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Article ; Online: Fe-codoped carbon dots serving as a peroxidase mimic to generate in situ hydrogen peroxide for the visual detection of glucose.

Xie, Sijia / Zeng, Yating / Li, Jinfu / Lu, Xuemei / Xiong, Hai

2024

Abstract: Nanozyme technology has gained significant regard and been successfully implemented in various applications including chemical sensing, bio-medicine, and environmental monitoring. Fe-CDs were synthesized and characterized well in this study. As compared ... ...

Abstract	Nanozyme technology has gained significant regard and been successfully implemented in various applications including chemical sensing, bio-medicine, and environmental monitoring. Fe-CDs were synthesized and characterized well in this study. As compared to HRP (3.7 mM), the Fe-CDs exhibited a higher affinity towards H
Language	English
Publishing date	2024-02-16
Publishing country	Germany
Document type	Journal Article
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-024-05196-x
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Article ; Online: Adaptive Evolution of the Spike Protein in Coronaviruses.

Tang, Xiaolu / Qian, Zhaohui / Lu, Xuemei / Lu, Jian

Molecular biology and evolution

2023 Volume 40, Issue 4

Abstract: Coronaviruses are single-stranded, positive-sense RNA viruses that can infect many mammal and avian species. The Spike (S) protein of coronaviruses binds to a receptor on the host cell surface to promote viral entry. The interactions between the S ... ...

Abstract	Coronaviruses are single-stranded, positive-sense RNA viruses that can infect many mammal and avian species. The Spike (S) protein of coronaviruses binds to a receptor on the host cell surface to promote viral entry. The interactions between the S proteins of coronaviruses and receptors of host cells are extraordinarily complex, with coronaviruses from different genera being able to recognize the same receptor and coronaviruses from the same genus able to bind distinct receptors. As the coronavirus disease 2019 pandemic has developed, many changes in the S protein have been under positive selection by altering the receptor-binding affinity, reducing antibody neutralization activities, or affecting T-cell responses. It is intriguing to determine whether the selection pressure on the S gene differs between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other coronaviruses due to the host shift from nonhuman animals to humans. Here, we show that the S gene, particularly the S1 region, has experienced positive selection in both SARS-CoV-2 and other coronaviruses. Although the S1 N-terminal domain exhibits signals of positive selection in the pairwise comparisons in all four coronavirus genera, positive selection is primarily detected in the S1 C-terminal domain (the receptor-binding domain) in the ongoing evolution of SARS-CoV-2, possibly owing to the change in host settings and the widespread natural infection and SARS-CoV-2 vaccination in humans.
MeSH term(s)	Animals ; Humans ; COVID-19 ; SARS-CoV-2/genetics ; SARS-CoV-2/metabolism ; Spike Glycoprotein, Coronavirus/chemistry ; COVID-19 Vaccines ; Mammals/metabolism
Chemical Substances	spike protein, SARS-CoV-2 ; Spike Glycoprotein, Coronavirus ; COVID-19 Vaccines
Language	English
Publishing date	2023-05-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	998579-7
ISSN	1537-1719 ; 0737-4038
ISSN (online)	1537-1719
ISSN	0737-4038
DOI	10.1093/molbev/msad089
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Article ; Online: Bacterial genome reduction for optimal chassis of synthetic biology: a review.

Ma, Shuai / Su, Tianyuan / Lu, Xuemei / Qi, Qingsheng

Critical reviews in biotechnology

2023 Volume 44, Issue 4, Page(s) 660–673

Abstract: Bacteria with streamlined genomes, that harbor full functional genes for essential metabolic networks, are able to synthesize the desired products more effectively and thus have advantages as production platforms in industrial applications. To obtain ... ...

Abstract	Bacteria with streamlined genomes, that harbor full functional genes for essential metabolic networks, are able to synthesize the desired products more effectively and thus have advantages as production platforms in industrial applications. To obtain streamlined chassis genomes, a large amount of effort has been made to reduce existing bacterial genomes. This work falls into two categories: rational and random reduction. The identification of essential gene sets and the emergence of various genome-deletion techniques have greatly promoted genome reduction in many bacteria over the past few decades. Some of the constructed genomes possessed desirable properties for industrial applications, such as: increased genome stability, transformation capacity, cell growth, and biomaterial productivity. The decreased growth and perturbations in physiological phenotype of some genome-reduced strains may limit their applications as optimized cell factories. This review presents an assessment of the advancements made to date in bacterial genome reduction to construct optimal chassis for synthetic biology, including: the identification of essential gene sets, the genome-deletion techniques, the properties and industrial applications of artificially streamlined genomes, the obstacles encountered in constructing reduced genomes, and the future perspectives.
MeSH term(s)	Synthetic Biology/methods ; Genome, Bacterial ; Bacteria/genetics ; Bacteria/metabolism
Language	English
Publishing date	2023-06-28
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1042364-3
ISSN	1549-7801 ; 0738-8551
ISSN (online)	1549-7801
ISSN	0738-8551
DOI	10.1080/07388551.2023.2208285
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Article ; Online: Effect of safety and security equipment on patient and visitor violence towards nurses in multiple public hospitals of China during the COVID-19 pandemic: a retrospective, difference-in-difference analysis.

Hu, Yanzhen / Huang, Ju / Zhao, Dan / Zhang, Cheng / Xia, Jinghua / Lu, Xue-Mei

BMJ open

2024 Volume 14, Issue 1, Page(s) e078598

Abstract: Objectives: This study aimed to analyse whether safety and security equipment decreased patient and visitor violence (PVV) towards nurses in the COVID-19 period and quantify to what extent safety and security equipment affects PVV.: Design: ... ...

Abstract	Objectives: This study aimed to analyse whether safety and security equipment decreased patient and visitor violence (PVV) towards nurses in the COVID-19 period and quantify to what extent safety and security equipment affects PVV. Design: Controlled before and after study and difference-in-difference (DID) analysis. Setting: A large hospital medical group, consisting of three public tertiary teaching hospitals, namely, Xinjiekou Branch, Huilongguan Branch and Xinlongze Branch of Beijing Jishuitan Hospital, located in the west and north parts of Beijing, China. Participants: A panel of nine departments recruited using two-step sampling method, administered online surveys in 2021 and 2022. A total of 632 eligible nurses participated in the survey in 2021 and 725 eligible nurses in 2022. Measures: We assessed impacts of the safety and security equipment on the PVV. The policy had been enacted in June 2020, and the corresponding measures were established after mid-December 2020, and therefore, we use a DID design to evaluate changes in nurses' PVV incidence. Departments are classified as either department installed or non installed, and nurses are classified based on their department. Results: Within the treatment group, the incidence of physical PVV significantly decreased from 13.8% in 2020 to 2.0% in 2021. In the control group, the incidence of physical PVV increased from 0.6% in 2020 to 2.7% in 2021. The application of the safety and security equipment decreased the incidence of physical PVV by 13.93% (95% CI: -23.52% to -4.34%). In contrast, no difference was observed between the treatment and control groups for the incidence of psychological PVV (6.23%, 95% CI: -11.56% to 24.02%) and overall PVV (0.88, 95% CI: -20.90% to 22.66%). Conclusion: The safety and security equipment reduced the incidence of physical PVV towards nurses. For hospital managers in public hospitals, longer-term strategies roadmap for PVV prevention measures are needed to create a more supportive work environment in employees.
MeSH term(s)	Humans ; COVID-19/epidemiology ; COVID-19/prevention & control ; Retrospective Studies ; Pandemics/prevention & control ; Violence/prevention & control ; Hospitals, Public ; China/epidemiology ; Surveys and Questionnaires
Language	English
Publishing date	2024-01-31
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2599832-8
ISSN	2044-6055 ; 2044-6055
ISSN (online)	2044-6055
ISSN	2044-6055
DOI	10.1136/bmjopen-2023-078598
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Article ; Online: Prevalence of frailty among elderly patients with hip fracture in China: protocol for a systematic review and meta-analysis.

Xia, Jinghua / Li, Zhenyu / Zhao, Dan / Hu, Yanzhen / Lu, Xuemei

BMJ open

2023 Volume 13, Issue 10, Page(s) e072623

Abstract: Introduction: Frailty is a clinical syndrome characterised by a reduced ability to adapt to external stressors owing to a reduced physiological reserve, which is caused by degeneration of multiple organ systems. Frailty is particularly prevalent among ... ...

Abstract	Introduction: Frailty is a clinical syndrome characterised by a reduced ability to adapt to external stressors owing to a reduced physiological reserve, which is caused by degeneration of multiple organ systems. Frailty is particularly prevalent among patients with hip fractures. Research on frailty in China started late; thus, evidence regarding the prevalence of frailty among older patients with hip fracture in China is scarce. The aim of this systematic review and meta-analysis is to systematically search for available data on the prevalence of frailty among older patients with hip fracture in China, assess the pooled prevalence of frailty and describe the association between frailty and clinical outcomes. Method and analysis: We will systematically search electronic databases, including Web of Science, Embase, PubMed, the Cochrane Library, Chinese National Knowledge Infrastructure and Wanfang data Database, to identify studies on the prevalence of frailty in older patients with hip fracture. Two reviewers will independently identify eligible studies according to defined inclusion criteria and critically appraise them using the Joanna Briggs Institute's standardised critical appraisal tool. Data will be analysed using Stata V.12.0. Ethics and dissemination: Ethics approval is not required as this review will only include data from published sources. The results will be published in a peer-reviewed journal. Prospero registration number: CRD42022265321.
MeSH term(s)	Humans ; Aged ; Frailty/epidemiology ; Prevalence ; Systematic Reviews as Topic ; Meta-Analysis as Topic ; Hip Fractures/epidemiology ; China/epidemiology ; Review Literature as Topic
Language	English
Publishing date	2023-10-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2599832-8
ISSN	2044-6055 ; 2044-6055
ISSN (online)	2044-6055
ISSN	2044-6055
DOI	10.1136/bmjopen-2023-072623
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Article: The Nrf2 in Obesity: A Friend or Foe?

Xia, Yudong / Zhai, Xiaoying / Qiu, Yanning / Lu, Xuemei / Jiao, Yi

Antioxidants (Basel, Switzerland)

2022 Volume 11, Issue 10

Abstract: Obesity and its complications have become serious global health concerns recently and increasing work has been carried out to explicate the underlying mechanism of the disease development. The recognized correlations suggest oxidative stress and ... ...

Abstract	Obesity and its complications have become serious global health concerns recently and increasing work has been carried out to explicate the underlying mechanism of the disease development. The recognized correlations suggest oxidative stress and inflammation in expanding adipose tissue with excessive fat accumulation play important roles in the pathogenesis of obesity, as well as its associated metabolic syndromes. In adipose tissue, obesity-mediated insulin resistance strongly correlates with increased oxidative stress and inflammation. Nuclear factor erythroid 2-related factor 2 (Nrf2) has been described as a key modulator of antioxidant signaling, which regulates the transcription of various genes coding antioxidant enzymes and cytoprotective proteins. Furthermore, an increasing number of studies have demonstrated that Nrf2 is a pivotal target of obesity and its related metabolic disorders. However, its effects are controversial and even contradictory. This review aims to clarify the complicated interplay among Nrf2, oxidative stress, lipid metabolism, insulin signaling and chronic inflammation in obesity. Elucidating the implications of Nrf2 modulation on obesity would provide novel insights for potential therapeutic approaches in obesity and its comorbidities.
Language	English
Publishing date	2022-10-20
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox11102067
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Article ; Online: AcMYB10

Yu, Min / Xiong, Jinyu / Dong, Kun / Quan, Xin / Guo, Hao / Huo, Junwei / Qin, Dong / Wang, Yanchang / Lu, Xuemei / Zhu, Chenqiao

Genes

2024 Volume 15, Issue 1

Abstract: Light and temperature are key factors influencing the accumulation of anthocyanin in fruit crops. To assess the effects of fruit bagging during development and high post-ripening temperature on 'Hongyang' kiwifruit, we compared the pigmentation ... ...

Abstract	Light and temperature are key factors influencing the accumulation of anthocyanin in fruit crops. To assess the effects of fruit bagging during development and high post-ripening temperature on 'Hongyang' kiwifruit, we compared the pigmentation phenotypes and expression levels of anthocyanin-related genes between bagged and unbagged treatments, and between 25 °C and 37 °C postharvest storage temperatures. Both the bagging and 25 °C treatments showed better pigmentation phenotypes with higher anthocyanin concentrations. The results of the qRT-PCR analysis revealed that the gene expression levels of
MeSH term(s)	Anthocyanins ; Fruit/genetics ; Temperature ; Flavonoids ; Actinidia/genetics ; Saccharomyces cerevisiae
Chemical Substances	Anthocyanins ; Flavonoids
Language	English
Publishing date	2024-01-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527218-4
ISSN	2073-4425 ; 2073-4425
ISSN (online)	2073-4425
ISSN	2073-4425
DOI	10.3390/genes15010097
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Article ; Online: Some novel features of strong promoters discovered in Cytophaga hutchinsonii.

Fan, Guoqing / Song, Wenxia / Guan, Zhiwei / Zhang, Weican / Lu, Xuemei

Applied microbiology and biotechnology

2022 Volume 106, Issue 7, Page(s) 2529–2540

Abstract: Cytophaga hutchinsonii is an important Gram-negative bacterium belonging to the Bacteroides phylum that can efficiently degrade cellulose. But the promoter that mediates the initiation of gene transcription has been unknown for a long time. In this study, ...

Abstract	Cytophaga hutchinsonii is an important Gram-negative bacterium belonging to the Bacteroides phylum that can efficiently degrade cellulose. But the promoter that mediates the initiation of gene transcription has been unknown for a long time. In this study, we determined the transcription start site (TSS) of C. hutchinsonii by 5' rapid amplification of cDNA ends (5'RACE). The promoter structure was first identified as TAAT and TATTG which are located -5 and -31 bp upstream of TSS, respectively. The function of -5 and -31 regions and the spacer length of the promoter P
MeSH term(s)	Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cellulase/metabolism ; Cellulose/metabolism ; Cytophaga/genetics ; Cytophaga/metabolism
Chemical Substances	Bacterial Proteins ; Cellulose (9004-34-6) ; Cellulase (EC 3.2.1.4)
Language	English
Publishing date	2022-03-23
Publishing country	Germany
Document type	Journal Article
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-022-11869-3
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Zs.A 2229: Show issues

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Article ; Online: Cytophaga hutchinsonii chu_2177, encoding the O-antigen ligase, is essential for cellulose degradation.

Tan, Yahong / Song, Wenxia / Gao, Lijuan / Zhang, Weican / Lu, Xuemei

Journal of microbiology (Seoul, Korea)

2022 Volume 60, Issue 4, Page(s) 364–374

Abstract: Cytophaga hutchinsonii can efficiently degrade crystalline cellulose, in which the cell surface cellulases secreted by the type IX secretion system (T9SS) play important roles, but the degradation mechanism remains unclear, and the anchor mechanism of ... ...

Abstract	Cytophaga hutchinsonii can efficiently degrade crystalline cellulose, in which the cell surface cellulases secreted by the type IX secretion system (T9SS) play important roles, but the degradation mechanism remains unclear, and the anchor mechanism of cellulases on the outer membrane in C. hutchinsonii has not been studied. Here, chu_2177 was identified by transposon mutagenesis and was proved to be indispensable for cellulose utilization in C. hutchinsonii. Disruption of chu_2177 resulted in O-antigen deficiency and chu_177 could confer O-antigen ligase activity upon an Escherichia coli waal mutant, indicating that chu_2177 encoded the O-ntigen ligase. Moreover, deletion of chu_2177 caused defects in cellulose utilization, cell motility, biofilm formation, and stress resistance. Further study showed that the endoglucanase activity was markedly decreased in the outer membrane but was increased in the culture fluid without chu_2177. Western blot proved that endoglucanase CHU_1336 was not located on the outer membrane but was released in the culture fluid of the Δ2177 mutant. Further proteomics analysis showed that many cargo proteins of T9SS were missing in the outer membrane of the Δ2177 mutant. Our study revealed that the deletion of chu_2177 affected the localization of many T9SS cargo proteins including cellulases on the outer membrane of C. hutchinsonii.
MeSH term(s)	Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cellulose/metabolism ; Cytophaga/genetics ; Cytophaga/metabolism ; Ligases/metabolism ; O Antigens/metabolism
Chemical Substances	Bacterial Proteins ; O Antigens ; Cellulose (9004-34-6) ; Ligases (EC 6.-)
Language	English
Publishing date	2022-01-07
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	2012399-1
ISSN	1976-3794 ; 1225-8873
ISSN (online)	1976-3794
ISSN	1225-8873
DOI	10.1007/s12275-022-1531-3
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Zs.A 5211: Show issues

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Article ; Online: Reduction of the Bacterial Genome by Transposon-Mediated Random Deletion.

Ma, Shuai / Su, Tianyuan / Liu, Jinming / Lu, Xuemei / Qi, Qingsheng

ACS synthetic biology

2022 Volume 11, Issue 2, Page(s) 668–677

Abstract: Genome reduction is an important strategy in synthetic biology for constructing functional chassis cells or minimal genomes. However, the limited knowledge of complex gene functions and interactions makes genome reduction by rational design encounter a ... ...

Abstract	Genome reduction is an important strategy in synthetic biology for constructing functional chassis cells or minimal genomes. However, the limited knowledge of complex gene functions and interactions makes genome reduction by rational design encounter a bottleneck. Here, we present an iterative and random genome reduction method for
MeSH term(s)	CRISPR-Cas Systems/genetics ; Escherichia coli/genetics ; Gene Library ; Genome, Bacterial/genetics ; Genomics ; Synthetic Biology
Language	English
Publishing date	2022-02-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2161-5063
ISSN (online)	2161-5063
DOI	10.1021/acssynbio.1c00353
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