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Article ; Online: Arctic Sea Ice Concentration Assimilation in an Operational Global 1/10° Ocean Forecast System

Qiuli Shao / Qi Shu / Bin Xiao / Lujun Zhang / Xunqiang Yin / Fangli Qiao

Remote Sensing, Vol 15, Iss 1274, p

2023 Volume 1274

Abstract: To understand the Arctic environment, which is closely related to sea ice and to reduce potential risks, reliable sea ice forecasts are indispensable. A practical, lightweight yet effective assimilation scheme of sea ice concentration based on Optimal ... ...

Abstract	To understand the Arctic environment, which is closely related to sea ice and to reduce potential risks, reliable sea ice forecasts are indispensable. A practical, lightweight yet effective assimilation scheme of sea ice concentration based on Optimal Interpolation is designed and adopted in an operational global 1/10° surface wave-tide-circulation coupled ocean model (FIO-COM10) forecasting system to improve Arctic sea ice forecasting. Twin numerical experiments with and without data assimilation are designed for the simulation of the year 2019, and 5-day real-time forecasts for 2021 are implemented to study the sea ice forecast ability. The results show that the large biases in the simulation and forecast of sea ice concentration are remarkably reduced due to satellite observation uncertainty levels by data assimilation, indicating the high efficiency of the data assimilation scheme. The most significant improvement occurs in the marginal ice zones. The sea surface temperature bias averaged over the marginal ice zones is also reduced by 0.9 °C. Sea ice concentration assimilation has a profound effect on improving forecasting ability. The Root Mean Square Error and Integrated Ice-Edge Error are reduced to the level of the independent satellite observation at least for 24-h forecast, and sea ice forecast by FIO-COM10 has better performance than the persistence forecasts in summer and autumn.
Keywords	sea ice concentration ; data assimilation ; global ocean forecasting system ; Science ; Q
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Adulteration Detection and Quantification in Olive Oil Using Excitation-Emission Matrix Fluorescence Spectroscopy and Chemometrics.

Lujun, Zhang / Nuo, Cai / Xiaodong, Huang / Xinmin, Fan / Juanjuan, Gao / Jin, Gao / Sensen, Li / Yan, Wang / Chunyan, Wang

Journal of fluorescence

2024

Abstract: This research investigates the use of excitation-emission matrix fluorescence (EEMF) in conjunction with chemometric models to rapidly identify and quantify adulteration in olive oil, a critical concern where sample availability is limited. Adulteration ... ...

Abstract	This research investigates the use of excitation-emission matrix fluorescence (EEMF) in conjunction with chemometric models to rapidly identify and quantify adulteration in olive oil, a critical concern where sample availability is limited. Adulteration is simulated by blending soybean, peanut, and linseed oils into olive oil, creating diverse adulterated samples. Principal component analysis (PCA) was applied to the EEMF spectral data as an initial exploratory measure to cluster and differentiate adulterated samples. Spatial clustering enabled vivid visualization of the variations and trends in the spectra. The novel application of parallel factor analysis (PARAFAC) for data decomposition in this paper focuses on unraveling correlations between the decomposed components and the actual adulterated components, which offers a novel perspective for accurately quantifying adulteration levels. Additionally, a comparative analysis was conducted between the PCA and PARAFAC methodologies. Our study not only unveils a new avenue for the quantitative analysis of adulterants in olive oil through spectral detection but also highlights the potential for applying these insights in practical, real-world scenarios, thereby enhancing detection capabilities for various edible oil samples. This promises to improve the detection of adulteration across a range of edible oil samples, offering significant contributions to food safety and quality assurance.
Language	English
Publishing date	2024-03-08
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2016892-5
ISSN	1573-4994 ; 1053-0509
ISSN (online)	1573-4994
ISSN	1053-0509
DOI	10.1007/s10895-024-03613-z
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Advantages of the latest Los Alamos Sea-Ice Model (CICE)

Huazhao WANG / Lujun ZHANG / Min CHU / Siyu HU

Atmospheric and Oceanic Science Letters, Vol 13, Iss 2, Pp 113-

evaluation of the simulated spatiotemporal variation of Arctic sea ice

2020 Volume 120

Abstract: The Los Alamos Sea-Ice Model (CICE) is one of the most popular sea-ice models. All versions of it have been the main sea-ice module coupled to climate system models. Therefore, evaluating their simulation capability is an important step in developing ... ...

Abstract	The Los Alamos Sea-Ice Model (CICE) is one of the most popular sea-ice models. All versions of it have been the main sea-ice module coupled to climate system models. Therefore, evaluating their simulation capability is an important step in developing climate system models. Compared with observations and previous versions (CICE4.0 and CICE5.0), the advantages of CICE6.0 (the latest version) are analyzed in this paper. It is found that CICE6.0 has the minimum interannual errors, and the seasonal cycle it simulates is the most consistent with observations. CICE4.0 overestimates winter sea-ice and underestimates summer sea-ice severely. Meanwhile, the errors of CICE5.0 in winter are larger than for the other versions. The main attention is paid to the perennial ice and the seasonal ice. The spatial distribution of root-mean-square errors indicates that the simulated errors are distributed in the Atlantic sector and the outer Arctic. Both CICE4.0 and CICE5.0 underestimate the concentration of the perennial ice and overestimate that of the seasonal ice in these areas. Meanwhile, CICE6.0 solves this problem commendably. Moreover, the decadal trends it simulates are comparatively the best, especially in the central Arctic sea. The other versions underestimate the decadal trend of the perennial ice and overestimate that of the seasonal ice. In addition, an index used to objectively describe the difference in the spatial distribution between the simulation and observation shows that CICE6.0 produces the best simulated spatial distribution.
Keywords	los alamos sea-ice model (cice) ; spatiotemporal variation ; perennial ice ; seasonal ice ; model evaluation ; Environmental sciences ; GE1-350 ; Oceanography ; GC1-1581
Subject code	290
Language	English
Publishing date	2020-03-01T00:00:00Z
Publisher	KeAi Communications Co., Ltd.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Identification of suitable reference genes for real-time qPCR in homocysteine-treated human umbilical vein endothelial cells.

Xia Zhu / Lujun Zhang / Yangxi Hu / Jianliang Zhang

PLoS ONE, Vol 13, Iss 12, p e

2018 Volume 0210087

Abstract: The imbalance in homocysteine (Hcy) metabolism has been implicated in the pathogenesis of human diseases, including cardiovascular and neurodegenerative disorders. When attempting to identify gene expression profiles using quantitative real-time reverse ... ...

Abstract	The imbalance in homocysteine (Hcy) metabolism has been implicated in the pathogenesis of human diseases, including cardiovascular and neurodegenerative disorders. When attempting to identify gene expression profiles using quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR), the selection of suitable reference genes is important. Here, the expression levels of 10 commonly used reference genes were assessed for normalization of RT-qPCR in Hcy-treated human umbilical vein endothelial cells (HUVECs) and control cells. The suitability of eight selected candidate genes was comparatively analyzed across the tested samples and separately ranked by four programs, geNorm, NormFinder, BestKeeper, and the ΔCt method. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was the most stable gene in the final ranking using the RankAggreg package. Surprisingly, the β-actin (ACTB) levels decreased significantly in Hcy-treated HUVECs compared with control HUVECs (P<0.05), and further study indicated that Hcy suppressed the expression of ACTB by upregulating the miR-145-5p level in Hcy-treated HUVECs. Our data suggest that GAPDH can be used as a reliable reference gene, while ACTB cannot; normalization of gene expression in RT-qPCR experiments in Hcy-treated HUVECs. The data, which identifies a suitable reference gene in Hcy-treated HUVECs, will contribute to the design of an effective and accurate method for quantitation of gene expression.
Keywords	Medicine ; R ; Science ; Q
Subject code	570
Language	English
Publishing date	2018-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Responses of the Mushroom Pleurotus ostreatus under Different CO 2 Concentration by Comparative Proteomic Analyses

Rongmei Lin / Lujun Zhang / Xiuqing Yang / Qiaozhen Li / Chenxiao Zhang / Lizhong Guo / Hao Yu / Hailong Yu

Journal of Fungi, Vol 8, Iss 7, p

2022 Volume 652

Abstract: Background: Pleurotus ostreatus is a popular edible mushroom in East Asian markets. Research on the responses of P. ostreatus under different carbon dioxide concentrations is limited. Methods: Label-free LC-MS/MS quantitative proteomics analysis ... ...

Abstract	Background: Pleurotus ostreatus is a popular edible mushroom in East Asian markets. Research on the responses of P. ostreatus under different carbon dioxide concentrations is limited. Methods: Label-free LC-MS/MS quantitative proteomics analysis technique was adopted to obtain the protein expression profiles of P. ostreatus fruiting body pileus collected under different carbon dioxide concentrations. The Pearson correlation coefficient analysis and principal component analysis were performed to reveal the correlation among samples. The differentially expressed proteins (DEPs) were organized. Gene ontology analysis was performed to divide the DEPs into different metabolic processes and pathways. Results: The expansion of stipes was inhibited in the high CO 2 group compared with that in the low CO 2 group. There were 415 DEPs (131 up- and 284 down-regulated) in P. ostreatus PH11 treated with 1% CO 2 concentration compared with P. ostreatus under atmospheric conditions. Proteins related to hydrolase activity, including several amidohydrolases and cell wall synthesis proteins, were highly expressed under high CO 2 concentration. Most of the kinases and elongation factors were significantly down-regulated under high CO 2 concentration. The results suggest that the metabolic regulation and development processes were inhibited under high CO 2 concentrations. In addition, the sexual differentiation process protein Isp4 was inhibited under high CO 2 concentrations, indicating that the sexual reproductive process was also inhibited under high CO 2 concentrations, which is inconsistent with the small fruiting body pileus under high CO 2 concentrations. Conclusions: This research reports the proteome analysis of commercially relevant edible fungi P. ostreatus under different carbon dioxide concentrations. This study deepens our understanding of the mechanism for CO 2 -induced morphological change in the P. ostreatus fruiting body, which will facilitate the artificial cultivation of edible mushrooms.
Keywords	P. ostreatus ; proteomics ; carbon dioxide ; fruiting body ; edible mushroom ; Biology (General) ; QH301-705.5
Subject code	290
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A field investigation on rill development and flow hydrodynamics under different upslope inflow and slope gradient conditions

Pei Tian / Chengzhong Pan / Xinyi Xu / Tieniu Wu / Tiantian Yang / Lujun Zhang

Hydrology Research, Vol 51, Iss 5, Pp 1201-

2020 Volume 1220

Abstract: Few studies focus on the quantitative impact of upslope inflow rate and slope gradient on rill development and erosion processes. Field plot experiments under varying inflow rates (6–36 L min−1m−1) and slope gradients (26, 42 and 57%) were conducted to ... ...

Abstract	Few studies focus on the quantitative impact of upslope inflow rate and slope gradient on rill development and erosion processes. Field plot experiments under varying inflow rates (6–36 L min−1m−1) and slope gradients (26, 42 and 57%) were conducted to address this issue. The results showed soil loss rates significantly demonstrated temporal variability in relevance to the rill developing process. Rill erosion and its contribution to soil loss increased with increasing inflow rates and slope gradients by power functions. There was a threshold inflow discharge (12–24 L min−1m−1), under which, rill erosion became the dominant erosion pattern. At the initial stage, downcutting of rill bottom and headward erosion were obvious, whereas rill broadening was significant at the actively rill developing period. Rill density increased with slope gradient increasing from 26% to 42%, and then decreased. For the 57% slope under high inflow rates (24–36 L min−1m−1), gravity caused an increase in the collapse of rills. Mean rill width increased with increasing inflow rates but decreased as slope gradients increased, while mean rill depth increased with increasing inflow rates and slope gradients. Stream power and rill flow velocity were the best hydrodynamic parameter to simulate rill erosion and rill morphology, respectively. HIGHLIGHTS Rill erosion and its contribution to soil loss increased with inflow rate and slope gradient by a power function.; The threshold inflow rate that made rill erosion dominated decreased with increasing slope gradient.; There was a threshold slope gradient between 42.3% and 57.4% where the rill network development began to weaken.; Stream power was the best parameter simulating rill erosion with a good linear relationship.;
Keywords	flow hydrodynamics ; rill development ; rill morphology ; slope gradient ; upslope inflow rate ; River ; lake ; and water-supply engineering (General) ; TC401-506 ; Physical geography ; GB3-5030
Subject code	669
Language	English
Publishing date	2020-10-01T00:00:00Z
Publisher	IWA Publishing
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Study on Adsorption Performance of Benzoic Acid in Cyclocarya paliurus Extract by Ethyl Cellulose Microspheres

Yamin Zhao / Lujun Zhang / Xiaoxue Zhai / Qian Liu / Lebing Sun / Mengshi Liu / Lili An / Liang Xian / Ping Zhang / Lihua Chen

Chemistry, Vol 3, Iss 81, Pp 1113-

2021 Volume 1125

Abstract: Polymer microspheres with inter-connecting pores are widely used as microsphere materials. In the study, the ethyl cellulose microspheres (ECM) were prepared by using the solvent-evaporation method. Based on that, a method for the separation and ... ...

Abstract	Polymer microspheres with inter-connecting pores are widely used as microsphere materials. In the study, the ethyl cellulose microspheres (ECM) were prepared by using the solvent-evaporation method. Based on that, a method for the separation and purification of benzoic acid from crude extract of Cyclocarya paliurus was established by the ECM and high performance liquid chromatography (HPLC). The ECM after the sorption equilibrium was desorbed by using 40% methanol as the analytical solvent. The content of benzoic acid in eluent is up to 0.0216 mg/mL, and the benzoic acid can be obtained with a high purity of 82.22%. Furthermore, the adsorption-desorption behavior of benzoic acid onto ECM was investigated. The results of adsorption kinetics of benzoic acid showed that the adsorption followed the pseudo-first-order kinetic model. The ECM was characterized by using Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning electron microscope (SEM), and X-ray diffraction (XRD). The results showed that the ECM has a high adsorption property due to its more porous structure, phenolic hydroxyl group, and other oxygen-containing functional groups. This method and the ECM can be used stably, continuously, and efficiently to purify the benzoic acid from the methanol extract of C. paliurus on a large scale.
Keywords	ethyl cellulose microspheres (ECM) ; HPLC ; adsorption-desorption ; benzoic acid ; Cyclocarya paliurus ; Chemistry ; QD1-999
Subject code	540 ; 660
Language	English
Publishing date	2021-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: GMPR

Li Chen / James Reeve / Lujun Zhang / Shengbing Huang / Xuefeng Wang / Jun Chen

PeerJ, Vol 6, p e

A robust normalization method for zero-inflated count data with application to microbiome sequencing data

2018 Volume 4600

Abstract: Normalization is the first critical step in microbiome sequencing data analysis used to account for variable library sizes. Current RNA-Seq based normalization methods that have been adapted for microbiome data fail to consider the unique characteristics ...

Abstract	Normalization is the first critical step in microbiome sequencing data analysis used to account for variable library sizes. Current RNA-Seq based normalization methods that have been adapted for microbiome data fail to consider the unique characteristics of microbiome data, which contain a vast number of zeros due to the physical absence or under-sampling of the microbes. Normalization methods that specifically address the zero-inflation remain largely undeveloped. Here we propose geometric mean of pairwise ratios—a simple but effective normalization method—for zero-inflated sequencing data such as microbiome data. Simulation studies and real datasets analyses demonstrate that the proposed method is more robust than competing methods, leading to more powerful detection of differentially abundant taxa and higher reproducibility of the relative abundances of taxa.
Keywords	Normalization ; Metagenomics ; Microbiome ; Statistics ; Zero-inflation ; RNA-seq ; Medicine ; R ; Biology (General) ; QH301-705.5
Subject code	310
Language	English
Publishing date	2018-04-01T00:00:00Z
Publisher	PeerJ Inc.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Structural Characterization and Evolutionary Relationship of High-Molecular-Weight Glutenin Subunit Genes in Roegneria nakaii and Roegneria alashanica

Lujun Zhang / Zhixin Li / Renchun Fan / Bo Wei / Xiangqi Zhang

International Journal of Molecular Sciences, Vol 17, Iss 7, p

2016 Volume 1115

Abstract: The Roegneria of Triticeae is a large genus including about 130 allopolyploid species. Little is known about its high-molecular-weight glutenin subunits (HMW-GSs). Here, we reported six novel HMW-GS genes from R. nakaii and R. alashanica. Sequencing ... ...

Abstract	The Roegneria of Triticeae is a large genus including about 130 allopolyploid species. Little is known about its high-molecular-weight glutenin subunits (HMW-GSs). Here, we reported six novel HMW-GS genes from R. nakaii and R. alashanica. Sequencing indicated that Rny1, Rny3, and Ray1 possessed intact open reading frames (ORFs), whereas Rny2, Rny4, and Ray2 harbored in-frame stop codons. All of the six genes possessed a similar primary structure to known HMW-GS, while showing some unique characteristics. Their coding regions were significantly shorter than Glu-1 genes in wheat. The amino acid sequences revealed that all of the six genes were intermediate towards the y-type. The phylogenetic analysis showed that the HMW-GSs from species with St, StY, or StH genome(s) clustered in an independent clade, varying from the typical x- and y-type clusters. Thus, the Glu-1 locus in R. nakaii and R. alashanica is a very primitive glutenin locus across evolution. The six genes were phylogenetically split into two groups clustered to different clades, respectively, each of the two clades included the HMW-GSs from species with St (diploid and tetraploid species), StY, and StH genomes. Hence, it is concluded that the six Roegneria HMW-GS genes are from two St genomes undergoing slight differentiation.
Keywords	Roegneria nakaii ; Roegneria alashanica ; high-molecular-weight glutenin subunits ; gene cloning ; variation ; phylogenetic analysis ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	590
Language	English
Publishing date	2016-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Driving dimethyl carbonate synthesis from CO2 and methanol and production of acetylene simultaneously using CaC2

Zhang, Zhaofu / Buxing Han / Guanying Yang / Lujun Zhang / Shuai Yin / Shuaishuai Liu

Chemical communications. 2018 Apr. 26, v. 54, no. 35

2018

Abstract: The synthesis of dimethyl carbonate (DMC) from CO2 and methanol is a very interesting reaction, but is thermodynamically limited. In this work, CaC2 was used to consume the water produced in the reaction to shift the reaction equilibrium, and C2H2 was ... ...

Abstract	The synthesis of dimethyl carbonate (DMC) from CO2 and methanol is a very interesting reaction, but is thermodynamically limited. In this work, CaC2 was used to consume the water produced in the reaction to shift the reaction equilibrium, and C2H2 was produced at the same time. This is the first work on the combination of driving a thermodynamically unfavorable reaction and producing C2H2 using CaC2.
Keywords	acetylene ; calcium carbide ; carbon dioxide ; carbonates ; chemical reactions ; methanol ; thermodynamics
Language	English
Dates of publication	2018-0426
Size	p. 4410-4412.
Publishing place	The Royal Society of Chemistry
Document type	Article
ZDB-ID	1472881-3
ISSN	1364-548X ; 1359-7345 ; 0009-241X
ISSN (online)	1364-548X
ISSN	1359-7345 ; 0009-241X
DOI	10.1039/c8cc01005f
Database	NAL-Catalogue (AGRICOLA)

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