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  1. Article ; Online: Chiropterans as a potential hosts of Leishmania spp. in endemic areas for leishmaniasis in northeastern Brazil.

    Ramos, Luis Gustavo Siqueira Matias / Costa, Valéria Almeida / Louzeiro, Nayara Mendes / Carvalho, Clauberth César Alves / de Maria Seabra Nogueira, Rita / Speranca, Marcia Aparecida / Cabral, Aline Diniz / Costa, Francisco Borges / Barros, Maria Claudene / da Costa Fraga, Elmary / Marcili, Arlei / da Costa, Andréa Pereira

    Zoonoses and public health

    2022  Volume 69, Issue 8, Page(s) 987–992

    Abstract: The species of the genus Leishmania are protozoa that are widely distributed from Asia to the Americas, affecting humans and wild and domestic animals. Little is known about infection by Leishmania in bats in the state of Maranhão, Brazil. The objective ... ...

    Abstract The species of the genus Leishmania are protozoa that are widely distributed from Asia to the Americas, affecting humans and wild and domestic animals. Little is known about infection by Leishmania in bats in the state of Maranhão, Brazil. The objective of this study was to investigate the presence of Leishmania in bats in Maranhão. Blood samples were collected from bat species for parasitological diagnosis. Samples of spleen and liver were collected for molecular analysis. All the blood cultures were negative. In two blood smears, organisms similar to amastigotes of Leishmania sp. were detected. Of the 116 samples, two spleen samples were positive and showed similarity to Leishmania infantum. Therefore, further studies are needed to elucidate whether bats take part in the epidemiological chain of leishmaniasis.
    MeSH term(s) Humans ; Animals ; Leishmaniasis, Visceral/epidemiology ; Leishmaniasis, Visceral/veterinary ; Leishmaniasis, Visceral/parasitology ; Chiroptera ; Brazil/epidemiology ; Leishmaniasis/epidemiology ; Leishmaniasis/veterinary ; Leishmania infantum
    Language English
    Publishing date 2022-08-26
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2271118-1
    ISSN 1863-2378 ; 1863-1959
    ISSN (online) 1863-2378
    ISSN 1863-1959
    DOI 10.1111/zph.12997
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Chiropterans as a potential hosts of Leishmania spp. in endemic areas for leishmaniasis in northeastern Brazil

    Ramos, Luis Gustavo Siqueira Matias / Costa, Valéria Almeida / Louzeiro, Nayara Mendes / Carvalho, Clauberth César Alves / de Maria Seabra Nogueira, Rita / Speranca, Marcia Aparecida / Cabral, Aline Diniz / Costa, Francisco Borges / Barros, Maria Claudene / da Costa Fraga, Elmary / Marcili, Arlei / da Costa, Andréa Pereira

    Zoonoses and Public Health. 2022 Dec., v. 69, no. 8 p.987-992

    2022  

    Abstract: The species of the genus Leishmania are protozoa that are widely distributed from Asia to the Americas, affecting humans and wild and domestic animals. Little is known about infection by Leishmania in bats in the state of Maranhão, Brazil. The objective ... ...

    Abstract The species of the genus Leishmania are protozoa that are widely distributed from Asia to the Americas, affecting humans and wild and domestic animals. Little is known about infection by Leishmania in bats in the state of Maranhão, Brazil. The objective of this study was to investigate the presence of Leishmania in bats in Maranhão. Blood samples were collected from bat species for parasitological diagnosis. Samples of spleen and liver were collected for molecular analysis. All the blood cultures were negative. In two blood smears, organisms similar to amastigotes of Leishmania sp. were detected. Of the 116 samples, two spleen samples were positive and showed similarity to Leishmania infantum. Therefore, further studies are needed to elucidate whether bats take part in the epidemiological chain of leishmaniasis.
    Keywords Chiroptera ; Leishmania infantum ; amastigotes ; blood ; leishmaniasis ; liver ; public health ; spleen ; zoonoses ; Asia ; Brazil
    Language English
    Dates of publication 2022-12
    Size p. 987-992.
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 2271118-1
    ISSN 1863-2378 ; 1863-1959
    ISSN (online) 1863-2378
    ISSN 1863-1959
    DOI 10.1111/zph.12997
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Diagnosis and epidemiology of Leishmania infantum in domestic cats in an endemic area of the Amazon region, Brazil.

    Rocha, Ana Vitória Verde Oliveira / Moreno, Brenda Fernanda Sodré / Cabral, Aline Diniz / Louzeiro, Nayara Mendes / Miranda, Leandro Macedo / Santos, Vivian Magalhães Brandão Dos / Costa, Francisco Borges / Nogueira, Rita de Maria Seabra / Marcili, Arlei / Sperança, Márcia Aparecida / Costa, Andréa Pereira da

    Veterinary parasitology

    2019  Volume 273, Page(s) 80–85

    Abstract: Visceral leishmaniasis is a zoonotic disease caused by Leishmania infantum for which dogs are the main reservoir. In South America, presence of this disease is expanding along with increasing dispersion of its principal vector, the sand-fly Lutzomyia ... ...

    Abstract Visceral leishmaniasis is a zoonotic disease caused by Leishmania infantum for which dogs are the main reservoir. In South America, presence of this disease is expanding along with increasing dispersion of its principal vector, the sand-fly Lutzomyia longipalpis. Feline leishmaniasis is an emerging disease in domestic cats, but epidemiological studies in endemic areas of the Amazon region of Brazil are scarce and the role of cats as reservoirs of L. infantum has been debated. The aim of this study was to investigate L. infantum infection in cats living in the Amazon biome region, using serological and molecular methods. A total of 105 cats were subjected to clinical examination and blood samples were taken for immunofluorescent-antibody (IFAT) serological evaluation, to determine anti-Leishmania antibody titers. Conventional PCR and Sanger's sequencing targeting L. infantum chitinase and Leishmania species ribosomal internal transcribed spacer (ITS-1) encoding genes were performed on conjunctival swabs from these cats. Seropositivity was detected in 32 animals (30.48%), thus confirming that contact between these cats and the parasite was occurring. PCR followed by amplicon sequencing showed that three samples (2.86%) were positive for a chitinase gene and six (5.71%) were positive for the ITS-1 gene. Parasite-positive diagnoses presented a statistically significant association with free access to the streets (p = 0.0111), cohabitation with dogs affected previously by VL (p = 0.0006) and absence of backyard cleaning and garbage collection (p = 0.00003). These results emphasize that cats should be included in epidemiological surveys of leishmaniasis, especially in endemic areas, if not as the reservoir host (unproven), at least as a "sentinel host" that is useful for revealing situations of endemic circulation of L. infantum. Moreover, in these areas, feline leishmaniasis needs to be considered in the differential diagnosis among domestic cats presenting alopecia, rarefied hair, lacerations and ulcerative dermatitis.
    MeSH term(s) Animals ; Antibodies, Protozoan/blood ; Brazil/epidemiology ; Cat Diseases/diagnosis ; Cat Diseases/epidemiology ; Cat Diseases/parasitology ; Cats ; DNA, Ribosomal Spacer/genetics ; Dogs ; Fluorescent Antibody Technique, Indirect/veterinary ; Leishmania infantum ; Leishmaniasis, Visceral/diagnosis ; Leishmaniasis, Visceral/epidemiology ; Leishmaniasis, Visceral/veterinary ; Polymerase Chain Reaction ; Prevalence ; Risk Factors
    Chemical Substances Antibodies, Protozoan ; DNA, Ribosomal Spacer
    Language English
    Publishing date 2019-08-15
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 196831-2
    ISSN 1873-2550 ; 0304-4017
    ISSN (online) 1873-2550
    ISSN 0304-4017
    DOI 10.1016/j.vetpar.2019.08.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4380: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Diagnosis and epidemiology of Leishmania infantum in domestic cats in an endemic area of the Amazon region, Brazil

    Rocha, Ana Vitória Verde Oliveira / Moreno, Brenda Fernanda Sodré / Cabral, Aline Diniz / Louzeiro, Nayara Mendes / Miranda, Leandro Macedo / Santos, Vivian Magalhães Brandão dos / Costa, Francisco Borges / Nogueira, Rita de Maria Seabra / Marcili, Arlei / Sperança, Márcia Aparecida / Costa, Andréa Pereira da

    Veterinary parasitology. 2019 Sept., v. 273

    2019  

    Abstract: Visceral leishmaniasis is a zoonotic disease caused by Leishmania infantum for which dogs are the main reservoir. In South America, presence of this disease is expanding along with increasing dispersion of its principal vector, the sand-fly Lutzomyia ... ...

    Abstract Visceral leishmaniasis is a zoonotic disease caused by Leishmania infantum for which dogs are the main reservoir. In South America, presence of this disease is expanding along with increasing dispersion of its principal vector, the sand-fly Lutzomyia longipalpis. Feline leishmaniasis is an emerging disease in domestic cats, but epidemiological studies in endemic areas of the Amazon region of Brazil are scarce and the role of cats as reservoirs of L. infantum has been debated. The aim of this study was to investigate L. infantum infection in cats living in the Amazon biome region, using serological and molecular methods. A total of 105 cats were subjected to clinical examination and blood samples were taken for immunofluorescent-antibody (IFAT) serological evaluation, to determine anti-Leishmania antibody titers. Conventional PCR and Sanger’s sequencing targeting L. infantum chitinase and Leishmania species ribosomal internal transcribed spacer (ITS-1) encoding genes were performed on conjunctival swabs from these cats. Seropositivity was detected in 32 animals (30.48%), thus confirming that contact between these cats and the parasite was occurring. PCR followed by amplicon sequencing showed that three samples (2.86%) were positive for a chitinase gene and six (5.71%) were positive for the ITS-1 gene. Parasite-positive diagnoses presented a statistically significant association with free access to the streets (p = 0.0111), cohabitation with dogs affected previously by VL (p = 0.0006) and absence of backyard cleaning and garbage collection (p = 0.00003). These results emphasize that cats should be included in epidemiological surveys of leishmaniasis, especially in endemic areas, if not as the reservoir host (unproven), at least as a "sentinel host" that is useful for revealing situations of endemic circulation of L. infantum. Moreover, in these areas, feline leishmaniasis needs to be considered in the differential diagnosis among domestic cats presenting alopecia, rarefied hair, lacerations and ulcerative dermatitis.
    Keywords Leishmania infantum ; Lutzomyia longipalpis ; alopecia ; antibodies ; blood sampling ; cat diseases ; cats ; chitinase ; cleaning ; clinical examination ; dermatitis ; dogs ; ecosystems ; emerging diseases ; epidemiological studies ; genes ; internal transcribed spacers ; lacerations ; municipal solid waste ; parasites ; polymerase chain reaction ; seroprevalence ; visceral leishmaniasis ; zoonoses ; Amazonia ; Brazil
    Language English
    Dates of publication 2019-09
    Size p. 80-85.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 196831-2
    ISSN 1873-2550 ; 0304-4017
    ISSN (online) 1873-2550
    ISSN 0304-4017
    DOI 10.1016/j.vetpar.2019.08.007
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4380: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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