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  1. Article ; Online: Quantitative proteomic analysis of bronchoalveolar lavage fluid in West Highland white terriers with canine idiopathic pulmonary fibrosis

    Maher, Rosemary E / Määttä, Merita / Beynon, R. J. / Laurila, Henna P / McNamara, Paul S / Rajamäki, Minna M

    BMC Vet Res. 2022 Dec., v. 18, no. 1 p.121-121

    2022  

    Abstract: BACKGROUND: Canine idiopathic pulmonary fibrosis (CIPF) is a chronic, progressive, interstitial fibrosing lung disease, manifesting as cough, exercise intolerance and ultimately, dyspnea and respiratory failure. It mainly affects West Highland white ... ...

    Abstract BACKGROUND: Canine idiopathic pulmonary fibrosis (CIPF) is a chronic, progressive, interstitial fibrosing lung disease, manifesting as cough, exercise intolerance and ultimately, dyspnea and respiratory failure. It mainly affects West Highland white terriers (WHWTs), lacks curable treatment and has a poor prognosis. Aspiration of gastroesophageal refluxate may play a role in the development of CIPF. In the first part of this study, we completed label-free quantitative proteomic analysis of bronchoalveolar lavage fluid (BALF) from CIPF and healthy WHWTs. In the second part, we evaluated potential protein markers of reflux aspiration from canine gastric juice and vomitus and whether these were present in BALF from the two groups. RESULTS: Across all BALF samples, 417 proteins were identified, and of these, 265 proteins were identified by two or more unique tryptic peptides. Using the 265 high confidence assignments, the quantitative proteome profiles were very similar in the two cohorts, but they could be readily resolved by principal component analysis on the basis of differential protein expression. Of the proteins that were differentially abundant in the two groups, several (including inflammatory and fibrotic markers) were elevated in CIPF, and a smaller, more diverse group of proteins were diminished in CIPF. No protein markers indicative of reflux aspiration were identified. CONCLUSIONS: Label-free proteomics allowed discrimination between CIPF and healthy WHWTs, consistent with fibrotic process but did not provide clear evidence for gastrointestinal aspiration. The measurement of proteins may provide a proteomics signature of CIPF that could be used to evaluate treatment options.
    Keywords cough ; dogs ; dyspnea ; exercise ; gastric juice ; gastrointestinal system ; peptides ; principal component analysis ; prognosis ; protein synthesis ; proteome ; proteomics ; pulmonary fibrosis
    Language English
    Dates of publication 2022-12
    Size p. 121.
    Publishing place BioMed Central
    Document type Article ; Online
    ZDB-ID 2191675-5
    ISSN 1746-6148
    ISSN 1746-6148
    DOI 10.1186/s12917-022-03202-x
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Quantitative proteomic analysis of bronchoalveolar lavage fluid in West Highland white terriers with canine idiopathic pulmonary fibrosis.

    Maher, Rosemary E / Määttä, Merita / Beynon, Robert J / Laurila, Henna P / McNamara, Paul S / Rajamäki, Minna M

    BMC veterinary research

    2022  Volume 18, Issue 1, Page(s) 121

    Abstract: Background: Canine idiopathic pulmonary fibrosis (CIPF) is a chronic, progressive, interstitial fibrosing lung disease, manifesting as cough, exercise intolerance and ultimately, dyspnea and respiratory failure. It mainly affects West Highland white ... ...

    Abstract Background: Canine idiopathic pulmonary fibrosis (CIPF) is a chronic, progressive, interstitial fibrosing lung disease, manifesting as cough, exercise intolerance and ultimately, dyspnea and respiratory failure. It mainly affects West Highland white terriers (WHWTs), lacks curable treatment and has a poor prognosis. Aspiration of gastroesophageal refluxate may play a role in the development of CIPF. In the first part of this study, we completed label-free quantitative proteomic analysis of bronchoalveolar lavage fluid (BALF) from CIPF and healthy WHWTs. In the second part, we evaluated potential protein markers of reflux aspiration from canine gastric juice and vomitus and whether these were present in BALF from the two groups.
    Results: Across all BALF samples, 417 proteins were identified, and of these, 265 proteins were identified by two or more unique tryptic peptides. Using the 265 high confidence assignments, the quantitative proteome profiles were very similar in the two cohorts, but they could be readily resolved by principal component analysis on the basis of differential protein expression. Of the proteins that were differentially abundant in the two groups, several (including inflammatory and fibrotic markers) were elevated in CIPF, and a smaller, more diverse group of proteins were diminished in CIPF. No protein markers indicative of reflux aspiration were identified.
    Conclusions: Label-free proteomics allowed discrimination between CIPF and healthy WHWTs, consistent with fibrotic process but did not provide clear evidence for gastrointestinal aspiration. The measurement of proteins may provide a proteomics signature of CIPF that could be used to evaluate treatment options.
    MeSH term(s) Animals ; Bronchoalveolar Lavage Fluid ; Dog Diseases/diagnosis ; Dog Diseases/metabolism ; Dogs ; Gastroesophageal Reflux/veterinary ; Idiopathic Pulmonary Fibrosis/veterinary ; Proteomics
    Language English
    Publishing date 2022-03-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 2191675-5
    ISSN 1746-6148 ; 1746-6148
    ISSN (online) 1746-6148
    ISSN 1746-6148
    DOI 10.1186/s12917-022-03202-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Characterisation of and risk factors for extended-spectrum β-lactamase producing Enterobacterales (ESBL-E) in an equine hospital with a special reference to an outbreak caused by Klebsiella pneumoniae ST307:CTX-M-1.

    Thomson, Katariina / Eskola, Katarina / Eklund, Marjut / Suominen, Kristiina / Määttä, Merita / Junnila, Jouni / Nykäsenoja, Suvi / Niinistö, Kati / Grönthal, Thomas / Rantala, Merja

    Acta veterinaria Scandinavica

    2022  Volume 64, Issue 1, Page(s) 4

    Abstract: Background: Extended-spectrum β-lactamase producing Enterobacterales (ESBL-E) are important causative agents for infections in humans and animals. At the Equine Veterinary Teaching Hospital of the University of Helsinki, the first infections caused by ... ...

    Abstract Background: Extended-spectrum β-lactamase producing Enterobacterales (ESBL-E) are important causative agents for infections in humans and animals. At the Equine Veterinary Teaching Hospital of the University of Helsinki, the first infections caused by ESBL-E were observed at the end of 2011 leading to enhanced infection surveillance. Contact patients were screened for ESBL-E by culturing infection sites and rectal screening. This study was focused on describing the epidemiology and microbiological characteristics of ESBL-E from equine patients of the EVTH during 2011-2014, and analysing putative risk factors for being positive for ESBL-E during an outbreak of Klebsiella pneumoniae ST307.
    Results: The number of ESBL-E isolations increased through 2012-2013 culminating in an outbreak of multi-drug resistant K. pneumoniae ST307:bla
    Conclusions: The occurrence of an outbreak caused by a pathogenic ESBL-producing K. pneumoniae ST307 strain highlights the importance of epidemiological surveillance of ESBL-E in veterinary hospitals. Limiting the length of hospitalisation for equine patients may reduce the risk of spread of ESBL-E. It is also important to acknowledge the importance of nasogastric tubing as a potential source of acquiring ESBL-E. As ESBL-E were also found in stomach drench pumps used with nasogastric tubes, veterinary practices should pay close attention to appropriate equipment cleaning procedures and disinfection practices.
    MeSH term(s) Animals ; Anti-Bacterial Agents/therapeutic use ; Disease Outbreaks/veterinary ; Female ; Horse Diseases/drug therapy ; Horse Diseases/epidemiology ; Horses ; Hospitals, Animal ; Hospitals, Teaching ; Humans ; Klebsiella Infections/drug therapy ; Klebsiella Infections/epidemiology ; Klebsiella Infections/veterinary ; Klebsiella pneumoniae ; Microbial Sensitivity Tests/veterinary ; Risk Factors ; beta-Lactamases
    Chemical Substances Anti-Bacterial Agents ; beta-lactamase TEM-3 (EC 3.5.2.-) ; beta-Lactamases (EC 3.5.2.6)
    Language English
    Publishing date 2022-02-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 40137-7
    ISSN 1751-0147 ; 0044-605X
    ISSN (online) 1751-0147
    ISSN 0044-605X
    DOI 10.1186/s13028-022-00621-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 484: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  4. Article: Matrix metalloproteinase‐2, ‐7, and ‐9 activities in dogs with idiopathic pulmonary fibrosis compared to healthy dogs and dogs with other respiratory diseases

    Määttä, Merita / Laurila, Henna P / Holopainen, Saila / Aaltonen, Kaisa / Lilja‐Maula, Liisa / Viitanen, Sanna / Rajamäki, Minna M

    Journal of veterinary internal medicine. 2021 Jan., v. 35, no. 1

    2021  

    Abstract: BACKGROUND: Canine idiopathic pulmonary fibrosis (CIPF) is a chronic, interstitial lung disease that mainly affects West Highland white terriers (WHWTs) and is characterized by excessive deposition of extracellular matrix (ECM) in the lung. Matrix ... ...

    Abstract BACKGROUND: Canine idiopathic pulmonary fibrosis (CIPF) is a chronic, interstitial lung disease that mainly affects West Highland white terriers (WHWTs) and is characterized by excessive deposition of extracellular matrix (ECM) in the lung. Matrix metalloproteinases (MMPs) participate in remodeling of ECM. OBJECTIVES: To compare metalloproteinase‐2, ‐7 and ‐9 activities in blood or bronchoalveolar lavage fluid (BALF) samples or both of CIPF WHWTs with healthy WHWTs, healthy dogs of other breeds, and dogs with other lung diseases and determine if these MMPs could be used as diagnostic and prognostic markers for CIPF. ANIMALS: Forty‐four CIPF WHWTs, 24 dogs with chronic bronchitis (CB), 17 with eosinophilic bronchopneumopathy (EBP), 10 with bacterial pneumonia, 39 healthy WHWTs, and 35 healthy dogs of other breeds. METHODS: Cross‐sectional observational study. Pro‐MMP and active MMP activities were analyzed by zymography. RESULTS: In serum, significantly higher (P < .01) pro‐MMP‐7 activities were observed in CIPF WHWTs compared to healthy dogs of other breeds, dogs with CB and dogs with EBP. In BALF of CIPF WHWTs, both pro‐MMP‐9 and pro‐MMP‐2 activities were significantly higher (P < .01) compared to healthy WHWTs, but these differences were not detected in plasma. The CIPF WHWTs had significantly higher (P < .05) activities of pro‐MMP‐9 compared to dogs with CB and of pro‐MMP‐2 compared to dogs with CB and EBP. No statistically significant prognostic factors were observed in CIPF WHWTs. CONCLUSIONS AND CLINICAL RELEVANCE: Serum MMP‐7 and BALF MMP‐2 and ‐9 potentially may be useful diagnostic markers but not prognostic markers for CIPF.
    Keywords bacterial pneumonia ; blood serum ; bronchitis ; dogs ; extracellular matrix ; gelatinase A ; lungs ; observational studies ; pulmonary fibrosis ; veterinary medicine
    Language English
    Dates of publication 2021-01
    Size p. 462-471.
    Publishing place John Wiley & Sons, Inc.
    Document type Article
    Note NAL-AP-2-clean ; JOURNAL ARTICLE
    ZDB-ID 92798-3
    ISSN 1939-1676 ; 0891-6640
    ISSN (online) 1939-1676
    ISSN 0891-6640
    DOI 10.1111/jvim.15970
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4095: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Characterisation of and risk factors for extended-spectrum β-lactamase producing Enterobacterales (ESBL-E) in an equine hospital with a special reference to an outbreak caused by Klebsiella pneumoniae ST307:CTX-M-1

    Thomson, Katariina / Eskola, Katarina / Eklund, Marjut / Suominen, Kristiina / Määttä, Merita / Junnila, Jouni / Nykäsenoja, Suvi / Niinistö, Kati / Grönthal, Thomas / Rantala, Merja

    Acta veterinaria scandinavica. 2022 Dec., v. 64, no. 1

    2022  

    Abstract: BACKGROUND: Extended-spectrum β-lactamase producing Enterobacterales (ESBL-E) are important causative agents for infections in humans and animals. At the Equine Veterinary Teaching Hospital of the University of Helsinki, the first infections caused by ... ...

    Abstract BACKGROUND: Extended-spectrum β-lactamase producing Enterobacterales (ESBL-E) are important causative agents for infections in humans and animals. At the Equine Veterinary Teaching Hospital of the University of Helsinki, the first infections caused by ESBL-E were observed at the end of 2011 leading to enhanced infection surveillance. Contact patients were screened for ESBL-E by culturing infection sites and rectal screening. This study was focused on describing the epidemiology and microbiological characteristics of ESBL-E from equine patients of the EVTH during 2011–2014, and analysing putative risk factors for being positive for ESBL-E during an outbreak of Klebsiella pneumoniae ST307. RESULTS: The number of ESBL-E isolations increased through 2012–2013 culminating in an outbreak of multi-drug resistant K. pneumoniae ST307:blaCTX₋M₋₁:blaTEM:blaSHV during 04–08/2013. During 10/2011–05/2014, altogether 139 ESBL-E isolates were found from 96 horses. Of these, 26 were from infection-site specimens and 113 from rectal-screening swabs. A total of 118 ESBL-E isolates from horses were available for further study, the most numerous being K. pneumoniae (n = 44), Escherichia coli (n = 31) and Enterobacter cloacae (n = 31). Hospital environmental specimens (N = 47) yielded six isolates of ESBL-E. Two identical E. cloacae isolates originating from an operating theatre and a recovery room had identical or highly similar PFGE fingerprint profiles as five horse isolates. In the multivariable analysis, mare–foal pairs (OR 4.71, 95% CI 1.57–14.19, P = 0.006), length of hospitalisation (OR 1.62, 95% CI 1.28–2.06, P < 0.001) and passing of a nasogastric tube (OR 2.86, 95% CI 1.03–7.95, P = 0.044) were associated with being positive for ESBL-E during the K. pneumoniae outbreak. CONCLUSIONS: The occurrence of an outbreak caused by a pathogenic ESBL-producing K. pneumoniae ST307 strain highlights the importance of epidemiological surveillance of ESBL-E in veterinary hospitals. Limiting the length of hospitalisation for equine patients may reduce the risk of spread of ESBL-E. It is also important to acknowledge the importance of nasogastric tubing as a potential source of acquiring ESBL-E. As ESBL-E were also found in stomach drench pumps used with nasogastric tubes, veterinary practices should pay close attention to appropriate equipment cleaning procedures and disinfection practices.
    Keywords Enterobacter cloacae ; Escherichia coli ; Klebsiella pneumoniae ; beta-lactamase bacteria ; disinfection ; epidemiology ; horses ; hospitals ; monitoring ; multiple drug resistance ; risk reduction ; stomach
    Language English
    Dates of publication 2022-12
    Size p. 4.
    Publishing place BioMed Central
    Document type Article
    ZDB-ID 40137-7
    ISSN 1751-0147 ; 0044-605X
    ISSN (online) 1751-0147
    ISSN 0044-605X
    DOI 10.1186/s13028-022-00621-6
    Database NAL-Catalogue (AGRICOLA)

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    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 484: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Matrix metalloproteinase-2, -7, and -9 activities in dogs with idiopathic pulmonary fibrosis compared to healthy dogs and dogs with other respiratory diseases.

    Määttä, Merita / Laurila, Henna P / Holopainen, Saila / Aaltonen, Kaisa / Lilja-Maula, Liisa / Viitanen, Sanna / Rajamäki, Minna M

    Journal of veterinary internal medicine

    2020  Volume 35, Issue 1, Page(s) 462–471

    Abstract: Background: Canine idiopathic pulmonary fibrosis (CIPF) is a chronic, interstitial lung disease that mainly affects West Highland white terriers (WHWTs) and is characterized by excessive deposition of extracellular matrix (ECM) in the lung. Matrix ... ...

    Abstract Background: Canine idiopathic pulmonary fibrosis (CIPF) is a chronic, interstitial lung disease that mainly affects West Highland white terriers (WHWTs) and is characterized by excessive deposition of extracellular matrix (ECM) in the lung. Matrix metalloproteinases (MMPs) participate in remodeling of ECM.
    Objectives: To compare metalloproteinase-2, -7 and -9 activities in blood or bronchoalveolar lavage fluid (BALF) samples or both of CIPF WHWTs with healthy WHWTs, healthy dogs of other breeds, and dogs with other lung diseases and determine if these MMPs could be used as diagnostic and prognostic markers for CIPF.
    Animals: Forty-four CIPF WHWTs, 24 dogs with chronic bronchitis (CB), 17 with eosinophilic bronchopneumopathy (EBP), 10 with bacterial pneumonia, 39 healthy WHWTs, and 35 healthy dogs of other breeds.
    Methods: Cross-sectional observational study. Pro-MMP and active MMP activities were analyzed by zymography.
    Results: In serum, significantly higher (P < .01) pro-MMP-7 activities were observed in CIPF WHWTs compared to healthy dogs of other breeds, dogs with CB and dogs with EBP. In BALF of CIPF WHWTs, both pro-MMP-9 and pro-MMP-2 activities were significantly higher (P < .01) compared to healthy WHWTs, but these differences were not detected in plasma. The CIPF WHWTs had significantly higher (P < .05) activities of pro-MMP-9 compared to dogs with CB and of pro-MMP-2 compared to dogs with CB and EBP. No statistically significant prognostic factors were observed in CIPF WHWTs.
    Conclusions and clinical relevance: Serum MMP-7 and BALF MMP-2 and -9 potentially may be useful diagnostic markers but not prognostic markers for CIPF.
    MeSH term(s) Animals ; Bronchoalveolar Lavage Fluid ; Cross-Sectional Studies ; Dog Diseases ; Dogs ; Idiopathic Pulmonary Fibrosis/veterinary ; Lung ; Matrix Metalloproteinase 2 ; Matrix Metalloproteinase 7 ; Matrix Metalloproteinase 9
    Chemical Substances Matrix Metalloproteinase 7 (EC 3.4.24.23) ; Matrix Metalloproteinase 2 (EC 3.4.24.24) ; Matrix Metalloproteinase 9 (EC 3.4.24.35)
    Language English
    Publishing date 2020-12-04
    Publishing country United States
    Document type Journal Article ; Observational Study, Veterinary
    ZDB-ID 92798-3
    ISSN 1939-1676 ; 0891-6640
    ISSN (online) 1939-1676
    ISSN 0891-6640
    DOI 10.1111/jvim.15970
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4095: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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