Article ; Online: Single-cell atlas of early chick development reveals gradual segregation of neural crest lineage from the neural plate border during neurulation.
2022 Volume 11
Abstract: The epiblast of vertebrate embryos is comprised of neural and non-neural ectoderm, with the border territory at their intersection harboring neural crest and cranial placode progenitors. Here, we a generate single-cell atlas of the developing chick ... ...
| Abstract | The epiblast of vertebrate embryos is comprised of neural and non-neural ectoderm, with the border territory at their intersection harboring neural crest and cranial placode progenitors. Here, we a generate single-cell atlas of the developing chick epiblast from late gastrulation through early neurulation stages to define transcriptional changes in the emerging 'neural plate border' as well as other regions of the epiblast. Focusing on the border territory, the results reveal gradual establishment of heterogeneous neural plate border signatures, including novel genes that we validate by fluorescent in situ hybridization. Developmental trajectory analysis infers that segregation of neural plate border lineages only commences at early neurulation, rather than at gastrulation as previously predicted. We find that cells expressing the prospective neural crest marker |
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| MeSH term(s) | Animals ; Chick Embryo/cytology ; Chickens/physiology ; Gene Expression Regulation, Developmental ; Germ Layers/physiology ; In Situ Hybridization, Fluorescence ; Neural Crest/embryology ; Neural Plate/embryology ; Neurulation/physiology ; PAX7 Transcription Factor/analysis |
| Chemical Substances | PAX7 Transcription Factor |
| Language | English |
| Publishing date | 2022-01-28 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2687154-3 |
| ISSN | 2050-084X ; 2050-084X |
| ISSN (online) | 2050-084X |
| ISSN | 2050-084X |
| DOI | 10.7554/eLife.74464 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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