Article ; Online: Generation and characterization of infectious molecular clones of transmitted/founder HIV-1 subtype C viruses.
2023 Volume 583, Page(s) 14–26
Abstract: The genetic diversity of HIV impedes vaccine development. Identifying the viral properties of transmitted/founder (T/F) variants may provide a common vaccine target. To study the biological nature of T/F viruses, we constructed full-length clones from ... ...
| Abstract | The genetic diversity of HIV impedes vaccine development. Identifying the viral properties of transmitted/founder (T/F) variants may provide a common vaccine target. To study the biological nature of T/F viruses, we constructed full-length clones from women detected during Fiebig stage I acute HIV-1 infection (AHI) from heterosexual male-to-female (MTF) transmission; and clones after one year of infection using In-Fusion-based cloning. Eighteen full-length T/F clones were generated from 9 women and six chronic infection clones were from 2 individuals. All clones but one were non-recombinant subtype C. Three of the 5 T/F clones and 3 chronic clones tested replicated efficiently in PBMCs and utilised CCR5 coreceptor for cell entry. Transmitted/founder and chronic infection clones displayed heterogenous in vitro replicative capacity and resistance to type I interferon. T/F viruses had shorter Env glycoproteins and fewer N-linked glycosylation sites in Env. Our findings suggest MTF transmission may select viruses with compact envelopes. |
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| MeSH term(s) | Humans ; Male ; Female ; HIV-1 ; Persistent Infection ; HIV Infections ; Clone Cells |
| Language | English |
| Publishing date | 2023-04-10 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 200425-2 |
| ISSN | 1096-0341 ; 0042-6822 |
| ISSN (online) | 1096-0341 |
| ISSN | 0042-6822 |
| DOI | 10.1016/j.virol.2023.04.001 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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