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  1. Article ; Online: Antiviral mechanisms of sorafenib against foot-and-mouth disease virus via c-RAF and AKT/PI3K pathways.

    Theerawatanasirikul, Sirin / Lueangaramkul, Varanya / Semkum, Ploypailin / Lekcharoensuk, Porntippa

    Veterinary research communications

    2023  Volume 48, Issue 1, Page(s) 329–343

    Abstract: Foot-and-mouth disease virus (FMDV) is a highly contagious pathogen that poses a significant threat to the global livestock industry. However, specific antiviral treatments against FMDV are currently unavailable. This study aimed to evaluate the ... ...

    Abstract Foot-and-mouth disease virus (FMDV) is a highly contagious pathogen that poses a significant threat to the global livestock industry. However, specific antiviral treatments against FMDV are currently unavailable. This study aimed to evaluate the antiviral activity of anticancer drugs, including kinase and non-kinase inhibitors against FMDV replication in BHK-21 cells. Sorafenib, a multi-kinase inhibitor, demonstrated a significant dose-dependent reduction in FMDV replication. It exhibited a half maximal effective concentration (EC50) value of 2.46 µM at the pre-viral entry stage and 2.03 µM at the post-viral entry stage. Further intracellular assays revealed that sorafenib effectively decreased 3D
    MeSH term(s) Animals ; Foot-and-Mouth Disease Virus ; Sorafenib/pharmacology ; Sorafenib/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Phosphatidylinositol 3-Kinases/pharmacology ; Proto-Oncogene Proteins c-akt/metabolism ; Proto-Oncogene Proteins c-akt/pharmacology ; Foot-and-Mouth Disease ; Molecular Docking Simulation ; Cell Line ; Antiviral Agents/pharmacology ; Virus Replication
    Chemical Substances Sorafenib (9ZOQ3TZI87) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Antiviral Agents
    Language English
    Publishing date 2023-09-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 406735-6
    ISSN 1573-7446 ; 0165-7380
    ISSN (online) 1573-7446
    ISSN 0165-7380
    DOI 10.1007/s11259-023-10211-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3453: Show issues Location:
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  2. Article: First study on

    Triratapiban, Chanittha / Lueangaramkul, Varanya / Phecharat, Nantawan / Pantanam, Achiraya / Lekcharoensuk, Porntippa / Theerawatanasirikul, Sirin

    Veterinary world

    2023  Volume 16, Issue 3, Page(s) 618–630

    Abstract: Background and aim: Feline infectious peritonitis (FIP), one of the most important infectious diseases in cats is caused by FIP virus (FIPV), a mutated variant of feline coronavirus. Feline infectious peritonitis has a negative impact on feline health, ... ...

    Abstract Background and aim: Feline infectious peritonitis (FIP), one of the most important infectious diseases in cats is caused by FIP virus (FIPV), a mutated variant of feline coronavirus. Feline infectious peritonitis has a negative impact on feline health, with extremely high mortality in clinical FIP-infected cats, particularly young cats. There are no approved drugs for FIP treatment, and therapeutic possibilities for FIP treatment are limited. This study aimed to utilize nature-derived bioactive flavonoids with antiviral properties to inhibit FIPV infection in Crandell-Rees feline kidney (CRFK) cells.
    Materials and methods: The cytotoxicity of 16 flavonoids was evaluated on CRFK cells using a colorimetric method (MTS) assay. Viral kinetics of FIPV at 50 tissue culture infectious dose (TCID
    Results: Two flavonoids, namely, isoginkgetin and luteolin, inhibited FIPV replication during post-viral entry in a dose-dependent manner, with 50% maximal effective concentrations = 4.77 ± 0.09 and 36.28 ± 0.03 μM, respectively. Based on viral kinetics, both flavonoids could inhibit FIPV replication at the early stage of infection at 0-6-HPI for isoginkgetin and 2-6-HPI for luteolin using a time-of-addition assay. Isoginkgetin exerted a direct virucidal effect that reduced the viral titers by 2 and 1.89 log
    Conclusion: Isoginkgetin interfered with FIPV replication during both post-viral infection and virucidal experiments on CRFK cells, whereas luteolin inhibited the virus after infection. These results demonstrate the potential of herbal medicine for treating FIP.
    Language English
    Publishing date 2023-03-26
    Publishing country India
    Document type Journal Article
    ZDB-ID 2456277-4
    ISSN 2231-0916 ; 0972-8988
    ISSN (online) 2231-0916
    ISSN 0972-8988
    DOI 10.14202/vetworld.2023.618-630
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Small Molecules Targeting 3C Protease Inhibit FMDV Replication and Exhibit Virucidal Effect in Cell-Based Assays.

    Theerawatanasirikul, Sirin / Lueangaramkul, Varanya / Pantanam, Achiraya / Mana, Natjira / Semkum, Ploypailin / Lekcharoensuk, Porntippa

    Viruses

    2023  Volume 15, Issue 9

    Abstract: Foot-and-mouth disease (FMD) is a highly contagious disease in cloven-hoofed animals, caused by the foot-and-mouth disease virus (FMDV). It is endemic in Asia and Africa but spreads sporadically throughout the world, resulting in significant losses in ... ...

    Abstract Foot-and-mouth disease (FMD) is a highly contagious disease in cloven-hoofed animals, caused by the foot-and-mouth disease virus (FMDV). It is endemic in Asia and Africa but spreads sporadically throughout the world, resulting in significant losses in the livestock industry. Effective anti-FMDV therapeutics could be a supportive control strategy. Herein, we utilized computer-aided, structure-based virtual screening to filter lead compounds from the National Cancer Institute (NCI) diversity and mechanical libraries using FMDV 3C protease (3C
    MeSH term(s) Animals ; Peptide Hydrolases ; Foot-and-Mouth Disease Virus ; Molecular Docking Simulation ; Endopeptidases ; Antiviral Agents/pharmacology ; 3C Viral Proteases
    Chemical Substances Peptide Hydrolases (EC 3.4.-) ; Endopeptidases (EC 3.4.-) ; Antiviral Agents
    Language English
    Publishing date 2023-09-06
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v15091887
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Andrographolide and Deoxyandrographolide Inhibit Protease and IFN-Antagonist Activities of Foot-and-Mouth Disease Virus 3Cpro

    Theerawatanasirikul, Sirin / Lueangaramkul, Varanya / Thangthamniyom, Nattarat / Chankeeree, Penpitcha / Semkum, Ploypailin / Lekcharoensuk, Porntippa

    Animals. 2022 Aug. 07, v. 12, no. 15

    2022  

    Abstract: Foot-and mouth-disease (FMD) caused by the FMD virus (FMDV) is highly contagious and negatively affects livestock worldwide. The control of the disease requires a combination of measures, including vaccination; however, there is no specific treatment ... ...

    Abstract Foot-and mouth-disease (FMD) caused by the FMD virus (FMDV) is highly contagious and negatively affects livestock worldwide. The control of the disease requires a combination of measures, including vaccination; however, there is no specific treatment available. Several studies have shown that plant-derived products with antiviral properties were effective on viral diseases. Herein, antiviral activities of andrographolide (AGL), deoxyandrographolide (DAG), and neoandrographolide (NEO) against FMDV serotype A were investigated using an in vitro cell-based assay. The results showed that AGL and DAG inhibited FMDV in BHK-21 cells. The inhibitory effects of AGL and DAG were evaluated by RT-qPCR and exhibited EC50 values of 52.18 ± 0.01 µM (SI = 2.23) and 36.47 ± 0.07 µM (SI = 9.22), respectively. The intracellular protease assay revealed that AGL and DAG inhibited FMDV 3Cᵖʳᵒ with IC50 of 67.43 ± 0.81 and 25.58 ± 1.41 µM, respectively. Additionally, AGL and DAG significantly interfered with interferon (IFN) antagonist activity of the 3Cᵖʳᵒ by derepressing interferon-stimulating gene (ISGs) expression. The molecular docking confirmed that the andrographolides preferentially interacted with the 3Cᵖʳᵒ active site. However, NEO had no antiviral effect in any of the assays. Conclusively, AGL and DAG inhibited FMDV serotype A by interacting with the 3Cᵖʳᵒ and hindered its protease and IFN antagonist activities.
    Keywords Foot-and-mouth disease virus ; active sites ; andrographolide ; antagonists ; antiviral properties ; genes ; inhibitory concentration 50 ; interferons ; livestock ; median effective concentration ; proteinases ; serotypes ; vaccination
    Language English
    Dates of publication 2022-0807
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani12151995
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Non-Nucleoside Inhibitors Decrease Foot-and-Mouth Disease Virus Replication by Blocking the Viral 3D

    Theerawatanasirikul, Sirin / Semkum, Ploypailin / Lueangaramkul, Varanya / Chankeeree, Penpitcha / Thangthamniyom, Nattarat / Lekcharoensuk, Porntippa

    Viruses

    2022  Volume 15, Issue 1

    Abstract: Foot-and-mouth disease virus (FMDV), an economically important pathogen of cloven-hoofed livestock, is a positive-sense, single-stranded RNA virus classified in ... ...

    Abstract Foot-and-mouth disease virus (FMDV), an economically important pathogen of cloven-hoofed livestock, is a positive-sense, single-stranded RNA virus classified in the
    MeSH term(s) Animals ; Foot-and-Mouth Disease Virus/genetics ; Antiviral Agents/pharmacology ; Antiviral Agents/metabolism ; RNA-Dependent RNA Polymerase/metabolism ; Foot-and-Mouth Disease ; Virus Replication
    Chemical Substances Antiviral Agents ; RNA-Dependent RNA Polymerase (EC 2.7.7.48)
    Language English
    Publishing date 2022-12-30
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v15010124
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Andrographolide and Deoxyandrographolide Inhibit Protease and IFN-Antagonist Activities of Foot-and-Mouth Disease Virus 3C

    Theerawatanasirikul, Sirin / Lueangaramkul, Varanya / Thangthamniyom, Nattarat / Chankeeree, Penpitcha / Semkum, Ploypailin / Lekcharoensuk, Porntippa

    Animals : an open access journal from MDPI

    2022  Volume 12, Issue 15

    Abstract: Foot-and mouth-disease (FMD) caused by the FMD virus (FMDV) is highly contagious and negatively affects livestock worldwide. The control of the disease requires a combination of measures, including vaccination; however, there is no specific treatment ... ...

    Abstract Foot-and mouth-disease (FMD) caused by the FMD virus (FMDV) is highly contagious and negatively affects livestock worldwide. The control of the disease requires a combination of measures, including vaccination; however, there is no specific treatment available. Several studies have shown that plant-derived products with antiviral properties were effective on viral diseases. Herein, antiviral activities of andrographolide (AGL), deoxyandrographolide (DAG), and neoandrographolide (NEO) against FMDV serotype A were investigated using an in vitro cell-based assay. The results showed that AGL and DAG inhibited FMDV in BHK-21 cells. The inhibitory effects of AGL and DAG were evaluated by RT-qPCR and exhibited EC50 values of 52.18 ± 0.01 µM (SI = 2.23) and 36.47 ± 0.07 µM (SI = 9.22), respectively. The intracellular protease assay revealed that AGL and DAG inhibited FMDV 3C
    Language English
    Publishing date 2022-08-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani12151995
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Kategorien

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