LIVIVO - Search results -

Search results

Result 1 - 10 of total 73

Search options

Article ; Online: Clinical and molecular epidemiology of enterovirus D68 from 2013 to 2020 in Shanghai.

Li, Fei / Lu, Rou-Jian / Zhang, Yu-Han / Shi, Peng / Ao, Yuan-Yun / Cao, Lin-Feng / Zhang, Yu-Lan / Tan, Wen-Jie / Shen, Jun

Scientific reports

2024 Volume 14, Issue 1, Page(s) 2161

Abstract: Enterovirus D68 (EV-D68) is an emerging pathogen that has caused outbreaks of severe respiratory disease worldwide, especially in children. We aim to investigate the prevalence and genetic characteristics of EV-D68 in children from Shanghai. ... ...

Abstract	Enterovirus D68 (EV-D68) is an emerging pathogen that has caused outbreaks of severe respiratory disease worldwide, especially in children. We aim to investigate the prevalence and genetic characteristics of EV-D68 in children from Shanghai. Nasopharyngeal swab or bronchoalveolar lavage fluid samples collected from children hospitalized with community-acquired pneumonia were screened for EV-D68. Nine of 3997 samples were EV-D68-positive. Seven of nine positive samples were sequenced and submitted to GenBank. Based on partial polyprotein gene (3D) or complete sequence analysis, we found the seven strains belong to different clades and subclades, including three D1 (detected in 2013 and 2014), one D2 (2013), one D3 (2019), and two B3 (2014 and 2018). Overall, we show different clades and subclades of EV-D68 spread with low positive rates (0.2%) among children in Shanghai between 2013 and 2020. Amino acid mutations were found in the epitopes of the VP1 BC and DE loops and C-terminus; similarity analysis provided evidence for recombination as an important mechanism of genomic diversification. Both single nucleotide mutations and recombination play a role in evolution of EV-D68. Genetic instability within these clinical strains may indicate large outbreaks could occur following cumulative mutations.
MeSH term(s)	Child ; Humans ; Molecular Epidemiology ; Enterovirus D, Human/genetics ; Respiratory Tract Infections/epidemiology ; Enterovirus Infections/epidemiology ; Phylogeny ; China/epidemiology ; Disease Outbreaks ; Enterovirus/genetics
Language	English
Publishing date	2024-01-25
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-52226-w
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Real-time reverse transcription-polymerase chain reaction assay panel for the detection of severe acute respiratory syndrome coronavirus 2 and its variants.

Lu, Rou-Jian / Zhao, Li / Huang, Bao-Ying / Ye, Fei / Wang, Wen-Ling / Tan, Wen-Jie

Chinese medical journal

2021 Volume 134, Issue 17, Page(s) 2048–2053

Abstract: Background: With the ongoing worldwide coronavirus disease 2019 (COVID-19) pandemic, an increasing number of viral variants are being identified, which poses a challenge for nucleic acid-based diagnostic tests. Rapid tests, such as real-time reverse ... ...

Abstract	Background: With the ongoing worldwide coronavirus disease 2019 (COVID-19) pandemic, an increasing number of viral variants are being identified, which poses a challenge for nucleic acid-based diagnostic tests. Rapid tests, such as real-time reverse transcription-polymerase chain reaction (rRT-PCR), play an important role in monitoring COVID-19 infection and controlling its spread. However, the changes in the genotypes of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may result in decreased sensitivity of the rRT-PCR assay and it is necessary to monitor the mutations in primers and probes of SARS-CoV-2 detection over time. Methods: We developed two rRT-PCR assays to detect the RNA-dependent RNA polymerase (RdRp) and nucleocapsid (N) genes of SARS-CoV-2. We evaluated these assays together with our previously published assays targeting the ORF1ab and N genes for the detection and confirmation of SARS-CoV-2 and its variants of concern (VOCs). In addition, we also developed two rRT-PCR assays (S484K and S501Y) targeting the spike gene, which when combined with the open reading frames (ORF)1ab assay, respectively, to form duplex rRT-PCR assays, were able to detect SARS-CoV-2 VOCs (lineages B.1.351 and B.1.1.7). Results: Using a SARS-CoV-2 stock with predetermined genomic copies as a standard, the detection limit of both assays targeting RdRp and N was five copies/reaction. Furthermore, no cross-reactions with six others human CoVs (229E, OC43, NL63, HKU1, severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus) were observed using these assays. In addition, the S484K and S501Y assays were combined with the ORF1ab assay, respectively. Conclusions: Four rRT-PCR assays (RdRp, N, S484K, and S501Y) were used to detect SARS-CoV-2 variants, and these assays were shown to be effective in screening for multiple virus strains.
MeSH term(s)	COVID-19 ; Humans ; RNA, Viral/genetics ; Real-Time Polymerase Chain Reaction ; Reverse Transcription ; SARS-CoV-2 ; Sensitivity and Specificity
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-08-16
Publishing country	China
Document type	Journal Article
ZDB-ID	127089-8
ISSN	2542-5641 ; 0366-6999 ; 1002-0187
ISSN (online)	2542-5641
ISSN	0366-6999 ; 1002-0187
DOI	10.1097/CM9.0000000000001687
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.B 1207: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Ua VI Zs.425: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Sequencing the Complete Genome of COVID-19 Virus from Clinical Samples Using the Sanger Method.

Lu, Roujian / Niu, Peihua / Zhao, Li / Wang, Huijuan / Wang, Wenling / Tan, Wenjie

China CDC weekly

2021 Volume 2, Issue 25, Page(s) 447–452

Language	English
Publishing date	2021-07-28
Publishing country	China
Document type	Journal Article
ISSN	2096-7071
ISSN (online)	2096-7071
DOI	10.46234/ccdcw2020.088
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Development of two multiplex real-time PCR assays for simultaneous detection and differentiation of monkeypox virus IIa, IIb, and I clades and the B.1 lineage.

Huo, Shuting / Chen, Yuda / Lu, Roujian / Zhang, Zhongxian / Zhang, Gaoqian / Zhao, Li / Deng, Yao / Wu, Changcheng / Tan, Wenjie

Biosafety and health

2022

Abstract: An ongoing multicountry outbreak of monkeypox was reported in May 2022 with several deaths, affecting 107 countries of all six World Health Organization (WHO) regions. The WHO has declared the current monkeypox outbreak a Public Health Emergency of ... ...

Abstract	An ongoing multicountry outbreak of monkeypox was reported in May 2022 with several deaths, affecting 107 countries of all six World Health Organization (WHO) regions. The WHO has declared the current monkeypox outbreak a Public Health Emergency of International Concern. It is, thus, necessary to rapidly and accurately detect and distinguish different monkeypox virus (MPXV) clades. We designed primers and probes based on the alignment of 138 complete genomes of poxviruses. In Panel 1, we mixed one pair of primers and three probes to detect and differentiate the MPXV Western Africa (IIa, IIb clade) and Congo Basin (I clade) and other orthopoxviruses. In Panel 2, we mixed one pair of primers and two probes to detect the 2022 MPXV (B.1 lineage and its descendant lineages). In addition, we tested the specificity and sensitivity of the assay using real-time PCR. In Panel 1, the assay reproducibly identified various concentrations of two plasmids of the monkeypox virus, whereas other orthopoxviruses did not cross-react. In Panel 2, the probe annealed well to MPXV B.1 and showed the expected linearity. These two multiple real-time assays are inclusive and highly specific for identifying different clades of MPXV.
Language	English
Publishing date	2022-11-04
Publishing country	Netherlands
Document type	Journal Article
ISSN	2590-0536
ISSN (online)	2590-0536
DOI	10.1016/j.bsheal.2022.10.005
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Detection of SARS-CoV-2 in Different Types of Clinical Specimens.

Wang, Wenling / Xu, Yanli / Gao, Ruqin / Lu, Roujian / Han, Kai / Wu, Guizhen / Tan, Wenjie

JAMA

2020 Volume 323, Issue 18, Page(s) 1843–1844

MeSH term(s)	Adolescent ; Adult ; Aged ; Betacoronavirus/isolation & purification ; Bronchoalveolar Lavage Fluid/virology ; Bronchoscopy/statistics & numerical data ; COVID-19 ; Child ; Child, Preschool ; China ; Coronavirus Infections/blood ; Coronavirus Infections/urine ; Coronavirus Infections/virology ; Feces/virology ; Female ; Gene Dosage ; Genes, Viral ; Humans ; Male ; Middle Aged ; Nose/virology ; Open Reading Frames ; Pandemics ; Pharynx/virology ; Pneumonia, Viral/blood ; Pneumonia, Viral/urine ; Pneumonia, Viral/virology ; RNA, Viral/isolation & purification ; Real-Time Polymerase Chain Reaction ; SARS-CoV-2 ; Sputum/virology ; Viral Load ; Young Adult
Chemical Substances	RNA, Viral
Keywords	covid19
Language	English
Publishing date	2020-01-27
Publishing country	United States
Document type	Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
ZDB-ID	2958-0
ISSN	1538-3598 ; 0254-9077 ; 0002-9955 ; 0098-7484
ISSN (online)	1538-3598
ISSN	0254-9077 ; 0002-9955 ; 0098-7484
DOI	10.1001/jama.2020.3786
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ua VI Zs.88: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Detecting SARS-CoV-2 BA.2, BA.4, and BA.5 Variants Utilizing a Robust RT-RPA-CRISPR/Cas12a-Based Method - China, 2023.

Luo, Meihui / Pan, Yang / He, Yaqing / A, Ruhan / Wu, Changcheng / Huang, Baoying / Lu, Roujian / Zhao, Li / Peng, Bo / Ye, Fei / Wang, Huijuan / Chen, Yuda / Li, Zhen / Zhang, Daitao / Wang, Wenling / Tan, Wenjie

China CDC weekly

2023 Volume 5, Issue 26, Page(s) 584–591

Abstract: Introduction: Since 2019, numerous variants of concern for severe acute respiratory syndrome virus 2 (SARS-CoV-2) have emerged, leading to significant outbreaks. The development of novel, highly accurate, and rapid detection techniques for these new ... ...

Abstract	Introduction: Since 2019, numerous variants of concern for severe acute respiratory syndrome virus 2 (SARS-CoV-2) have emerged, leading to significant outbreaks. The development of novel, highly accurate, and rapid detection techniques for these new SARS-CoV-2 variants remains a primary focus in the ongoing efforts to control and prevent the coronavirus disease 2019 (COVID-19) pandemic. Methods: Reverse transcription-recombinase polymerase amplification combined with the clustered regularly interspaced short palindromic repeats-associated protein 12a (CRISPR/Cas12a) system was used to validate the detection of the Omicron BA.2, BA.4, and BA.5 variants of SARS-CoV-2. Results: Our results demonstrate that the CRISPR/Cas12a assay is capable of effectively detecting the SARS-CoV-2 BA.2, BA.4, and BA.5 variants with a limit of detection of 10, 1, and 10 copies/μL, respectively. Importantly, our assay successfully differentiated the three SARS-CoV-2 Omicron strains from one another. Additionally, we evaluated 46 SARS-CoV-2 positive clinical samples consisting of BA.2 ( Discussion: This research presents a swift and reliable CRISPR-based method that may be employed to track the emergence of novel SARS-CoV-2 variants.
Language	English
Publishing date	2023-06-29
Publishing country	China
Document type	Journal Article
ISSN	2096-7071
ISSN (online)	2096-7071
DOI	10.46234/ccdcw2023.113
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Characterization of whole genomes from recently emerging Mpox cases in several regions of China, 2023.

Wu, Changcheng / Cui, Lunbiao / Pan, Yang / Lv, Ziquan / Yao, Mingxiao / Wang, Wenling / Ye, Fei / Huo, Weibang / Zhao, Li / Huang, Baoying / Zhu, Fengcai / Lu, Roujian / Deng, Yao / Wang, Quanyi / Tan, Wenjie

Science China. Life sciences

2023

Language	English
Publishing date	2023-11-24
Publishing country	China
Document type	Letter
ZDB-ID	2546732-3
ISSN	1869-1889 ; 1674-7305
ISSN (online)	1869-1889
ISSN	1674-7305
DOI	10.1007/s11427-023-2485-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Discovery and identification of a novel canine coronavirus causing a diarrhea outbreak in Vulpes.

Liu, Yuting / Deng, Yao / Niu, Sheng / Zhu, Na / Song, Jingdong / Zhang, Xiaoshuang / Su, Weiting / Nie, Wenhui / Lu, Roujian / Irwin, David M / Gao, George Fu / Wang, Wenling / Wang, Qihui / Tan, Wenjie / Zhang, Shuyi

Science bulletin

2023 Volume 68, Issue 21, Page(s) 2598–2606

Abstract: Cross-species transmission of viruses from wildlife animal reservoirs, such as bats, poses a threat to human and domestic animal health. Previous studies have shown that domestic animals have important roles as intermediate hosts, enabling the ... ...

Abstract	Cross-species transmission of viruses from wildlife animal reservoirs, such as bats, poses a threat to human and domestic animal health. Previous studies have shown that domestic animals have important roles as intermediate hosts, enabling the transmission of genetically diverse coronaviruses from natural hosts to humans. Here, we report the identification and characterization of a novel canine coronavirus (VuCCoV), which caused an epidemic of acute diarrhea in Vulpes (foxes) in Shenyang, China. The epidemic started on November 8, 2019, and caused more than 39,600 deaths by January 1, 2022. Full-length viral genomic sequences were obtained from 15 foxes with diarrhea at the early stage of this outbreak. The VuCCoV genome shared more than 90% nucleotide identity with canine coronavirus (CCoV) for three of the four structural genes, with the S gene showing a larger amount of divergence. In addition, 67% (10/15) of the VuCCoV genomes contained an open reading frame (ORF3) gene, which was previously only detected in CCoV-I genomes. Notably, VuCCoV had only two to three amino acid differences at the partial RNA-dependent RNA polymerase (RdRp) level to bat CoV, suggesting a close genetic relationship. Therefore, these novel VuCCoV genomes represent a previously unsampled lineage of CCoVs. We also show that the VuCCoV spike protein binds to canine and fox aminopeptidase N (APN), which may allow this protein to serve as an entry receptor. In addition, cell lines were identified that are sensitive to VuCCoV using a pseudovirus system. These data highlight the importance of identifying the diversity and distribution of coronaviruses in domestic animals, which could mitigate future outbreaks that could threaten livestock, public health, and economic growth.
MeSH term(s)	Animals ; Dogs ; Humans ; Foxes ; Coronavirus, Canine/genetics ; Animals, Wild ; SARS-CoV-2/genetics ; Animals, Domestic ; Disease Outbreaks/veterinary ; Diarrhea/epidemiology
Language	English
Publishing date	2023-09-12
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2816140-3
ISSN	2095-9281 ; 2095-9273
ISSN (online)	2095-9281
ISSN	2095-9273
DOI	10.1016/j.scib.2023.09.011
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Epidemiology of Viruses Causing Pediatric Community Acquired Pneumonia in Shanghai During 2010-2020: What Happened Before and After the COVID-19 Outbreak?

Li, Fei / Zhang, Yuhan / Shi, Peng / Cao, Linfeng / Su, Liyun / Zhang, Yulan / Peng, Ke / Lu, Roujian / Tan, Wenjie / Shen, Jun

Infectious diseases and therapy

2021 Volume 11, Issue 1, Page(s) 165–174

Abstract: Introduction: Since the global outbreak of COVID-19, there has been a significant reduction in pediatric outpatient and emergency visits for infectious diseases. The purpose of this study was to analyze the changes in respiratory viruses in children ... ...

Abstract	Introduction: Since the global outbreak of COVID-19, there has been a significant reduction in pediatric outpatient and emergency visits for infectious diseases. The purpose of this study was to analyze the changes in respiratory viruses in children with community-acquired pneumonia (CAP) in Shanghai in the past 10 years, especially in the first year after COVID-19. Methods: We conducted a retrospective, observational study; the results for eight common respiratory viruses (respiratory syncytial virus (RSV), influenza virus A and B, parainfluenza virus 1-3 (PIV), adenovirus (ADV) and human metapneumovirus) tested by direct fluorescent antibody assays in hospitalized CAP cases in Children's Hospital of Fudan University during 2010-2020 were analyzed. Results: Of the 5544 hospitalized CAP patients included in this study, 20.2% (1125/5544) were positive for the eight respiratory viruses. The top three pathogens were RSV, PIV3 and ADV, detected from 9.8% (543/5544), 5.3% (294/5544) and 2.0% (111/5544) of the samples, respectively. RSV had the highest positive rates among children < 2 years old. In 2020, the detection rate of all viruses showed a sharp decline from February to August compared with the previous 9 years. When the Shanghai community reopened in August 2020, the detection rate of eight viruses rebounded significantly in September. Conclusions: These eight respiratory viruses, especially RSV and PIV, were important pathogens of CAP in Shanghai children in the past 10 years. The COVID-19 pandemic had a significant impact on the detection rates for eight respiratory viruses in children with CAP in Shanghai.
Language	English
Publishing date	2021-10-25
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2701611-0
ISSN	2193-6382 ; 2193-8229
ISSN (online)	2193-6382
ISSN	2193-8229
DOI	10.1007/s40121-021-00548-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Scutellaria baicalensis

Liu, Hongbo / Ye, Fei / Sun, Qi / Liang, Hao / Li, Chunmei / Li, Siyang / Lu, Roujian / Huang, Baoying / Tan, Wenjie / Lai, Luhua

Journal of enzyme inhibition and medicinal chemistry

2021 Volume 36, Issue 1, Page(s) 497–503

Abstract: COVID-19 has become a global pandemic and there is an urgent call for developing drugs against the virus (SARS-CoV-2). The 3C-like protease ( ... ...

Abstract	COVID-19 has become a global pandemic and there is an urgent call for developing drugs against the virus (SARS-CoV-2). The 3C-like protease (3CL
MeSH term(s)	Animals ; Antiviral Agents/pharmacology ; COVID-19/drug therapy ; COVID-19/enzymology ; COVID-19/virology ; Chlorocebus aethiops ; Coronavirus 3C Proteases/antagonists & inhibitors ; Drug Discovery ; Enzyme Inhibitors/pharmacology ; Flavanones/pharmacology ; Humans ; In Vitro Techniques ; Models, Molecular ; Plant Extracts/pharmacology ; Protease Inhibitors/pharmacology ; SARS-CoV-2/drug effects ; SARS-CoV-2/enzymology ; Scutellaria baicalensis ; Vero Cells ; Virus Replication/drug effects
Chemical Substances	Antiviral Agents ; Enzyme Inhibitors ; Flavanones ; Plant Extracts ; Protease Inhibitors ; Scutellaria baicalensis extract ; baicalein (49QAH60606) ; 3C-like proteinase, SARS-CoV-2 (EC 3.4.22.-) ; Coronavirus 3C Proteases (EC 3.4.22.28)
Language	English
Publishing date	2021-02-27
Publishing country	England
Document type	Journal Article
ZDB-ID	2082578-X
ISSN	1475-6374 ; 1475-6366
ISSN (online)	1475-6374
ISSN	1475-6366
DOI	10.1080/14756366.2021.1873977
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3004: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito