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  1. Article ; Online: Challenges and opportunities for modeling aging and cancer.

    Anczuków, Olga / Airhart, Susie / Chuang, Jeffrey H / Coussens, Lisa M / Kuchel, George A / Korstanje, Ron / Li, Sheng / Lucido, Anna Lisa / McAllister, Sandra S / Politi, Katerina / Polyak, Kornelia / Ratliff, Timothy / Ren, Gary / Trowbridge, Jennifer J / Ucar, Duygu / Palucka, Karolina

    Cancer cell

    2023  Volume 41, Issue 4, Page(s) 641–645

    Abstract: Age is among the main risk factors for cancer, and any cancer study in adults is faced with an aging tissue and organism. Yet, pre-clinical studies are carried out using young mice and are not able to address the impact of aging and associated ... ...

    Abstract Age is among the main risk factors for cancer, and any cancer study in adults is faced with an aging tissue and organism. Yet, pre-clinical studies are carried out using young mice and are not able to address the impact of aging and associated comorbidities on disease biology and treatment outcomes. Here, we discuss the limitations of current mouse cancer models and suggest strategies for developing novel models to address these major gaps in knowledge and experimental approaches.
    MeSH term(s) Animals ; Mice ; Aging ; Neoplasms/genetics ; Disease Models, Animal ; Risk Factors
    Language English
    Publishing date 2023-03-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2078448-X
    ISSN 1878-3686 ; 1535-6108
    ISSN (online) 1878-3686
    ISSN 1535-6108
    DOI 10.1016/j.ccell.2023.03.006
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  2. Article ; Online: Mapping systemic lupus erythematosus heterogeneity at the single-cell level.

    Nehar-Belaid, Djamel / Hong, Seunghee / Marches, Radu / Chen, Guo / Bolisetty, Mohan / Baisch, Jeanine / Walters, Lynnette / Punaro, Marilynn / Rossi, Robert J / Chung, Cheng-Han / Huynh, Richie P / Singh, Prashant / Flynn, William F / Tabanor-Gayle, Joy-Ann / Kuchipudi, Navya / Mejias, Asuncion / Collet, Magalie A / Lucido, Anna Lisa / Palucka, Karolina /
    Robson, Paul / Lakshminarayanan, Santhanam / Ramilo, Octavio / Wright, Tracey / Pascual, Virginia / Banchereau, Jacques F

    Nature immunology

    2020  Volume 21, Issue 9, Page(s) 1094–1106

    Abstract: Patients with systemic lupus erythematosus (SLE) display a complex blood transcriptome whose cellular origin is poorly resolved. Using single-cell RNA sequencing, we profiled ~276,000 peripheral blood mononuclear cells from 33 children with SLE with ... ...

    Abstract Patients with systemic lupus erythematosus (SLE) display a complex blood transcriptome whose cellular origin is poorly resolved. Using single-cell RNA sequencing, we profiled ~276,000 peripheral blood mononuclear cells from 33 children with SLE with different degrees of disease activity and 11 matched controls. Increased expression of interferon-stimulated genes (ISGs) distinguished cells from children with SLE from healthy control cells. The high ISG expression signature (ISG
    MeSH term(s) Adolescent ; Adult ; Cells, Cultured ; Child ; Cohort Studies ; Disease Progression ; Female ; Gene Expression Profiling ; Humans ; Interferons/genetics ; Leukocytes, Mononuclear/physiology ; Lupus Erythematosus, Systemic/genetics ; Male ; Sequence Analysis, RNA ; Severity of Illness Index ; Single-Cell Analysis/methods ; Transcriptome
    Chemical Substances Interferons (9008-11-1)
    Language English
    Publishing date 2020-08-03
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2016987-5
    ISSN 1529-2916 ; 1529-2908
    ISSN (online) 1529-2916
    ISSN 1529-2908
    DOI 10.1038/s41590-020-0743-0
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  3. Article ; Online: Isolation of functional presynaptic complexes from CNS neurons: a cell-free preparation for the study of presynaptic compartments In vitro.

    Lucido, Anna Lisa / Gopalakrishnan, Gopakumar / Yam, Patricia T / Colman, David R / Lennox, R Bruce

    ACS chemical neuroscience

    2010  Volume 1, Issue 8, Page(s) 535–541

    Abstract: The difficulty in developing successful treatments to facilitate nerve regeneration has prompted a number of new in vitro experimental methods. We have recently shown that functional presynaptic boutons can be formed when neuronal cells are cocultured ... ...

    Abstract The difficulty in developing successful treatments to facilitate nerve regeneration has prompted a number of new in vitro experimental methods. We have recently shown that functional presynaptic boutons can be formed when neuronal cells are cocultured with surface-modified artificial substrates including poly(d-lysine)-coated beads and supported lipid bilayer-coated beads (Lucido(2009) J. Neurosci.29, 12449-12466; Gopalakrishnan(2010) ACS Chem. Neurosci.1, 86-94). We demonstrate here, using confocal microscopy combined with immunocytochemistry, that it is possible to isolate such in vitro presynaptic endings in an exclusive fashion onto glass substrates through a simple "sandwich/lift-off" technique (Perez(2006) Adv. Funct. Mater.16, 306-312). Isolated presynaptic complexes are capable of releasing and recycling neurotransmitter in response to an external chemical trigger. These bead-presynaptic complexes are facile to prepare and are readily dispersible in solution. They are thus compatible with many experimental methods whose focus is the study of the neuronal presynaptic compartment.
    MeSH term(s) Animals ; Cell Fractionation/instrumentation ; Cell Fractionation/methods ; Cell-Free System ; Cells, Cultured ; Central Nervous System/cytology ; Coated Materials, Biocompatible ; Exocytosis ; Fluorescent Dyes/analysis ; Hippocampus/cytology ; Hippocampus/embryology ; Microscopy, Confocal ; Microspheres ; Nerve Tissue Proteins/analysis ; Neurons/chemistry ; Neurons/metabolism ; Neurons/ultrastructure ; Polylysine ; Polystyrenes ; Presynaptic Terminals/chemistry ; Presynaptic Terminals/metabolism ; Presynaptic Terminals/ultrastructure ; Primary Cell Culture/methods ; Rats ; Rats, Sprague-Dawley
    Chemical Substances Coated Materials, Biocompatible ; Fluorescent Dyes ; Nerve Tissue Proteins ; Polystyrenes ; Polylysine (25104-18-1) ; polystyrene sulfonic acid (70KO0R01RY)
    Language English
    Publishing date 2010-06-24
    Publishing country United States
    Document type Letter
    ISSN 1948-7193
    ISSN (online) 1948-7193
    DOI 10.1021/cn100048z
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  4. Article: Lipid bilayer membrane-triggered presynaptic vesicle assembly.

    Gopalakrishnan, Gopakumar / Thostrup, Peter / Rouiller, Isabelle / Lucido, Anna Lisa / Belkaïd, Wiam / Colman, David R / Lennox, R Bruce

    ACS chemical neuroscience

    2009  Volume 1, Issue 2, Page(s) 86–94

    Abstract: The formation of functional synapses on artificial substrates is a very important step in the development of engineered in vitro neural networks. Spherical supported bilayer lipid membranes (SS-BLMs) are used here as a novel substrate to demonstrate ... ...

    Abstract The formation of functional synapses on artificial substrates is a very important step in the development of engineered in vitro neural networks. Spherical supported bilayer lipid membranes (SS-BLMs) are used here as a novel substrate to demonstrate presynaptic vesicle accumulation at an in vitro synaptic junction. Confocal fluorescence microscopy, cryo-transmission electron microscopy (cryo-TEM), and fluorescence recovery after photobleaching (FRAP) experiments have been used to characterize the SS-BLMs. Conventional immunocytochemistry combined with confocal fluorescence microscopy was used to observe the formation of presynaptic vesicles at the neuron-SS-BLM contacts. These results indicate that lipid phases may play a role in the observed phenomenon, in addition to the chemical and electrostatic interactions between the neurons and SS-BLMs. The biocompatibility of lipid bilayers along with their membrane tunability makes the suggested approach a useful "toolkit" for many neuroengineering applications including artificial synapse formation and synaptogenesis in vivo.
    MeSH term(s) Animals ; Coculture Techniques ; Cryoelectron Microscopy ; Hippocampus/cytology ; Immunohistochemistry ; Lipid Bilayers ; Lipids/chemistry ; Microscopy, Confocal ; Microscopy, Electron, Transmission ; Nanoparticles ; Rats ; Receptors, Presynaptic/biosynthesis ; Receptors, Presynaptic/drug effects ; Synaptic Vesicles/drug effects
    Chemical Substances Lipid Bilayers ; Lipids ; Receptors, Presynaptic
    Language English
    Publishing date 2009-10-15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1948-7193
    ISSN 1948-7193
    DOI 10.1021/cn900011n
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  5. Article: Prolonged morphine treatment selectively increases membrane recruitment of delta-opioid receptors in mouse basal ganglia.

    Lucido, Anna Lisa / Morinville, Anne / Gendron, Louis / Stroh, Thomas / Beaudet, Alain

    Journal of molecular neuroscience : MN

    2005  Volume 25, Issue 3, Page(s) 207–214

    Abstract: In recent years, we demonstrated that prolonged (48-h) treatment of rats or mice with selective m-opioid receptor ((mu)OR) agonists induced a translocation of delta-opioid receptors ((delta)ORs) from intracellular compartments to neuronal plasma ... ...

    Abstract In recent years, we demonstrated that prolonged (48-h) treatment of rats or mice with selective m-opioid receptor ((mu)OR) agonists induced a translocation of delta-opioid receptors ((delta)ORs) from intracellular compartments to neuronal plasma membranes in the dorsal horn of the spinal cord. It remained to be determined whether this phenomenon also occurred in the brain. To resolve this issue, we analyzed by immunogold histochemistry the subcellular distribution of (delta)ORs in the nucleus accumbens, dorsal neostriatum, and frontal cortex in mice treated or not with morphine (48 h). We observed that prolonged treatment with morphine induced a translocation of (delta)ORs from intracellular to subplasmalemmal and membrane compartments in dendrites from both the nucleus accumbens and the dorsal neostriatum but not from the frontal cortex. We propose that this (mu)OR-(delta)OR interaction might prolong and modulate the sensitivity of neurons to opiates in specific target regions.
    MeSH term(s) Analgesics, Opioid/pharmacology ; Animals ; Cell Membrane/metabolism ; Cell Membrane/ultrastructure ; Female ; Frontal Lobe/cytology ; Frontal Lobe/drug effects ; Frontal Lobe/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Microscopy, Electron ; Morphine/pharmacology ; Neostriatum/cytology ; Neostriatum/drug effects ; Neostriatum/metabolism ; Neurons/drug effects ; Neurons/metabolism ; Neurons/ultrastructure ; Nucleus Accumbens/cytology ; Nucleus Accumbens/drug effects ; Nucleus Accumbens/metabolism ; Protein Transport/drug effects ; Receptors, Opioid, delta/metabolism
    Chemical Substances Analgesics, Opioid ; Receptors, Opioid, delta ; Morphine (76I7G6D29C)
    Language English
    Publishing date 2005
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1043392-2
    ISSN 0895-8696
    ISSN 0895-8696
    DOI 10.1385/JMN:25:3:207
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    Zs.A 3006: Show issues Location:
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  6. Article ; Online: Morphine and pain-related stimuli enhance cell surface availability of somatic delta-opioid receptors in rat dorsal root ganglia.

    Gendron, Louis / Lucido, Anna Lisa / Mennicken, Françoise / O'Donnell, Dajan / Vincent, Jean-Pierre / Stroh, Thomas / Beaudet, Alain

    The Journal of neuroscience : the official journal of the Society for Neuroscience

    2006  Volume 26, Issue 3, Page(s) 953–962

    Abstract: The present study demonstrates that perikaryaldelta-opioid receptors (deltaORs) in rat dorsal root ganglion (DRG) neurons bind and internalize opioid ligands circulating in the CSF. Using confocal and electron microscopy, we found that prolonged morphine ...

    Abstract The present study demonstrates that perikaryaldelta-opioid receptors (deltaORs) in rat dorsal root ganglion (DRG) neurons bind and internalize opioid ligands circulating in the CSF. Using confocal and electron microscopy, we found that prolonged morphine treatment increased the cell surface density of these perikaryal deltaORs and, by way of consequence, receptor-mediated internalization of the fluorescent deltorphin (DLT) analog omega-Bodipy 576/589 deltorphin-I 5-aminopentylamide (Fluo-DLT) in all three types of DRG neurons (small, medium, and large). In contrast, chronic inflammatory pain induced by the injection of complete Freund's adjuvant (CFA) into one hindpaw selectively increased Fluo-DLT internalization in small and medium-sized DRG neurons ipsilateral to the inflammation. Based on our previous studies in the spinal cord of mu-opioid receptor (muOR) knock-out mice, it may be assumed that the enhanced membrane recruitment of deltaORs observed after sustained morphine is attributable to stimulation of muORs. However, the selectivity of the effect induced by inflammatory pain suggests that it involves a different mechanism, namely a modality-specific and pain-related activation of C and Adelta fibers. Indeed, stimulation by capsaicin of transient receptor potential vanilloid 1 receptors, which are selectively expressed by small diameter (< 600 microm2) DRG neurons, increased Fluo-DLT internalization exclusively in this cell population. The present results, therefore, demonstrate that DRG neurons express perikaryal deltaORs accessible to CSF-circulating ligands and that the density and, hence, presumably also the responsiveness, of these receptors may be modulated by both pain-related stimuli and sustained exposure to muOR agonists.
    MeSH term(s) Animals ; Cell Membrane/drug effects ; Cell Membrane/metabolism ; Cells, Cultured ; Dose-Response Relationship, Drug ; Ganglia, Spinal/cytology ; Ganglia, Spinal/drug effects ; Ganglia, Spinal/metabolism ; Male ; Morphine/pharmacology ; Morphine/therapeutic use ; Pain/drug therapy ; Pain/metabolism ; Pain Measurement/drug effects ; Pain Measurement/methods ; Rats ; Rats, Sprague-Dawley ; Receptors, Opioid, delta/analysis ; Receptors, Opioid, delta/biosynthesis ; Receptors, Opioid, delta/physiology
    Chemical Substances Receptors, Opioid, delta ; Morphine (76I7G6D29C)
    Language English
    Publishing date 2006-01-12
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 604637-x
    ISSN 1529-2401 ; 0270-6474
    ISSN (online) 1529-2401
    ISSN 0270-6474
    DOI 10.1523/JNEUROSCI.3598-05.2006
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  7. Article ; Online: Rapid assembly of functional presynaptic boutons triggered by adhesive contacts.

    Lucido, Anna Lisa / Suarez Sanchez, Fernando / Thostrup, Peter / Kwiatkowski, Adam V / Leal-Ortiz, Sergio / Gopalakrishnan, Gopakumar / Liazoghli, Dalinda / Belkaid, Wiam / Lennox, R Bruce / Grutter, Peter / Garner, Craig C / Colman, David R

    The Journal of neuroscience : the official journal of the Society for Neuroscience

    2009  Volume 29, Issue 40, Page(s) 12449–12466

    Abstract: CNS synapse assembly typically follows after stable contacts between "appropriate" axonal and dendritic membranes are made. We show that presynaptic boutons selectively form de novo following neuronal fiber adhesion to beads coated with poly-d-lysine ( ... ...

    Abstract CNS synapse assembly typically follows after stable contacts between "appropriate" axonal and dendritic membranes are made. We show that presynaptic boutons selectively form de novo following neuronal fiber adhesion to beads coated with poly-d-lysine (PDL), an artificial cationic polypeptide. As demonstrated by atomic force and live confocal microscopy, functional presynaptic boutons self-assemble as rapidly as 1 h after bead contact, and are found to contain a variety of proteins characteristic of presynaptic endings. Interestingly, presynaptic compartment assembly does not depend on the presence of a biological postsynaptic membrane surface. Rather, heparan sulfate proteoglycans, including syndecan-2, as well as others possibly adsorbed onto the bead matrix or expressed on the axon surface, are required for assembly to proceed by a mechanism dependent on the dynamic reorganization of F-actin. Our results indicate that certain (but not all) nonspecific cationic molecules like PDL, with presumably electrostatically mediated adhesive properties, can effectively bypass cognate and natural postsynaptic ligands to trigger presynaptic assembly in the absence of specific target recognition. In contrast, we find that postsynaptic compartment assembly depends on the prior presence of a mature presynaptic ending.
    MeSH term(s) Animals ; Axons ; Cell Adhesion ; Cells, Cultured ; Hippocampus/cytology ; Hippocampus/embryology ; Hippocampus/metabolism ; Membrane Proteins/metabolism ; Microscopy, Atomic Force ; Microscopy, Confocal ; Presynaptic Terminals/metabolism ; Presynaptic Terminals/ultrastructure ; Rats ; Rats, Sprague-Dawley ; Synaptic Vesicles/metabolism ; Synaptic Vesicles/ultrastructure
    Chemical Substances Membrane Proteins
    Language English
    Publishing date 2009-10-07
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 604637-x
    ISSN 1529-2401 ; 0270-6474
    ISSN (online) 1529-2401
    ISSN 0270-6474
    DOI 10.1523/JNEUROSCI.1381-09.2009
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