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  1. Article: Fusarium oxysporum f.sp. radicis-lycopersici induces distinct transcriptome reprogramming in resistant and susceptible isogenic tomato lines

    Manzo, Daniele / Alberto Ferrarini / Astolfo Zoina / Daniela D’Esposito / Francesca Ferriello / Gerardo Puopolo / Luca Tardella / Maria Raffaella Ercolano

    BMC plant biology. 2016 Dec., v. 16, no. 1

    2016  

    Abstract: BACKGROUND: Fusarium oxysporum f.sp. radicis-lycopersici (FORL) is one of the most destructive necrotrophic pathogens affecting tomato crops, causing considerable field and greenhouse yield losses. Despite such major economic impact, little is known ... ...

    Abstract BACKGROUND: Fusarium oxysporum f.sp. radicis-lycopersici (FORL) is one of the most destructive necrotrophic pathogens affecting tomato crops, causing considerable field and greenhouse yield losses. Despite such major economic impact, little is known about the molecular mechanisms regulating Fusarium oxysporum f.sp. radicis-lycopersici resistance in tomato. RESULTS: A transcriptomic experiment was carried out in order to investigate the main mechanisms of FORL response in resistant and susceptible isogenic tomato lines. Microarray analysis at 15 DPI (days post inoculum) revealed a distinct gene expression pattern between the two genotypes in the inoculated vs non-inoculated conditions. A model of plant response both for compatible and incompatible reactions was proposed. In particular, in the incompatible interaction an activation of defense genes related to secondary metabolite production and tryptophan metabolism was observed. Moreover, maintenance of the cell osmotic potential after the FORL challenging was mediated by a dehydration-induced protein. As for the compatible interaction, activation of an oxidative burst mediated by peroxidases and a cytochrome monooxygenase induced cell degeneration and necrosis. CONCLUSIONS: Our work allowed comprehensive understanding of the molecular basis of the tomato-FORL interaction. The result obtained emphasizes a different transcriptional reaction between the resistant and the susceptible genotype to the FORL challenge. Our findings could lead to the improvement in disease control strategies.
    Keywords crops ; disease control ; economic impact ; Fusarium oxysporum ; gene expression regulation ; genes ; genotype ; greenhouses ; inoculum ; metabolism ; microarray technology ; models ; necrosis ; osmotic pressure ; pathogens ; peroxidases ; plant response ; secondary metabolites ; tomatoes ; transcription (genetics) ; transcriptome ; transcriptomics ; tryptophan
    Language English
    Dates of publication 2016-12
    Size p. 53.
    Publishing place BioMed Central
    Document type Article
    ISSN 1471-2229
    DOI 10.1186/s12870-016-0740-5
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Tomato genome-wide transcriptional responses to Fusarium wilt and Tomato Mosaic Virus.

    Giuseppe Andolfo / Francesca Ferriello / Luca Tardella / Alberto Ferrarini / Loredana Sigillo / Luigi Frusciante / Maria Raffaella Ercolano

    PLoS ONE, Vol 9, Iss 5, p e

    2014  Volume 94963

    Abstract: Since gene expression approaches constitute a starting point for investigating plant-pathogen systems, we performed a transcriptional analysis to identify a set of genes of interest in tomato plants infected with F. oxysporum f. sp. lycopersici (Fol) and ...

    Abstract Since gene expression approaches constitute a starting point for investigating plant-pathogen systems, we performed a transcriptional analysis to identify a set of genes of interest in tomato plants infected with F. oxysporum f. sp. lycopersici (Fol) and Tomato Mosaic Virus (ToMV). Differentially expressed tomato genes upon inoculation with Fol and ToMV were identified at two days post-inoculation. A large overlap was found in differentially expressed genes throughout the two incompatible interactions. However, Gene Ontology enrichment analysis evidenced specific categories in both interactions. Response to ToMV seems more multifaceted, since more than 70 specific categories were enriched versus the 30 detected in Fol interaction. In particular, the virus stimulated the production of an invertase enzyme that is able to redirect the flux of carbohydrates, whereas Fol induced a homeostatic response to prevent the fungus from killing cells. Genomic mapping of transcripts suggested that specific genomic regions are involved in resistance response to pathogen. Coordinated machinery could play an important role in prompting the response, since 60% of pathogen receptor genes (NB-ARC-LRR, RLP, RLK) were differentially regulated during both interactions. Assessment of genomic gene expression patterns could help in building up models of mediated resistance responses.
    Keywords Medicine ; R ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2014-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: Unraveling the complexity of transcriptomic, metabolomic and quality environmental response of tomato fruit

    D’Esposito, Daniela / Adriana Sacco / Alessandra Dal Molin / Amalia Barone / Andrea Minio / Francesca Ferriello / Gianfranco Diretto / Giovanni Giuliano / Luca Tardella / Luigi Frusciante / Maria Raffaella Ercolano / Massimo Delledonne / Rossella Di Monaco / Silvana Cavella

    BMC plant biology. 2017 Dec., v. 17, no. 1

    2017  

    Abstract: BACKGROUND: The environment has a profound influence on the organoleptic quality of tomato (Solanum lycopersicum) fruit, the extent of which depends on a well-regulated and dynamic interplay among genes, metabolites and sensorial attributes. We used a ... ...

    Abstract BACKGROUND: The environment has a profound influence on the organoleptic quality of tomato (Solanum lycopersicum) fruit, the extent of which depends on a well-regulated and dynamic interplay among genes, metabolites and sensorial attributes. We used a systems biology approach to elucidate the complex interacting mechanisms regulating the plasticity of sensorial traits. To investigate environmentally challenged transcriptomic and metabolomic remodeling and evaluate the organoleptic consequences of such variations we grown three tomato varieties, Heinz 1706, whose genome was sequenced as reference and two “local” ones, San Marzano and Vesuviano in two different locations of Campania region (Italy). RESULTS: Responses to environment were more pronounced in the two “local” genotypes, rather than in the Heinz 1706. The overall genetic composition of each genotype, acting in trans, modulated the specific response to environment. Duplicated genes and transcription factors, establishing different number of network connections by gaining or losing links, play a dominant role in shaping organoleptic profile. The fundamental role of cell wall metabolism in tuning all the quality attributes, including the sensorial perception, was also highlighted. CONCLUSIONS: Although similar fruit-related quality processes are activated in the same environment, different tomato genotypes follow distinct transcriptomic, metabolomic and sensorial trajectories depending on their own genetic makeup.
    Keywords cell walls ; genes ; genotype ; metabolism ; metabolites ; metabolomics ; sensory properties ; Solanum lycopersicum ; tomatoes ; transcription (genetics) ; transcription factors ; transcriptomics ; Italy
    Language English
    Dates of publication 2017-12
    Size p. 66.
    Publishing place BioMed Central
    Document type Article
    ISSN 1471-2229
    DOI 10.1186/s12870-017-1008-4
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Identification of relevant conformational epitopes on the HER2 oncoprotein by using Large Fragment Phage Display (LFPD).

    Federico Gabrielli / Roberto Salvi / Chiara Garulli / Cristina Kalogris / Serena Arima / Luca Tardella / Paolo Monaci / Serenella M Pupa / Elda Tagliabue / Maura Montani / Elena Quaglino / Lorenzo Stramucci / Claudia Curcio / Cristina Marchini / Augusto Amici

    PLoS ONE, Vol 8, Iss 3, p e

    2013  Volume 58358

    Abstract: We developed a new phage-display based approach, the Large Fragment Phage Display (LFPD), that can be used for mapping conformational epitopes on target molecules of immunological interest. LFPD uses a simplified and more effective phage-display approach ...

    Abstract We developed a new phage-display based approach, the Large Fragment Phage Display (LFPD), that can be used for mapping conformational epitopes on target molecules of immunological interest. LFPD uses a simplified and more effective phage-display approach in which only a limited set of larger fragments (about 100 aa in length) are expressed on the phage surface. Using the human HER2 oncoprotein as a target, we identified novel B-cell conformational epitopes. The same homologous epitopes were also detected in rat HER2 and all corresponded to the epitopes predicted by computational analysis (PEPITO software), showing that LFPD gives reproducible and accurate results. Interestingly, these newly identified HER2 epitopes seem to be crucial for an effective immune response against HER2-overexpressing breast cancers and might help discriminating between metastatic breast cancer and early breast cancer patients. Overall, the results obtained in this study demonstrated the utility of LFPD and its potential application to the detection of conformational epitopes on many other molecules of interest, as well as, the development of new and potentially more effective B-cell conformational epitopes based vaccines.
    Keywords Medicine ; R ; Science ; Q
    Subject code 500
    Language English
    Publishing date 2013-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

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