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Article ; Online: Analysis of circular RNA expression profile of pathological bone formation in ankylosing spondylitis.

Zou, Yu-Cong / Wu, Juan / Zhao, Chang / Luo, Zi-Rui

International journal of rheumatic diseases

2023 Volume 26, Issue 7, Page(s) 1403–1406

Abstract: Objective: To screen and analyze the function of specific CircRNAs involved in pathological bone formation in patients with ankylosing spondylitis (AS).: Methods: From September 2019 to October 2020, hip capsule tissues obtained from 3 patients with ... ...

Abstract	Objective: To screen and analyze the function of specific CircRNAs involved in pathological bone formation in patients with ankylosing spondylitis (AS). Methods: From September 2019 to October 2020, hip capsule tissues obtained from 3 patients with AS developed hip joint fusion and 3 patients with femoral neck fracture (FNF) were obtained. The circular RNA expressions of hip capsule were analyzed by Arraystar CircRNA chip. qRT-PCR analysis wan performed to identify the expression patterns of differentially expression CircRNAs. Results: Our findings showed that there were 25 up-regulated and 39 down-regulated differential CircRNAs. Among these CircRNAs, we screened 10 highest up-regulatedCircRNAs and 13 lowest down-regulated CircRNAs (Fold Change≥2,P<0.05). In further verification analysis, hsa_circ_0067103, hsa_circ_0004496, and hsa_circ_0002649, ACTG1 were significantly upregulated, while hsa_circ_0020273, hsa_circ_0005699, and hsa_circ_0048764 were markly downregulated in AS tissue than FNF controls. Conclusion: The expression of CircRNAs involved of pathological bone formation in AS were significantly different from those of control group. These differentially expressed Circular RNAs may be closely related to the occurrence and development of pathological bone formation in AS.
MeSH term(s)	Humans ; RNA, Circular/genetics ; Spondylitis, Ankylosing/genetics ; Osteogenesis/genetics ; Oligonucleotide Array Sequence Analysis
Chemical Substances	RNA, Circular
Language	English
Publishing date	2023-03-06
Publishing country	England
Document type	Letter
ZDB-ID	2426924-4
ISSN	1756-185X ; 1756-1841
ISSN (online)	1756-185X
ISSN	1756-1841
DOI	10.1111/1756-185X.14638
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Article ; Online: DNA methylation of DKK-1 may correlate with pathological bone formation in ankylosing spondylitis.

Zou, Yu-Cong / Wang, Zhi-Jun / Shao, Li-Cheng / Xia, Zhi-Hong / Lan, Yi-Feng / Yu, Zhi-Hui / Yao, Jia-Yu / Luo, Zi-Rui

Immunity, inflammation and disease

2023 Volume 11, Issue 7, Page(s) e911

Abstract: Objective: To investigate DNA methylation (DNAm) status of dickkopf-associated protein 1 (DKK-1) in ossified hip capsule synovium and serum among patients with ankylosing spondylitis (AS).: Methods: Western blot was applied to detect the level of DKK- ...

Abstract	Objective: To investigate DNA methylation (DNAm) status of dickkopf-associated protein 1 (DKK-1) in ossified hip capsule synovium and serum among patients with ankylosing spondylitis (AS). Methods: Western blot was applied to detect the level of DKK-1 protein expression in hip joint capsule tissues from four patients with AS as well as four patients with femoral neck fracture (FNF) caused by trauma as control. DKK-1 gene promoter methylation (GPM) was examined by methylation-specific polymerase chain reaction. Reverse transcription-polymerase chain reaction was performed to examine the messenger RNA (mRNA) levels of DKK-1, β-catenin, and Wnt3a in both tissue and serum. The DNAm status of serum DKK-1 was measured among 36 patients with AS and syndesmophytes (AS + syndesmophytes group), 40 patients with AS but no syndesmophyte (AS group), and 42 healthy individuals (control group). Also, the serum levels of DKK-1 were measured by enzyme-linked immunosorbent assay. The modified New York criteria (mNYC) together with the modified Stoke Ankylosing Spondylitis Spinal Score (mSASSS) were adopted to examine the radiographic progression of AS. The receiver operating characteristic (ROC) curve was applied to investigate the diagnostic value of the methylation rate of DKK-1 with regard to radiographic progression. Results: The expressions of DKK-1 protein and mRNA in hip joint capsule tissues of AS patients were significantly lower, while DKK-1 GPM rate, β-catenin mRNA, and Wnt3a mRNA were markedly higher when compared with FNF group. For serum samples, the DKK-1 methylation rate was significantly higher in AS+ syndesmophytes group in contrast to AS group and healthy controls. Serum levels of DKK-1 protein and mRNA in AS with syndesmophytes group were markedly decreased, while β-catenin mRNA and Wnt3a mRNA expressions were significantly increased than AS with no syndesmophyte group and the healthy control group. AS patients in Grade 4 showed a significantly higher serum DKK-1 GPM rate than those in Grade 3 based on mNYC. Serum DKK-1 GPM level was markedly and positively correlated with mSASSS. Serum levels of DKK-1 in AS+ syndesmophytes group were markedly lower compared with AS but no syndesmophyte group and healthy controls. ROC curve analysis indicated that serum DKK-1 methylation rate serves as a decent indicator for AS radiographic progression. Conclusion: DNAm of DKK-1 may correlate with pathological bone formation in AS, which may provide new strategies for the treatment of AS abnormal bone formation.
MeSH term(s)	Humans ; beta Catenin/genetics ; DNA Methylation ; Osteogenesis ; Spine/pathology ; Spondylitis, Ankylosing/genetics
Chemical Substances	beta Catenin ; DKK1 protein, human
Language	English
Publishing date	2023-07-28
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2740382-8
ISSN	2050-4527 ; 2050-4527
ISSN (online)	2050-4527
ISSN	2050-4527
DOI	10.1002/iid3.911
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Anti-phase pulsation of counter-propagating dissipative solitons in a bidirectional fiber laser.

Yang, Kai / Luo, Zi-Rui / Zhang, Ze-Xian / Zhan, Ze-Yu / Wu, Dai-Xuan / Liu, Meng / Luo, Ai-Ping / Xu, Wen-Cheng / Luo, Zhi-Chao

Optics letters

2023 Volume 48, Issue 24, Page(s) 6464–6467

Abstract: Due to its unique geometric structure, the bidirectional ultrafast fiber laser is an excellent light source for dual-comb applications. However, sharing the same gain between the counter-propagating solitons also gives rise to complex dynamics. Herein, ... ...

Abstract	Due to its unique geometric structure, the bidirectional ultrafast fiber laser is an excellent light source for dual-comb applications. However, sharing the same gain between the counter-propagating solitons also gives rise to complex dynamics. Herein, we report the anti-phase pulsation of counter-propagating dissipative solitons in a bidirectional fiber laser. The in-phase and anti-phase soliton pulsation can be manipulated by adjusting the intracavity birefringence. The periodic modulation of polarization-dependent gain (PDG) caused by polarization hole burning (PHB) in the gain fiber can be responsible for anti-phase pulsation of bidirectional dissipative solitons. These findings offer new, to the best of our knowledge, insights into the complex dynamics of solitons in dissipative optical systems and performance improvement of bidirectional ultrafast fiber lasers.
Language	English
Publishing date	2023-12-15
Publishing country	United States
Document type	Journal Article
ISSN	1539-4794
ISSN (online)	1539-4794
DOI	10.1364/OL.502534
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Article ; Online: Pi-Pa-Run-Fei-Tang alleviates lung injury by modulating IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB signaling pathway and balancing Th17 and Treg in murine model of OVA-induced asthma.

Jie, Xiao-Lu / Luo, Zi-Rui / Yu, Jin / Tong, Zhe-Ren / Li, Qiao-Qiao / Wu, Jia-Hui / Tao, Yi / Feng, Pei-Shi / Lan, Ji-Ping / Wang, Ping

Journal of ethnopharmacology

2023 Volume 317, Page(s) 116719

Abstract: Ethnopharmacological relevance: Pi-Pa-Run-Fei-Tang (PPRFT) is an empirical TCM prescription for treating asthma. However, the underlying mechanisms of PPRFT in asthma treatment have yet to be elucidated. Recent advances have revealed that some natural ... ...

Abstract	Ethnopharmacological relevance: Pi-Pa-Run-Fei-Tang (PPRFT) is an empirical TCM prescription for treating asthma. However, the underlying mechanisms of PPRFT in asthma treatment have yet to be elucidated. Recent advances have revealed that some natural components could ameliorate asthma injury by affecting host metabolism. Untargeted metabolomics can be used to better understand the biological mechanisms underlying asthma development and identify early biomarkers that can help advance treatment. Aim of the study: The aim of this study was to verification the efficacy of PPRFT in the treatment of asthma and to preliminarily explore its mechanism. Materials and methods: A mouse asthma model was built by OVA induction. Inflammatory cell in BALF was counted. The level of IL-6, IL-1β, and TNF-α in BALF were measured. The levels of IgE in the serum and EPO, NO, SOD, GSH-Px, and MDA in the lung tissue were measured. Furthermore, pathological damage to the lung tissues was detected to evaluate the protective effects of PPRFT. The serum metabolomic profiles of PPRFT in asthmatic mice were determined by GC-MS. The regulatory effects on mechanism pathways of PPRFT in asthmatic mice were explored via immunohistochemical staining and western blotting analysis. Results: PPRFT displayed lung-protective effects through decreasing oxidative stress, airway inflammation, and lung tissue damage in OVA-induced mice, which was demonstrated by decreasing inflammatory cell levels, IL-6, IL-1β, and TNF-α levels in BALF, and IgE levels in serum, decreasing EPO, NO, and MDA levels in lung tissue, elevating SOD and GSH-Px levels in lung tissue and lung histopathological changes. In addition, PPRFT could regulate the imbalance in Th17/Treg cell ratios, suppress RORγt, and increase the expression of IL-10 and Foxp3 in the lung. Moreover, PPRFT treatment led to decreased expression of IL-6, p-JAK2/Jak2, p-STAT3/STAT3, IL-17, NF-κB, p-AKT/AKT, and p-PI3K/PI3K. Serum metabolomics analysis revealed that 35 metabolites were significantly different among different groups. Pathway enrichment analysis indicated that 31 pathways were involved. Moreover, correlation analysis and metabolic pathway analysis identified three key metabolic pathways: galactose metabolism; tricarboxylic acid cycle; and glycine, serine, and threonine metabolism. Conclusion: This research indicated that PPRFT treatment not only attenuates the clinical symptoms of asthma but is also involved in regulating serum metabolism. The anti-asthmatic activity of PPRFT may be associated with the regulatory effects of IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB mechanistic pathways.
MeSH term(s)	Mice ; Animals ; NF-kappa B/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Ovalbumin/toxicity ; Interleukin-6/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Lung Injury/chemically induced ; Lung Injury/drug therapy ; Lung Injury/metabolism ; Tumor Necrosis Factor-alpha/metabolism ; Interleukin-17/metabolism ; T-Lymphocytes, Regulatory ; Disease Models, Animal ; Cytokines/metabolism ; Asthma/chemically induced ; Asthma/drug therapy ; Asthma/metabolism ; Signal Transduction ; Lung ; Immunoglobulin E ; Superoxide Dismutase/metabolism ; Mice, Inbred BALB C
Chemical Substances	NF-kappa B ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Ovalbumin (9006-59-1) ; Interleukin-6 ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Tumor Necrosis Factor-alpha ; Interleukin-17 ; Cytokines ; Immunoglobulin E (37341-29-0) ; Superoxide Dismutase (EC 1.15.1.1)
Language	English
Publishing date	2023-05-31
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116719
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Article ; Online: Pi-Pa-Run-Fei-Tang alleviates lung injury by modulating IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB signaling pathway and balancing Th17 and Treg in murine model of OVA-induced asthma

Jie, Xiao-Lu / Luo, Zi-Rui / Yu, Jin / Tong, Zhe-Ren / Li, Qiao-Qiao / Wu, Jia-Hui / Tao, Yi / Feng, Pei-Shi / Lan, Ji-Ping / Wang, Ping

Journal of Ethnopharmacology. 2023 Dec., v. 317 p.116719-

2023

Abstract: Pi-Pa-Run-Fei-Tang (PPRFT) is an empirical TCM prescription for treating asthma. However, the underlying mechanisms of PPRFT in asthma treatment have yet to be elucidated. Recent advances have revealed that some natural components could ameliorate asthma ...

Abstract	Pi-Pa-Run-Fei-Tang (PPRFT) is an empirical TCM prescription for treating asthma. However, the underlying mechanisms of PPRFT in asthma treatment have yet to be elucidated. Recent advances have revealed that some natural components could ameliorate asthma injury by affecting host metabolism. Untargeted metabolomics can be used to better understand the biological mechanisms underlying asthma development and identify early biomarkers that can help advance treatment. The aim of this study was to verification the efficacy of PPRFT in the treatment of asthma and to preliminarily explore its mechanism. A mouse asthma model was built by OVA induction. Inflammatory cell in BALF was counted. The level of IL-6, IL-1β, and TNF-α in BALF were measured. The levels of IgE in the serum and EPO, NO, SOD, GSH-Px, and MDA in the lung tissue were measured. Furthermore, pathological damage to the lung tissues was detected to evaluate the protective effects of PPRFT. The serum metabolomic profiles of PPRFT in asthmatic mice were determined by GC-MS. The regulatory effects on mechanism pathways of PPRFT in asthmatic mice were explored via immunohistochemical staining and western blotting analysis. PPRFT displayed lung-protective effects through decreasing oxidative stress, airway inflammation, and lung tissue damage in OVA-induced mice, which was demonstrated by decreasing inflammatory cell levels, IL-6, IL-1β, and TNF-α levels in BALF, and IgE levels in serum, decreasing EPO, NO, and MDA levels in lung tissue, elevating SOD and GSH-Px levels in lung tissue and lung histopathological changes. In addition, PPRFT could regulate the imbalance in Th17/Treg cell ratios, suppress RORγt, and increase the expression of IL-10 and Foxp3 in the lung. Moreover, PPRFT treatment led to decreased expression of IL-6, p-JAK2/Jak2, p-STAT3/STAT3, IL-17, NF-κB, p-AKT/AKT, and p-PI3K/PI3K. Serum metabolomics analysis revealed that 35 metabolites were significantly different among different groups. Pathway enrichment analysis indicated that 31 pathways were involved. Moreover, correlation analysis and metabolic pathway analysis identified three key metabolic pathways: galactose metabolism; tricarboxylic acid cycle; and glycine, serine, and threonine metabolism. This research indicated that PPRFT treatment not only attenuates the clinical symptoms of asthma but is also involved in regulating serum metabolism. The anti-asthmatic activity of PPRFT may be associated with the regulatory effects of IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB mechanistic pathways.
Keywords	animal models ; asthma ; biomarkers ; blood serum ; galactose ; histopathology ; immunohistochemistry ; inflammation ; interleukin-10 ; interleukin-17 ; interleukin-6 ; lungs ; metabolism ; metabolites ; metabolomics ; mice ; oxidative stress ; serine ; threonine ; traditional medicine ; tricarboxylic acid cycle ; PPRFT ; Th17/Treg
Language	English
Dates of publication	2023-12
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116719
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Neonicotinoid insecticides promote breast cancer progression via G protein-coupled estrogen receptor: In vivo, in vitro and in silico studies.

Li, Xin / He, Sen / Xiao, Han / He, Ting-Ting / Zhang, Jia-Da / Luo, Zi-Rui / Ma, Jie-Zhi / Yin, Yu-Long / Luo, Lin / Cao, Lin-Ying

Environment international

2022 Volume 170, Page(s) 107568

Abstract: Neonicotinoid insecticides (NIs) have been widely detected in environmental media and human body with concentrations reaching hundreds of nanomolar to micromolar levels. However, the information about their human health toxicology and mechanism is ... ...

Abstract	Neonicotinoid insecticides (NIs) have been widely detected in environmental media and human body with concentrations reaching hundreds of nanomolar to micromolar levels. However, the information about their human health toxicology and mechanism is deficient. Previous studies have implied that NIs might exert estrogenic disruption and promote breast cancer progression, but the molecular mechanism is unclear, especially the molecular initiating event. G protein-coupled estrogen receptor (GPER), as a candidate therapeutic target, plays vital roles in the development of breast cancer. This work aimed to reveal the potential mechanism through GPER pathway. Firstly, we screened the activities of seven most common NIs on GPER signal pathway by calcium mobilization assay. Clothianidin, acetamiprid (ACE), and dinotefuran activated GPER most potently and ACE displayed the highest agonistic activity with the lowest observed effective concentration (LOEC) of 1 μM. The molecular docking and dynamics simulation showed favored interaction trend between the NIs and GPER. The three NIs with GPER activity induced 4T1 breast cancer cells migration and ACE showed the highest potency with LOEC of 100 nM. ACE also induced 4T1 cells proliferation at high concentration of 50 μM and up-regulated GPER expression in a dose-dependent manner. We speculated that both the induction effects of ACE on 4T1 cells proliferation and migration might be owing to the activation and up-regulation of GPER. By using 4T1-Luc cells injected orthotopic tumor model, we found that ACE also promoted in-situ breast cancer growth and lung metastasis in normal mouse dependent on GPER. However, ACE only promoted in-situ breast cancer growth through GPER but not lung metastasis in ovariectomized mice, implying that the ACE-induced lung metastasis should be related to endogenous estrogen from ovary. Overall, we demonstrated that NIs promoted breast cancer progression via GPER pathway at human related exposure levels and their female health risks need urgent concerns.
MeSH term(s)	Humans ; Female ; Mice ; Animals ; Receptors, Estrogen ; Molecular Docking Simulation ; Neoplasms ; Estrogens ; GTP-Binding Proteins
Chemical Substances	Receptors, Estrogen ; Estrogens ; GTP-Binding Proteins (EC 3.6.1.-)
Language	English
Publishing date	2022-10-06
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	554791-x
ISSN	1873-6750 ; 0160-4120
ISSN (online)	1873-6750
ISSN	0160-4120
DOI	10.1016/j.envint.2022.107568
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Article ; Online: Analysis of circular RNA expression profile of pathological bone formation in ankylosing spondylitis.

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Article ; Online: DNA methylation of DKK-1 may correlate with pathological bone formation in ankylosing spondylitis.

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Article ; Online: Anti-phase pulsation of counter-propagating dissipative solitons in a bidirectional fiber laser.

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Article ; Online: Pi-Pa-Run-Fei-Tang alleviates lung injury by modulating IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB signaling pathway and balancing Th17 and Treg in murine model of OVA-induced asthma.

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Article ; Online: Pi-Pa-Run-Fei-Tang alleviates lung injury by modulating IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB signaling pathway and balancing Th17 and Treg in murine model of OVA-induced asthma

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Article ; Online: Neonicotinoid insecticides promote breast cancer progression via G protein-coupled estrogen receptor: In vivo, in vitro and in silico studies.

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