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  1. Book ; Thesis: Support of kinetochore microtubule attachments by CENP-V is required for meiosis progression in mouse oocytes

    Nabi, Dalileh / Jessberger, Rolf / Müller-Reichert, Thomas

    2021  

    Institution Institut für Physiologische Chemie
    Author's details von Dalileh Nabi, M.Sc. aus Teheran, Iran ; Institut für Physiologische Chemie [Dresden]
    Language English
    Size vii, 81 Blätter, Illustrationen, Diagramme
    Publishing place Dresden
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Dissertation, Technische Universität Dresden, Medizinische Fakultät, 2021
    HBZ-ID HT021013541
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2021 E 1004 order for loan Magazin

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  2. Book ; Thesis: Ultrastructural characterization of mammalian k-fibers by large-scale electron tomography

    Kiewisz, Robert / Müller-Reichert, Thomas / Needleman, Daniel J.

    2021  

    Institution Medizinische Klinik und Poliklinik III
    Author's details von Robert Kiewisz aus Olsztyn, Polen ; Medizinische Klinik und Poliklinik III [Dresden]
    Language English
    Size 153 Blätter, Illustrationen, Diagramme
    Publishing place Dresden
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Dissertation, Technische Universität Dresden, Medizinische Fakultät, 2021
    HBZ-ID HT021771588
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2023 E 595 order for loan Magazin

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  3. Book ; Online ; E-Book: Three-dimensional electron microscopy

    Müller-Reichert, Thomas / Pigino, Gaia

    (Methods in cell biology ; 152)

    2019  

    Author's details edited by Thomas Muller-Reichert, Gaia Pigino
    Series title Methods in cell biology ; 152
    Collection
    Keywords Electron microscopy ; Three-dimensional imaging in biology
    Subject code 570.2825
    Language English
    Size 1 Online-Ressource (xiii, 289 Seiten), Illustrationen, Diagramme
    Edition First edition
    Publisher Elsevier AP Academic Press
    Publishing place Cambridge, MA
    Publishing country United States
    Document type Book ; Online ; E-Book
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT020205760
    ISBN 978-0-12-817019-9 ; 9780128170182 ; 0-12-817019-0 ; 0128170182
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

    Full text online

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  4. Book ; Thesis: Development and application of image processing algorithms for the analysis of renal cell populations and function in mouse models

    Kessel, Friederike / Hugo, Christian / Müller-Reichert, Thomas

    2021  

    Institution Medizinische Klinik und Poliklinik III
    Author's details von M.Sc. Friederike Kessel aus Wangen im Allgäu ; Medizinische Klinik und Poliklinik III [Dresden]
    Language English
    Size 95 Seiten, Illustrationen, Diagramme
    Publishing place Dresden
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Dissertation, Technische Universität Dresden, Medizinische Fakultät, 2022
    HBZ-ID HT021777260
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2023 E 639 order for loan Magazin

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  5. Book: Correlative light and electron microscopy

    Müller-Reichert, Thomas

    (Methods in cell biology ; 111)

    2012  

    Author's details ed. by Thomas Müller-Reichert
    Series title Methods in cell biology ; 111
    Collection
    Keywords Microscopy ; Electron microscopy ; Molecular biology
    Subject code 570.2823
    Language English
    Size XIX, 404 S., [20] Bl. : Ill., graph. Darst., 24 cm
    Edition 1. ed.
    Publisher Elsevier Acad. Press
    Publishing place Amsterdam u.a.
    Publishing country Netherlands
    Document type Book
    HBZ-ID HT017419107
    ISBN 978-0-12-416026-2 ; 0-12-416026-3
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2012 A 4124 order for loan Magazin

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  6. Book: Electron microscopy of model systems

    Müller-Reichert, Thomas

    (Methods in cell biology ; 96)

    2010  

    Author's details ed. by Thomas Müller-Reichert
    Series title Methods in cell biology ; 96
    Collection
    Language English
    Size XX, 724 S., [18] Bl. : Ill., graph. Darst., 26 cm
    Edition 1. ed.
    Publisher Elsevier, Acad. Press
    Publishing place Amsterdam u.a.
    Publishing country Netherlands
    Document type Book
    HBZ-ID HT016545432
    ISBN 978-0-12-381007-6 ; 0-12-381007-8
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2010 A 5125 order for loan Magazin

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  7. Article: How to apply the broad toolbox of correlative light and electron microscopy to address a specific biological question.

    Tranfield, Erin M / Fabig, Gunar / Kurth, Thomas / Müller-Reichert, Thomas

    Methods in cell biology

    2024  Volume 187, Page(s) 1–41

    Abstract: Correlative light and electron microscopy (CLEM) is an approach that combines the strength of multiple imaging techniques to obtain complementary information about a given specimen. The "toolbox" for CLEM is broad, making it sometimes difficult to choose ...

    Abstract Correlative light and electron microscopy (CLEM) is an approach that combines the strength of multiple imaging techniques to obtain complementary information about a given specimen. The "toolbox" for CLEM is broad, making it sometimes difficult to choose an appropriate approach for a given biological question. In this chapter, we provide experimental details for three CLEM approaches that can help the interested reader in designing a personalized CLEM strategy for obtaining ultrastructural data by using transmission electron microscopy (TEM). First, we describe chemical fixation of cells grown on a solid support (broadest approach). Second, we apply high-pressure freezing/freeze substitution to describe cellular ultrastructure (cryo-immobilization approach). Third, we give a protocol for a ultrastructural labeling by immuno-electron microscopy (immuno-EM approach). In addition, we also describe how to overlay fluorescence and electron microscopy images, an approach that is applicable to each of the reported different CLEM strategies. Here we provide step-by step descriptions prior to discussing possible technical problems and variations of these three general schemes to suit different models or different biological questions. This chapter is written for electron microscopists that are new to CLEM and unsure how to begin. Therefore, our protocols are meant to provide basic information with further references that should help the reader get started with applying a tailored strategy for a specific CLEM experiment.
    MeSH term(s) Humans ; Microscopy, Electron, Transmission/methods ; Animals ; Cryoelectron Microscopy/methods ; Microscopy, Electron/methods ; Microscopy, Immunoelectron/methods ; Microscopy, Fluorescence/methods ; Freeze Substitution/methods
    Language English
    Publishing date 2024-03-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 0091-679X
    ISSN 0091-679X
    DOI 10.1016/bs.mcb.2024.02.030
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The X chromosome still has a lot to reveal - revisiting Hermann Henking's work on firebugs.

    Paliulis, Leocadia / Fabig, Gunar / Müller-Reichert, Thomas

    Journal of cell science

    2023  Volume 136, Issue 4

    Abstract: In 1891, the existence of an X chromosome was noted for the first time. Hermann Henking was studying spermatocyte divisions of the firebug Pyrrhocoris apterus and observed that one chromosome behaved differently than all of the rest of the chromosomes. ... ...

    Abstract In 1891, the existence of an X chromosome was noted for the first time. Hermann Henking was studying spermatocyte divisions of the firebug Pyrrhocoris apterus and observed that one chromosome behaved differently than all of the rest of the chromosomes. Henking called this chromosome 'Element x'. Henking's discovery of the X element (later called X chromosome) initiated more than a century of fascinating genetics and cell biology, forming the foundation of several avenues of research in biology. His work led to exploration of a number of questions in a wide range of model systems and very soon to the abandonment of the firebug as a model for studies on the behavior of chromosomes in meiosis. Here, we argue that studies on both bivalent and univalent chromosome behavior in general, and work on how to solve chromosome lagging to prevent aneuploidy in particular, should lead us back to using the firebug as a model for error correction during cell division.
    MeSH term(s) Humans ; Meiosis ; Chromosomes/genetics ; Aneuploidy
    Language English
    Publishing date 2023-02-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 2993-2
    ISSN 1477-9137 ; 0021-9533
    ISSN (online) 1477-9137
    ISSN 0021-9533
    DOI 10.1242/jcs.260998
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  9. Article ; Online: Serial-section Electron Tomography and Quantitative Analysis of Microtubule Organization in 3D-reconstructed Mitotic Spindles.

    Kiewisz, Robert / Baum, Daniel / Müller-Reichert, Thomas / Fabig, Gunar

    Bio-protocol

    2023  Volume 13, Issue 20, Page(s) e4849

    Abstract: For the analysis of cellular architecture during mitosis, nanometer resolution is needed to visualize the organization of microtubules in spindles. Here, we present a detailed protocol that can be used to produce 3D reconstructions of whole mitotic ... ...

    Abstract For the analysis of cellular architecture during mitosis, nanometer resolution is needed to visualize the organization of microtubules in spindles. Here, we present a detailed protocol that can be used to produce 3D reconstructions of whole mitotic spindles in cells grown in culture. For this, we attach mammalian cells enriched in mitotic stages to sapphire discs. Our protocol further involves cryo-immobilization by high-pressure freezing, freeze-substitution, and resin embedding. We then use fluorescence light microscopy to stage select mitotic cells in the resin-embedded samples. This is followed by large-scale electron tomography to reconstruct the selected and staged mitotic spindles in 3D. The generated and stitched electron tomograms are then used to semi-automatically segment the microtubules for subsequent quantitative analysis of spindle organization. Thus, by providing a detailed correlative light and electron microscopy (CLEM) approach, we give cell biologists a toolset to streamline the 3D visualization and analysis of spindle microtubules (http://kiewisz.shinyapps.io/asga). In addition, we refer to a recently launched platform that allows for an interactive display of the 3D-reconstructed mitotic spindles (https://cfci.shinyapps.io/ASGA_3DViewer/). Key features • High-throughput screening of mitotic cells by correlative light and electron microscopy (CLEM). • Serial-section electron tomography of selected cells. • Visualization of mitotic spindles in 3D and quantitative analysis of microtubule organization.
    Language English
    Publishing date 2023-10-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.4849
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Automated Segmentation of 3D Cytoskeletal Filaments from Electron Micrographs with TARDIS.

    Kiewisz, Robert / Fabig, Gunar / Müller-Reichert, Thomas / Bepler, Tristan

    Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

    2023  Volume 29, Issue 29 Suppl 1, Page(s) 970–972

    Language English
    Publishing date 2023-08-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1385710-1
    ISSN 1435-8115 ; 1431-9276
    ISSN (online) 1435-8115
    ISSN 1431-9276
    DOI 10.1093/micmic/ozad067.485
    Database MEDical Literature Analysis and Retrieval System OnLINE

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