Article: Identification and characterization of GIP1, an Arabidopsis thaliana protein that enhances the DNA binding affinity and reduces the oligomeric state of G-box binding factors.
2005 Volume 15, Issue 8, Page(s) 567–575
Abstract: Environmental control of the alcohol dehydrogenase (Adh) and other stress response genes in plants is in part brought about by transcriptional regulation involving the G-box cis-acting DNA element and bZIP G-box Binding Factors (GBFs). The mechanisms of ... ...
| Abstract | Environmental control of the alcohol dehydrogenase (Adh) and other stress response genes in plants is in part brought about by transcriptional regulation involving the G-box cis-acting DNA element and bZIP G-box Binding Factors (GBFs). The mechanisms of GBF regulation and requirements for additional factors in this control process are not well understood. In an effort to identify potential GBF binding and control partners, maize GBF1 was used as bait in a yeast two-hybrid screen of an A. thaliana cDNA library. GBF Interacting Protein 1 (GIP1) arose from the screen as a 496 amino acid protein with a predicted molecular weight of 53,748 kDa that strongly interacts with GBFs. Northern analysis of A. thaliana tissue suggests a 1.8-1.9 kb GIP1 transcript, predominantly in roots. Immunolocalization studies indicate that GIP1 protein is mainly localized to the nucleus. In vitro electrophoretic mobility shift assays using an Adh G-box DNA probe and recombinant A. thaliana GBF3 or maize GBF1, showed that the presence of GIP1 resulted in a tenfold increase in GBF DNA binding activity without altering the migration, suggesting a transient association between GIP1 and GBF. Addition of GIP1 to intentionally aggregated GBF converted GBF to lower molecular weight macromolecular complexes and GIP1 also refolded denatured rhodanese in the absence of ATP. These data suggest GIP1 functions to enhance GBF DNA binding activity by acting as a potent nuclear chaperone or crowbar, and potentially regulates the multimeric state of GBFs, thereby contributing to bZIP-mediated gene regulation. |
|||||
|---|---|---|---|---|---|---|
| MeSH term(s) | Arabidopsis/metabolism ; Arabidopsis Proteins/chemistry ; Arabidopsis Proteins/metabolism ; Carrier Proteins/chemistry ; Carrier Proteins/metabolism ; Cell Nucleus/metabolism ; DNA/metabolism ; DNA, Complementary/genetics ; DNA-Binding Proteins/chemistry ; DNA-Binding Proteins/metabolism ; Electrophoretic Mobility Shift Assay ; Gene Expression Regulation, Plant ; Genomics ; Molecular Chaperones/metabolism ; Molecular Sequence Data ; Multiprotein Complexes/chemistry ; Multiprotein Complexes/metabolism ; Plant Roots/genetics ; Protein Binding ; Protein Structure, Quaternary ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/immunology ; Two-Hybrid System Techniques ; Zea mays | |||||
| Chemical Substances | Arabidopsis Proteins ; Carrier Proteins ; DNA, Complementary ; DNA-Binding Proteins ; GIP1 protein, Arabidopsis ; Molecular Chaperones ; Multiprotein Complexes ; RNA, Messenger ; Recombinant Fusion Proteins ; DNA (9007-49-2) | |||||
| Language | English | |||||
| Publishing date | 2005-08 | |||||
| Publishing country | England | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. | |||||
| ZDB-ID | 1319303-x | |||||
| ISSN | 1748-7838 ; 1001-0602 | |||||
| ISSN (online) | 1748-7838 | |||||
| ISSN | 1001-0602 | |||||
| DOI | 10.1038/sj.cr.7290326 | |||||
| Shelf mark |
|
|||||
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 5283: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.