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  1. Article: Structure and function of a chloroplast DNA replication origin of Chlamydomonas reinhardtii.

    Wu, M / Lou, J K / Chang, D Y / Chang, C H / Nie, Z Q

    Proceedings of the National Academy of Sciences of the United States of America

    2002  Volume 83, Issue 18, Page(s) 6761–6765

    Abstract: Chloroplast DNA replication in Chlamydomonas reinhardtii is initiated by the formation of a displacement loop (D-loop) at a specific site. One D-loop site with its flanking sequence was cloned in recombinant plasmids SC3-1 and R-13. The sequence of the ... ...

    Abstract Chloroplast DNA replication in Chlamydomonas reinhardtii is initiated by the formation of a displacement loop (D-loop) at a specific site. One D-loop site with its flanking sequence was cloned in recombinant plasmids SC3-1 and R-13. The sequence of the chloroplast DNA insert in SC3-1, which includes the 0.42-kilobase (kb) D-loop region, as well as 0.2 kb to the 5' end and 0.43 kb to the 3' end of the D-loop region, was determined. The sequence is A+T-rich and contains four large stem-loop stuctures. An open reading frame potentially coding for a polypeptide of 136 amino acids was detected in the D-loop region. One stem-loop structure and two back-to-back prokaryotic-type promoters were mapped within the open reading frame. The 5.5-kb EcoRI fragment cloned in R-13 contains the 1.05-kb SC3-1 insert and its flanking regions. A yeast autonomously replicating (ARS) sequence and an ARC sequence, which promotes autonomous replication in Chlamydomonas, have been mapped within the flanking regions [Vallet, J.-M. & Rochaix, J.-D. (1985) Curr. Genet. 9, 321-324]. Both R-13 and SC3-1 were active as templates in a crude algal preparation that supports DNA synthesis. In this in vitro system, chloroplast DNA synthesis initiated near the D-loop site.
    Language English
    Publishing date 2002-09-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.83.18.6761
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1148: Show issues Location:
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  2. Article: Nucleotide sequence of the chloroplast ribosomal protein gene L14 in Chlamydomonas reinhardtii.

    Lou, J K / Cruz, F D / Wu, M

    Nucleic acids research

    1989  Volume 17, Issue 9, Page(s) 3587

    MeSH term(s) Amino Acid Sequence ; Base Sequence ; Chlamydomonas/genetics ; Chloroplasts/metabolism ; Genes ; Molecular Sequence Data ; Ribosomal Proteins/genetics
    Chemical Substances Ribosomal Proteins ; ribosomal protein L14
    Language English
    Publishing date 1989-05-11
    Publishing country England
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 186809-3
    ISSN 0305-1048 ; 0301-5610
    ISSN 0305-1048 ; 0301-5610
    DOI 10.1093/nar/17.9.3587
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1067: Show issues Location:
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  3. Article: Long-lasting decrease in neuronal Ca2+/calmodulin-dependent protein kinase II activity in a hippocampal neuronal culture model of spontaneous recurrent seizures.

    Blair, R E / Churn, S B / Sombati, S / Lou, J K / DeLorenzo, R J

    Brain research

    1999  Volume 851, Issue 1-2, Page(s) 54–65

    Abstract: Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) activity was evaluated in a well-characterized in vitro model of epileptiform activity. Long-lasting spontaneous recurrent seizure (SRS) activity was induced in hippocampal neuronal cultures by ... ...

    Abstract Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) activity was evaluated in a well-characterized in vitro model of epileptiform activity. Long-lasting spontaneous recurrent seizure (SRS) activity was induced in hippocampal neuronal cultures by exposure to low Mg2+ media for 3 h. Analysis of endogenous Ca2+/calmodulin-dependent phosphorylation revealed a significant long-lasting decrease in 32P incorporation into the alpha (50 kDa) and beta (60 kDa) subunits of CaM kinase II in association with the induction of SRS activity in this preparation. Ca2+/calmodulin-dependent substrate phosphorylation of the synthetic peptides, Autocamtide-2 and Syntide II, was also significantly reduced following the induction of SRSs and persisted for the life of the neurons in culture. The decrement in CaM kinase II activity associated with low Mg2+ treatment remained significantly decreased when values were corrected for changes in levels of alpha subunit immunoreactivity and neuronal cell loss. Addition of the protein phosphatase inhibitors, okadaic acid and cyclosporin A, to the phosphorylation reaction did not block the SRS-associated decrease in substrate phosphorylation, indicating that enhanced phosphatase activity was not a contributing factor to the observed decrease in phosphate incorporation. The findings of this study demonstrate that CaM kinase II activity is decreased in association with epileptogenesis observed in these hippocampal cultures and may contribute to the production and maintenance of SRSs in this model.
    MeSH term(s) Animals ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 ; Calcium-Calmodulin-Dependent Protein Kinases/metabolism ; Cells, Cultured ; Hippocampus/metabolism ; Magnesium Chloride ; Neurons/cytology ; Neurons/metabolism ; Rats ; Rats, Sprague-Dawley ; Recurrence ; Seizures/chemically induced ; Seizures/metabolism ; Synaptic Transmission/physiology
    Chemical Substances Magnesium Chloride (02F3473H9O) ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 (EC 2.7.11.17) ; Calcium-Calmodulin-Dependent Protein Kinases (EC 2.7.11.17)
    Language English
    Publishing date 1999-12-18
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 1200-2
    ISSN 1872-6240 ; 0006-8993
    ISSN (online) 1872-6240
    ISSN 0006-8993
    DOI 10.1016/s0006-8993(99)02100-9
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 161: Show issues Location:
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  4. Article: Localization of a r-protein gene within the chloroplast DNA replication origin of Chlamydomonas.

    Lou, J K / Wu, M / Chang, C H / Cuticchia, A J

    Current genetics

    1987  Volume 11, Issue 6-7, Page(s) 537–541

    Abstract: In our previous study of chloroplast (Cp) DNA replication in Chlamydomonas reinhardtii, one D-loop site with its flanking regions was cloned and sequenced. The D-loop site mapped by electron microscopy (EM) overlaps with an open reading frame (ORF) ... ...

    Abstract In our previous study of chloroplast (Cp) DNA replication in Chlamydomonas reinhardtii, one D-loop site with its flanking regions was cloned and sequenced. The D-loop site mapped by electron microscopy (EM) overlaps with an open reading frame (ORF) potentially coding for a polypeptide of 136 amino acids. In this report, the corresponding D-loop isolated from another species of Chlamydomonas was sequenced. An ORF was also detected. Sequence comparison indicated that most conserved sequences between these two cloned origins are located within the ORF. Amino acid sequences of these two ORFs are highly conserved. The corresponding sequence for this ORF in the tobacco Cp genome was located by a Southern blotting analysis. Since the complete sequence data of Cp DNAs from a liverwort and from tobacco have been determined in 2 Japanese laboratories recently, it has been possible for us to show that this ORF encodes a protein homologous to the Cp ribosomal protein (r-protein) L16, by sequence comparison.
    MeSH term(s) Amino Acid Sequence ; Base Sequence ; Chlamydomonas/genetics ; Chloroplasts/metabolism ; DNA Replication ; Genes ; Molecular Sequence Data ; Nuclear Proteins/genetics ; Sequence Homology, Nucleic Acid
    Chemical Substances Nuclear Proteins
    Language English
    Publishing date 1987
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 282876-5
    ISSN 1432-0983 ; 0172-8083
    ISSN (online) 1432-0983
    ISSN 0172-8083
    DOI 10.1007/bf00384617
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2586: Show issues Location:
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  5. Article: Localization of a r-protein gene with the chloroplast DNA replication origin of Chlamydomonas

    Lou, J.K / Wu, M / Chang, C.H / Cuticchia, A.J

    Current genetics. 1987. . v. 11 (6/7)

    1987  

    Keywords Chlamydomonas ; chloroplasts ; DNA ; genes ; nucleotide sequences
    Language English
    Size p. 537-541., ill.
    Document type Article
    ZDB-ID 282876-5
    ISSN 1432-0983 ; 0172-8083
    ISSN (online) 1432-0983
    ISSN 0172-8083
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 81/707: Show issues

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