neuronal Cell Death.

    Merighi, Adalberto / Lossi, Laura

    International journal of molecular sciences

    2022  Volume 23, Issue 23

    Abstract: Besides protein processing, the endoplasmic reticulum (ER) has several other functions such as lipid synthesis, the transfer of molecules to other cellular compartments, and the regulation of ... ...

    Abstract Besides protein processing, the endoplasmic reticulum (ER) has several other functions such as lipid synthesis, the transfer of molecules to other cellular compartments, and the regulation of Ca
    Language English
    Publishing date 2022-12-02
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms232315186
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Association of Caspase 3 Activation and H2AX γ Phosphorylation in the Aging Brain: Studies on Untreated and Irradiated Mice.

    Gionchiglia, Nadia / Granato, Alberto / Merighi, Adalberto / Lossi, Laura

    Biomedicines

    2021  Volume 9, Issue 9

    Abstract: Phosphorylation of H2AX is a response to DNA damage, but γH2AX also associates with mitosis and/or apoptosis. We examined the effects of X-rays on DNA integrity to shed more light on the significance of H2AX phosphorylation and its relationship with ... ...

    Abstract Phosphorylation of H2AX is a response to DNA damage, but γH2AX also associates with mitosis and/or apoptosis. We examined the effects of X-rays on DNA integrity to shed more light on the significance of H2AX phosphorylation and its relationship with activation of caspase 3 (CASP3), the main apoptotic effector. After administration of the S phase marker BrdU, brains were collected from untreated and irradiated (10 Gray) 24-month-old mice surviving 15 or 30 min after irradiation. After paraffin embedding, brain sections were single- or double-stained with antibodies against γH2AX, p53-binding protein 1 (53BP1) (which is recruited during the DNA damage response (DDR)), active CASP3 (cCASP3), 5-Bromo-2-deoxyuridine (BrdU), and phosphorylated histone H3 (pHH3) (which labels proliferating cells). After statistical analysis, we demonstrated that irradiation not only induced a robust DDR with the appearance of γH2AX and upregulation of 53BP1 but also that cells with damaged DNA attempted to synthesize new genetic material from the rise in BrdU immunostaining, with increased expression of cCASP3. Association of γH2AX, 53BP1, and cCASP3 was also evident in normal nonirradiated mice, where DNA synthesis appeared to be linked to disturbances in DNA repair mechanisms rather than true mitotic activity.
    Language English
    Publishing date 2021-09-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines9091166
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: The Phosphorylated Form of the Histone H2AX (γH2AX) in the Brain from Embryonic Life to Old Age.

    Merighi, Adalberto / Gionchiglia, Nadia / Granato, Alberto / Lossi, Laura

    Molecules (Basel, Switzerland)

    2021  Volume 26, Issue 23

    Abstract: The γ phosphorylated form of the histone H2AX (γH2AX) was described more than 40 years ago and it was demonstrated that phosphorylation of H2AX was one of the first cellular responses to DNA damage. Since then, γH2AX has been implicated in diverse ... ...

    Abstract The γ phosphorylated form of the histone H2AX (γH2AX) was described more than 40 years ago and it was demonstrated that phosphorylation of H2AX was one of the first cellular responses to DNA damage. Since then, γH2AX has been implicated in diverse cellular functions in normal and pathological cells. In the first part of this review, we will briefly describe the intervention of H2AX in the DNA damage response (DDR) and its role in some pivotal cellular events, such as regulation of cell cycle checkpoints, genomic instability, cell growth, mitosis, embryogenesis, and apoptosis. Then, in the main part of this contribution, we will discuss the involvement of γH2AX in the normal and pathological central nervous system, with particular attention to the differences in the DDR between immature and mature neurons, and to the significance of H2AX phosphorylation in neurogenesis and neuronal cell death. The emerging picture is that H2AX is a pleiotropic molecule with an array of yet not fully understood functions in the brain, from embryonic life to old age.
    MeSH term(s) Aging/metabolism ; Animals ; Brain/embryology ; Brain/metabolism ; Brain/pathology ; Embryo, Mammalian/metabolism ; Histones/metabolism ; Humans ; Oxidative Stress ; Phosphorylation
    Chemical Substances Histones
    Language English
    Publishing date 2021-11-27
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules26237198
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.MO 81: Show issues

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