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  1. Article ; Online: Epitope mapping of African swine fever virus (ASFV) structural protein, p54.

    Petrovan, Vlad / Murgia, Maria V / Wu, Ping / Lowe, Andre D / Jia, Wei / Rowland, Raymond R R

    Virus research

    2020  Volume 279, Page(s) 197871

    Abstract: In the absence of a vaccine for African swine fever virus (ASFV), diagnostic tools are critical for early detection and implementation of control measures. Along with other immunogenic proteins, p54 is a good serological target for conducting ASF ... ...

    Abstract In the absence of a vaccine for African swine fever virus (ASFV), diagnostic tools are critical for early detection and implementation of control measures. Along with other immunogenic proteins, p54 is a good serological target for conducting ASF detection and surveillance. In this study, a panel of 12 mouse monoclonal antibodies (mAbs) was prepared against a baculovirus-expressed p54(60-178) polypeptide. Further screening showed that five mAbs were positive for reactivity against ASFV-infected cells and recombinant p54 proteins. Mapping studies using five polypeptides and 12 oligopeptides, showed that mAb #154-1 recognized a conserved polypeptide sequence, p54(65-75), and was placed into Group 1. Mabs #143-1 and #7 recognized a region covered by p54(93-113) and were placed into Group 2. Group 3 consisted of mAbs #101 and #117, which recognized p54(118-127). Sera from pigs infected with the low virulent OURT 88/3 strain recognized the same p54 region covered by the Group 3 mAbs. When tested in a neutralization format, only mAb #143-1 showed neutralization activity above background. Together, the results identify important antigenic and immunogenic regions located on p54, which provide new tools for improving ASFV diagnostics.
    MeSH term(s) African Swine Fever/virology ; African Swine Fever Virus/chemistry ; African Swine Fever Virus/genetics ; African Swine Fever Virus/immunology ; Animals ; Antibodies, Monoclonal/biosynthesis ; Antibodies, Monoclonal/immunology ; Antibodies, Viral/blood ; Antigens, Viral/immunology ; Baculoviridae/genetics ; Baculoviridae/immunology ; Chlorocebus aethiops ; Epitope Mapping/methods ; Mice ; Swine ; Vero Cells ; Viral Structural Proteins/genetics ; Viral Structural Proteins/immunology
    Chemical Substances Antibodies, Monoclonal ; Antibodies, Viral ; Antigens, Viral ; Viral Structural Proteins ; p54 protein, African swine fever virus
    Language English
    Publishing date 2020-01-28
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2020.197871
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1965: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Epitope mapping of African swine fever virus (ASFV) structural protein, p54

    Petrovan, Vlad / Murgia, Maria V / Wu, Ping / Lowe, Andre D / Jia, Wei / Rowland, Raymond R.R

    Virus research. 2020 Apr. 02, v. 279

    2020  

    Abstract: In the absence of a vaccine for African swine fever virus (ASFV), diagnostic tools are critical for early detection and implementation of control measures. Along with other immunogenic proteins, p54 is a good serological target for conducting ASF ... ...

    Abstract In the absence of a vaccine for African swine fever virus (ASFV), diagnostic tools are critical for early detection and implementation of control measures. Along with other immunogenic proteins, p54 is a good serological target for conducting ASF detection and surveillance. In this study, a panel of 12 mouse monoclonal antibodies (mAbs) was prepared against a baculovirus-expressed p54(60–178) polypeptide. Further screening showed that five mAbs were positive for reactivity against ASFV-infected cells and recombinant p54 proteins. Mapping studies using five polypeptides and 12 oligopeptides, showed that mAb #154-1 recognized a conserved polypeptide sequence, p54(65–75), and was placed into Group 1. Mabs #143-1 and #7 recognized a region covered by p54(93–113) and were placed into Group 2. Group 3 consisted of mAbs #101 and #117, which recognized p54(118–127). Sera from pigs infected with the low virulent OURT 88/3 strain recognized the same p54 region covered by the Group 3 mAbs. When tested in a neutralization format, only mAb #143-1 showed neutralization activity above background. Together, the results identify important antigenic and immunogenic regions located on p54, which provide new tools for improving ASFV diagnostics.
    Keywords African swine fever virus ; control methods ; diagnostic techniques ; epitope mapping ; mice ; monitoring ; monoclonal antibodies ; neutralization ; oligopeptides ; polypeptides ; screening ; structural proteins ; swine ; vaccines ; virulence
    Language English
    Dates of publication 2020-0402
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2020.197871
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1965: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Antigenic regions of African swine fever virus phosphoprotein P30.

    Wu, Ping / Lowe, Andre D / Rodríguez, Yelitza Y / Murgia, Maria V / Dodd, Kimberly A / Rowland, Raymond R / Jia, Wei

    Transboundary and emerging diseases

    2020  Volume 67, Issue 5, Page(s) 1942–1953

    Abstract: African swine fever (ASF) is one of the most complex and lethally haemorrhagic viral diseases of swine, affecting all breeds and ages of pigs. In the absence of ASF vaccines, reliable laboratory diagnosis and restricted biosecurity are critical for ... ...

    Abstract African swine fever (ASF) is one of the most complex and lethally haemorrhagic viral diseases of swine, affecting all breeds and ages of pigs. In the absence of ASF vaccines, reliable laboratory diagnosis and restricted biosecurity are critical for disease prevention and control. A detection of ASF-specific antibodies in an unvaccinated pig is a good marker for the diagnosis of ASF. The immunoperoxidase test (IPT) is a sensitive test for detecting ASF virus (ASFV) antibodies. However, due to the complexity of the procedure, the IPT is only suitable to be used as a confirmatory test. The ASFV p30 protein-based enzyme-linked immunosorbent assay (ELISA) is widely used for ASFV antibody screening, but the sensitivity is not comparable to the IPT. It is essential to have a better understanding of the antigenic properties of ASFV p30 to improve p30-based serologic tests. In this study, we developed a panel of 21 monoclonal antibodies (mAbs) against ASFV p30. With 14 out of the 21 mAbs, we defined 4 antigenic regions that contain at least 4 linear epitopes. Nine of the 14 mAbs mapped to antigenic regions 3 and 4 reacted with p30 in all serologic methods tested in this study, such as indirect immunofluorescence assay (IFA), ELISA and Western blot. The antigenic regions 3 and 4 are highly conserved and immunodominant in host antibody response. These mAbs and the defined p30 antigenic regions 3 and 4 provide valuable tools for the development and improvement of ASF serologic assays.
    Language English
    Publishing date 2020-03-20
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2414822-2
    ISSN 1865-1682 ; 1865-1674
    ISSN (online) 1865-1682
    ISSN 1865-1674
    DOI 10.1111/tbed.13533
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Linear epitopes in African swine fever virus p72 recognized by monoclonal antibodies prepared against baculovirus-expressed antigen.

    Heimerman, Mallory E / Murgia, Maria V / Wu, Ping / Lowe, Andre D / Jia, Wei / Rowland, Raymond R

    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

    2018  Volume 30, Issue 3, Page(s) 406–412

    Abstract: Protein p72 is the major capsid protein of African swine fever virus (ASFV) and is an important target for test and vaccine development. Monoclonal antibodies (mAbs) were prepared against a recombinant antigenic fragment, from amino acid (aa) 20-303, ... ...

    Abstract Protein p72 is the major capsid protein of African swine fever virus (ASFV) and is an important target for test and vaccine development. Monoclonal antibodies (mAbs) were prepared against a recombinant antigenic fragment, from amino acid (aa) 20-303, expressed in baculovirus. A total of 29 mAbs were recovered and tested by immunofluorescent antibody (IFA) staining on ASFV Lisbon-infected Vero cells. Six antibodies were IFA-positive and selected for further characterization. Epitope mapping was performed against overlapping polypeptides expressed in E. coli and oligopeptides. Based on oligopeptide recognition, the mAbs were divided into 4 groups: mAb 85 (aa 165-171); mAbs 65-3 and 6H9-1 (aa 265-280); mAbs 8F7-3 and 23 (aa 280-294); and mAb 4A4 (aa 290-303). All mAbs were located within a highly conserved region in p72. This panel of antibodies provides the opportunity to develop new assays for the detection of ASFV antibody and antigen.
    MeSH term(s) African Swine Fever/virology ; African Swine Fever Virus/immunology ; Animals ; Antibodies, Monoclonal/immunology ; Antibodies, Viral/immunology ; Antigens, Viral ; Capsid Proteins ; Enzyme-Linked Immunosorbent Assay/veterinary ; Epitopes ; Swine
    Chemical Substances Antibodies, Monoclonal ; Antibodies, Viral ; Antigens, Viral ; Capsid Proteins ; Epitopes ; capsid protein p72, African swine fever virus
    Language English
    Publishing date 2018-01-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 287603-6
    ISSN 1943-4936 ; 1040-6387
    ISSN (online) 1943-4936
    ISSN 1040-6387
    DOI 10.1177/1040638717753966
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3645: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Linear epitopes in African swine fever virus p72 recognized by monoclonal antibodies prepared against baculovirus-expressed antigen

    Heimerman, Mallory E. / Murgia, Maria V. / Wu, Ping / Lowe, Andre D. / Jia, Wei / Rowland, Raymond R.

    Journal of Veterinary Diagnostic Investigation. 2018 May, v. 30, no. 3 p.406-412

    2018  

    Abstract: Protein p72 is the major capsid protein of African swine fever virus (ASFV) and is an important target for test and vaccine development. Monoclonal antibodies (mAbs) were prepared against a recombinant antigenic fragment, from amino acid (aa) 20–303, ... ...

    Abstract Protein p72 is the major capsid protein of African swine fever virus (ASFV) and is an important target for test and vaccine development. Monoclonal antibodies (mAbs) were prepared against a recombinant antigenic fragment, from amino acid (aa) 20–303, expressed in baculovirus. A total of 29 mAbs were recovered and tested by immunofluorescent antibody (IFA) staining on ASFV Lisbon-infected Vero cells. Six antibodies were IFA-positive and selected for further characterization. Epitope mapping was performed against overlapping polypeptides expressed in E. coli and oligopeptides. Based on oligopeptide recognition, the mAbs were divided into 4 groups: mAb 85 (aa 165–171); mAbs 65-3 and 6H9-1 (aa 265–280); mAbs 8F7-3 and 23 (aa 280–294); and mAb 4A4 (aa 290–303). All mAbs were located within a highly conserved region in p72. This panel of antibodies provides the opportunity to develop new assays for the detection of ASFV antibody and antigen.
    Keywords African swine fever virus ; Escherichia coli ; amino acids ; antibodies ; coat proteins ; epitopes ; oligopeptides ; polypeptides ; vaccine development ; monoclonal antibodies ; p72
    Language English
    Dates of publication 2018-05
    Size p. 406-412.
    Publishing place SAGE Publications
    Document type Article ; Online
    Note Resource is Open Access
    ZDB-ID 287603-6
    ISSN 1943-4936 ; 1040-6387
    ISSN (online) 1943-4936
    ISSN 1040-6387
    DOI 10.1177/1040638717753966
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3645: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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