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  1. Article: A novel signature combing cuproptosis- and ferroptosis-related genes in sepsis-induced cardiomyopathy.

    Song, Juanjuan / Ren, Kairui / Zhang, Dexin / Lv, Xinpeng / Sun, Lin / Deng, Ying / Zhu, Huadong

    Frontiers in genetics

    2023  Volume 14, Page(s) 1170737

    Abstract: Objective: ...

    Abstract Objective:
    Language English
    Publishing date 2023-03-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606823-0
    ISSN 1664-8021
    ISSN 1664-8021
    DOI 10.3389/fgene.2023.1170737
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Label-free detection of virus based on surface-enhanced Raman scattering.

    Lv, Xinpeng / Zhang, Zhe / Zhao, Yue / Sun, Xiaomeng / Jiang, Heng / Zhang, Shuwen / Sun, Xianqi / Qiu, Xiaohong / Li, Yang

    Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

    2023  Volume 302, Page(s) 123087

    Abstract: Due to the background interference from biological samples, detecting viruses using surface-enhanced Raman scattering (SERS) in clinical samples is challenging. This study is based on SERS by reducing sodium borohydride and aggregating silver ... ...

    Abstract Due to the background interference from biological samples, detecting viruses using surface-enhanced Raman scattering (SERS) in clinical samples is challenging. This study is based on SERS by reducing sodium borohydride and aggregating silver nanoparticles to develop suitable virus detection "hot spot." The monkeypox virus and human papillomavirus fingerprints were quickly obtained, tested, and identified in serum and artificial vaginal discharge, respectively, by combining the principal component analysis method. Therefore, these viruses were successfully identified in the biological background. In addition, the lowest detection limit was 100 copies/mL showing good reproducibility and signal-to-noise ratio. The concentration-dependent curve of the monkeypox virus had a good linear relationship. This method helps solve the SERS signal interference problem in complex biological samples, with low detection limits and high selectivity in virus characterization and quantitative analysis. Therefore, this method has a reasonable prospect of clinical application.
    MeSH term(s) Humans ; Spectrum Analysis, Raman/methods ; Metal Nanoparticles ; Reproducibility of Results ; Silver ; Viruses ; Limit of Detection
    Chemical Substances Silver (3M4G523W1G)
    Language English
    Publishing date 2023-06-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 210413-1
    ISSN 1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
    ISSN (online) 1873-3557
    ISSN 0370-8322 ; 0584-8539 ; 1386-1425
    DOI 10.1016/j.saa.2023.123087
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Universal Method for Label-Free Detection of Pathogens and Biomolecules by Surface-Enhanced Raman Spectroscopy Based on Gold Nanoparticles

    Liu, Ling / Zhang, Ting / Wu, Zheng / Zhang, Fenghai / Wang, Yunpeng / Wang, Xiaotong / Zhang, Zhe / Li, Chengming / Lv, Xinpeng / Chen, Deqiang / Jiao, Songyan / Wu, Jing / Li, Yang

    Analytical Chemistry. 2023 Feb. 13, v. 95, no. 8 p.4050-4058

    2023  

    Abstract: The detection of biomolecules is the key to basic molecular research, diagnostics, drug screening, and other biomedical applications. However, the existing detection techniques can only detect single classes of biomolecules, which warrant the development ...

    Abstract The detection of biomolecules is the key to basic molecular research, diagnostics, drug screening, and other biomedical applications. However, the existing detection techniques can only detect single classes of biomolecules, which warrant the development of a versatile biomolecule detection platform. Here, we developed a universal method for label-free detection of biomolecules via surface-enhanced Raman spectroscopy (SERS) by using sulfhydryl-modified gold nanoparticles as the substrate. The biomolecules can be adsorbed on the surface of gold nanoparticles cleaned by bromide ions to obtain initially enhanced Raman signals, and the aggregator (calcium ion) was further added to form a “hot spot”, which enhanced the biomolecular signal again. Through the “two-step enhancement method”, we were able to obtain fingerprints of DNA, RNA, amino acids, peptides, proteins, viruses, bacteria, and lipid molecules. This low-toxic, highly sensitive, and widely applicable technique has potential applications in biomedical research, clinical testing, and disease diagnosis and lays the foundation for the development of SERS technology in various fields.
    Keywords DNA ; RNA ; Raman spectroscopy ; analytical chemistry ; calcium ; diagnostic techniques ; drugs ; lipids ; nanogold ; peptides
    Language English
    Dates of publication 2023-0213
    Size p. 4050-4058.
    Publishing place American Chemical Society
    Document type Article ; Online
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c04525
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Universal Method for Label-Free Detection of Pathogens and Biomolecules by Surface-Enhanced Raman Spectroscopy Based on Gold Nanoparticles.

    Liu, Ling / Zhang, Ting / Wu, Zheng / Zhang, Fenghai / Wang, Yunpeng / Wang, Xiaotong / Zhang, Zhe / Li, Chengming / Lv, Xinpeng / Chen, Deqiang / Jiao, Songyan / Wu, Jing / Li, Yang

    Analytical chemistry

    2023  Volume 95, Issue 8, Page(s) 4050–4058

    Abstract: The detection of biomolecules is the key to basic molecular research, diagnostics, drug screening, and other biomedical applications. However, the existing detection techniques can only detect single classes of biomolecules, which warrant the development ...

    Abstract The detection of biomolecules is the key to basic molecular research, diagnostics, drug screening, and other biomedical applications. However, the existing detection techniques can only detect single classes of biomolecules, which warrant the development of a versatile biomolecule detection platform. Here, we developed a universal method for label-free detection of biomolecules via surface-enhanced Raman spectroscopy (SERS) by using sulfhydryl-modified gold nanoparticles as the substrate. The biomolecules can be adsorbed on the surface of gold nanoparticles cleaned by bromide ions to obtain initially enhanced Raman signals, and the aggregator (calcium ion) was further added to form a "hot spot", which enhanced the biomolecular signal again. Through the "two-step enhancement method", we were able to obtain fingerprints of DNA, RNA, amino acids, peptides, proteins, viruses, bacteria, and lipid molecules. This low-toxic, highly sensitive, and widely applicable technique has potential applications in biomedical research, clinical testing, and disease diagnosis and lays the foundation for the development of SERS technology in various fields.
    MeSH term(s) Gold/chemistry ; Spectrum Analysis, Raman/methods ; Metal Nanoparticles/chemistry ; Proteins ; DNA
    Chemical Substances Gold (7440-57-5) ; Proteins ; DNA (9007-49-2)
    Language English
    Publishing date 2023-02-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c04525
    Database MEDical Literature Analysis and Retrieval System OnLINE

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